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SECTION 3 - THE NERVOUS SYSTEM AND SENSORY PHYSIOLOGY EXERCISE 3.1
RECORDING THE NERVE ACTION POTENTIAL
Approximate Time for Completion: 1–2 hours Introduction This exercise is designed to introduce students to action potentials, to the activity of a living nerve, and to the oscilloscope. Action potentials are often difficult for students to understand, so an explanation prior to the laboratory period is essential. This exercise may be performed as a demonstration and supported by films or CD-ROMs. A description of the procedure, the experiment purpose, the setup, and the operation of the equipment is necessary and will take time. Performing the frog dissection before lab will provide more time for discussion.
Materials 1. Frogs 2. Dissecting equipment and trays, glass probes, thread 3. Oscilloscope, or computer, and nerve chamber 4. Frog Ringer’s solution (see Materials, exercise 5.1) 5. Alternatively, labs with more advanced equipment can measure the action potential in a single axon, using other animals, such as crayfish (crawdads). For example, see Crawdad Project at www.crawdad.cornell.edu.
Textbook Correlations: Chapter 7 – Action Potentials; Conduction of Nerve Impulses Clinical Investigations 1.
Describe the role of plasma membrane Na+ and K+ channels in the production of action potentials. Na+ are in the extracellular area while K+ are in the intracellular area. Upon stimulation, the voltage channels (in this case the sodium channels) will open thus allowing Na + to enter the neuron (intracellular area). This is depolarization, the start of an impulse. The entrance of Na + into the neuron changes the voltage from -70mV to +30mV. This voltage change causes the next ion channel to open to let more Na+ enter (and this process continues in a domino fashion down the axon to the destination). This action manifests itself as a “moving impulse.” While the Na+ are entering the neuron the K+ channels are opening to allow K+ ions to exit the neuron in an effort to maintain stabilized ionic charges in the ICF and the ECF. 1
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