Biocom online cat 16

electrophoresis

life science

• HORIZONTAL GEL SYSTEMS • VERTICAL GEL SYSTEMS • BLOTTING OPTIONS • POWER SUPPLIES • GEL VISUALISATION & DOCUMENTATION • MICROCENTRIFUGES • HYBRIDISATION INCUBATORS • ROCKERS & SHAKERS • THERMAL CYCLERS • SINGLE & MULTICHANNEL PIPETTES • HEATING & COOLING • SAMPLE STORAGE AND LABELING • BALANCES

multisub setting a new standard in horizontal electrophoresis

INJECTION MOULDED CONSTRUCTION - durable, leak-proof environment for complete safety and long life CASSETTE TYPE ELECTRODES - inexpensive, easy to replace.

Made of 99.99% corrosion resistant, pure platinum ELECTRICAL SAFETY - lid can be located in one way only. On removal, power is disconnected from buffer chamber MULTIPLE GEL TRAYS - eliminate the need for additional gel tanks UNIQUE GEL COOLING SYSTEM EASY CLICK LID REMOVAL

Components of multiSUB gel chambers

“plug and go” gel casting Casting dams allow gels to be rapidly cast externally while the multiSUB™ unit is in use for gel running

These comb/guides combine the convenience of a regular comb with an appropriate guide (same teeth number and width) for loading samples, simply flip over.

USB powered extraction fan

combi-comb/loading guides

unique clearsight ClearSight lids solve the condensation build-up problem and so provide a perfectly clear view of the gel and the dye lane progression during the run. This is achieved using a USB powered extraction fan within the lid. Clearsight lids are available as components of complete systems or as upgrades.

All units are CE marked and are in conformity with the relevant CE Safety Directives: 73/23/EEC: LOW VOLTAGE DIRECTIVE: IEC 61010-1:2001. EN 61010-1:2001; 89/336/EEC EMC DIRECTIVE: EN55011 Emissions, EN50082-1 Immunity.

1 2 3 4 5 6 7

Power Cables Safety Lid & Viewing Pane Height-adjustable comb UV transparent gel tray Comb slots ‘Plug-and-Go’ casting dams Colour-coded electrodes with power plug connectors 8 Gel Platform 9 Safety lid thumb locators 10 Moulded tank

maxi

midi

choice

mini

Corrosion resistant, 99.99% pure platinum electrodes and gold plated terminals - easily replaced, ensuring minimum repair times

Retractable connectors compatible with most major power supplies

The multiSUB series of Horizontal Gel Units offers the most versatile solution for DNA and RNA agarose gel electrophoresis currently available. All five units offer an unsurpassed combination of economy of gel and buffer volume, with gel size and sample number versatility. Gel size and sample number requirements can be exactly matched in each unit, with the option of additional gel tray sizes. This eliminates the need for multiple gel tanks for changes in gel size or application.

All units feature removable UV transparent trays. For optimum value and versatility, systems are available with one, two or three tray options (dependent on model). Easy to use, leak proof ‘plug and go’ gel casting dams are included as standard to allow gels to be rapidly cast whilst the multiSUB unit is in use for gel running. With no indentations or casting gate grooves in the tray to interfere with sample progression, traditional tape casting can be used, should this be preferred.

Although lid connectors are compatible with most major power supplies, adapters are available to provide complete compatibility.

combs • five thicknesses, colour coded black: white: red: blue:

0.75mm for ultra resolved bands 1mm supplied as standard 1.5mm for maximising sample volume 2mm for maximising sample volume

• options for Sample Prep • options for Multi-Channel Pipette Compatible The number of samples can be maximised using high tooth number combs White PVC platform cover and red loading guides can be optionally applied to enhance well visibility Trays are UV transparent

Gel trays are free of indentations or casting gate channels which can interfere with sample progression

UV transparent Gel Scoops, available for each unit size, offer a safe and easy method to transfer gels to a UV Transilluminator

s e t t i n g a n e w sta n da r d i n h o r i zo n ta l e l e ct r o p h o r e s i s

multisub selection guide

MSMINI

MSMIDI

MSCHOICE

MSCHOICEST

The preferred option for quick sample checks, particularly following restriction digestion during cloning. Its slim tray fomat makes the MSMINI a very economical choice for separation of up to 64 samples.

These units offer the same trays lengths, but in a wider format, to run more samples just as economically as in the MSMINI under similar running conditions.

Wide format system offers 2-3 times the sample capacity as the MSMINI and MSMIDI units. A comprehensive selection of combs provides full flexibility for preparatory and screening techniques. Suitable for cloning and mini-prep analysis.

A ‘stretched’ version of the MSCHOICE, this system is perfect for extended highresolution separations of samples from 96-well microplates and blocks, using its 15x20cm and 15x25cm gel trays and four 28-sample multichannel compatible combs. May also be used for routine preparatory techniques.

Unit Dimensions (w x l x h)

9 x 21 x 9cm

12.5 x 22 x 9cm

17.5 x 26.5 x 9cm

17.5 x 41 x 9cm

Active Gel Size (w x l) / Corresponding Gel Tray

7x7cm / MS7-UV7 7x10cm / MS7-UV10

10x7cm / MS10-UV7 10x10cm / MS10-UV10

15x7cm / MS15-UV7 15x10cm / MS15-UV10 15x15cm / MS15-UV20

15x20cm / MS15- UVST20 15x25cm / MS15- UVST25

Sample Capacity

1-32 (7x7cm) a 1-64 (7x10cm) b

1-50 (10x7cm) a 1-100 (10x10cm) b

1-70 (15x7cm) a 1-140 (15x10cm) b 1-210 (15x15cm) c

1-280 (15x20cm) † 1-350 (15x25cm) ††

Tank Buffer Volume

225ml

300ml

500ml

1000ml

Buffer Recirculation

No

No

Recommended for high voltage applications or extended runs.

Requires modified lid with 2 buffer recirculation ports. Available on request as per part numbers below: MS15LID-BP

MS15STLID-BP

Plug-and-Go Casting Dams Supplied

Yes, 1 pair

Yes, 1 pair

Yes, 1 pair

Yes, 1 pair

Flexicaster Options & Tray Capacity

MS7/10-FC: 1 tray MS15/20-FC: 2 trays* MS26-FC: 3 trays*

MS7/10-FC: 1 tray MS15/20-FC: 1 tray MS26-FC: 3 trays*

MS15/20-FC: 1 tray MS26-FC: 1 tray

MS15/20-FC: 1 tray MS26-FC: 1 tray

Typical Running Conditions

80V, 45-60 minutes

90V, 45-60 minutes

90-150V, 60-90 minutes

100-150V, 60-90 minutes

Bromophenol Blue Migration

~4-5cm/h at 80V

~4-5cm/h at 90V

~4-7cm/h at 90-150V

~4-6cm/h at 100-150V

a Assumes 1-2 combs per gel; b Assumes 1-4 combs per gel;; c Assumes 1-6 combs per gel;; d Assumes 1-9 combs per gel; e Assumes 1-11 combs per gel; f Assumes 1-12 combs per gel; † Assumes 1-8 combs per gel;; †† Assumes 1-10 combs per gel

4

* Assumes multiSUB™ trays are of the same length (e.g two MS7-UV7) and arranged side-by-side.

MSMAXI

MSSCREEN

RAPIDE

MSMIDI96

MULTISUB-4

Large-format system is versatile enough to be used for routine preparatory and high-throughput techniques. Combs with spacing compatible with fixed multichannel pipettes simplify loading from 96-well microplates and thermal cycler blocks. Suitable for RFLP analysis, Southern and northern blotting applications, plus DNA library and PCR fragment analyses.

Supplied with six 28-sample multichannel compatible combs, with well-spacing that favours direct loading. The content of at least one 96-well microplate or thermal cycler block may be screened in a single gel. Standard combs may be exchanged for 56sample versions to double the sample capacity. A dedicated system for all high-throughput screening applications.

An ultra-compact self-contained system for routine molecular biology procedures and quick checks of samples. Buffer and gel volumes kept to a minimum to maximise current and separation speed.

96-well comb block and specialised tray replicate the format and well-spacing of microplates and thermal cycler blocks. Offset well configuration and short separation distance (1.8cm) allow samples to be run within 15-30 minutes. Ideal system for rapid screening of up to 96 PCRfragment length polymorphisms.

Stretch versions available with extended 3.6cm separation distance to double capacity.

Separates up to 1200 samples in as little as 15 minutes, over a short 1cm separation distance, in 4 stacked horizontal gel trays. A relatively small footprint area, plus the capacity to run 4 gels in 1 tank, saves on valuable laboratory bench space as well as resources.

23 x 39.5 x 9cm

28 x 50 x 9cm

15 x 15 x 4cm

12.5 x 22 x 9cm (MIDI96) 12.5 x 46.5 x 8cm (MIDI96ST)

11 x 35 x 16cm

20x10cm / MS20-UV10 20x15cm / MS20-UV15 20x20cm / MS20-UV20 20x25cm / MS20-UV25

26x16cm / MS26-UV16 26x24cm / MS26-UV24 26x32cm / MS26-UV32

10x8cm / in-built tray

10x12cm / MS10-UV96 10x24cm / MS10- UV96ST

8x6cm / MSUB4UV6 8x12cm / MSUB4UV12 8x18cm / MSUB4UV18

1-200 (20x10cm) b 1-350 (20x15cm) c 1-450 (20x20cm) d 1-550 (20x25cm) e

28-336 (26x16cm) c 28-504 (26x24cm) e 28-672 (26x32cm) f

1-40 (10x8cm) a

96 samples plus 12 (1 lane) or 24 (2 lanes) marker wells

8-126 (8x6cm) g 8-198 (8x12cm) e 8-306 (8x24cm) h

1200ml

1400ml

50ml

300ml (MSMIDI96) 700ml (MIDI96ST)

200ml: 1 tray 400ml: 2 trays 600ml: 3 trays 800ml: 4 trays

As for MSCHOICE and MSCHOICEST

MS20LID-BP Yes – buffer recirculation ports included as standard

No

No

No

Yes, 1 pair

No – supplied with dedicated MSSCREEN flexicaster; See MS26-FC

No

No

No

MS15/20-FC: 1 tray MS26-FC: 1 tray

MS26-FC: 1 tray

No

No

MSUB4EXCASTER: 4 trays

100-150V, 60-90 minutes

100-150V, 90-120 minutes

50V, 30-60 minutes

90V, 15-30 minutes

100-150V, 15-30 minutes

~4-6.5cm/h at 100-150V

~4-6cm/h at 100-150V

~4cm/h at 50V

~4-5cm/h at 90V

~4-6cm/hr at 100-150V

5

• AVAILABLE wITH 7 X 7CM, 7 X 10CM OR wITH BOTH GEL TRAyS

• ECONOMIC

LOw GEL AND

BUFFER VOLUMES

• SMALL

LAB BENCH FOOTPRINT

MINI

multisub multiSUB Mini is designed for low to medium numbers of samples.

The small gel size maximises run economy but does not compromise versatility as two tray options are available – 7 x 7cm and 7 x 10cm, and combs ranging from preparative up to 16 samples. Simply by altering the gel tray or comb, this compact unit is capable of resolving up to 64 different samples, prepping 1ml of sample or separating sample bands over a distance of 9cm.

Technical Specifications GEL DIMENSIONS, (W X L)

7 X 7CM 7 X 10CM

UNIT DIMENSIONS

21 X 9 X 9CM (W X D X H)

MAX. SAMPLE CAPACITY

7 X 7CM TRAY – 32 SAMPLES 7 X 10CM TRAY – 64 SAMPLES

BUFFER VOLUME

225ML

COMBS AVAILABLE : NO. OF SAMPLES THICKNESSES

1, 2, 4, 8MC, 8, 10, 12MC, 16

Buffer Saver Blocks physically reduce the volume of a gel chamber and so reduce buffer requirements, saving cost

0.75, 1, 1.5, 2MM

Typical Applications Designed for quick checks of low to medium numbers of samples

Flexicaster Mini/Midi allows casting of gel lengths up to 10cm

components of multiSUB Mini Duo molecular grade agarose, see page 24

6

BCMSMINI7 BCMSMINI10 BCMSMINIDUO MS7-UV7 MS7-UV10 MS7-PE MS7-NE MS7-UVDAM

multiSUB Mini, 7 x 7cm UV Tray, 2 x 8 sample combs, loading guides and dams multiSUB Mini, 7 x 10cm UV Tray, 2 x 8 sample combs, loading guides and dams multiSUB Mini, 7 x 7cm & 7 x 10cm UV Tray, 2 x 8 sample combs, loading guides and dams 7 x 7cm UV Tray MS7-LG Adhesive Loading Guides 7 x 10cm UV Tray MS7-WP Viewing Platform Positive Electrode MS7-UVS 7cm UV Gel Scoop Negative Electrode MSMINICP Cool-pack and Platform Casting Dams, pk/2 MSMINIBSB Buffer Saver Blocks, pk/2,saves 100ml of buffer

CODE

DESCRIPTION

MS7-1-0.75 MS7-2-0.75 MS7-4-0.75 MS7-8MC-0.75 MS7-8-0.75 MS7-10-0.75 MS7-12MC-0.75 MS7-16-0.75

Comb Prep 1, Marker 1, 0.75mm thick Comb Prep 2, Marker 2, 0.75mm thick Comb Prep 4, Marker 2, 0.75mm thick Comb 8 sample MC, 0.75mm thick Comb 8 sample, 0.75mm thick Comb 10 sample, 0.75mm thick Comb 12 sample MC, 0.75mm thick Comb 16 sample, 0.75mm thick

