Genetic Health Analysis for Dogs

Analysis

3000

DISCOVER THE SECRETS OF YOUR PATIENT’S DNA.

For Veterinarian Use Only

WHAT IS DNA? One of the keys to understanding a dog is to understand its genetics.

That starts with its DNA. What is DNA? DNA is the blue-print for life. DNA acts as the long term storage of information in the cell, the hard drive so to speak.

So, given that over 99% of the DNA in a dog is the same across all of the breeds 1 from a 2 pound Chihuahua to a 200 pound Great Dane, how can you get such extreme differences in the breeds? It depends on small alterations in the DNA from breed to breed.

Even within a breed, there can be variations.

When each dog is conceived, he receives two copies of DNA, one from the father and one from the mother. Which pieces of DNA are inherited from each parent is completely random. Most variations come in the form of SNPs, or single nucleotide polymorphisms.

Polymorphism

A C T C A G T T G A General population

A C T C A G T T T A Single nucleotide polymorphism (SNP)

Chondrodysplasia in dogs: FGF4 retrogene 2 Chondrodysplasia is a semi-dominant trait that causes early closure of the growth plates in dogs. Chondrodysplastic breeds like the Corgi, Dachshund, and Basset Hound have two copies of this mutation, so that they do not grow very tall. Crosses of these breeds with non-chondrodysplastic dogs results in puppies with only one copy of this mutation so their growth plates don’t close quite as early resulting in slightly longer and less twisted legs. This can sometimes be seen in mixed breeds like the Beagle-Basset Hound mix.

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DNA AND PET CARE As a veterinarian, understanding a dog’s genetic makeup can help you talk to pet owners about why their dog may have an appearance, behavior, or health care need that is different from other dogs.

DNA testing can reveal the dog’s breed mix and with the help of science-based evidence, you can answer health-related questions more accurately and help the pet owner to care for their dog properly.

Use DNA testing as a tool to help you develop a custom care and wellness plan for your patient.

Identify breed mix and specific needs for overall health maintenance

Know a more accurate adult weight range for a more precise nutritional recommendation Understand genetic markers that could signal a predisposition to certain health conditions Build a stronger relationship with your client by knowing the pet’s health blueprint

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WHAT IS GENETIC HEALTH ANALYSIS? With a Royal Canin Genetic Health AnalysisTM kit, you and your veterinarian will: •

Identify genetic mutations that could cause health issues in the future.

Discover the breeds that make up your dog, all the way back to great-grandparents.

•

Develop a customized health and nutrition plan based on your dog’s DNA .

•

RoyalCaninGHA.com

© ROYAL CANIN SAS 2014. All Rights Reserved.

Product must be submitted within one year of purchase.

Analysis

DEVELOPED IN PARTNERSHIP WITH GENOSCOPER LABORATORIES

Analysis

Analysis

YOUR DOG’S PAST CAN TELL YOU

A LOT ™ ABOUT ITS FUTURE. The ROYAL CANIN® Genetic Health Analysis leverages cutting edge science to examine the unique genetic sequence of a dog.

The analysis uses the dog’s DNA to help identify future health needs. Based on the results, veterinarians can work with their clients to develop a customized health and wellness plan for the dog.

3000

3 EASY STEPS TO DISCOVER YOUR DOG’S DNA.

1 2 3

VETERINARIAN TAKES SAMPLE

VETERINARIAN ACTIVATION & MAILING

VETERINARIAN CONSULTATION

Highlighted features include: The only blood-based breed identification test on the market, and the only one that is exclusively sold by veterinary practices.

Analyzes over 3,000 genetic markers to identify matches to unique breed signatures that cover more than 250 breeds, types, and varieties.

Sources a database of nearly 10,000 purebred dogs which represent all of the AKC registered breeds.

Utilizes a proprietary computer algorithm that enables detection of the breeds from the parent to the great-grandparent level.

Scientific Development The ROYAL CANIN® Genetic Health Analysis™ was developed in partnership with Genoscoper Laboratories and the WALTHAM® Centre for Pet Nutrition. For more than 50 years WALTHAM has been a leading scientific authority in pet nutrition and wellness, with over 1,800 veterinary publications and a dedicated staff of renowned veterinary and nutritional scientists.

