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Molecular Pathology  

Molecular Biology  Principles  

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Molecular Pathology  

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The nascent chain (run-on) assay allows measurement of the rate of transcription of a given gene!

Molecular Biology  Principles  

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Molecular Pathology  

Molecular Biology  Principles  

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Molecular Pathology  

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I: pre-rRNA! II: mRNA! III: tRNAs, 5S rRNA, ! small stable RNAs!

Repeat = Tyr-Ser-Pro-Thr-Ser-Pro-Ser!

Molecular Biology  Principles  

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Molecular Pathology  

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Alternative promoters in eukaryotes include initiators and CpG islands!

Molecular Biology  Principles  

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Molecular Pathology  

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Identification of the SV40 enhancer region!

Molecular Biology  Principles  

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Molecular Pathology  

Molecular Biology  Principles  

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Molecular Pathology  

Molecular Biology  Principles  

h/p://www.interpathology.org

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Molecular Pathology  

Molecular Biology  Principles  

h/p://www.interpathology.org

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Molecular Pathology  

Molecular Biology  Principles  

h/p://www.interpathology.org

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Molecular Pathology  

Molecular Biology  Principles  

h/p://www.interpathology.org

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Molecular Pathology  

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  Eukaryotic transcription is regulated by repressors as well as activators!   Repressor-binding sites can be identified and repressors purified by the same techniques used for activators!   Many eukaryotic repressors have two domains: a DNAbinding domain and a repressor domain!

Molecular Biology  Principles  

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Molecular Pathology  

Molecular Biology  Principles  

h/p://www.interpathology.org

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Molecular Pathology  

h/p://www.interpathology.org

Silent genes in mating-type control in yeast!

Molecular Biology  Principles  

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Molecular Pathology  

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Binding sites for activators that control transcription of the mouse TTR gene!

Molecular Biology  Principles  

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Molecular Pathology  

Molecular Biology  Principles  

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Molecular Pathology  

Molecular Biology  Principles  

h/p://www.interpathology.org

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Molecular Pathology  

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Model of IFN-mediated gene activation by phosphorylation and dimerization of Stat1!

Molecular Biology  Principles  

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Molecular Pathology  

h/p://www.interpathology.org

Copyright (c)  by  W.  H.  Freeman  and   Company  

Molecular Biology  Principles  

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RNA - Structure and function I  

Teaching program in Molecular Pathology. Principles of Molecular Biology - Initiation of transcription

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