MS7-1-1 MS7-2-1 MS7-4-1 MS7-8MC-1 MS7-8-1 MS7-10-1 MS7-12MC-1 MS7-16-1

Comb Prep 1, Marker 1, 1mm thick Comb Prep 2, Marker 2, 1mm thick Comb Prep 4, Marker 2, 1mm thick Comb 8 sample MC, 1mm thick Comb 8 sample, 1mm thick Comb 10 sample, 1mm thick Comb 12 sample MC, 1mm thick Comb 16 sample, 1mm thick

SAMPLE VOLUME FOR A 5MM THICk GEL

Colour

Colour

ORDERING INFORMATION

MS7/10-FC BCMSMINIXCS

multiSUB Mini/Midi Flexi caster ClearSight multiSUB MiniX, as above with Fan & power source where ‘X’ should be replaced with ‘7’, ‘10’ or ‘DUO’

SAMPLE VOLUME FOR A 5MM THICk GEL

CODE

DESCRIPTION

152µl 68µl 36µl 8µl 19µl 14µl 10µl 7µl

MS7-1-1.5 MS7-2-1.5 MS7-4-1.5 MS7-8MC-1.5 MS7-8-1.5 MS7-10-1.5 MS7-12MC-1.5 MS7-16-1.5

Comb Prep 1, Marker 1, 1.5mm thick Comb Prep 2, Marker 2, 1.5mm thick Comb Prep 4, Marker 2, 1.5mm thick Comb 8 sample MC, 1.5mm thick Comb 8 sample, 1.5mm thick Comb 10 sample, 1.5mm thick Comb 12 sample MC, 1.5mm thick Comb 16 sample, 1.5mm thick

304µl 135µl 72µl 17µl 37µl 27µl 20µl 15µl

203µl 90µl 48µl 11µl 25µl 18µl 14µl 10µl

MS7-1-2 MS7-2-2 MS7-4-2 MS7-8MC-2 MS7-8-2 MS7-10-2 MS7-12MC-2 MS7-16-2

Comb Prep 1, Marker 1, 2mm thick Comb Prep 2, Marker 2, 2mm thick Comb Prep 4, Marker 2, 2mm thick Comb 8 sample MC, 2mm thick Comb 8 sample, 2mm thick Comb 10 sample, 2mm thick Comb 12 sample MC, 2mm thick Comb 16 sample, 2mm thick

405µl 180µl 96µl 23µl 50µl 36µl 27µl 20µl

MC = multichannel pipette compatible

MIDI

With gel tray options of 10 x 7cm and 10 x 10cm, multiSUB Midi has been designed for routine horizontal gel electrophoresis.

• AVAILABLE wITH 10 X 7CM, 10

Extending only the width of this unit allows more samples to be resolved per gel than the multiSUB Mini without a significant increase in buffer or gel volumes.

X

10CM

OR wITH BOTH

GEL TRAyS

• RUN UP TO 100 SAMPLES • LOw BUFFER VOLUMES • IDEAL FOR RAPID

A maximum of 100 samples per gel can be resolved making this unit ideal for those routinely checking medium numbers of samples over short to medium gel run lengths.

ELECTROPHORESIS

Technical Specifications

for Power Supplies and Product Selector see pages 48-49 adhesive loading guides allow easy well identification and sample loading

casting dams allow gels to be rapidly cast externally while the multiSUB™ unit is in use for gel running

GEL DIMENSIONS, (W X L)

10 X 7CM 10 X 10CM

UNIT DIMENSIONS

22 X 12.5 X 9CM (W X D X H)

MAX. SAMPLE CAPACITY

10 X 7CM TRAY – 50 SAMPLES 10 X 10CM TRAY – 100 SAMPLES

BUFFER VOLUME

300ML

COMBS AVAILABLE : NO. OF SAMPLES 1, 2, 4, 8, 10MC, 12, 16, 20MC, 25 0.75, 1, 1.5, 2MM THICKNESSES

Typical Applications Ideal for quick checks of samples from PCR and cloning

reversible loading guides sit directly above each well to provide a convenient loading template for single- and multi-channel pipettes

BCMSMIDI7 BCMSMIDI10 BCMSMIDIDUO MS10-UV7 MS10-UV10 MS10-PE MS10-NE MS10-UVDAM

multiSUB Midi, 10 x 7cm UV Tray, 2 x 16 sample combs, loading guides and dams multiSUB Midi, 10 x 10cm UV Tray, 2 x 16 sample combs, loading guides and dams multiSUB Midi, 10 x 7cm & 10 x 10cm UV Tray, 2 x 16 sample combs, loading guides and dams MS10-LG Adhesive Loading Guides 10 x 7cm UV Tray MS10-WP Viewing Platform 10 x 10cm UV Tray MS10-UVS 10cm UV Gel Scoop Positive Electrode MS7/10-FC multiSUB Mini/Midi Flexicaster Negative Electrode MSMIDICP Cool-Pack and Platform Casting Dams, pk/2

CODE

DESCRIPTION

MS10-1-0.75 MS10-2-0.75 MS10-4-0.75 MS10-8-0.75 MS10-10MC-0.75 MS10-12-0.75 MS10-16-0.75 MS10-20MC-0.75 MS10-25-0.75

Comb Prep 1, Marker 1, 0.75mm thick Comb Prep 2, Marker 2, 0.75mm thick Comb Prep 4, Marker 2, 0.75mm thick Comb 8 sample, 0.75mm thick Comb 10 sample MC, 0.75mm thick Comb 12 sample, 0.75mm thick Comb 16 sample, 0.75mm thick Comb 20 sample MC, 0.75mm thick Comb 25 sample, 0.75mm thick

MS10-1-1 MS10-2-1 MS10-4-1 MS10-8-1 MS10-10MC-1 MS10-12-1 MS10-16-1 MS10-20MC-1 MS10-25-1

Comb Prep 1, Marker 1, 1mm thick Comb Prep 2, Marker 2, 1mm thick Comb Prep 4, Marker 2, 1mm thick Comb 8 sample, 1mm thick Comb 10 sample MC, 1mm thick Comb 12 sample, 1mm thick Comb 16 sample, 1mm thick Comb 20 sample MC, 1mm thick Comb 25 sample, 1mm thick

SAMPLE VOLUME FOR A 5MM THICk GEL

Colour

Colour

ORDERING INFORMATION

MSMIDIBSB BCMSMIDIXCS

Buffer Saver Blocks, pk/2, saves 100ml of buffer ClearSight multiSUB MidiX, as above with Fan & power source where ‘X’ should be replaced with ‘7’, ‘10’ or ‘DUO’

SAMPLE VOLUME FOR A 5MM THICk GEL

CODE

DESCRIPTION

270µl 118µl 57µl 30µl 20µl 17µl 12µl 10µl 7µl

MS10-1-1.5 MS10-2-1.5 MS10-4-1.5 MS10-8-1.5 MS10-10MC-1.5 MS10-12-1.5 MS10-16-1.5 MS10-20MC-1.5 MS10-25-1.5

Comb Prep 1, Marker 1, 1.5mm thick Comb Prep 2, Marker 2, 1.5mm thick Comb Prep 4, Marker 2, 1.5mm thick Comb 8 sample, 1.5mm thick Comb 10 sample MC, 1.5mm thick Comb 12 sample, 1.5mm thick Comb 16 sample, 1.5mm thick Comb 20 sample MC, 1.5mm thick Comb 25 sample, 1.5mm thick

540µl 236µl 115µl 61µl 41µl 34µl 24µl 20µl 15µl

360µl 158µl 77µl 41µl 27µl 23µl 16µl 14µl 10µl

MS10-1-2 MS10-2-2 MS10-4-2 MS10-8-2 MS10-10MC-2 MS10-12-2 MS10-16-2 MS10-20MC-2 MS10-25-2

Comb Prep 1, Marker 1, 2mm thick Comb Prep 2, Marker 2, 2mm thick Comb Prep 4, Marker 2, 2mm thick Comb 8 sample, 2mm thick Comb 10 sample, 2mm thick Comb 12 sample, 2mm thick Comb 16 sample, 2mm thick Comb 20 sample MC, 2mm thick Comb 25 sample, 2mm thick

720µl 315µl 153µl 81µl 54µl 45µl 32µl 27µl 20µl MC = multichannel pipette compatible

7

• THREE

TRAy OPTIONS

• RUN

UP TO

210

• LOw

BUFFER VOLUMES

• MULTICHANNEL

SAMPLES

CHOICE

multisub PIPETTE

COMPATIBLE COMBS FOR SPEED LOADING

Technical Specifications GEL DIMENSIONS, 15 X 7CM; 15 X 10CM; 15 X 15CM (TRIO) 15 X 20CM; 15 X 25CM (STRETCH MODELS) (W X L) UNIT DIMENSIONS 26.5 X 17.5 X 9CM (TRIO) (W X D X H) 26.5 X 41 X 9CM (STRETCH MODELS) MAX. SAMPLE

15 X 7CM TRAY – 70 SAMPLES CAPACITY 15 X 10CM TRAY – 140 SAMPLES 15 X 15CM TRAY – 210 SAMPLES 15 X 20CM TRAY – 280 SAMPLES 15 X 25CM TRAY – 350 SAMPLES

BUFFER VOLUME

500ML; 1000ML (STRETCH)

multiSUB Choice offers a wide degree of versatility. Three tray options are available - 15 x 7cm, 15 x 10cm and 15 x 15cm - allowing the choice of one, two or all three gel length options at the time of purchase. Maximising comb and tray options allow up to 210 samples to be resolved per gel.

The 15cm total run length allows restriction fragment or other close MW sample bands to be easily separated and identified. Speed loading is accomplished using 10, 14, 16, 18, 28 and 30 sample multi-channel pipette compatible combs. All models include loading guides and casting dams.

Fabricated STRETCH units are available with optional 15 x 20cm and 15 x 25cm gel trays and four 28-sample combs for those wishing to perform higher resolution separation of more samples over a longer distance. multiSUB Choice Trio includes all 3 tray sizes for optimum versatility and value

COMBS AVAILABLE: NO. OF SAMPLES 1, 2, 4, 10, 10MC, 12, 14MC, 16MC, 18MC, 20, 28MC, 30MC THICKNESSES 0.75, 1, 1.5, 2MM

Typical Applications Ideal for restriction fragment analysis, sample prep or checking of high numbers of samples

multiSUB Choice Stretch

ORDERING INFORMATION

BCMSCHOICETRIO multiSUB Choice Trio, 15 x 7,10 & 15cm UV Tray, 2 x 20 sample combs* BCMSCHOICEST20 multiSUB Stretch20, 15 x 20cm UV Tray, 4 x28 sample combs* BCMSCHOICEST25 multiSUB Stretch25, 15 x 25cm UV Tray, 4 x28 sample combs*

MS15-UV7 MS15-UV10 MS15-UV15 MS15-UVST20 MS15-UVST25 MS15-UVDAM

15 x 7cm UV Tray 15 x 10cm UV Tray 15 x 15cm UV Tray 15 x 20 cm UV Tray 15 x 25 cm UV Tray Casting Dams, pk/2

MS15-PE MS15-NE MS15-LG MS15-UVS MSCHOICECP MSCHOICEBSB

Positive Electrode Negative Electrode Adhesive Loading Guides 15cm UV Gel Scoop Cool-Pack and Platform Buffer Saver Blocks pk/2, saves 190ml of buffer

CODE

DESCRIPTION

SAMPLE VOLUME FOR A 5MM THICk GEL

MS15-1-0.75 MS15-2-0.75 MS15-4-0.75 MS15-10-0.75 MS15-12-0.75 MS15-20-0.75 MS15-35-0.75 MS15-10MC-0.75 MS15-14MC-0.75 MS15-16MC-0.75 MS15-18MC-0.75 MS15-28MC-0.75 MS15-30MC-0.75

Comb Prep 1, Marker 1, 0.75mm thick Comb Prep 2, Marker 2, 0.75mm thick Comb Prep 4, Marker 2, 0.75mm thick Comb 10 sample, 0.75mm thick Comb 12 sample, 0.75mm thick Comb 20 sample, 0.75mm thick Comb 35 sample, 0.75mm thick Comb 10 sample MC, 0.75mm thick Comb 14 sample MC, 0.75mm thick Comb 16 sample MC, 0.75mm thick (DuoComb - 17MC on reverse) Comb 18 sample MC, 0.75mm thick (DuoComb - 17MC on reverse) Comb 28 sample MC, 0.75mm thick Comb 30 sample MC, 0.75mm thick

MS15-1-1 MS15-2-1 MS15-4-1 MS15-10-1 MS15-12-1 MS15-20-1 MS15-35-1 MS15-10MC-1 MS15-14MC-1 MS15-16MC-1 MS15-18MC-1 MS15-28MC-1 MS15-30MC-1

Comb Prep 1, Marker 1, 1mm thick Comb Prep 2, Marker 2, 1mm thick Comb Prep 4, Marker 2, 1mm thick Comb 10 sample, 1mm thick Comb 12 sample, 1mm thick Comb 20 sample, 1mm thick Comb 35 sample, 1mm thick Comb 10 sample MC, 1mm thick Comb 14 sample MC, 1mm thick Comb 16 sample, 1mm thick (DuoComb - 17MC on reverse) Comb 18 sample MC, 1mm thick (DuoComb - 17MC on reverse) Comb 28 sample MC, 1mm thick Comb 30 sample MC, 1mm thick

Colour

Colour

BCMSCHOICE7 multiSUB Choice, 15 x 7cm UV Tray, 2 x 20 sample combs* BCMSCHOICE10 multiSUB Choice, 15 x 10cm UV Tray, 2 x 20 sample combs* BCMSCHOICE15 multiSUB Choice, 15 x 15cm UV Tray, 2 x 20 sample combs*

