Test Bank for Burtons Microbiology for the Health Sciences 9 North Americanth
Edition Paul G Engelkirk
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1. It would be necessary to use a tuberculocidal agent to kill a particular species of:
A) Clostridium.
B) Mycobacterium.
C) Staphylococcus.
D) Streptococcus.
2. Pasteurization is an example of what kind of technique?
A) antiseptic
B) disinfection
C) sterilization
D) surgical aseptic
3. The combination of freezing and drying is known as:
A) desiccation.
B) lyophilization.
C) pasteurization.
D) tyndallization.
4. Organisms that live in and around hydrothermal vents at the bottom of the ocean are:
A) acidophilic, psychrophilic, and halophilic.
B) halophilic, alkaliphilic, and psychrophilic.
C) halophilic, psychrophilic, and barophilic.
D) halophilic, thermophilic, and barophilic.
5. When placed into a hypertonic solution, a bacterial cell will:
A) take in more water than it releases.
B) lyse.
C) shrink.
D) swell.
6. To prevent Clostridium infections in a hospital setting, what kind of disinfectant should be used?
A) fungicidal
B) pseudomonicidal
C) sporicidal
D) tuberculocidal
7. Sterilization can be accomplished by use of:
A) an autoclave.
B) antiseptics.
C) medical aseptic techniques.
D) pasteurization.
8. The goal of medical asepsis is to kill ____________________, whereas the goal of surgical asepsis is to kill ____________________.
A) all microorganisms …pathogens
B) bacteria…bacteria and viruses
C) nonpathogens…pathogens
D) pathogens…all microorganisms
9. Which one of the following types of culture media is selective and differential?
A) blood agar
B) MacConkey agar
C) phenylethyl alcohol agar
D) Thayer-Martin agar
10. All of the following types of culture media are enriched and selective except:
A) blood agar.
B) colistin-nalidixic acid agar.
C) phenylethyl alcohol agar
D) Thayer-Martin agar.
Use the following to answer questions 11-15: Match the following items with the correct phrases.
a. capnophiles
b. fastidious organisms
c. halophiles
d. microaerophiles
e. psychrotrophs
11. Organisms that prefer salty environments are called ____________________.
12. Organisms that have especially demanding nutritional requirements are called ____________________.
13. Organisms that prefer environments having decreased concentrations of oxygen are called ____________________.
14. Organisms that prefer refrigerator temperature are called ____________________.
15. Organisms that grow best in environments containing increased concentrations of carbon dioxide are called _______________.
Use the following to answer questions 16-20: Match the following items with the correct phrases.
a. blood agar
b. chocolate agar
c. MacConkey agar
d. phenylethyl alcohol agar
e. Thayer-Martin agar
16. Although Neisseria gonorrhoeae and Haemophilus influenzae will not grow on blood agar, they will grow on ____________________.
17. ____________________ is a highly enriched and highly selective medium, used to isolate Neisseria gonorrhoeae from clinical specimens.
18. ____________________ is an example of a medium that is both enriched and differential.
19. ____________________ is selective for Gram-positive bacteria.
20. ____________________ is an example of a medium that is selective and differential.
21. A bacterial cell would swell and burst if placed in an extremely hypertonic solution.
A) True
B) False
22. Pasteurization is a method of sterilizing liquids.
A) True B) False
23. Archaeans that live in or near hydrothermal vents at the bottom of the ocean are halophilic, barophilic, and thermophilic.
A) True
B) False
24. Lyophilization is an excellent method of killing microorganisms.
A) True B) False
25. Antiseptic technique is a type of aseptic technique.
A) True B) False
26. In an autoclave, microorganisms are killed by ethylene oxide.
A) True
B) False
27. The goal of disinfection is to kill all microorganisms.
A) True
B) False
28. It is not possible to culture protozoa in the laboratory.
A) True B) False
29. Rapid freezing, using liquid nitrogen, is an excellent method of killing microorganisms.
A) True
B) False
30. Microorganisms can be killed by direct exposure to ultraviolet light.
A) True B) False
Use the following to answer questions 31-34:
Match the following items with the correct phrases.
a. enriched and differential
b. enriched and selective
c. selective and differential
d. selective only
e. differential only
31. Phenylethyl alcohol agar and Thayer-Martin agar.
32. MacConkey agar and mannitol salt agar.
33. Sabouraud dextrose agar.
34. Blood agar.
Use the following to answer questions 35-41:
Match the following items with the correct phrases.
a disinfection
b. pasteurization
c. sanitization
d. sterilization
35. The complete destruction of all living organisms, including cells, spores, and viruses.
36. The use of antiseptics is an example of a __________ technique.
37. Autoclaving for 15 to 20 minutes at 15 psi and 121°C is one method of accomplishing this.
38. The destruction or removal of pathogens by physical or chemical methods.
39. Heating to 60°C for 30 minutes is one method of accomplishing this; sufficient to kill pathogens and reduce total bacterial counts.
40. Heating in a 160°C oven for 2 hours would accomplish this.
41. The reduction of microbial populations to levels considered safe by public health standards.
Use the following to answer questions 42-47: Match the following items with the correct phrases.
a. mesophiles
b. thermophiles
c. psychrophiles
d. psychrotrophs
e. halophiles
42. The brightly colored algae that grow in the hot springs at Yellowstone National Park are examples.
43. Organisms in this category could grow on mannitol-salt-agar.
44. Most human pathogens are in this category.
45. Bacteria that live in the ocean are examples, regardless of the temperature of the water.
46. A specific group of microorganisms whose optimal growth temperature is 4°C.
47. Vibrio cholerae is an example.
Use the following to answer questions 48-50:
Match the following items with the correct phrases.
a. hypertonic
b. hypotonic
c. isotonic
d. gin and tonic
48. Crenation of red blood cells would occur if they were placed into a(n) __________ solution.
49. Adding large quantities of salt or sugar to various types of foods is a way of preserving them. If bacteria should enter such foods, they would find themselves in a(n) __________ environment.
50. If red blood cells were placed into a(n) __________ solution, they might hemolyze.