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Molecular Pathology  

Molecular Biology  Principles  

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Molecular Pathology  

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Requires fluorescent ! tag for desired cell!

Molecular Biology  Principles  

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Molecular Pathology  

Molecular Biology  Principles  

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Molecular Pathology  

Molecular Biology  Principles  

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Molecular Pathology  

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The two faces of a membrane are asymmetric in terms of lipid and protein composition!

Molecular Biology  Principles  

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Molecular Pathology  

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“The Fluid Mosaic Model”!

Mobility (diffusion) of a given membrane components depends on:!   the size of the molecule!   its interactions with other molecules!   temperature!   lipid composition (tails, cholesterol)! Mobility can be measured by “FRAP”!

Molecular Biology  Principles  

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Molecular Pathology  

Molecular Biology  Principles  

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Molecular Pathology  

Molecular Biology  Principles  

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Molecular Pathology  

Molecular Biology  Principles  

h/p://www.interpathology.org

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Molecular Pathology  

Molecular Biology  Principles  

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Molecular Pathology  

Molecular Biology  Principles  

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Molecular Pathology  

Molecular Biology  Principles  

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Molecular Pathology  

Molecular Biology  Principles  

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Molecular Pathology  

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A, G, T, C are present in DNA! A, G, U, C are present in RNA !

Molecular Biology  Principles  

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Molecular Pathology  

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Hydrogen bonding between complementary base pairs (A-T or G-C) holds the two ! strands together!

Molecular Biology  Principles  

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Molecular Pathology  

Molecular Biology  Principles  

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Molecular Pathology  

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supercoiled!

Molecular Biology  Principles  

relaxed!

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Molecular Pathology  

Molecular Biology  Principles  

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Molecular Pathology  

Molecular Biology  Principles  

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Molecular Pathology  

Molecular Biology  Principles  

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Molecular Pathology  

Molecular Biology  Principles  

h/p://www.interpathology.org

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Molecular Pathology  

Molecular Biology  Principles  

h/p://www.interpathology.org

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Molecular Pathology  

Molecular Biology  Principles  

h/p://www.interpathology.org

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Molecular Pathology  

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2nd base   U  

1st base  –  3rd  base  in  each  row  

U

C

A

G

Molecular Biology  Principles  

C

Basic

G

Acidic

UUU (Phe/F)  

UCU (Ser/S)  

UAU (Tyr/T)  

A

UGU (Cys/C)  

Polar

UUC (Phe/F)  

UCC (Ser/S)  

UAC (Tyr/T)  

UGC (Cys/C)  

UUA (Leu/L)  

UCA (Ser/S)  

UAA -­‐  Ochre  (Stop)  

UGA -­‐  Opal  (Stop)  

UUG (Leu/L)  

UCG (Ser/S)  

UAG -­‐  Amber  (Stop)  

UGG (Trp/W)  

CUU (Leu/L)  

CCU (Pro/P)  

CAU (His/H)  

CGU (Arg/R)  

CUC (Leu/L)  

CCC (Pro/P)  

CAC (His/H)  

CGC (Arg/R)  

CUA (Leu/L)  

CCA (Pro/P)  

CAA (Gln/Q)  

CGA (Arg/R)  

CUG (Leu/L)  

CCG (Pro/P)  

CAG (Gln/Q)  

CGG (Arg/R)  

AUU (Ile/I)  

ACU (Thr/T)  

AAU (Asn/N)  

AGU (Ser/S)  

AUC (Ile/I)  

ACC (Thr/T)  

AAC (Asn/N)  

AGC (Ser/S)  

AUA (Ile/I)  

ACA (Thr/T)  

AAA (Lys/K)  

AGA (Arg/R)  

AUG (Met/M)  

ACG (Thr/T)  

AAG (Lys/K)  

AGG (Arg/R)  

GUU (Val/V)  

GCU (Ala/A)  

GAU (Asp/D)  

GGU (Gly/G)  

GUC (Val/V)  

GCC (Ala/A)  

GAC (Asp/D)  

GGC (Gly/G)  

GUA (Val/V)  

GCA (Ala/A)  

GAA (Glu/E)  

GGA (Gly/G)  

GUG (Val/V)  

GCG (Ala/A)  

GAG (Glu/E)  

GGG (Gly/G)  

Nonpolar

“Ochre (Stop)”  and   “Amber  (Stop)”   named  a^er  the   bacterial  strains  in   which  they  were   iden_fied.  AUG  is   the  most  common   ini_ator  codon;   GUG  usually  codes   for  valine,  and  CUG   for  arginine,  but,   rarely,  these   codons  can  also   code  for   methionine  to   ini_ate  an  mRNA   chain  

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Molecular components of cells  
Molecular components of cells  

Teaching Program on Molecular Pathology - Principles of Molecular Biology.