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Molecular  Pathology  

Molecular  Biology  Principles  

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Molecular  Pathology  

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Requires fluorescent ! tag for desired cell!

Molecular  Biology  Principles  

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Molecular  Pathology  

Molecular  Biology  Principles  

h/p://www.interpathology.org  

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Molecular  Pathology  

Molecular  Biology  Principles  

h/p://www.interpathology.org  

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Molecular  Pathology  

h/p://www.interpathology.org  

The two faces of a membrane are asymmetric in terms of lipid and protein composition!

Molecular  Biology  Principles  

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Molecular  Pathology  

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“The Fluid Mosaic Model”!

Mobility (diffusion) of a given membrane components depends on:!   the size of the molecule!   its interactions with other molecules!   temperature!   lipid composition (tails, cholesterol)! Mobility can be measured by “FRAP”!

Molecular  Biology  Principles  

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Molecular  Pathology  

Molecular  Biology  Principles  

h/p://www.interpathology.org  

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Molecular  Pathology  

Molecular  Biology  Principles  

h/p://www.interpathology.org  

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Molecular  Pathology  

Molecular  Biology  Principles  

h/p://www.interpathology.org  

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Molecular  Pathology  

Molecular  Biology  Principles  

h/p://www.interpathology.org  

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Molecular  Pathology  

Molecular  Biology  Principles  

h/p://www.interpathology.org  

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Molecular  Pathology  

Molecular  Biology  Principles  

h/p://www.interpathology.org  

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Molecular  Pathology  

Molecular  Biology  Principles  

h/p://www.interpathology.org  

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Molecular  Pathology  

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A, G, T, C are present in DNA! A, G, U, C are present in RNA !

Molecular  Biology  Principles  

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Molecular  Pathology  

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Hydrogen bonding between complementary base pairs (A-T or G-C) holds the two ! strands together!

Molecular  Biology  Principles  

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Molecular  Pathology  

Molecular  Biology  Principles  

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Molecular  Pathology  

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supercoiled!

Molecular  Biology  Principles  

relaxed!

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Molecular  Pathology  

Molecular  Biology  Principles  

h/p://www.interpathology.org  

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Molecular  Pathology  

Molecular  Biology  Principles  

h/p://www.interpathology.org  

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Molecular  Pathology  

Molecular  Biology  Principles  

h/p://www.interpathology.org  

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Molecular  Pathology  

Molecular  Biology  Principles  

h/p://www.interpathology.org  

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Molecular  Pathology  

Molecular  Biology  Principles  

h/p://www.interpathology.org  

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Molecular  Pathology  

Molecular  Biology  Principles  

h/p://www.interpathology.org  

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Molecular  Pathology  

h/p://www.interpathology.org  

2nd  base   U  

1st  base  –  3rd  base  in  each  row  

U  

C  

A  

G  

Molecular  Biology  Principles  

C  

Basic  

G  

Acidic  

UUU  (Phe/F)  

UCU  (Ser/S)  

UAU  (Tyr/T)  

A  

UGU  (Cys/C)  

Polar  

UUC  (Phe/F)  

UCC  (Ser/S)  

UAC  (Tyr/T)  

UGC  (Cys/C)  

UUA  (Leu/L)  

UCA  (Ser/S)  

UAA  -­‐  Ochre  (Stop)  

UGA  -­‐  Opal  (Stop)  

UUG  (Leu/L)  

UCG  (Ser/S)  

UAG  -­‐  Amber  (Stop)  

UGG  (Trp/W)  

CUU  (Leu/L)  

CCU  (Pro/P)  

CAU  (His/H)  

CGU  (Arg/R)  

CUC  (Leu/L)  

CCC  (Pro/P)  

CAC  (His/H)  

CGC  (Arg/R)  

CUA  (Leu/L)  

CCA  (Pro/P)  

CAA  (Gln/Q)  

CGA  (Arg/R)  

CUG  (Leu/L)  

CCG  (Pro/P)  

CAG  (Gln/Q)  

CGG  (Arg/R)  

AUU  (Ile/I)  

ACU  (Thr/T)  

AAU  (Asn/N)  

AGU  (Ser/S)  

AUC  (Ile/I)  

ACC  (Thr/T)  

AAC  (Asn/N)  

AGC  (Ser/S)  

AUA  (Ile/I)  

ACA  (Thr/T)  

AAA  (Lys/K)  

AGA  (Arg/R)  

AUG  (Met/M)  

ACG  (Thr/T)  

AAG  (Lys/K)  

AGG  (Arg/R)  

GUU  (Val/V)  

GCU  (Ala/A)  

GAU  (Asp/D)  

GGU  (Gly/G)  

GUC  (Val/V)  

GCC  (Ala/A)  

GAC  (Asp/D)  

GGC  (Gly/G)  

GUA  (Val/V)  

GCA  (Ala/A)  

GAA  (Glu/E)  

GGA  (Gly/G)  

GUG  (Val/V)  

GCG  (Ala/A)  

GAG  (Glu/E)  

GGG  (Gly/G)  

Nonpolar  

“Ochre  (Stop)”  and   “Amber  (Stop)”   named  a^er  the   bacterial  strains  in   which  they  were   iden_fied.  AUG  is   the  most  common   ini_ator  codon;   GUG  usually  codes   for  valine,  and  CUG   for  arginine,  but,   rarely,  these   codons  can  also   code  for   methionine  to   ini_ate  an  mRNA   chain  

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Molecular components of cells  

Teaching Program on Molecular Pathology - Principles of Molecular Biology.

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