2003 54 hasina et al

3x~n!at\CH nypnypnaH

Echinacea purpurea

(L.)

Moench

npnMeHHeTCH

JJ.ml JlClJCOHO-npocjJHJTaKTHqecKYIX UColCH B MCilHUHHCKOH npaKTHKC B Kaqense JcjJcjJeRTHBHOro 11MMYHOCTI1MY1ll!Topa

(7, 9, 12).

Ocnosy ocjJHI.111H<DlbHhiX npe-

rraparos, !II1lliCBblX 11 KOpMOBbiX JJ.06aBOK 113 JXIHI3UCI1 nypnypHOH COCTaBJlliIOT.

K3K

upaBHJlO,

ITOJIHcjJCHOJlbl

11 llOJTI1CaXap111lbl.

8

OT,lHqHe

OT

BOJJ.HO-

CIIHpTOBbiX 3KCTp3KTOB (H3CTOCK) JXHH3UCH rrypnypHOH, CO,UCp)f(3WHX npeHMYWCCTBCHHO ITOJIHcjJeHOJI.bl, 6110JI.Ofi1'ICCK3H aii."TI1BHOCTb 11 <!JapMaKOJTOfHIJCCKHC 3cpcjJeKTbl llOJIHCaxap11JJ.OB M3.JIO I13Y'ICHbl, H OO,lbllii1HCTBO HCCJICJJ.OBaHHH

nocBHII~CHbi 11x 11MMYHOCTHMynupyiOWeMy ,llelicTBHIO

(8, 11 ).

B 1111reparype

CCTb CBCJJ.CHHH 0 TOM, <frO ITOJll1C3X3p11,UHbiH KOMITJTCKC JXI1H3UeH KaK Hecneumj:li1IJCCKHH CT11MYJTl!Top noBhiWaCT pe3HCTCHTHOCTh opraHHJMa, era yCToi-1IJHBOCTb K CTpeccy, CT30HJTH311pyer ropMOHrulbHbiC cpyHKUHH, CTHMy,111pyeT CllCpM3TOfCHC3, np11 JTOM HC HMCCT ITOOOIJHOfO Jl.CHCTimH 11 OCT3BJ1HCT CCJ1hCKOX03HHCTBCHHYIO npOJlYKll1110 3KOJ10rHIJCCKI1 '111CTOH

(1 ).

11CXOM li3 MHOfO-

IJI1CJlCHHbiX )l3HHbiX H3YIJHOH Jli1TCpaTypbl 0 llOJTHBaJTCHTHOM .UCHCTBHI1 pa3.JUPfHhlX llCKTI1HOBbiX IlOJII1C3X3p11.LlOB, )"rBCp)K,LlCHHC 0 llOBblillCHHI1 a,IlarrT3U110HHbiX B03MO)!(HOCTCH CCJ1bCKOX03l!HCTBCHHblX )!(11BOTHbiX H 'ICJlOBCKa K B03)lCHCTBI110 3K0"10rHIJCCKHX cpaii."TOpOB p33J111IJHOH np11p0Jl.bl npaBOMO'lHO, HO Tpe6yer myOOKOrD OOOCHOBaHIUI Ha <fJYHKUHOHaJTbHO-MCTaOOJlliiJCCKOM ypoBHC. I..l,CJTb!O .llCikTBIDI

HaCTOHIUCH pa60Tbl liBHJlOCb BhiHBJlCHHC remrronpoTCKTI1BHOfO

BO.llOpaCTBOpHMbiX llOJII1C3XapH.llOB 3X11H3UC11

nypnypHOH,

Bblpa-

IHCHHOH B np11MOpCKOM Kpae PoccHHCKOH cDe,TJ.epaUI111, 13 ycnOBHHX JKcnep11-

xao1Ha 3. 11., crpe6Hesa M. H., XOMeHKO B. A.*, CO~OBbeBa T. $.* ~HCTHTYT 6~oror~~ Mop~ ~BC PAH.

MCirra:lbHO Bbi3B3HHOrO y KpbiC TOKCI1IJCCKOI'O renaTHT3.

Ha Kphrcax J111HHH BHCTap 113YIJaJJacb 34JcjJeKT11BHOCTb rrpHMCHCHI1H so.no-

PoccvH'I, r. BnaL(>130CTOK, yr. flanb'·BBCKoro, 17, 690041, • THXOOK03HCKM~ MHCT~TY" 6~00prBHMY€CKOR X~M~M ~BQ

PoccHR, r. BnaAHBOCTOK, np

PAH.