8

low cost gel documentation system, see page 52-55

MS15/20-FC multiSUB Choice/Maxi Flexi caster BCMSCHOICEXCS ClearSight multiSUB ChoiceX, as above with Fan & power source. where ‘X’ should be replaced with ‘7’, ‘10’, ‘15’, ‘20’, ‘25’ or ‘TR’

SAMPLE VOLUME FOR A 5MM THICk GEL

Code

Description

371µl 169µl 91µl 34µl 30µl 16µl 7µl 22µl 22µl 20µl 8µl 8µl 9µl

MS15-1-1.5 MS15-2-1.5 MS15-4-1.5 MS15-10-1.5 MS15-12-1.5 MS15-20-1.5 MS15-35-1.5 MS15-10MC-1.5 MS15-14MC-1.5 MS15-16MC-1.5 MS15-18MC-1.5 MS15-28MC-1.5 MS15-30MC-1.5

Comb Prep 1, Marker 1, 1.5mm thick Comb Prep 2, Marker 2, 1.5mm thick Comb Prep 4, Marker 2, 1.5mm thick Comb 10 sample, 1.5mm thick Comb 12 sample, 1.5mm thick Comb 20 sample, 1.5mm thick Comb 35 sample, 1.5mm thick Comb 10 sample MC, 1.5mm thick Comb 14 sample MC, 1.5mm thick Comb 16 sample, 1.5mm thick (DuoComb - 17MC on reverse) Comb 18 sample MC, 1.5mm thick (DuoComb - 17MC on reverse) Comb 28 sample MC, 1.5mm thick Comb 30 sample MC, 1.5mm thick

743µl 338µl 182µl 68µl 61µl 32µl 15µl 44µl 44µl 41µl 41µl 17µl 17µl

495µl 225µl 122µl 45µl 41µl 21µl 10µl 29µl 29µl 27µl 11µl 11µl 13µl

MS15-1-2 MS15-2-2 MS15-4-2 MS15-10-2 MS15-12-2 MS15-20-2 MS15-35-2 MS15-10MC-2 MS15-14MC-2 MS15-16MC-2 MS15-18MC-2 MS15-28MC-2 MS15-30MC-2

Comb Prep 1, Marker 1, 2mm thick Comb Prep 2, Marker 2, 2mm thick Comb Prep 4, Marker 2, 2mm thick Comb 10 sample, 2mm thick Comb 12 sample, 2mm thick Comb 20 sample, 2mm thick Comb 35 sample, 2mm thick Comb 10 sample MC, 2mm thick Comb 14 sample MC, 2mm thick Comb 16 sample, 2mm thick (DuoComb - 17MC on reverse) Comb 18 sample MC, 2mm thick (DuoComb - 17MC on reverse) Comb 28 sample MC, 2mm thick Comb 30 sample MC, 2mm thick

990µl 450µl 243µl 90µl 81µl 43µl 20µl 59µl 59µl 54µl 23µl 23µl 25µl

* all models include loading guides and casting dams

MAXI

multiSUB Maxi is primarily designed for resolution of high numbers of samples such as from Cloning or PCR. It also

• AVAILABLE wITH 20 X 25CM, 20 X 20CM OR 20 X 10CM

allows ultra high-resolution separations over extended runs.

Tray sizes correspond to standard blotter sizes for easy sample transfer onto a membrane for further analysis.

Four tray sizes are available – 20 x 15cm, 20 x 20cm, 20 x 25cm and a half-length 20 x 10cm. Multichannel pipette compatible combs up to 40 sample facilitate speed loading of up to 440 samples per gel. 50 sample combs offers a maximum sample capacity of 550 samples per gel.

GEL TRAyS

• RUN

UP TO

• LOw

BUFFER VOLUMES

• IDEAL

550

SAMPLES

FOR EXTENDED

SEPARATIONS

Technical Specifications Buffer Saver Blocks physically reduce the volume of a gel chamber and so reduce buffer requirements, saving running costs

GEL DIMENSIONS, (W X L)

20 X 10CM 20 X 20CM

UNIT DIMENSIONS

39.5 X 23 X 9CM (W X D X H)

MAX. SAMPLE

20 X 10CM TRAY – 200 SAMPLES 20 X 15CM TRAY – 350 SAMPLES 20 X 20CM TRAY – 450 SAMPLES 20 X 25CM TRAY – 550 SAMPLES

CAPACITY

BUFFER VOLUME

20 X 15CM 20 X 25CM

1500ML

COMBS AVAILABLE : NO. OF SAMPLES 1, 2, 4, 10, 16, 20MC, 25, 30, 36, 40MC, 50 THICKNESSES 0.75, 1, 1.5, 2MM

Typical Applications

This Gel Levelling Table provides a useful way of pouring gels of uniform thickness. The table features a large 32 x 26cm platform with large thumb-wheel levelling feet at each corner. A levelling bubble is supplied.

ORDERING INFORMATION

multiSUB Maxi, 20 x 10cm UV Tray, 2 x 20 sample combs* multiSUB Maxi, 20 x 15cm UV Tray, 2 x 20 sample combs* multiSUB Maxi, 20 x 20cm UV Tray, 2 x 20 sample combs* MS20-PE 20 x 10cm UV Tray MS20-NE 20 x 15cm UV Tray MS20-UVS 20 x 20cm UV Tray MSMAXICP 20 x 25cm UV Tray Casting Dams, pk/2 MSMAXIBSB Adhesive Loading Guides MS15/20-FC

CODE

DESCRIPTION

MS20-1-0.75 MS20-2-0.75 MS20-4-0.75 MS20-10-0.75 MS20-16-0.75 MS20-20MC-0.75 MS20-25-0.75 MS20-30-0.75 MS20-36-0.75 MS20-40MC-0.75 MS20-50-0.75

Comb Prep 1, Marker 1, 0.75mm thick Comb Prep 2, Marker 2, 0.75mm thick Comb Prep 4, Marker 2, 0.75mm thick Comb 10 sample, 0.75mm thick Comb 16 sample, 0.75mm thick Comb 20 sample MC, 0.75mm thick Comb 25 sample, 0.75mm thick Comb 30 sample, 0.75mm thick Comb 36 sample, 0.75mm thick Comb 40 sample MC, 0.75mm thick Comb 50 sample, 0.75mm thick

MS20-1-1 MS20-2-1 MS20-4-1 MS20-10-1 MS20-16-1 MS20-20MC-1 MS20-25-1 MS20-30-1 MS20-36-1 MS20-40MC-1 MS20-50-1

Comb Prep 1, Marker 1, 1mm thick Comb Prep 2, Marker 2, 1mm thick Comb Prep 4, Marker 2, 1mm thick Comb 10 sample, 1mm thick Comb 16 sample, 1mm thick Comb 20 sample MC, 1mm thick Comb 25 sample, 1mm thick Comb 30 sample, 1mm thick Comb 36 sample, 1mm thick Comb 40 sample MC, 1mm thick Comb 50 sample, 1mm thick

* all models include loading guides and casting dams

BCMSMAXI25 multiSUB Maxi, 20 x 25cm UV Tray, 2 x 20 sample combs* BCMSMAXIDUO multiSUB Maxi, 20 x 10 & 20 x 20cm UV Tray, 2 x 20 sample combs* Positive Electrode Negative Electrode 20cm UV Gel Scoop Cool-Pack and Platform Buffer Saver Blocks pk/2, saves 450ml of buffer multiSUB Choice/Maxi Flexi caster

SAMPLE VOLUME FOR A 5MM THICk GEL

Colour

BCMSMAXI10 BCMSMAXI15 BCMSMAXI20 MS20-UV10 MS20-UV15 MS20-UV20 MS20-UV25 MS20-UVDAM MS20-LG

Primarily designed for separating high numbers of samples from PCR or cloning

GLT BCMSMAXIXCS

Gel Levelling Table ClearSight multiSUB MaxiX, as above with Fan & power source where ‘X’ should be replaced with ‘10’, ‘15’, ‘20’, ‘25’ or ‘DUO’

SAMPLE VOLUME FOR A 5MM THICk GEL

CODE

DESCRIPTION

506µl 236µl 115µl 54µl 30µl 20µl 16µl 13µl 11µl 8µl 8µl

MS20-1-1.5 MS20-2-1.5 MS20-4-1.5 MS20-10-1.5 MS20-16-1.5 MS20-20MC-1.5 MS20-25-1.5 MS20-30-1.5 MS20-36-1.5 MS20-40MC-1.5 MS20-50-1.5

Comb Prep 1, Marker 1, 1.5mm thick Comb Prep 2, Marker 2, 1.5mm thick Comb Prep 4, Marker 2, 1.5mm thick Comb 10 sample, 1.5mm thick Comb 16 sample, 1.5mm thick Comb 20 sample MC, 1.5mm thick Comb 25 sample, 1.5mm thick Comb 30 sample, 1.5mm thick Comb 36 sample, 1.5mm thick Comb 40 sample MC, 1.5mm thick Comb 50 sample, 1.5mm thick

1013µl 473µl 230µl 108µl 61µl 41µl 32µl 26µl 22µl 17µl 16µl

675µl 315µl 153µl 72µl 41µl 27µl 21µl 17µl 14µl 11µl 10µl

MS20-1-2 MS20-2-2 MS20-4-2 MS20-10-2 MS20-16-2 MS20-20MC-2 MS20-25-2 MS20-30-2 MS20-36-2 MS20-40MC-2 MS20-50-2

Comb Prep 1, Marker 1, 2mm thick Comb Prep 2, Marker 2, 2mm thick Comb Prep 4, Marker 2, 2mm thick Comb 10 sample, 2mm thick Comb 16 sample, 2mm thick Comb 20 sample Mc, 2mm thick Comb 25 sample, 2mm thick Comb 30 sample, 2mm thick Comb 36 sample, 2mm thick Comb 40 sample MC, 2mm thick Comb 50 sample, 2mm thick

1350µl 630µl 306µl 144µl 81µl 54µl 42µl 34µl 29µl 23µl 21µl

MC = multichannel pipette compatible

9

SCREEN

multisub • AVAILABLE wITH 26 X 16, 26 X 24 AND 26 X 32CM OR ALL THREE GEL TRAyS

• RUN

UP TO

672

• LOw

BUFFER VOLUMES

• MULTICHANNEL

SAMPLES

PIPETTE

COMPATIBLE COMBS FOR SPEED LOADING

Designed for rapid screening of very large numbers of Clonal or PCR samples, multiSUB Screen horizontal gel unit has a 672 maximum sample capacity per gel. This allows loading and analysis of exactly seven 96 well format micro titre plates.

Technical Specifications GEL DIMENSIONS, (W X L)

26 X 32CM 26 X 24CM 26 X 16CM

UNIT DIMENSIONS

50 X 28 X 9CM (W X D X H)

MAX. SAMPLE

26 X 32CM TRAY – 672 SAMPLES 26 X 24CM TRAY – 504 SAMPLES 26 X 16CM TRAY – 336 SAMPLES

CAPACITY

BUFFER VOLUME

1400ML

COMBS AVAILABLE : NO. OF SAMPLES 28MC, 56MC THICKNESSES 0.75, 1, 1.5, 2MM

The large gel run length of 32cm also allows resolution of samples over a long distance for separation of complex sample bands such as in restriction fragment analysis.

Flexicaster now included with all models of multiSUB Screen

The unit is available with a full length tray or with other tray length options of 16 or 24cm so that the user’s exact requirements can be matched. In addition to options for single length gel trays, multiSUB Screen is available with all three gel tray lengths to provide the maximum in flexibility, versatility and value.

All models of multiSUB Screen are now supplied with Flexicaster MS26-FC as standard. Buffer recirculation ports are also included as standard to allow enhanced resolution over extended runs while loading guides enhance well visibility for easy sample loading.

all multiSUB Screen combs are multichannel pipette compatible

scoops for safe transfer of gels

Typical Applications Ideal for checking very large numbers of samples or extended high resolution separations

for Power Supplies and Product Selector see page 48

BCMSSCRN16 BCMSSCRN24 BCMSSCRN32 BCMSSCRNTRIO MS26-UV16 MS26-UV24 MS26-UV32 MS26-LG MS26-UVS

multiSUB Screen, 26 x 16cm UV Tray, 6 x 28 sample combs, loading guides and Flexicaster multiSUB Screen, 26 x 24cm UV Tray, 6 x 28 sample combs, loading guides and Flexicaster multiSUB Screen, 26 x 32cm UV Tray, 6 x 28 sample combs, loading guides and Flexicaster multiSUB Screen, 26 x 16cm, 26 x 24cm, 26 x 32cm UV Trays, 6 x 28 sample combs, loading guides and Flexicaster 26 x 16cm UV Tray MS26-PE Positive Electrode MSSCRNBSB 26 x 24cm UV Tray MS26-NE Negative Electrode MS26-FC 26 x 32cm UV Tray MSSCRNCP Cool-Pack and Platform Adhesive Loading Guides PP01 Single Channel Peristaltic Pump, 30-100rpm 26cm UV Gel Scoop MSBRP Buffer Recirculation Ports

CODE

DESCRIPTION

MS26-28MC-0.75 MS26-56MC-0.75

Comb 28 sample MC, 0.75mm thick Comb 56 sample MC, 0.75mm thick

MS26-28MC-1 MS26-56MC-1

Comb 28 sample MC, 1mm thick Comb 56 sample MC, 1mm thick

10

SAMPLE VOLUME FOR A 5MM THICk GEL

Colour

Colour

ORDERING INFORMATION

Buffer Saver Blocks, pk/2 saves 625ml of buffer Flexicaster for gels up to 32cm. Casts 7, 10, 15, 16, 20, 24 and 32cm long gels

SAMPLE VOLUME FOR A 5MM THICk GEL

CODE

DESCRIPTION

25µl 10µl

MS26-28MC-1.5 MS26-56MC-1.5

Comb 28 sample MC, 1.5mm thick Comb 56 sample MC, 1.5mm thick

51µl 20µl

34µl 14µl

MS26-28MC-2 MS26-56MC-2

Comb 28 sample MC, 2mm thick Comb 56 sample MC, 2mm thick

68µl 27µl

MC = multichannel pipette compatible

MINI RAPIDE

• A CONVENIENT SELF CONTAINED SOLUTION FOR ROUTINE

AGAROSE GEL ELECTROPHORESIS

• ULTRA • LOw

COMPACT

BUFFER VOLUMES

• MULTICHANNEL

PIPETTE

COMPATIBLE

Technical Specifications miniRapide is a completely self contained system designed for quick checks of samples. Gel casting, running and analysis are all performed in the same ultra compact unit with buffer and gel volumes have been kept to a minimum. The parallel electrode arrangement allows ultra efficient current transfer, enabling resolution

to be completed within 30 minutes. The UV transparent base permits direct viewing on a UV Transilluminator with no need for time consuming transfer and potential gel damage.

