OPERATING INSTRUCTIONS
SlimlineTM
Printed in U.S.A.
Fixed-Intensity Ultraviolet Transilluminator
Manual No. AM07024-2 Issued: December 2008
DANGER Ultraviolet radiation emitted from this product. Avoid exposure. ALWAYS WEAR PROTECTIVE CLOTHING. EXPOSURE MAY CAUSE PREMATURE AGING OF THE SKIN AND CANCER. ALWAYS WEAR PROTECTIVE EYEWEAR; FAILURE TO DO SO MAY RESULT IN SEVERE BURNS OR LONG TERM INJURY TO EYE. Never look directly into the lamp. Exposure can cause eye and skin allergy and allergic reactions. Medications or cosmetics may increase your sensitivity to ultraviolet radiation. Consult physician before operating this product if you are using medications or have a history of skin problems or believe yourself especially sensitive to sunlight. For Laboratory Use Only
WARNING Please read the instructions in this manual carefully before using your Spectroline® transilluminator. Never use this equipment in any manner not specified in these instructions because the protection provided by the equipment may be impaired. We recommend using Spectroline UV-absorbing protective eye and face wear. See Replacement Parts and Accessories for model numbers. This eye and face wear also improves contrast between the fluorescent area and the background and eliminates ‘‘blue haze’’ interference. INTRODUCTION TM
Spectroline Slimline series ultraviolet transilluminators produce the high irradiance needed to excite fluorescence in electrophoresis gels and thin layer chromatograms. They are also ideal for use in forensic science, pharmaceutical quality control, and much more. Each unit offers high UV intensity, uniform irradiance and cool operation. Each unit is equipped with a special, polymer-based diffusing screen that ensures excellent irradiance uniformity at the filter surface and improves photographic quality. These fixed-intensity ultraviolet transilluminators are available in 120V/60Hz, 230V/50Hz or 100V/50-60Hz versions.
DIMENSIONS AND SPECIFICATIONS Width Length Depth Wavelengths Filter Size Equipment
11.50 in 29.2cm 14.0 in 35.6cm 5.7 in 14.5cm 254nm, 312nm and 365nm 4.3 x 5.5 in 11 x 14cm Four 8-watt tubes (UV-A, UV-B or UV-C), UV-blocking cover, diffusing screen (except 254nm), on/off switch
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OPERATION 1. Carefully unpack and inspect the transilluminator for possible damage in shipment. If any damage is noted, immediately notify the carrier and supplier and do not operate the unit. 2. Unwrap the hinged UV-blocking cover. Remove the four Phillips screws from the top of the filter assembly. Attach the hinged cover to the filter assembly with the four screws. Adjusting the screw in the center of each hinge assembly on the cover maintains the proper hinge tension so the cover can be suspended o o at any angle from 0 to 180 . 3. Connect the plug of the cord first to the fused power entry unit and then to a power outlet that complies with the electrical requirements specified on the unit’s label. 4. Press the switch to turn on the unit. View the filter area through the UV blocking cover to check for a steady glow of light. If the unit is cold, the light may initially flicker, but it should stabilize after a few seconds.
DIFFUSING SCREEN The Slimline models are equipped with a special diffusing screen attached to the underside of the UV filter. (The 254nm models do not have a diffusing screen.) This screen ensures excellent irradiance uniformity across the filter area and eliminates light striations. This feature is especially important in photographic applications. NOTE: The diffusing screen lowers the overall UV intensity of the 312nm and 365nm models by approximately 30%.
REMOVAL/REPLACEMENT OF DIFFUSING SCREEN 1. Remove the filter assembly as described in Replacing the Filter Assembly. 2. Carefully place the filter assembly filter-side down on a flat, level surface, making sure that it does not get scratched. 3. Remove the four screws and lift the diffusing screen off the filter assembly. If the diffusing screen is going to be used again, store the screen and screws. 4. Reinstall/replace the diffusing screen by centering its holes over the screws protruding from the filter assembly. Be sure the metal frame of the screen is facing toward the tubes (light source). 5. Tighten the four screws and reinstall the filter assembly on the transilluminator.
Caution (refer to accompanying documents)
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REPLACING THE FILTER ASSEMBLY After considerable use, the filter on the transilluminator may solarize and require replacement. Disconnect the cord from the unit. Remove the four screws to take off the UV-blocking cover from the filter assembly. Remove the six screws holding the filter assembly and retain them. Remove the diffusing screen (see Removal/ Replacement of Diffusing Screen). Reattach the diffusing screen to the new filter assembly. Tighten the six screws on the new filter assembly and reattach the UV-blocking cover.
UV-TRANSMITTING FILTER PROTECTOR To facilitate cutting of gels without damage to the filter glass, the UV-transmitting filter protector is available separately. This accessory also protects the filter from damage caused by accidentally dropping small objects on the transilluminator. In addition, since buffer and ethidium bromide will not come in contact with the filter, it eliminates clouding of the glass and high background fluorescence. To use, set the filter protector directly on the transilluminator’s filter assembly. Then place the sample on the surface of the filter protector. Please note that the filter protector cannot be used with short wave UV transilluminators since it does not transmit 254nm.