100-neT BnaAHBOCTOKy, 159, 690022

IEnATOnPOTEKT~BHOE ~E~CTB~E non~CAXAP~~OB 3X~HAUE~ nYPnYPHO~ MHoroi.JHCJJeHHhle

KJIHHWtecKHe

11

::JKcnepuMeHTru'1bHhie

11ccne.uosaHHJ1

pacTBop11MbiX ITOJlHCaxapH,Il,OB C JTCIJCOHO-rrpocp111laKTH'ICCKOH l.IC.Tb!O Ha MOJl.C-

~111 TOKCHIJecKoro rerranrra, Bhi3BaHHoro rerpaxnopMeTaHoM. TpH rpyiTilhl ITO TpOJib ), rpeThH

7 :lKHBOTHhiX: nepBaJI- "11HT3.KHJall 11 , BTOpaH - "rerrar11r + nonucaxapw.ubr". Co.uep)f(aHI1e

n onhrre

6hmo

"renann" (KOH11 JBTaH3311H )f(H-

BOTHbiX npoBO)lHJlaCh B COOTBCTCTBI1H C XeHbCHHCKOH .UeKJ1apai111CH IlO pa6oTe c na6opa:ropHhiMH )!(J1BOTHhiMI1. TerpaxnopMCTaB BBO.lli111H 30H.IIOM B )KCllY.llDK,

CBHJJ.CTCJlhCTBYIOT 06 3cpljJcKTI1BBOCTH llOJTHCaxapH,IIOB paCTI1TCJlbHOrO H )I(H-

C)f(C~HCBHO, O.llHOKpaTHO, B TC'-lCHHC

BOTHOrO np011CXO)K)lCHI1H (aJTbrHBOB3JI KHCJIOTa, XI1T033H, UHTpyCOBbiH, HO-

pacTBopa B OJ111BKOBOM Mac"1e B ,uo1e

14 CyY. 3a '-laC .llO CJJ_bl B BH.llC 10 %-HOfO 300 MriKr. Bo.nopa(.,'TBOpHMbie nonl1caxa-

JIO'-lHbiH ITCKTI1Hhl H Jlp.) B JIC'ICHHH 11 npocpH113Kl'11KC pa311H'-lHhlX 3360J1CB3HHH

pHJlhi BhiJl.CJlCHbl 113 H3)l3CMHOH M3CCbl pacTCHHH (1111CThH, CTC6JTH, COUBCTH)!)

'{CJ10BCJ<a H )!(11BOTHbiX. DI11I.(CBhiC Jl06aBKH Ha OCHOBC IICKTHHOBh!X TIOJ1HCaxa-

BTOporo rDJl.3 BereTaU1111 B <fla3y llBCTeHHH, B na6opaTOpH11 MOJ1CKY11HpHbiX DC-

p11JlOB W11pOKO HCil0Jlb3YIOTOl np11 11HTOKCHK3UI111 opraHM3M3 Ta:)f(CJlbiMH MC-

HOB 3HTH6aKTCpHaJTbHOro 11MMYHI1TCT3 TI1XOOKCaHCKOrD HHCTI1TyYa 6noopra-

T3.JUI3MI1, pa,UHOHYKJHI.UaMH, ITCCTHil!iJl.aMH, KlUIJeqBhiX 11HcpeKUHHX, MCliMKa-

HH'-lCCKOi1 xwMI111 ):tBO PAH. 3KCTpaKUHIO ocyiHCCTB.1HJ111 so.noM. c

MCHT03HOM 110p3)f(CHHI1 )!(CJlYliKa, Bl1PYCHOM 11 TOKCWICCKOM renantrc, rYJnep-

.uyrou.(HM OC3)!(Jl.CHHCM

JlHTIJ1.llCMHHX 11 ;:ma6ere, HMMYHO,TICcpHUlfTHblX COCTOHHI1HX pa3S1WHIOH :HHOJlO-

HOfl paHee

ApceH~Ll IIHU.\CBbiX .uo6asoK Ha ocHose rreKTHHOBbiX nonHcaxap11-

)!(11BOTHbiC

nm

(5, 13 ).