Dual comb slots allow the loading of up to 40 samples per gel while multichannel pipette compatible combs further enhance the speed and convenience.

GEL DIMENSIONS,

10 X 8CM (W X L)

UNIT DIMENSIONS

15 X 15 X 4CM (W X D X H)

MAX. SAMPLE CAPACITY

40 SAMPLES

BUFFER VOLUME

50ML

COMBS AVAILABLE : NO. OF SAMPLES THICKNESSES

1, 4, 8, 12, 16, 20 1, 1.5MM

Typical Applications For routine, rapid checking of samples

BATTERY GEL • LOw VOLTAGE MAkES THE BATTERy GEL IDEAL FOR USE IN SCHOOLS AND

COLLEGES FOR THE MAXIMUM SAFETy

• INTEGRATED

SAFETy LID PREVENTS

Technical Specifications

ACCESS TO GEL AREA wHEN RUNNING

• UV-TRANSPARENT

ACRyLIC FOR

DIRECT VISUALISATION OF THE GEL wITHIN THE TANk

• SMALL

Battery mini-gel is designed specifically with safety and cost in mind: as no power supply is necessary. Results are obtainable

SIzE MINIMISES BUFFER AND

GEL CONSUMPTION

• COST-EFFECTIVE –

within 1.5 hours and it is ideal for schools, classrooms and electrophoresis in the field where no power source is available.

NO NEED FOR

EXPENSIVE POwER SUPPLIES

GEL DIMENSIONS

6 X 7.5CM (WXL)

UNIT DIMENSIONS

17 X 11 X 3.5CM (WXDXH)

MAX. SAMPLE (CAPACITY)

32 SAMPLES

BUFFER VOLUME

50ML

COMBS AVAILABLE: NO. OF SAMPLES THICKNESSES

4, 8, 12 AND 16 1MM

Typical Applications Teaching and field electrophoresis

miniRapide, 10 x 8cm, 2 x 8 sample combs 1.5mm and casting dams miniRapide Casting Dams, pk/2

CODE

DESCRIPTION

FMMS-1-1 FMMS-4-1 FMMS-8-1 FMMS-12-1 FMMS-16-1 FMMS-20MC-1

Comb 1 sample , 1 dye , 1mm thick Comb 4 sample, 1mm thick Comb 8 sample, 1mm thick Comb 12 sample, 1mm thick Comb 16 sample, 1mm thick Comb 20 sample MC, 1mm thick

MSBGEL

Battery operated horizontal gel 6 x 7.5cm, 2 x 8 sample, 1mm thick combs, casting gates Replacement Platinum Wire 0.2mm – 50cm Casting gates pk/2 for MSBGEL Comb 8 sample, 1mm thick

RPW0.2 MSBGEL-CG MSBG-1-8

SAMPLE VOLUME FOR A 5MM THICk GEL 330µl 90µl 40µl 25µl 15µl 10µl

Colour

Colour

ORDERING INFORMATION BCFMMS10 FMMS-DAM

RPW0.2

Replacement Platinum Wire 0.2mm – 50cm

CODE

DESCRIPTION

FMMS-1-1.5 FMMS-4-1.5 FMMS-8-1.5 FMMS-12-1.5 FMMS-16-1.5 FMMS-20MC-1.5

Comb 1 sample , 1 dye , 1.5mm thick Comb 4 sample, 1.5mm thick Comb 8 sample, 1.5mm thick Comb 12 sample, 1.5mm thick Comb 16 sample, 1.5mm thick Comb 20 sample MC, 1.5mm thick

MSBGEL-BAT MSBG-1-12 MSBG-1-4 MSBG-1-16

PP3 batteries for MSBGEL pk/2 Comb 12 sample, 1mm thick Comb 4 sample, 1mm thick Comb 16 sample, 1mm thick

SAMPLE VOLUME FOR A 5MM THICk GEL 495µl 135µl 60µl 38µl 23µl 15µl

MC = multichannel pipette compatible

11

FOR LOADING OF

MIDI

• DESIGNED

96

high throughput DNA SAMPLES FROM MULTI-

wELL PLATES

• IDEAL

FOR HIGH THROUGHPUT

ELECTROPHORESIS

• AVERAGE RUN-TIME TO 30 MINUTES • DIRECT

IS jUST

15 Higher capacity Stretch models now available

MICROPLATE FORMAT

FOR EASy LANE IDENTIFICATION

• MULTI-CHANNEL

PIPETTE

COMPATIBLE COMBS FOR SPEED

multiSUB Midi 96 Gel System is ideal for high throughput electrophoresis of PCR products or samples containing a small number of DNA bands.

LOADING

Technical Specifications GEL DIMENSIONS, MSMIDI96 (W X L) MSMIDI96ST

10 X 12CM 10 X 24CM

UNIT DIMENSIONS MSMIDI96 (W X D X H) MSMIDI96ST

22 X 12.5 X 9CM 12.5 X 46.5 X 8CM

MAX. SAMPLE CAPACITY

96 SAMPLES PLUS 1 OR 2 MARKER LANES

BUFFER VOLUME (W X L)

MSMIDI96 MSMIDI96ST

Its 10x12cm (W x L) gel dimensions and 96-well comb block format correspond to the standard microplate configuration. Two different types of comb are available: option one which has 8 wells and one additional lane for DNA markers and option two which has 8 wells and 2 additional lanes for markers. The offset layout of the wells allows a maximum run length of 1.8cm per well, resulting in improved separation.

200ML 700ML BLOCKS

COMBS AVAILABLE NO. OF SAMPLES 8 + 1X MARKER, 8 + 2X MARKERS THICKNESSES 1, 1.5MM

multiSUB Midi 96 can accommodate samples from a 96-well plate – samples can be loaded directly using 8-channel pipettes. As with all multiSUB units, leak-proof casting is simplified using the supplied casting dams while their ultra-compact size minimises buffer usage and bench space requirements.

Midi 96 Stretch Systems allow extended run lengths per well of up to 3.6cm, or for loading of samples from two 96-well plates.

Typical Applications Ideal for analysis of up to 96 PCRfragment length polymorphisms loaded from 96-well microplates or thermal cycler blocks After pouring gel, the 96 Samples are loaded using After removing dams, to the gel chamber and well comb is inserted and a multichannel pipette running tray is transferred the lid positioned ready for the gel is allowed to set electrophoresis

ORDERING INFORMATION

BCMSMIDI96 multiSUB Midi 96, UV tray, Comb block with 12 x 8 sample, 1mm thick combs, casting dams BCMSMIDI961.5 multiSUB Midi 96, UV tray, Comb block with 12 x 8 sample, 1.5mm thick combs, casting dams. Combs have one marker lane BCMSMIDI96/2M multiSUB Midi 96, UV tray, Comb block with 12 x 8 sample, 1mm thick combs, casting dams. Combs have two marker lanes BCMSMIDI96/1.5/2M multiSUB Midi 96, UV tray, Comb block with 12 x 8 sample, 1.5mm thick combs, casting dams. Combs have two marker lanes BCMSMIDI96ST multiSUB Midi 96 Stretch, UV tray, Comb block with 12 x 8 sample, 1mm thick combs, casting dams BCMSMIDI96ST1.5 multiSUB Midi 96 Stretch, UV tray, Comb block with 12 x 8 sample, 1.5mm thick combs, casting dams. Combs have one marker lane BCMSMIDI96ST/2M multiSUB Midi 96 Stretch, UV tray, Comb block with 12 x 8 sample, 1mm thick combs, casting dams. Combs have two marker lanes BCMSMIDI96ST/1.5/2M multiSUB Midi 96 Stretch, UV tray, Comb block with 12 x 8 sample, 1.5mm thick combs, casting dams. Combs have two marker lanes BCMSMIDI96STDBL multiSUB Midi 96 Stretch, UV tray, Comb block with 24 x 8 sample, 1mm thick combs, casting dams - Note: run length = 1.8cm.

MS10-UV96 Multi Sub Midi, 96 Well Tray MS10-UV96ST Multi Sub Midi STRETCH, 96 Well Tray Comb Blocks MSMIDI96-8-1-CB MSMIDI 96 Comb 8 sample MC + 1 Marker, 1mm thick MSMIDI96ST-8-1-CB* MSMIDI 96 STRETCH Comb 8 sample MC + 1 Marker, 1mm thick MSMIDI96-8-1.5-CB MSMIDI 96 Comb 8 sample MC + 1 Marker, 1.5mm thick MSMIDI96ST-8-1-CB* MSMIDI 96 STRETCH Comb 8 sample MC + 1 Marker, 1.5mm thick MSMIDI96-8-1/2M-CB MSMIDI 96 Comb 8 sample MC + 2 Marker, 1mm thick MSMIDI96ST-8-1.5/2M-CB* MSMIDI 96 STRETCH Comb 8 sample MC + 2 Marker, 1mm thick MSMIDI96-8-1.5/2M-CB MSMIDI 96 Comb 8 sample MC + 2 Marker, 1.5mm thick MSMIDI96ST-8-1.5/2M-CB* MSMIDI 96 STRETCH Comb 8 sample MC + 2 Marker, 1.5mm thick Combs MSMIDI96-8-1 MSMIDI96-8-1.5

12

MSMIDI 96 Comb 8 sample MC, 1mm thick, 1 marker lane MSMIDI 96 Comb 8 sample MC, 1.5mm thick, 1 marker lane

MSMIDI96-8-1/2M MSMIDI96-8-1.5/2M

MSMIDI 96 Comb 8 sample MC, 1mm thick, 2 marker lanes MSMIDI 96 Comb 8 sample MC, 1.5mm thick 2 marker lanes

*Stretch blocks fit in the Stretch Units only

MULTISUB-4

multiSUB-4 is a compact system capable of running over 1200 samples simultaneously by stacking up to 4 horizontal gels. This achieves high sample throughput while maintaining the advantages of a small and compact unit.

• SEPARATES

A MAXIMUM

1200 15

SAMPLES IN AS LITTLE AS

MINUTES IN

Each multiSUB-4 is supplied with 4 gel trays and 8 combs as standard. Two double-sided comb and three tray length formats, 8x6, 8x12 and 8x18cm are also available.

4 STACkED GEL TRAyS

• DOUBLE-SIDED 1.5MM

THICk

COMBS ALLOw MORE SAMPLE

VOLUME TO BE LOADED INTO

These multichannel-compatible combs and gel plate configurations are compatible with microplates and thermal cycler blocks to ensure rapid loading of DNA minipreps and PCR products by 8-channel pipette.

EACH wELL

• THREE

GEL TRAy OPTIONS

8X6, 8X12 AND 8X18CM (wXL) SIzES FOR AVAILABLE IN

MAXIMUM FLEXIBILITy

An optional Flexicaster allows up to four 8x6, 8x12 and 8x18cm multiSUB-4 gel trays to be cast simultaneously outside the tank.

Technical Specifications GEL DIMENSIONS (W X L) 8 X 6CM, 8 X 12CM, 8 X 18CM UNIT DIMENSIONS

11 X 35 X 16CM

MAX. SAMPLE CAPACITY PER 18CM TRAY

WITH 1CM RUN LENGTH: 306 WITH 2CM RUN LENGTH: 144 WITH 3CM RUN LENGTH: 72

BUFFER VOLUME

200, 400, 600 OR 800ML (FOR 1, 2, 3 OR 4 GEL TRAYS RESP.)

Typical Applications Ideal for rapid checking of very large numbers of samples from PCR and cloning. High throughput Electrophoresis.

casting systems

COMBS AVAILABLE: NO. OF SAMPLES THICKNESSES

• ADjUSTABLE

1, 8, 12, 18 DUOCOMBS 1, 1.5MM

BARRIER wITH ULTRA-SOFT

SILICONE GASkET ENSURES LEAk-PROOF

CASTING

• Mini FLEXICASTER ACCOMMODATES 7X7, 7X10, 10X7 AND 10X10CM GEL TRAyS • Maxi FLEXICASTER ACCOMMODATES 15X7, 15X10 AND 15X15 AND 20X10, 20X15, 20X20 AND 20X25CM GEL TRAyS • Screen Three horizontal flexicasters are available for use with the multiSUB Mini, Midi, Choice, Maxi and Screen gel trays.

Each Flexicaster is entirely versatile and when casting, one end of the gel tray is pushed into position against a fixed wall, while a moveable dam with twin cam-lock design and rubber insert locks into position at the other end to provide a leak proof seal. No further casting accessories are needed other than the gel tray, while adjustable levelling feet and a spirit bubble facilitate even gel-pouring.