SPECTROLINE UV VIEWING CABINETS Spectroline UV viewing cabinets provide increased fluorescent contrast of samples and eliminate the need for separate UV protection. The sample is placed on the transilluminator filter and safely observed through the UV-absorbing viewing window of the cabinet. The TE model transilluminators are compatible with the Spectroline CC-80/CC-81, CM-10 series and CX-20/CX-21 UV viewing cabinets.
REPLACING THE UV TUBES To replace the ultraviolet tube(s), remove the six screws holding the filter assembly to the transilluminator housing. If visually inspecting lit tubes, wear a Spectroline UVF-80 UV-absorbing face shield and protective clothing. Check for cracked or malfunctioning tubes. Replace a suspected malfunctioning tube with a new tube. If the new tube performs properly, discard the old tube. NOTE: Use a clean cloth or wear cotton gloves during servicing. Contamination may result if the glass section of the UV tubes or the metal reflector is touched directly. This will reduce the UV output. After servicing, wipe the tubes clean with a pure solvent (such as methanol) or mild detergent to eliminate contamination that could cause damage.
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Once all malfunctioning tubes are replaced, lower the filter assembly to its original position. Replace the six screws that hold the filter frame to the housing and replace the UV-blocking cover.
CLEANING PROCEDURE Immediately clean all spilled materials from the transilluminator and wipe dry. If necessary, moisten a cloth with soap and water and clean the transilluminator. Do not use any harsh chemical cleaners. Do not attempt to clean the unit while it is plugged into a power source.
TROUBLESHOOTING If the transilluminator does not yield satisfactory results, check that the fuse is operating properly. After removing cord from power source, use needlenose pliers to remove the fuse drawer from the power entry unit. There is an extra fuse in the 120V and 100V units. Remove and discard the spent fuse and replace it with the spare. The fuse in the 230V unit has two active fuses and a filter. Both fuses must be replaced. Use 1.25A, 250V, Fast Blo metric fuses for the 120V, 230V and 100V units. Symptom 1. Low output from part of filter area 2. Low uniform output
Probable Cause UV tube(s) functioning at substandard intensity level a. Filter solarized b. UV tubes functioning at substandard level c. Diffusing screen solarized
Cure Replace tube(s) a. Replace filter assembly b. Replace tubes c. Replace diffusing screen
ELECTRICAL SPECIFICATIONS MODEL TE-254S TE-254S/F TE-254S/J TE-312S TE-312S/F TE-312S/J TE-365S TE-365S/F TE-365S/J
WAVELENGTH UV-C UV-C UV-C UV-B UV-B UV-B UV-A UV-A UV-A
VOLTS 120 230 100 120 230 100 120 230 100
HZ 60 50 50-60 60 50 50-60 60 50 50-60
AMPS 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0 1.0
FUSE 1.25A F 1.25A F 1.25A F 1.25A F 1.25A F 1.25A F 1.25A F 1.25A F 1.25A F
Long Wave (UV-A): 365nm; Medium Wave (UV-B): 312nm; Short Wave (UV-C): 254nm
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ENVIRONMENTAL CONDITIONS The Spectroline Slimline fixed-intensity ultraviolet transilluminators are designed to be safe under the following conditions. • Indoor use; • Altitude up to 2,000 m (6,562 ft); • Temperature 5°C to 40°C (41°F to 104°F); • Maximum relative humidity 80% for temperatures up to 31°C (88°F) decreasing linearly to 50% relative humidity at 40°C (104°F); • Mains supply voltage fluctuations not to exceed ±10% of the nominal voltage; • Installation Category II; • Pollution Degree 2.
WARRANTY The warranty policy for the transilluminator is provided on the Certificate of Limited Warranty enclosed separately with each unit.
NOTE: For assistance of any kind, contact the Customer Service Department at Spectronics Corporation. In the U.S. and Canada, call toll-free 1-800-274-8888. Give full details of the difficulty and include the model and serial numbers of the unit and the date of purchase. If the transilluminator, or any components, must be returned to the factory, shipping instructions will be provided. If an estimate of charges for nonwarranty work or other service work is required, a quote will be furnished upon evaluation of the unit. Out-of-service work will not be performed without customer approval.
SHIPPING Carefully pack the unit in the original shipping container and packing materials. Ship it prepaid to the factory. Insure it for full value.
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LAMP CONTAINS MERCURY Manage in Accord with Disposal Laws See: www.lamprecycle.org
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REPLACEMENT PARTS AND ACCESSORIES DESCRIPTION Tube, Short Wave, 254nm Tube, Medium Wave, 312nm Tube, Long Wave, 365nm Diffusing Screen, 312nm and 365nm Filter Assembly, Short Wave, 254nm Filter Assembly, Medium Wave, 312nm Filter Assembly, Long Wave, 365nm Fuse, 1.25 Amp, 250V, Fast Blo Metric Hinged UV-Blocking Cover Mounting Hinge for UVC-100A UV-Transmitting Filter Protector Spectacles, UV-Absorbing Goggles, UV-Absorbing Face Shield, UV-Absorbing
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PART NO. BLE-8T254 BLE-8T312 BLE-8T365 108237 2F405 3F405S 2F685 100460 UVC-100A PC-0199 UVT-75A UVS-30 UVG-50 UVF-80