noc.rre-

96 %-HbiM JTHJlOBbiM Clll1pYOM Ha X0.10)lC IIO OITI1CaHMCTO.llHKe (3). Br,Ixo.n coCTaBHJ1 8,2 %. DpenapaT nomtcaxapw.uos nonyqanu B Bl1.llC 1 o/o rem! B .ll03C J 00 Mr/Kr B TCIJCIIHC 2-X HC.llCJlb

1lOB llOCTOHHHO llOITOJIHHCTCH KaK 33 Ct!CT XOpOlUO H3BCCTHbiX J1CKapLTBCHiibiX

Ja 'lac no sae.ueHHH rcrpa:mopMeraHa, BHYTPI1)Ke,TyllDIJHO, OJJ.HH pa3 B llCHb.

pacTCHHH, T3K 11 MaJIOI13Y'ICHHOH cjJ:IOpb!.

YKa1aHnLre s ra6mme 6woxHM11IJecKHe noKaJarenH, xapaKTCPH3Y!OlU11C Mera-

212

Ex libris Травы жизни: http://medherbarium.blogspot.com

60JJW1CCKOC H ljJyHKUHOH<UihHOC COCTO:i!IHIC neqeJIH, onpCJleJlllJIH 06LUellpHH!'ITI10HpCJIYKTaJbl H Ll10Tll.THOHnepOKCH)lll.3bl COXp3W!Jill.Cb Ha 6onee Bb!COKOM

Tbl:>iH B KJIHHHKC H :n<cnepHMCHTaJlhHOH TepaHHH MCTOllaMH.

Te-rpaxnopMeraH

ypOBHe ( 81 H

('1eTbipexx:10piiCTblti ymcpo:1) llB2llleTCH rcnaToTponHb!M

llllOM 11 lUHpOKO Tip11MCHll:efC51 K3K TOKCI1K3HT, C lJOMOLUhKl KOTOporo lJ )KCf1Cp11MCHT3c'IhHOH TeparmM ~!O;lCJmpyerCH OCTph!H H XpOHH'ICCKHii renaTHThl.

83 %),

4eM B KOIITpOJibHOH rpynne ( 44 H

6) % ).

Pe3yJibTaThi nacTOlllllCH pa6oThl noKa3:uH, •rro BO)lopaCTBopnMhie

rto-

nmmMe-

caxapHLihi JXI1HaUel1 nypnypHOH OKa3MBaiQT llOJIOJKllTCJlbHOe BJIIUIHHC Ha

;lYl.JCHHbJC D HaCTO.I!ll{CH pa6oTe HapyWClHUI MCT360JJWICCKOfO 11 <)>yHKUMOHaJ!h·

Tll.60JlH'1CCKHe ripOLI,CCCbl 11 $yHKl(HOHa.JibHOC COCTORHHC fle'{CHH np>l TOKCI1t.IC-

HOfO COCTOllHI1:il ner!CHH DUOJJHC COfJiaCyiOTCll: C KJiaCCif'fCCKMM 11pCJ1'-'T3BJICHHCM

CKOM renanne, a HMCHHO

naToreHeTI1'1CCKHX .'.fexaHH3MOB nel1:cmH>J ua Hee T<.'TpaxnopMeraHa 1tepe3

2

KHX renaTOCIICU11<jlH'1CCK¥fX <jlepMCHTOH-M3pKepOB, K3K a.JJafHJHai\HIHOTpa.::<jle!l ypOKa!Hnta3a B

2,4

3

H

pa3a COOTBeTCTBeHHO (CM. TaOnll!J.y). lfOBbUUC-

2,6

HHe CO.ll,Cp:lKaHHS! OHilHpy6HHa B Ch!BOpOTKC KpOBH (B

pa3a B cpaBHCHHM C

nop!'dol1:) YKa3bmaeT Ha HapyurcHMc 3l\CKpeTOpHoi1 <)>ynKIJ.HH

ne•rem1.