Flexicaster for multiSUB-4

FLEXICASTER ACCOMMODATES ALL

TRAy SIzES (GEL CAN BE POURED AT

80°C)

MS26-FC Tray Compatibility MS7-UV7, MS7-UV10

3 of each

MS10-UV7, MS10-UV10, MS15-UV7, 2 of each MS15-UV10, MS15-UV15, MS15-UV17 MS15-UVST20, MS15-UVST25 MS20-UV10, MS15-UV15

1 of each

MS20-UV17, MS15-UV20, MS15-UV25 1 of each MS26-UV16, MS26-UV24, MS26-UV32 1 of each

ORDERING INFORMATION BCMULTISUB4 MULTISUB4EXCAS MSUB4UV6 MSUB4UV12 MSUB4UV18 MSUB4EXCASTER MSUB4-12/1-1 MSUB4-18/8-1

multiSUB-4, Multi-level Gel Chamber, includes 4x 12cm UV Trays, 8x 18/8 Sample 1.5mm Combs (Tape UV Trays to seal) multiSUB-4, as above but with External Caster for 4 gels MSUB4-12/1-1.5 12/1 Sample 1.5mm Combs for multiSUB-4 multiSUB-4 tray 8 x 6cm MSUB4-18/8-1.5 18/8 Sample 1.5mm Combs for multiSUB-4 multiSUB-4 tray 8 x 12cm CAB Replacement cables (red and black) multiSUB-4 tray 8 x 18cm External Caster for multiSUB-4 (4 Trays) MS7/10-FC multiSUB Mini/Midi Flexi Caster 12/1 Sample 1mm Combs for multiSUB-4 MS15/20-FC multiSUB Choice/Maxi Flexi Caster 18/8 Sample 1mm Combs for multiSUB-4 MS26-FC multiSUB Screen Flexi caster

13

HORIZONTAL PACKAGE DEALS

multisub

ORDERING INFORMATION MINIPACK

MINIPACK

MIDIPACK

CHOICEPACK

MAXIPACK

MULTISUB4PACK

SCREENPACK

Mini Horizontal Electrophoresis Package, includes: - BCMSMINIDUO - horizontal unit with 7x7cm and 7x10cm gel trays and combs (Page 6) and NANO300 - 300V, 400mA, 60W mini power supply (Page 49) MIDIPACK Midi Horizontal Electrophoresis Package, includes: - BCMSMIDIDUO - horizontal unit with 10x7cm and 10x10cm gel trays and combs (Page 7) and NANO300 - 300V, 400mA, 60W mini power supply (Page 49) CHOICEPACK Choice Horizontal Electrophoresis Package, includes: - BCMSCHOICETRIO - horizontal unit with 15x7cm, 15x10cm and 15x15cm gel trays and combs (Page 8) and MP-300 - 300V, 700mA, 150W midi power supply (Page 49) MAXIPACK Maxi Horizontal Electrophoresis Package with extra gel tray, includes: - BCMSMAXIDUO - horizontal unit with 20x10cm and 20x20cm gel trays and combs (Page 9) and MP-300 - 300V, 700mA, 150W midi power supply (Page 49) SCREENPACK Screen Horizontal Electrophoresis Package, includes: - BCMSSCREENTRIO - horizontal unit with 26x16cm, 26x24cm and 26x32cm gel trays and combs (Page 10) and MP-500 - 500V, 800mA, 300W maxi power supply (Page 49) MIDI96PACK Midi96 Horizontal Electrophoresis Package, includes: - BCMSMIDI96 - horizontal unit with UV tray and 12x8-sample 1mm comb block (Page 12) and MP-300 - 300V, 700mA, 150W mini power supply (Page 49) MULTISUB4PACK MultiSUB-4 Horizontal Electrophoresis, includes: - BCMULTISUB-4 - horizontal unit with four 8x12cm gel trays and combs (Page 13) and MP-300 - 300V, 700mA, 150W midi power supply (Page 49)

14

runview

NEW

For combs and accessories, see page 8

Base unit with integrated power supply

runVIEW is an innovative new system which can be used to maximise the efficiency of DNA recovery from runSAFE, EtBr and SYBR stained gels by minimising the number of steps involved in post electrophoretic purification. runVIEW consists of an multiSUB CHOICE gel chamber with special bluVIEW lid, containing an orange spectral emission filter within its viewing pane, plus a base unit with integrated power supply and blue LED gel illuminator.

Simple DNA recovery Using runVIEW for gel-free DNA recovery is simple: 1. Cast a gel with two identical rows of wells using one of the matching pairs of 3mm combs supplied. Transfer to the tank, remove the combs, and add buffer just to cover the gel and fill the wells. Load DNA (‘loading tier’).

2. Replace the lid and apply the power. Turn on the blue LED and see the DNA samples migrate in real-time.

3. When the DNA bands of interest enter the second row of wells (‘extraction tier’), stop the power, remove lid and harvest the DNA by pipette.

Once the DNA bands of interest enter the ‘extraction’ tier, simply stop the power supply, remove the lid and harvest the DNA by pipette.

Extraction fan ensures clear viewing area, free from condensation

runVIEW tank with bluVIEW lid

Track DNA without harmful UV

Biocom “run”

Blue light is safe and has none of the detrimental effects of UV transillumination, including mutagenesis which can compromise cloning efficiency, while runVIEW’s capacity to provide real-time visualisation of electrophoresis enables DNA to be tracked as it migrates through the gel. This allows the user to judge precisely when the band of interest is ready for extraction.

series represents

Save on time-consuming gel elution techniques Running DNA into a well containing buffer effectively purifies the DNA of agarose, eliminating the need for timeconsuming gel excision and purification techniques that also result in sample loss. Once harvested, the DNA needs only to be cleaned by a straightforward ethanol precipitation when it is ready for further digestion or ligation.

No expensive commercial gels runVIEW works with standard EtBr, SYBR Green and SYBR Safe gels cast within the 15x7, 15x10 or 15x15cm CHOICE gel trays, and therefore does not require expensive precast gels and accessories.

a safer alternative to the use of UV irradiation and ethidium bromide, both of which are known to have harmful mutagenic effects.

A self-contained system The base unit, which houses the in-built power supply and blue LED gel illuminator, is compact, dual-voltage and portable, and allows electrophoresis, gel visualisation and extraction to be performed at the bench, without the inconvenience of having to transport gels to a darkroom elsewhere within the laboratory.

REAL-TIME GEL ELECTROPHORESIS

Blue LED gel illuminator

Typical Applications Real-time size fractionation and recovery of nucleic acids

runVIEW and runDOC, see pages 16, 17

15

REAL-TIME GEL ELECTROPHORESIS

runview • Power supply integrated within the base unit – adjustable in precise 1V or 1mA increments to a maximum 150V or 300mA constant voltage or current output; timer function to 999 minutes for extended runs • Specialist combs for specialist applications double-sided 1mm preparatory combs (1-/2-sample and 4-/16sample standard) included for nucleic acid recovery, plus four multichannel compatible 20/28-sample combs for rapid screening of nucleic acids from 96-well thermal cycler blocks and microtitre plates. Extra thick 3mm preparatory combs also included for enhanced DNA recovery • Upgrade to real-time existing MSCHOICE users may customise their system by purchasing the base station and bluVIEW lid • Versatile - power supply and gel illuminator may be used with other gels and electrophoresis systems

After gel pouring and sample loading, simply lift and position the runVIEW tank with bluVIEW lid within the base unit

• COMPREHENSIVE –

INCLUDES EVERyTHING*

REqUIRED TO PERFORM

HORIzONTAL REAL-TIME GEL ELECTROPHORESIS

• CONVENIENCE –

COMBINES

THE HIGH RESOLUTION CAPABILITy wITHIN A SINGLE COMPACT BENCH TOP UNIT

• VISIBILITy –

EXTRACTOR

FAN IN LID ELIMINATES

CONDENSATION, TO ALLOw

• OPTIONAL

GEL

DOCUMENTATION SySTEM

FITS DIRECTLy OVER THE BASE UNIT AND GEL TANk FOR IMAGING AT THE END OF THE ELECTROPHORESIS RUN

• VALUE –

COSTS

30-50%

LESS THAN INDIVIDUAL

COMPONENTS; GEL TANk, POwER SUPPLy AND TRANSILLUMINATOR

REAL-TIME OBSERVATION OF

BAND MOVEMENT

runVIEW Viewing Hood

Technical Specifications runVIEW Viewing Base Unit Blue Light Wavelength 470nm Voltage / Resolution 25-150V / 1V Current / Resolution 300mA / 1mA Power 30W Operating Mode Constant Voltage or Current

Timer Safety Device Operating Temperature Dimensions Rated Voltage

1-999 minutes with alarm No load detection Ambient to 40°C 29 x 22 x 8cm 100-240V, 50/60Hz

runVIEW Gel System Gel Dimensions (W x L) runVIEW Lid Design

15 x 7, 15 x 10 & 15 x 15cm VLID1 - bluVIEW lid - bluVIEW lid – Amber (runSAFE & SYBR stains); Built-in extractor fan powered by base unit

Unit Dimensions (W x D x H) Combs

500ml

Comb Thickness

26.5 x 17.5 x 9cm 2 x extra thick 3mm preparatory combs double sided, 4 & 16MC and also 20/28MC. 2 x 1mm doubled sided combs, 4 & 16MC and 4 x double sided combs 20 & 28MC 1mm, 3mm

Buffer volume

ORDERING INFORMATION RVMSCHOICE7 RVMSCHOICE10 RVMSCHOICE15 RVMSCHOICETRIO RVSTATION RVBSBVLID RVLID1 RVLID2 RVHOOD

16

runVIEW System complete with 15 x 7cm gel tray, one set of casting dams and 8 combs runVIEW System complete with 15 x 10cm gel tray, one set of casting dams and 8 combs runVIEW System complete with 15 x 15cm gel tray, one set of casting dams and 8 combs runVIEW System complete with 15x7cm, 15x10 & 15x15 gel trays, one set of casting dams and 8 combs runSTATION complete with RVGELDOC and RVMSCHOICETRIO MS15-1/2-1 Comb 1 & 2 sample, 1mm thick runVIEW Base Station & bluVIEW Lid MS15-4/10MC-1 Comb 4 & 10MC sample, 1mm thick runVIEW bluVIEW lid - Orange (EtBr), optional runVIEW bluVIEW lid - Amber (runSAFE), standard MS15-20/28MC-1 Comb 20 & 28MC sample, 1mm thick MS15-4/2M-3 Comb 4 sample 2 Marker, 3mm thick runVIEW Viewing Hood MS15-6/2M-3 Comb 6 sample 2 Marker, 3mm thick *except chemicals and reagents

GEL DOCUMENTATION

runDOC runDOC is a portable, lightweight gel documentation system designed exclusively for use with runVIEW. runDOC comprises a darkroom hood and a high resolution digital camera to capture images of nucleic acid gels stained with EtBr, SYBR and runSAFE. Its 12.1 megapixel camera with 2GB memory card allows storage of images in RAW and JPEG formats.

Hood is positioned over the runVIEW tank and base unit to create a light-tight environment suitable for image capture

The runDOC darkroom is placed quickly and easily over the runVIEW gel tank located on the runVIEW base unit, which includes an integrated power supply and blue LED gel illuminator. The blue LED illuminator provides the excitation source for the DNA gel located within the tank, and following excitation the light emitted is then visualised using either one of the bluVIEW lid options (supplied and used with the runVIEW gel tank) or the runDOC filter slide. The interchangeable filter slide and bluVIEW filter options offer full flexibility: amber filter for runSAFE, SYBR and green fluorescence; and orange filter for EtBr and red fluorescence. runDOC may be also used like an ordinary gel documentation system - by placing the gel tray directly on the runVIEW base station, which acts as a transilluminator. runDOC is available as a complete system with laptop and software.

NE W

runSAFE

• runSAFE – general purpose stain for DNA ranging from 50bp markers to large super-coiled plasmid • runSAFE-PLUS500 – for DNA larger than 500bp in size;

CAMERA TYPE 5X OPTICAL / 4X DIGITAL ZOOM 12.1MP EFFECTIVE PIXELS 640X480 UP TO 4000X3000 IMAGE SIZE SENSOR 1/1.7” TYPE HIGH SENSITIVITY CMOS RAW, JPEG FILE FORMAT COMPUTER INTERFACE USB 2.0 HI-SPEED (MINI- B-JACK) VIDEO OUT NTSC / PAL 8GB SD MEMORY CARD STORAGE MEDIA LENS 6.1-30.5 MM FOCAL LENGTH MAXIMUM APERTURE F/1.8(W)-F/2.9(T) 15-1/4000S SHUTTER SPEED EMISSION FILTER OPTIONS ORANGE FILTER FOR ETBR; RUNDOC FILTER SLIDE AMBER FILTER FOR SYBR AND RUNSAFE BLUVIEW-ORANGE FOR ETBR; BLUVIEW LID OPTIONS* BLUVIEW-AMBER FOR SYBR AND RUNSAFE DARK ROOM CAMERA HOLDER MATERIAL POLYOXYMETHYLENE (POM) DARKROOM MATERIAL EBONY ACRYLIC DIMENSIONS / WEIGHT 40X34X19.5CM (HXWXD) / 0.8KG POWER RECHARGEABLE LI-ION BATTERY AND PLUG-IN CHARGER OPTIONAL MAINS CABLE CHARGER *ONLY WITH RUNVIEW

runSAFE Range - Less DNA damage, improved cloning efficiency

Plasmid

PCR Products

• SAFE –

ALL FOUR RUNSAFE STAINS HAVE ULTRA-LOw TOXICITy (LC>5000MG/kG) AND LACk CELL PERMEABILITy

10000

1000

• CONVENIENT –

CFU

The runSAFE Range comprises four non-mutagenic stains that produce instant visualisation of DNA bands upon irradiation with blue light or UV in agarose or polyacrylamide gels. Each stain is conveniently supplied in a 6x loading dye which is mixed with 5 parts double-stranded DNA before loading onto an agarose or polyacrylamide gel. The individual colour constituents within each loading dye track electrophoresis run progression, and indicate the extent of band separation and resolution by comigrating with DNA bands of known molecular size, which varies according to the gel percentage. All four stains are non-toxic, safe for the environment and can be disposed of in the regular laboratory waste without using expensive decontamination methods. Each stain is sensitive and binds DNA to detect as little as 0.2ng DNA per band within a gel; while gel imaging is best performed using the amber emission filter found of the bluVIEW lid or runDOC filter slide. The runSAFE Range comprises the following:

Technical Specifications

100

Blue LED System

10 0

15 30 45 Exposure Time (sec)

60

120

Slower migrating species, indicative of a linear or relaxed circular vector, resulting from DNA nicking or strand breaks, are significantly reduced in DNA plasmid mixed with runSAFE and exposed to blue light. The concentration of nicked DNA plasmid increases significantly after 8’ of exposure to EtBR and UV irradiation.