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HOW CAN GENETIC HEALTH ANALYSIS HELP? ROYAL CANIN® Genetic Health Analysis™ is designed to give you a more complete health picture of a dog, including: • Physical traits • Behavioral traits • Individual nutritional needs

Moreover, Genetic Health Analysis tests a dog’s predisposition to certain health conditions. This allows you to create a customized health and wellness plan including: • Advising clients to avoid certain medications or take other preventative measures • Monitoring for early signs of a disease • If necessary, scheduling further diagnostic testing for the disease

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HOW DOES GENETIC HEALTH ANALYSIS WORK? Although the technology behind DNA testing is complex, the process is simple.

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Take a small blood sample from the dog and submit it to the laboratory. Activate the kit online and mail to our laboratory.

Laboratory analyzes the blood sample and sends a custom report to the dog owner and veterinarian based on the findings.

As the veterinarian, you will receive a more detailed report 5 days before your client.

This is to give you time to review the report and prepare for a follow-up conversation regarding the health and wellness of the pet.

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Schedule a consultation with the dog owner to create a custom health and wellness plan based on the findings

The Genetic Health Analysis will generate two reports: one for the pet owner and one for the veterinarian. Both reports have a nutritional page that gives general guidelines based on the dog’s size, life stage, and breed makeup. A sample is shown below: NUTRITIONAL CONSIDERATIONS

NUTRITIONAL CONSIDERATIONS

ROYAL CANIN® has spent over 40 years researching the science of pet nutrition. And now, with the wealth of information from the Genetic Health Analysis™, we’re able to use our expertise to provide you with precise nutritional recommendations based on your dog’s genetics.

Optimal weight, size category and life stages

Adult weight: 42 - 47 lbs Size Category: Medium

Age until Adulthood:

Age until Seniority:

< 12 months

< 7 years

Lucy’s Nutritional Needs As an Adult: Many medium-sized dogs have sporting breeds in their ancestry that were bred to be active. The number of calories within their daily diet should be adapted to their exercise level in order to maintain a healthy weight.

Nutritional recommendation based on:

Your medium dog may put a lot of stress on his joints while at play. A diet with glucosamine, chondroitin and rich in omega-3 fatty acids can improve his joint health.

In addition to size and life stage, Genetic Health Analysis™ also identifies breeds within your dog’s family tree. Understanding nutritional needs within the breed makeup could help you and your veterinarian gain insight into selecting the optimal diet for your pet’s overall wellness.

Your dog has Labrador Retriever in its breed history. If your dog has traits that are similar to this breed, here are some nutritional factors to consider: Support healthy digestion with a diet that contains highly digestible proteins, a blend of prebiotic • fibers, and high quality carbohydrates

Life stage

•

Support a healthy skin and coat with a diet that includes EPA, DHA, and omega-6 fatty acids

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Help support healthy joints by selecting a diet with omega-3 fatty acids, glucosamine and chondroitin

Your dog has Miniature Poodle in its breed history. If your dog has traits that are similar to this breed, here are some nutritional factors to consider: Maintain dental health with kibbles that are ideal for delicate teeth. Consider kibbles that are small in • size and that create a brushing effect during chewing

Breed

•

Help maintain a healthy weight through a balanced diet that contains the optimal amount of calories to fit the dog's lifestyle and activity needs

Please remember that the nutritional considerations in this report are only a guide. Every dog is unique and has nutritional needs based on multiple factors including medical history, environment, lifestyle, and life stage. It is very important that you consult your veterinarian for a precise diet recommendation.

LUCY

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VETERINARY EXCLUSIVE BENEFITS As the veterinarian, you will receive a more detailed report 5 days before your client. This is to give you time to review the report and prepare for a follow-up conversation regarding the health and wellness of the pet. Unlike the pet owner’s report, the veterinarian’s report will contain more detailed information about the pet’s health. It will discuss health conditions that the dog may be pre-disposed to based on its individualized genetic makeup. It will also include a wellness plan that veterinarians can use for follow-up consultation. Sample pages are shown below.

ANALYSIS SUMMARY ANALYSIS SUMMARY - PAGE 9

Genetic mutation tests performed and results

Genetic mutation tests performed for Lucy: ROYAL CANIN® Genetic Health Analysis™ currently looks for more than 140 specific disease causing mutations in every sample submitted. The most common disease mutation test results as well as any others with positive results are listed below. All positive results are highlighted and additional information for those are provided below.