OnHo-

speMeHHO C JTifM B llC4CHH OTMC'{3CTCll: 3KTI1BatUI)I HpOUCCCOB JH1TlOfCHC1a H rrepeKI1CHOl'O OKHCJJCHHH IlHminO!~: B CbiBOpOTKC KpOBI1 ypOBCllh OOllJ.CfO XOSICCTCpHHa 11 TpHn111UCp11)l08 6hlJI BbllliC HOpMbl Ha

53

11

93 %

COOTBCTCTIJCHHO,

B ne4eHH con.cptKaHHe o6u(HX ,111lll1llOB npeabrma.no HHTaKTHhli1 tpon Ha

06

m-

R:numue nOJmcaxapnJloB 'JXHHaueu nypnypl:loif ua pu.u 6noxHMH<JeCKHX nm.:a.~. ~~~T~!~il _I:I·IIJQPQTKH_Kpo~.l!.!' !!e_'l~lf.f!!I.PH TI}_K.E.J!~~~(J'!f_r:.f:naTHTe (!\-!__:!:_!Ill.__ ! l~---~~~ ~£'£X!11Ihl.2!'ltBOTHhlX . . .. i

HC,!:U~JJH HHCllCHlfll: TOKCHKaHTa B IIC'lCHI1 Bbipa)I{CHbl U,MTOIIHTH'1C-

CKI1C npOUCCCbl, 0 '{CM CBI1l1efCJibCTl3)'CT YBCJIH'ICHHC B CbiBOpOTKC KpOBH Ta-

pa:Ja

npe111!TCTBYIOT UHTOJlHTHqecKOMy npoueccy,

nepJIHl1l1llCMHI1, IIHIIOnepOKCHllaU}fH, HapyUJeHHIO CC 3KCKperopHOH <jlyHKUHH.

(6).

a

49 %.

ycH~lCHI1H nepOKCli)laiJ.HH JlHIIH)lOB H liO.!l.aBJICHI1H aKHTMOKCH,.claHTHOif 33-

IL . l1oKa:mTem1 .I HHT3KTH3ll i "renaT~KOH_:-r.;~;:;a.~:;;;:-; no--i [_- --' cl:iiopo·~~atiP~;~--11'~~1-. LnHcax_al'.H!•l>!"i pi~I<a~;~~~~l.M.."~~~J~·~~---C~~~~,;:t: o=3I~~T. .•-.Jj,[~LT~*=~Lio~ ± o:S.~~~j IAJtaHI1HaMHHOTjJaUC4Jepa:sa, r i i

I

f

I

i

MMOJlh/JJ·q.

0,80±0,05

.

r -- 661~ijfifiMJJ;;py6HH,

l

t-

,

1,90±0,17*

},42±0,12**!

..... -T

_____ r,j~MOJ!b~JJ__

9,7 ±. 0,72

25,8 ± 2,4*

lliliTbl B DC'{CIUI fOUOp11T Bb!COKHH ypOBCHb MaJJOHOBOfO .D.HaJlh)lETH)la B CbiBOpOTKC KpOmi H CHlfii<CIUlC aKTHBHOCTH fiiHJTaTHOHpCJlYKT33bl 11 fJIKlTaTI10HilCpOKC11.L(a3bl B ITC'iCHH ( 208,

B )'CJIOBIHIX

44

11

61 %

OTHOCIITCJibHO HOpMbl ).

HHTOKCMKai.(Inl Kph!C TCTpax.rropMeTaHOM IIOJllfCaxapliiU>l :>XH-

JmUC!i rrypnypHOH npeJwnpeJK)la.JIH HapyrneHHll tPYHKUI1H 11 MCTa6o;unMa B !lC'iCHH, I1p0HBH.I!ll BhlpaJKCHHYJ<l renaT03aJ1UlTHYIO aKTHBHOCTb. noJlHCaxapH)lf,! npcml.TCTBOBll.HH pa3BI1THIO lliiTOJIH3a: 3KTHBHOCTb ypOKaHI1Hll.3hl 11 a.JI31HI-

rJJIOTaTHOHpe.llyKTa:'!a,

Fl TO BpeM~I Kll.K B

_____ liM:().fi_I,/~tr 6eJI_~a~r.l1i_".·

HaMHI10Tpa!:iC{pepa3bl OL'JH'laJJaCb OT HOpMbl Ha KOHTpOJJC - Ha

204

If

137 %

I 03

H

77 %,

COOTBCTCTBeHHO. npenapaT IIOJUfCaXap11,\0B CliO-

C06CTBOBa.JI coxpaHemno 3KCr<peropHOH <jlyHKL!HH ne4CHH, OCJJa6JUill fH11Cp6Hmrpy6HHCMH!O: pOCT KOJ1U4CCTLla 6HHHpyOI1Hll. B Cb!BOpOTKC KpOilH )lOCTHf

%

(B rpynnc:: "renaTI1T''-

164 %).