• runSAFE-2000 – for DNA vectors and inserts ranging from 500-2000bp; • runSAFE-500 – for small DNA fragments, PCR products, sequence tracts and primers less than 500bp.

EACH STAIN IS SUPPLIED AS A READy TO USE 6X LOADING DyE; SIMPLy ADD 1 PART STAIN TO 5 PARTS DNA, MIX AND LOAD yOUR GEL

• FAST – NO TIME-CONSUMING POST-STAINING OR DE- STAINING OF GELS IS REqUIRED. • SENSITIVE –

VERy LOw BACkGROUND STAINING OF THE GEL; DETECTS AS LITTLE AS 0.2NG DNA PER BAND

• FLEXIBLE – USED wITH

EACH STAIN MAy BE OR UV LIGHT

BLUE

ORDERING INFORMATION RVGELDOC-F2 Amber Filter for runDOC (runSAFE and SYBR stains) runVIEW Gel Documentation Hood with 12.1MP camera CAMCHARGER Optional Mains Charger Cable for 12.1MP camera runVIEW Gel Documentation Hood with camera, laptop RVSTATION runSTATION complete with RVGELDOC and RVMSCHOICETRIO & 1D Analysis Software RVGELDOC-F1 Orange Filter for runDOC (Ethidium Bromide) RVGDCOMPLETE runVIEW Package including RVGELDOCSYS and RVMSCHOICETRIO runSAFE Description Tracking Dyes Size Range RUNSAFE runSAFE Stain, 1ml Bromophenol Blue, Xylene Cyanol FF, Orange G 50bp – 20Kb RUNSAFE-PLUS500 runSAFE-PLUS500 Stain, 1ml Bromophenol Blue, Xylene Cyanol Blue >500bp RUNSAFE-2000 runSAFE- 2000, 1ml Xylene Cyanol Blue, Orange G 500-2000bp RUNSAFE- 00 runSAFE-500, 1ml Orange G <500bp RVGELDOC RVGELDOCSYS

17

comet assay • FOR SINGLE CELL GEL ELECTROPHORESIS • MINIMISE

EXPOSURE TO

LIGHT AND REDUCE BACkGROUND

• HIGH

DNA

DAMAGE

EFFICIENCy COOLING

FOR ENHANCED RESOLUTION

Background: First introduced in

Overview: Following genotoxic Full Screen Shot of a Comet Profile within the Comet Assay IV Software.

A. The Comet ‘Head’ comprising largely intact genomic DNA. B. The Comet ‘Tail’ of mobile damaged or fragmented DNA.

Technical Specifications SLIDE CAPACITY

10

20

40

UNIT DIMENSIONS (W X L X H)

17X34X9CM

31X34X9CM

33X59X9CM

550ML

1000ML

2100ML

VOLUME RECOMMENDED RUNNING CONDITIONS

25V/300MA FOR 1H

Typical Applications quantifying DNA damage and repair in drug development application.

Reproductive science.

insult, such as ionising radiation, the resultant strand breakage of supercoiled duplex DNA reduces the size of the large genomic DNA from which these strands are separated or drawn out by electrophoresis. The genomic DNA then takes on the appearance of a ‘comet’ as its negatively charged broken ends and fragments migrate towards the anode during electrophoresis.

Method: After exposure to a genotoxic insult cells are suspended within low melting point agarose and embedded within a thin layer of agarose on a microscope slide.

Cellular protein is then removed by lysis in detergent, when DNA is allowed to unwind in alkaline conditions before electrophoresis.

The DNA is electrophoresed, stained and then analysed using software.

COMET assay tanks are available in three slide formats to study single cell gel electrophoresis (SCGE), a technique made popular by drug toxicology and carcinogenesis studies for the detection and quantitation of DNA damage in cells.

Each tank’s robust construction from ebony acrylic ensures that cells remain free of exposure to background light and DNA damage during electrophoresis, while a cooled central platform provides a convenient surface for slide preparation and control of slide temperature during the assay. Following electrophoresis, DNA damage may be measured using Comet Assay scoring software.

CHILLER

1981 to quantify double-stranded DNA breakages in single cells exposed to g-irradiation, the Comet Assay (or SCGE) has since been adapted to analyse specific DNA lesions and repair processes.

Results: Microscopy imaging is used to measure DNA fluorescence upon staining. In DNA damaged cells the resultant image resembles a ‘comet’ with the cellular DNA separated into a head and tail. The head is mainly composed of intact genomic DNA, whereas any fragmented or damaged DNA is concentrated within and towards the tail.

This CHILLER is ready assembled with the thermostat mounted on the refrigerator and supplied with insulated tubing and clips to form a system ready to use. The immersion thermostat incorporates Intelligent Control Optimisation for adaptive High specification intelligent PID temperature control and Chiller includes a powerful integral pump, making the system suitable both for immersing samples and circulating temperature controlled fluid to external devices. It includes a convenient timer function for reaction timing and a variable high temperature alarm setting. The temperature range and heater power are automatically limited according to the liquid type selected. A simple-to-use rotor dial plus two keys provide access to the interactive interface for fast, accurate set-up. 3 year warranty.

Technical Specifications TEMPERATURE RANGE -25 TO 100°C STABILITY (WATER @ 10°C } ± 0.1°C ± 0.1°C UNIFORMITY (WATER @ 10°C) SETTING RESOLUTION 0.1°C DISPLAY 4 DIGIT LED 1 MIN TO 99 HRS 59 MINS TIMER FUNCTION

PUMP FLOW RATE NO. STORED TEMP, VALUES SAFETY OVER-TEMPERATURE HEATER POWER 230 V HEIGHT ABOVE TANK RIM DEPTH BELOW TANK RIM

ORDERING INFORMATION BCOM10 BCOM20 BCOM40

18

Comet Assay Tank for 10 Slides Comet Assay Tank for 20 Slides Comet Assay Tank for 40 Slides

CHILLER

Chiller unit for active slide temperature control

17 L/MIN (MAX.) 3 ADJUSTABLE CUT-OUT 1.3 KW 200MM 135MM

HIGH THROUGHPUT COMET ELECTROPHORESIS SYSTEM

compac-50 Developed in collaboration with the Oxidative Stress Group in the Department of Cancer Studies and Molecular Medicine within the University of Leicester, the COMPAC-50 is a high throughput electrophoresis system, to perform the Comet Assay, otherwise known as Single Cell Gel Electrophoresis.

NEW •

UNIqUE

PATENT PENDING DESIGN

EMPLOyS TwO CARRIERS, EACH CAPABLE OF ACCOMMODATING

SLIDES IN A VERTICAL LAMINAR

A unique patent pending design employs two carriers to hold a total of 50 slides (25 per carrier) vertically. This provides two distinct advantages over conventional Comet Assay systems that utilise a horizontal platform for manual mounting of multiple individual slides. Firstly to produce a highly compact system which saves 75% of Lab space.

Secondly by holding 25 slides in a rack this allows slides to be processed together in one batch saving on handling assay time by up to 90%. Consequently, this is not only beneficial for electrophoresis but also in the lysis, neutralisation, staining and washing steps of the Comet Assay, when each batch of slides may be treated during each step respectively using the four ebony acrylic staining dishes supplied. In addition, the COMPAC-50 benefits from a high performance ceramic cooling base with sliding drawer to accommodate a cool pack, which is frozen before use, to maintain optimal buffer temperature.

25

ORIENTATION

• SLIDE

CARRIERS FACILITATE BATCH-

PROCESSING OF MULTIPLE SLIDES

SIMULTANEOUSLy, THUS ELIMINATING

THE NEED FOR MANUAL HANDLING OF

INDIVIDUAL SLIDES, DURING PRE- AND

POST-ELECTROPHORESIS STEPS OFTHE

COMET ASSAy,

TO MAXIMISE

PROCESSING SPEED

• TEN

STAINING DISHES SUPPLIED FOR

BATCH-TREATMENT OF SLIDES DURING THE LySIS, NEUTRALISATION, STAINING AND wASHING STEPS

• EBONy

ACRyLIC CONSTRUCTION OF

TANk AND STAINING DISHES ENSURES THAT NUCLEI REMAIN FREE OF EXPOSURE TO BACkGROUND LIGHT

AND POTENTIAL

Typical Result Repair of UVB-induced DNA damage in human keratinocytes, using enzyme-modified Comet Assay. HaCaT cells were irradiated with 1 J/cm2 UVB, then allowed to repair in fresh medium and DNA damage analysed at different time points (A) 0 h, (B) 1 h,(C) 6 h, (D) unirradiated (courtesy of Karbaschi, M. University of Leicester, Leicester, UK).

DNA

DAMAGE

COMPACT DESIGN OPTIMISES

ELECTROPHORESIS EFFICIENCy DURING

COMET ASSAy

• ENHANCED

COOLING IS PROVIDED By

A CERAMIC COOLING PLATFORM wITH SPRING LOADED PLATFORM THAT

ACCOMMODATES LOw-COST PRE-

FROzEN COOL PACkS TO MAINTAIN OPTIMAL BUFFER TEMPERATURE

•

COMPAC-50 – Experimental Procedure Overview of a typical Comet Assay procedure. A single-cell suspension of the cells under investigation is mixed with low melting point agarose. The cell/agarose mix is layered on glass microscope slides, pre-coated with agarose, and the agarose allowed to set. The cells are lysed under high pH before washing with pure water. The presence of strand breaks and high pH allows the cellular DNA to unwind. Electrophoresis draws the DNA out of the nucleoid body forming a ‘tail’. The amount of migration (the amount of DNA in the tail versus the head) is proportional to the initial amount of DNA damage. The slides are then drained, neutralised and washed with pure water before drying overnight. Following further washing in pure water the slides are stained, washed and finally scored and analysed, typically using fluorescent microscopy and image analysis software. Advantageously, the use of a slide-holder assembly, which maintains multiple slides in laminar arrangement, also permits batch processing of these slides through preelectrophoresis and post electrophoresis steps, thus eliminating the need for individual manipulation of samples.

• HIGHLy

50

SLIDES MAy BE RUN wITHIN

MP-300 49)

MINUTES USING

SUPPLy

(PAGE

20

POwER

Technical Specifications

Staining Dish

Slide Rack

Rack in Dish Draining

UNIT DIMENSIONS (WXLXH)

26.5 X 15 X 15CM

TOTAL SLIDE CAPACITY

50 SLIDES 25 X 75MM

SLIDE CAPACITY PER RACK

25

VOLUME

550 ML

RECOMMENDED RUNNING CONDITIONS

27V / 450MA FOR 20 MINUTES

RECOMMENDED POWER SUPPLY MP300 300V, 700MA, 150W

ORDERING INFORMATION COMPAC-50

High Throughput Comet System for 50 slides, includes 2x 25 slidecarriers, 4x staining dishes, tank with ceramic cooling platform and cool pack, lid and power cables COMPACK300 COMPAC-50 and MP300 Power Supply 300V, 700mA, 150W COMRAC-25 Vertical slide carrier for 25 slides, pk/1 STAINDISH Ebony acrylic stain dish, pk/1 STAINDISH4X Ebony acrylic stain dish, pk/4

COMPAC-50-PE COMPAC-50-NE CAB MS-NRK NHYBRIDBASIC LMA50

Positive electrode Negative electrode Electrophoresis cables (black and red) Rocking Shaker with 30 x 30cm platform and flat non slip rubber mat (page 64) Incubator only with 2 stainless steel shelves (page 66) Agarose 50g, Low melting point (page 16)

19

• COMPACT

HIGH RESOLUTION

SySTEM FOR CLINICAL ELECTROPHORESIS

• ACCOMMODATES STRIPS GELS UP TO 24X20CM • COMPLETE

AND

RANGE OF

CELLULOSE ACETATE GELS

AND kITS

• DENSITOMETER

SOFTwARE

CELLULOSE ACETATE

clinical AND SCANNER AVAILABLE

Cellulose acetate electrophoresis is an important technique in clinical diagnostics. This new range of cellulose acetate products offers a complete system solution for research and clinical cellulose acetate electrophoresis applications.

The CellasGEL range includes both equipment and consumables to assist in the research and diagnosis of specific disease states.