DISEASE Myotonia Congenita Factor VII Deficiency Hyperuricosuria Cystinuria Phosphofructokinase Deficiency

GENE CLCN1 FVII SLC2A9 SLC3A1 PFKM

Primary Lens Luxation

ADAMTS17

Pyruvate Kinase Deficiency Neonatal Encephalopathy with Seizures (NEWS)

PKLR

MODE OF INHERITANCE recessive recessive recessive recessive recessive likely recessive but may also affect some heterozygotes recessive

ATF2

recessive

TEST RESULTS negative positive - one copy positive - two copies failed to obtain result negative

Additional information based on positive test results

positive - one copy negative negative

X-linked Myotubular Myopathy

MTM1

Mucopolysaccharidosis IIIB Greyhound Polyneuropathy Pyruvate Dehydrogenase Phosphatase I Deficiency Congenital Hypothyroidism with Goiter L-2-hydroxyglutaric Aciduria Keratoconjunctivitis Sicca and Ichthyosiform Dermatitis Degenerative Myelopathy

NAGLU NDRG1

recessive in females though males only need to inherit one copy to be at risk recessive recessive

PDP1

recessive

negative

TPO

recessive

negative

positive - two copies negative negative

L2HGA

recessive

negative

FAM83H

recessive

negative

SOD1

Recessive with incomplete penetrance

negative

Factor VII Deficiency: Factor VII (FVII) deficiency is an inherited disorder in dogs that can cause mild to moderate bleeding tendencies. FVII is a vitamin K-dependent clotting factor that, when activated, plays a significant role in the initiation of coagulation. FVII deficiency is a recessive disorder meaning that dogs must inherit two copies of the mutated FVII gene to be at risk of developing the clinical signs.

Mixed-breed dogs that have inherited two copies of this mutation can also develop FVII deficiency. Educating clients with dogs who are susceptible to FVII deficiency is important so they can recognize the clinical signs and care for their dogs appropriately.

EXAMINATION REPORT

Additional information on factor VII deficiency can be seen online at: marsveterinary.force.com/royalcaningha/ Next Steps: Tests indicate that Lucy has inherited one copy of the factor VII deficiency mutation. As this is a recessive disorder, Lucy should not suffer from it and no further action is typically necessary.

Patient information Patient name: Age: Breed: Weight:

EXAMINATION REPORT

Physical exam results

Patient information

Pulse:

Temperature:

Respiration: Nutrition:

Genetic Health Analysis results and recommendations Nutrition:

Physical exam results

Feeding guidelines: Target weight: Next Visit:

GHA results and recommendations

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WHY USE GENETIC HEALTH ANALYSIS? Using ROYAL CANIN® Genetic Health Analysis™ as a tool for conversation about the pet’s health can: • Be an incremental tool to serve more clients • Serve as a gateway to additional diagnostics and nutritional recommendations • Create a health and wellness plan which includes the yearly visits that every dog needs • Help pet owners provide better care for their pets through deeper understanding • Deepen the relationship between the veterinarian and the pet owner

PRODUCT INFORMATION •

Identify genetic mutations that could cause health issues in the future.

Discover the breeds that make up your dog, all the way back to great-grandparents.

•

Develop a customized health and nutrition plan based on your dog’s DNA .

•

Analysis

RoyalCaninGHA.com

© ROYAL CANIN SAS 2014. All Rights Reserved.

Product must be submitted within one year of purchase.

Analysis

DEVELOPED IN PARTNERSHIP WITH GENOSCOPER LABORATORIES

3000

3 EASY STEPS TO DISCOVER YOUR DOG’S DNA.

1 2 3

VETERINARIAN TAKES SAMPLE

VETERINARIAN ACTIVATION & MAILING

VETERINARIAN CONSULTATION

Analysis

YOUR DOG’S PAST CAN TELL YOU A LOT ABOUT ITS FUTURE. With a Royal Canin Genetic Health AnalysisTM kit, you and your veterinarian will:

CANINE GENETIC HEALTH ANALYSIS KIT ITEM # 111113

REFERENCES 1. Kerstin Lindblad-Toh, Claire M Wade, Tarjei S. Mikkelsen, Elinor K. Karlsson, David B. Jaffe et al. Genome sequence, comparative analysis and haplotype structure of the domestic dog. Nature 438, 803-819 (8 December 2005) 2. Heidi G. Parker, Bridgett M. VonHoldt, Pascale Quignon, Elliott H. Margulies et al. An Expressed Fgf4 Retrogene Is Associated with Breed-Defining Chondrodysplasia in Domestic Dogs. Science 325(5943) 995-998 (21 August 2009)

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