74

noHHC3X3pH)lbl 3XI1H3UeH llpOHIBO.Jei:JcTBO-

FlaJHl rHrrepmmH.UeMHM, Bbl3brBaeMoi1 TeTpaxllopr.teTaHoM.

B

CbiDOpOTKC Kpomt

con.eplKaHHe o6wero xoJleCTepnna n TpHrllHilCp11JlOB non BJJH~HHCM npenapaTa YBCJIH'UfBa.JIOCh TOJlbKO Ha

8

H

43 %

(B KOIITpOJle

53

H

93 %),

JIH4CCTB0 OOIIlHX J1!1lllf,1](1B OTHOCIITCJlbHO HOpMLI COCTaBJUIJIO BpeMli KaK B rpynnc, HC noJJy'iaBLUCH lTOJlHCaxapHD.,

149 %.

:

L'IIOTaTI10HnepoKcH/l33_a_, ~~-···~·-·~-----if-·~---~------+·~---·------

1

___!i~O]J~/111 !:.. ~!:J!Ka · MHH ·----"--..--

* p < 0,05 npu cpmmenuu zpynn npu cpaeue;wu zpynn "zenamum" u "c'enamum + noJlucaxapur)bl ".

B Hay•moi1 J111Teparype yme llOl\BHJJOCb 6mibUlOC qwcno pa6oT

o MHOrHX

B33HMOCBll33HHbiX H o6ycnaBJlHBalOil(HX .D.pyr )lpyra <)>apMaKOJlOfff'.JeCKHX 3<jl-

B fiC'iCHH KO·

QJeKTax liCKTI1HOBblX IIOJIHCaxapHJ'l:OB 113 paCTCHHH paJJIH'IHbiX CeMeHCTB: Kpo-

120 %,

Me HMMYiiOCTHMYJIHPYIDUiero, o6 aHTHOKCH)laHTHOM, rmwnHHHllCMH4ecKoM.

13 TO

AHT110KCH)l£lHf-

renaTonpoTeKIHBHOM, :mTepocop6UHOHHOM H .D.p. ( 4,

I 0).

3<)><jleKTJIBHOCTb

no~

Hall: aKTHBHOCTb npe11apara rip011Bllf!aCb B TOpMO)I{CHHH pa3BHTI1.!! npouecca

JIHCUXapH.UOB B JICqCHHH H npo<jJHJJaKHIKC promi'IHb!X 3300JICB3HUH O)lHlf aB-

nepOKClillll.UH.H mHJH)lOB: ypoDeHb Ma.i!OHOBOfO )lHilllh)lCI'H)lll. B ero llpHCyrCT·

TOphi CBll3blBll.IOT C JHTepocop6UJ-ICH B )I{CJlY)lO':IHO-KHllieqHOM TpaKTe 3H)lO- H

BHH flOBbllllaJJC)I Ha

32 %

a 13 KOHTpOJle

Ha

108 %.

nonMcaxapH.!l.hl npertliTCT-

BOB<lJIH HCTOIUCHHIO ll.HTHOKCHllll.HTHOH CI1CTCMbl liC'Iemr: ll.KTHBIIOCTb L'JIOT3·

JK30TOKClfHOB 11 HX BbiBC.lleHHeM, .D.pyme TCMY opraHHJMa, TPCThH

C BJlHliHMCM Ha 3H)J,OKplilfHYIO CHC-

TOJlbKO c 11MMYHOMO.!l.Y1111pyronl.UM 3QJ<)>eKTOM H

L ,J. <l>apMUKOKHHCTHKa O,!lHOrO l13 flCKTHHOBhfX llOJIHCaXapM,ilOB

30CTCpHIIa,

Bhl.ile;JeHHOfO H3 MOpCKOH TpaBbl Zostera asiatica, CBH[(CTC;JbCTBYCT 0 H3KOII-

215

Ex libris Травы жизни: http://medherbarium.blogspot.com

20 % ~cTKH (H-"JocTepHH) so BHYTPCHHHX oprarmx, •no upe,Jno.laraeT HenocpencTBeHHoe em BmumHe Ha KncTOY:HOM ypoBHe (2). MexaHH3'1 neikTBIUl (\10)KeT 6h1Th, !ICCKO!lbKHX ~CX3.HI13~0B) 'JTOH rpym1hi 6HOclOTTI'ICCKH aKTHBHhlX BC!IlCCTB liOKa HC SlCCH, erO npCllCTOHT BbUICHHTb B 6ynymeM. JlCHHII