Cellas Electrophoresis System The ideal tank for standard ‘dry’ membrane and ‘wet’ gel cellulose acetate techniques, the Cellas electrophoresis system is designed and built to our high quality standard to address both routine clinical and research requirements. Two adjustable supports, which can be positioned anywhere within the tank, readily accommodate different lengths of dry cellulose acetate membrane to a maximum 20cm.

NEW Typical Applications qualitative identification and quantification of Hb variants. Finding abnormalities of Hb synthesis like sickle cell disorders, thalassaemias etc.

CellasGEL Wet Membranes Applicators

Unlike dry cellulose acetate strips that are restricted to low volume micro tests, CellasGEL’s greater thickness allows tests to be performed on semi-micro and macro scales using a wide range of specialist applicators. Consequently greater sample volumes may be loaded as larger, but finer bands over a wider front. This reduces sample saturation and aids densitometric band quantitation, thereby improving resolution.

APPS22

APPS4SP

APPSM8

Cellas Boxes

APPS4

APPS6

Paper Wicks

Bridges

Bridges A universal bridge (BDG8.5S) supports each 2.5x14cm and 5.7x14cm CellasGEL during sample application by serving as a convenient loading template for the required applicator. Up to three universal bridges may be then placed into each Cellas tank for electrophoresis.

Densitometer A universal densitometer scanner with software capable of reading 144 tests per minute is available for band quantitation following post-electrophoretic staining.

ORDERING INFORMATION

BCCELLAS GEL2.514250 GEL2.514200 GEL2.514190 GEL5.714500

Horizontal Unit for Cellulose Acetate Electrophoresis 2.5x14 CellasGEL 250 micron 100/pack 2.5x14 CellasGEL 200 micron 100/pack 2.5x14 CellasGEL 190, high resolution, 100/pack 5.7x14 CellasGEL 500, high volume, 25/pack

PART NUMBER

DESCRIPTION

APPS22 APPS4SP APPS4

1x 2-Specimen Semi-micro Applicator 1x 4-Specimen Semi-micro Applicator 1x 4-Specimen Semi-micro Applicator

GEL5.714250 GEL5.714190 GEL2.517200

VOLUME APPLIED / SAMPLE BAND WIDTH

COMPATIBLE STRIP SIZE

PART NUMBER

DESCRIPTION

0.7μl / 7mm 0.9μl / 9mm 1.2μl / 9mm

2.5x14cm 5.7x14cm 5.7x14cm

APPS6 APPM8

1x 6-Specimen Semi-micro Applicator 1x 8-Sample Micro Applicator

CellasGEL Wet Membrane Bridges and Densitometer BDG8.5S 1x 8.5cm bridge for 1x 5.7x14cm or 2x 2.5x14cm CellasGEL strips DENS TurboScan Software Densitometer (excludes computer and scanner) SCAN Flatbed Scanner for TurboScan software

20

5.7x14 CellasGEL 250 micron, 25/pack 5.7x14 CellasGEL 190, high resolution, 25/pack 2.5x17 CellasGEL 200 micron, 25/pack VOLUME APPLIED / SAMPLE BAND WIDTH

COMPATIBLE STRIP SIZE

0.7μl / 7mm 0.3μl / 5mm

5.7x14cm 5.7x14cm

CellasGEL ‘wet’ gels 1. Wet state – unlike dry membranes, CellasGEL is a cellulose acetate film produced in a wet form to facilitate buffer adsorption, but without the entrapment of air bubbles that inhibit electrophoresis

CellasGEL is supplied either as individual packs of 25 or 100 strips or within clinical test kits for the following applications:

• Serum Protein Analysis: Dysproteinaemia; Incipient Gammopathies • Haemoglobin Analysis: Haemoglobinopathies such as Thalassaemias and Sickle Cell Disorders

3. Apply samples to the CellasGEL using the appropriate Applicator, and electrophorese at 200V for 30-9 secs (see Power Supplies, Page 48) 4. Remove the CellasGEL from the tank, and use the required Clinical Test Kit for staining and destaining and clearing 5. Place the CellasGEL on a suitably sized mylar sheet or glass plate and dry in an oven for 10 minutes at 80°C (e.g. NHYBRIDBASIC, page 58)

➤

• Lipoprotein Analysis: Hyperlipidaemias; High-density (HDL), Low-density (LDL) and Very-low-density (VLDL) lipoprotein evaluation

2. Dry surplus buffer from the CellasGEL before securing it to a Bridge located within a preprepared Cellas tank

➤

3. High resolution – samples may be applied to CellasGEL as wider but finer bands, without risk of diffusion, to make band quantitation more reproducible; this is further enhanced by extended migration distances (60-70mm) that improve band separation

• Immunofixation Electrophoresis (IFE): Monoclonal Gammopathies of Undetermined Significance (MGUS); Multiple Myeloma (MM)

1. Equilibrate a CellasGEL for 10 minutes in Electrophoresis buffer using an agitating platform (e.g. 3D Shaker, page 56)

➤

2. Greater thickness – CellasGEL’s greater thickness (190- 500μm) compared to dry membranes (160-190μm) allows application of larger sample volumes to enhance detection of poor quality specimens low in protein content

Using CellasGEL

➤

4. Amphiphilic – CellasGEL’s lipophilic and hydrophilic properties make it the perfect separation medium for many different biological molecules, ranging from lipoproteins to haemoglobins

➤

CellasGEL ‘wet’ cellulose acetate gel strips are ready to use and overcome many of limitations of traditional ‘dry’ cellulose acetate membranes. CellasGEL’s advantages over dry cellulose acetate membranes are as follows:

6. Quantify bands using Scanner and Densitometer Software

ORDERING INFORMATION, CELLASGEL APPLICATIONS PACkAGES

EqUIPPED wITH BRIDGES, APPLICATOR AND CLINICAL TEST kIT

CLINICAL TEST KIT

CODE

DESCRIPTION

KITCU

CellasKIT: 1-patient test for serum and concentrated urine IFE 2x semi-micro tests (1x serum and 1x urine) for 1 patient performed on 6x CellasGEL strips (2.5x14cm; 190μm) on 3x bridges in 1x CELLAS* Kit content, sufficient for 5 patients (10x semi-micro tests): 30 CellasGEL strips & TGS buffer; Coomassie stain, clearing & saline solutions; volumetric distributors & antisera (anti- IgG, IgA, IgM, Ig & Ig); blotting paper & mylar film. Excludes: Destain.

KITI2432

CellasKIT: 6-patient test for serum IFE 6x semi-micro or 8x micro tests (1/patient) on 6x CellasGEL strips (5.7x14 cm; 190μm), performed on 6x bridges in 2x CELLAS units**.

Kit content, sufficient for 24 patients (semi-micro) & 32 (micro): 24 CellasGEL strips & Tris- Hippurate buffer; Amidoblack stain, saline & clearing solutions; volumetric distributors & antisera (anti-IgG, IgA, IgM, Ig & Ig); blotting paper & mylar film. Excludes: Destain

KITSP100200

KITSP150200

KITHG100

KITLP100

CellasKIT: serum proteins 4x semi-micro or 8x micro tests for each CellasGEL strip (5.7x14cm; 200μm); 3 strips / CELLAS* performed on 3x bridges.

Kit content, sufficient for 100x semi-micro or 200x micro tests: 25 CellasGEL strips & Tris-Hippurate buffer; Ponceau S stain, destain & clearing solutions; blotting paper & mylar film CellasKIT: serum proteins (high resolution) 6x semi-micro or 8x micro tests for each CellasGEL strip (5.7x14cm; 190μm); 3 strips / CELLAS* performed on 3x bridges.

Kit content, sufficient for 150x semi-micro or 200x micro tests: 25 CellasGEL strips & TGS buffer; Coomassie stain, citric acid & clearing solutions; blotting paper & mylar film. Excludes: Destain CellasKIT: haemoglobin 4x semi-micro tests for each CellasGEL strip (5.7x14cm; 250μm); 3 strips / CELLAS* performed on 3x bridges.

Kit content, sufficient for 100x semi-micro tests: 25 CellasGEL strips & Tris-Glycine buffer; Ponceau S stain, destain & clearing solutions; blotting paper & mylar film.

CellasKIT: lipoproteins 4x semi-micro tests for each CellasGEL strip (5.7x14cm; 250μm); 3 strips / CELLAS* performed on 3x bridges Kit content, sufficient for 100x semi-micro tests: 25 Cellogel strips & Tris-Hippurate buffer; Sudan Black stain & clearing solution; blotting paper & mylar film

ACCESSORIES REQUIRED BRIDGES APPLICATOR

PACKAGE (KIT + ACCS) CODE

DIAGNOSTIC APPLICATION

1x APPS22 (semi-micro)

3x BDG8.5S

KITSPHR-ABS Includes: 1x KITSP150200 1x APPS4, 3x BDG8.5S

MGUS, MM

1x APPS6 (semi-micro)

6x BDG8.5S

KITI2432-ABS Includes: 1x KITI2432, 1x APPS6, 6x BDG8.5S

1x APPM8 (micro)

6x BDG8.5S

1x APPS4SP (semi-micro)

3x BDG8.5S

1x APPM8 (micro)

3x BDG8.5S

1x APPS6 (semi-micro)

3x BDG8.5S

1x APPM8 (micro)

3x BDG8.5S

KITSPHR-ABM Includes: 1x KITSP150200 1x APPM8, 3x CSLBDG8.5S

1x APPS4 (semi-micro)

3x BDG8.5S

KITHG100-ABS Includes: 1x KITHG100, 1x CSLAPPS4 3x BDG8.5S

1x APPS4 (semi-micro)

3x BDG8.5S

KITLP100-ABS Includes: 1x KITLP100 1x APPS4 3x BDG8.5S

MGUS, MM KITI2432-ABM Includes: 1x KITI2432, 1x APP8M 6x CSLBDG8.5S KITSP-ABS Includes: 1x KITSP100200 1x APPS4, 3x BDG8.5S KITSP-ABM Includes: 1x KITSP100200 1x APPM8, 3x BDG8.5S KITSPHR-ABS Includes: 1x KITSP150200 1x APPS4, 3x BDG8.5S

Dysproteinaemia; Albumin, Alpha-1, Alpha-2, Transferrin, C3 & Gamma Globulin Quantitation

Incipient Gammopathies

Haemoglobinopathies

Hyper-lipidaemias

21

• AVAILABLE IN POPULAR 25X76, 60X76 AND 94X76MM SIzES • ROBUST MyLAR FILM BACkING - ALLOwS THEIR USE ON AUTOMATED PLATFORMS

• UNLIMITED

SHELF LIFE

• COMPATIBILITy -

wITH MANUAL AND AUTOMATED SySTEMS SUCH AS HELENA LABORATORIES, INTERLAB AND SELEO

• 30X76 & 60X76MM MEMBRANES FOR GENIO INTERLAB SMALL AND

STANDARD SIzED AUTOMATED PLATFORMS

• 70X62MM

PUNCHED MEMBRANES FOR INTERLAB MICROTECH 648 AUTOMATED PLATFORM

• 70X60MM

PUNCHED MEMBRANES FOR THE SELEO ADALyA, EXPRIME AND GIANT AUTOMATED PLATFORMS

• OTHER

SIzES AVAILABLE FOR

OLDER AUTOMATED SySTEMS

STILL IN USE

• DENSITOMETER

SOFTwARE AND

SCANNER COMPATIBLE

CELLASMEM DRY MEMBRANES

clinical Although CellasGEL cellulose acetate gels have a number of advantages over traditional dry cellulose acetate membranes, many manual systems and modern robotic platforms still use dry cellulose acetate membranes, which are usually supported on a plastic backing. Consequently, CellasMEM, a range of dry cellulose acetate membranes supported on a Mylar film has been developed to address this demand.

Each CellasMEM dry cellulose acetate membrane is available in 25x76mm (MEM257650), 60x76mm (MEM607625) and 94x76mm (MEM947625) plate sizes, and is compatible with the manual and automated platforms of many leading and emerging brands within the clinical electrophoresis market, including: Helena Laboratories, Interlab and Seleo.

CellasMEM is supplied either as individual packs of 25 or 50 strips to perform manual assays for Serum Protein (Dysproteinaemia; Incipient Gammopathies) and Haemoglobin Analyses (Haemoglobinopathies such as Thalassaemias and Sickle Cell Disorders).

Applicators The CellasMEM MEMAPPM8 is an 8-sample micro applicator designed for use with 60x76mm (MEM607625) and 25x76mm (MEM257650) CellasMEM dry plates. The applicator dispenses each sample deposit as a thin band 5mm wide that is equivalent to 0.25μl in volume; and may load either one 60x76mm strip or two 25x76mm strips (4 samples per strip) at a time. By loading each sample as a tighter, but finer, band over a wider front, the sample diffusion and saturation typical of standard syringe loading methods is significantly reduced, resulting in improved band quantitation. A MEMAPPS6 semi-micro applicator is also available to load 6 samples, each sample deposit 7mm wide and corresponding to 0.5μl in volume.

Bridges Adjustable bridges within the CELLAS tank render specialist bridges unnecessary. Both CELLAS bridges may be positioned either side of the central buffer partition within the tank to produce the 76mm gap necessary to support

ORDERING INFORMATION

Helena-type membranes, while the 24cm width of the tank accommodates either three 60x76mm or six 25x76mm CellasMEM membranes per run. A dedicated bridge adaptor (MEMBA) is available for those users of different cellulose acetate electrophoresis tanks that do not have adjustable bridges.