JiHO.lHOrpal)lHH

1. MHpOHeHKO E. 11. 11cnom>'30B3Hl1e Jxl1HaHCl1 nyprrypJIOi1 B )KHBOTHOBOilCTBe // ~13)'lJCHl1C l1 HCilOJTh30B3HHC 'JXI1H3UCH. Ma:rep. MC)KJ.!yHapOJl. H3y4. KOH!flepeHI!. fiomasa, 21 24 ceHT)I6p5! 1998 r. fimiTaBa: BepcTKa, 1998. C. 138

12. Melchart D.. Linde K., Worku F. et al. Tmmunomodulation with Echinacea a systematic review of controlled clinical trials // Phytomedicine. 1994.

Vol. 1. - P. 245- 254.

13. Thakur B. R., Singh R. K., Handa A. K. Chemi5try and Uses of Pectin A Review// Crit. Rev. Food Sci. Nutr. 1997.- Vol. 37 .. No l.- P. 47- 73.

- 140.

2. rionoB A. M., J1RMKHHa

r. n., ApTroKOB A. A. I! Jt:p. l13y<IeHHe !flapM3KOKHHCTI1Kl1 30CTepHHa 11CKTI1H3 I13 MOpCKOH TpaBbl Zostera asiatica // }X.t\H CCCP. 1990.- T. 315.- N2 I.-- C. 232-235.

3. Co.:rom.esa T. <I>., EeceD.HOBa H. H., Ysaposa H. 11. 11 )Jp. ct>arot{HT03CTHMY,lnpyf0mee nei:fcTBHC fiOJIHCaxapvl.llOB, Bhl,llCJ!eHHh!X H3 KYJTbTypb1 TKalleH )KCHhiJICH>I // AHTH6HOTI1KH 11 XHMHOTepamDL 1989. T. 34. N~ 10. C. 755-760.

4. XaClma 3. H., Koi!eHl.feHKO E. A., Crpe6HeBa M. H. H Jlp. AirrnoKcnrt:aHTHall aKTHBHOCTh HmKmrcpn!fJHQHposaHHoro neKnma 113 MopcKOH TpaBbl Zostera marina// B11onontll MOpSl. 2003. }[,. 5. C. 120 - 125.

5. XoTHM4'CHKO IO. C., KponoTCB A. B., XoTr1MLJCHKO M. K). (!JapMaKoJro:-wieCKHe CBOHCTBa neKT!1HOB !I ~.:J!fl(jJepeHrHaSJ TeparmH. -- 200 I.- T. 7. N~ 4. C. 22 36.

6. lleThlpexX,10p!iCTb!H yr.:repO.!l I no)J. pe;r. H. <:D. Yi3Mepoaa. - M.: UMfl fKtU, 1983. - 20 c.

7. Bauer R., Wagner R Echinacea species as potencial immunostilatory drugs// Economic and Medical Plant Research I Ed. H. Wagner. London: Academic Press, 1991. 150 p.

8. Emmendoerfer A. C., Wagner H., Lohrnann-Matthes M. L. Immunological active polysaccharides from Echinacea purpurea plant and cell cultures // lrnmunomodulatory Agents from Plants I Ed. H. Wagner. Base!: Birkhauser, 1999.

P. 89-124.

9. FosterS. Echinacea Nature's Immune Enhancer. Vermont: Rochester, 1991.

-- 150 p.

10. Hase K., Basnet P., Kadoto S., Namba T. Immunostimulating activity of Celosian, an antihepatotoxic polysaccharide isolated from Celosia argentea // Planta Med. 1997.- Vol. 63. P. 216 ... 219.

11. Melchart D., Clemm Ch., Weber B. et al. Polysaccharides Isolated from Echinacea purpurea herba Cell Cultures to Coumract Undesired Effect of Chemotherapy-· a Pilot Study// Phytotherapy Res. 2002. Vol. 16. -X£ I. -· P. 138- 142.

16

217

Ex libris Травы жизни: http://medherbarium.blogspot.com