Paper Wicks Supplied in packs of 100 and available in 190x60mm and 220x40mm (WxL) sizes, CellasMEM disposable paper wicks may be used respectively with standard Cellas MEM dry plates and CellasMEM dry plates for Helena applications. To set up the Cellas tank for use with dry plates, simply insert each paper wick lengthwise within the tank pre-filled with buffer, so that the buffer will become absorbed. Once absorbed, fold over the top of each wick to make a support bridge, ensuring the bottom edge of the wick is immersed within buffer and in contact with the bottom of the tank, while the top edge rests along the adjustable bridge. Repeat for the other bridge.

CellasMEM Membranes CellasMEM membrane plates are available in many different sizes and quantities ranging from the market-leading Helena Titan 3 manual system to punched dry membrane plates compatible with the strip-holders of automated systems from Genio Interlab and SELEO. Also listed are CellasMEM membranes for older systems (some of which are obsolete) that are still in use today.

CellasMEM Package Deals Package deals provide a quick and convenient solution for those users wishing to perform the more popular Helenatype applications. The most basic kit includes 25x76 and 60x76mm CellasMEM membranes, 8-sample micro applicator, and paper wicks, while the complete version also contains a Cellas tank and new NANO500 power supply.

CellasMEM Dry Membrane Applicators MEMAPPM8 CellasMEM 8-sample micro applicator MEMAPPS6 CellasMEM 6-sample semi-micro applicator Paper Wicks MEMWICK CellasMEM paper wicks 190x60mm, pack of 100 MEMWICKH CellasMEM paper wicks 220x40mm, pack of 100; suitable for Helenatype cellulose applications with MEM257650, MEM607625, MEM577625, MEM947625, MEM9413525 membranes

CellasMEM Package Deals MEMHKIT CellasMEM Helena-Type Kit, includes 25x76mm (MEM257650) & 60x76mm (MEM607625) membranes; MEMAPPM8 8-sample micro applicator & MEMWICKH 220x40mm paper wicks MEMHCOMP CellasMEM Helena-Type Workstation, includes MEMHKIT, CELLAS tanks & NANO500 (500V, 400mA, 120W) power supply (page 53 )

Code

Code

Size (WxL)

Pack Size

CellasMEM manual assay membrane MEM257650 25x76mm 50/pack MEM607625 60x76mm 25/pack MEM577625 57x76mm 25/pack MEM947625 94x76mm 25/pack MEM9413525 94x135mm 25/pack MEM7660P25 76x60mm, punched 25/pack

22

Compatible Manual System / Automated Platform Helena Titan 3 system Helena Titan 3 system Helena Titan 3 system Helena Titan 3 system Helena Titan 3 system SELEO AdaLya 24, Selvet 24, Thera 72, Exprime, Giant

Size (WxL)

Pack Size

CellasMEM assay membrane MEM307625 30x76mm 25/pack MEM607624 60x76mm 24/pack MEM7662P25 76x62mm, punched 25/pack MEM7413625 74x136mm 25/pack MEM7822725 78x227mm 25/pack MEM8012525 80x125mm 25/pack MEM2508025 280x80mm 25/pack MEM8022525 80x225mm 25/pack MEM762325 76x23mm 25/pack MEM678930 67x89mm 30/pack MEM7618025 76x180mm 25/pack MEM7621025 76x210mm 25/pack

Compatible Manual System / Automated Platform Genio Interlab - small Genio Interlab - standard Interlab 648 ISO, 648 PC SAE - NT SAE 500/600 Diafero Standard Diafero Extra Cliniphor Saechem Smart Pragma Megaphore

UV STERILISATION

safety cabinets These UV Sterilisation Cabinets provide a convenient area for setting up PCR reactions in a nucleic acid free environment thus limiting PCR reaction contamination.

• INACTIVATES NUCLEIC 5 TO 30 MINUTES

Acting effectively as a low cost alternative to a clean room, the powerful UV lights on each cabinet denatures nucleic acids in 5 to 30 minutes making them unsuitable for amplification. The cabinets incorporate safety features to prevent user-exposure to UV light.

• DOORS

ACIDS IN

FITTED wITH SAFETy

SwITCHES

• COMPLETE wITH FOUR POwERFUL, TIMER CONTROLLED UV BULBS

The UV lights are timer controlled and there are safety switches on the cabinet doors which power off the UV lights when opened. The units’ white light provide excellent visibility when working within the cabinet.

• EFFICIENT

DECONTAMINATION

OF THE COMPLETE wORk

SURFACE

Constructed from 10mm acrylic, the cabinets also act as efficient shields from beta radioactive emissions and can therefore be safely used with isotopes such as 32P.

• SUITABLE

32

FOR wORk wITH

P

Technical Specifications Mini Cabinet

UV LIGHTS Shown with SpillTray

Two models are available, Maxi as shown above, and a new Mini Cabinet for limited budget and bench space. An optional HEPA Filter extraction system is available, suitable for removal of VOC’s/Solvent fumes and particulate – please call.

NEW

MAXI MINI

FOUR X 15 WATT FOUR X 15 WATT

WHITE LIGHT MAXI MINI

15 WATT 15 WATT

DIMENSIONS MAXI (HXWXD) MINI

660 X 560 X 420MM 400 X 560 X 350MM

WEIGHT

19 KG 12 KG

MAXI MINI

Safety SpillTrays and Liners of size 68 x 54cm, provide convenient containment of spillage (not included with cabinet).

gel-X

disposable

gel excision tips Gel-X Excision Tips offer a convenient and efficient one handed method of removing bands using a simple and rapid two-step process. The tips, in two sizes, 4.0 x 1mm and 6.5 x 1mm, cut directly into agarose or acrylamide gels, so eliminating cross contamination between samples.

Alternative methods which require multiple steps including washing or rinsing are slow and tedious. Gel-X is a safe and efficient one handed operation, with a push button gel and tip release, providing researchers with uniform extractions. Tips fit standard 1000µl pipettes and are available in bags and racks.

• EASILy

REMOVE BANDS FROM

AGAROSE GELS

• TwO

TIP SIzES

• ONE

HANDED OPERATION

• REGULAR

PIPETTING ACTION

• ELIMINATES

CROSS

CONTAMINATION

• FIT STANDARD 1000µL PIPETTES Typical Applications Ideal for quick checks of samples from PCR and cloning

ORDERING INFORMATION

UVCAB UV PCR Cabinet, Maxi (without Safety SpillTray) UVCABMINI UV PCR Cabinet, Mini (without Safety SpillTray) UVTUBE Replacement UV Fluorescent Tube WHTUBE Replacement White Fluorescent Tube Gel Excision Tips GELX4 Rectangular Tips - 6.5mm x 1mm, bag/250 GELX4 RACK Rectangular Tips - 6.5mm x 1mm, 5x racks of 48 For 110 V AC, please add ‘-$’ as a suffix to the appropriate product code

BC1006 BC1046 BC1116

GELX6.5 GELX6.5RACK

Safety SpillTray, White Safety SpillTray, Yellow Safety Tray Liners, APET, pk/25

Rectangular Tips - 4.0mm x 1mm, bag/250 Rectangular Tips - 4.0mm x 1mm, 5x racks of 48

23

• LOw EEO

GENERAL PURPOSE

AGAROSE RESOLVES

25kB

IN SIzE

100BP

• LOw MELTING POINT –

TO

FOR

NUCLEIC ACID RECOVERy

–

TyPICALLy RESOLVES FRAGMENTS

200BP

TO

25kB

• HIGH RESOLUTION – PCR GRADE – HIGH RESOLUTION,

LOw

BACkGROUND FOR ANALySIS OF FRAGMENTS,

20-800BP

HIGH GRADE AGAROSE

chemicals and reagents CleverGEL is a new environmentally friendly agarose suitable for routine analysis of nucleic acids using standard electrophoretic procedures.

CleverGEL is manufactured by a process which excludes organic solvents harmful to marine life, making it far kinder to the environment than conventional agarose.

A low EEO (electroendoosmotic) flow minimises diffusion so that even the smallest of nucleic acid fragments remains sharp and tightly resolved, while a high gel strength aids handling and maintains compatibility with blotting techniques.

CleverGEL is now available in a low melting point form for nucleic acid recovery and enzymatic applications, as well as in a high resolution PCRgrade form to resolve very small nucleic acid fragments 20-800bp in size.

Technical Specifications CAS Gelling Point* Melting Point* Solubility Moisture Gel Strength Nuclease & Protease Free *For a 1.5% [w/v] gel

• CONVENIENT - EACH TABLET CONSTITUTES 0.5G OF AGAROSE • NO wEIGHING NECESSARy • RAPID -

SIMPLy ADD THE

REqUIRED NUMBER OF TABLETS TO BUFFER

• FAST DISSOLVING • CONSISTENT GEL PERCENTAGE

Low EEO 9012-36-6 36°C±1.5°C 88°C±1.5°C Clear, colourless @ 1% [w/v] solution ≤10% >1200 g/cm2 (1% [w/v] Gel) Yes

Low Melting Point High Resolution 39346-81-1 39346-81-1 26-30°C ≤33°C ≤65°C ≤70°C Clear, colourless @ 2% [w/v] solution ≤10% ≤10% >200 g/cm2 (1% [w/v] Gel) ≥750 g/cm2 (1.5% [w/v] Gel) Yes Yes

Instant Agarose Tablets

Environmentally friendly CleverGEL agarose is now available in tablet form for routine analysis of nucleic acids using standard electrophoretic procedures. Supplied as 200 tablets in total, each tablet contains 0.5g of CleverGEL agarose which eliminates the need to weigh out loose agarose powder. Because each tablet is fast-dissolving all that is required is to add the desired number of tablets to the buffer, heat, dissolve and then prepare the gel as normal. CleverGEL agarose tablets are provided in blister packs of 10, rather than bulky bottles, for extra convenience and storage.

• ENVIRONMENTALLy FRIENDLy LOw EEO AGAROSE Typical Applications For horizontal agarose gels resolving nucleic acids 0.1-10kb in size. For industrial and academic laboratories adhering to rigorous standard operating procedures and protocols

ORDERING INFORMATION

Low Melting Point General Purpose LMA5 Agarose 5g, LMP AG5 Agarose 5g, Low EEO LMA50 Agarose 50g, LMP AG100 Agarose 100g, Low EEO LMA100 Agarose 100g, LMP AG500 Agarose 500g, Low EEO High Resolution PCR-grade AG1000 Agarose 1000g, Low EEO (2x500g bottles) HRA100 Agarose 100g, HR AG2000 Agarose 2000g, Low EEO (4x500g) HRA500 Agarose 500g, HR AG5000 Agarose 5000g, Low EEO (10x500g) AG10KG Agarose 10Kg, Low EEO (20x500g) Agarose Tablets AGTAB Agarose 100g, Low EEO (200x0.5g tablets, supplied as 20 blister packs of 10x0.5g tablets)

24

NE W

Technical Specifications CAS EEO Gelling Point* Melting Point* Solubility Moisture Gel Strength Nuclease & Protease Free *For a 1.5% [w/v] gel

9012-36-6 <0.13 36°C±1.5°C 88°C±1.5°C Clear, colourless @ 1% [w/v] solution ≤10% >1200 g/cm2 (1% [w/v] Gel) Yes

DNA LADDERS

These ready-to-use DNA Size Markers are designed for horizontal gel electrophoresis use. The 100 bp ladder and 1 kb ladder represent the two most popular markers used for DNA fragment size determination while the new Low, High and Broad range markers offer the widest sizing range from 125 bp to 10000 bp. All markers are formulated to run accurately and to provide crisp band patterns. They require no additional preparation and contain Bromophenol Blue for ease of use. All markers are supplied at a concentration of 50µg/500µl (0.1µg/µl).

100 bp Ladder DNA Marker (100-3,000 bp)

The 100 bp DNA Ladder contains 11 discrete DNA fragments ranging in size from 100 bp to 3,000 bp.

This marker is ideal for the size determination of PCR products.

bp 3,000

ng/10 µl 108

2,000

147

1,500

116

1,000

77

800 700 600 500 400

62 54 46 150 60

300

56

200

60

100

60

1 Kb Ladder DNA Marker

(300-10,000 bp)

The 1 kb DNA Ladder contains 13 discrete DNA fragments ranging in size from 300 bp to 10,000 bp.

This marker is ideal for the size determination of digested DNA.

bp

ng/10 µl

10,000 8,000 6,000 5,000 4,000

80 80 80 80 80

3,000 2,500

120 123

2,000

81

1,500

73

1,000

41

700

57

500

61

300

44

• READy • CRISP

BAND PATTERNS

• INCLUDES BROMOPHENOL BLUE FOR EASE OF USE

• STABLE AT ROOM TEMPERATURE

Broad Range Marker (250-8,000 bp)

The Broad Range marker has 11 discrete fragments: 75 ng/10 µl of 8000 bp, 75 ng/10 µl of 6000 bp, 75 ng/10 µl of 5000 bp, 65 ng/10 µl of 4000 bp, 90 ng/10 µl of 3000 bp, 165 ng/10 µl of 2000 bp, 60 ng/10 µl of 1500 bp, 210 ng/10 µl of 1000 bp, 50 ng/10 µl of 750 bp, 60 ng/10 µl of 500 bp, 75 ng/10 µl of 250 bp

bp

ng/10 µl

8,000 6,000 5,000 4,000 3,000 2,000

75 75 75 65 90 165

1,500

60

1,000 750

210 50

500

60

250

75

High Range Marker

bp

ng/10 µl

10,000 8,000 6,000 5,000 4,000

100 100 100 100 100

3,000

230

2,000

70

(1,000-10,000 bp)

The High Range marker has 8 discrete fragments: 100 ng/10 µl of 10000 bp, 100 ng/10 µl of 8000 bp, 100 ng/10 µl of 6000 bp, 100 ng/10 µl of 5000 bp, 100 ng/10 µl of 4000 bp, 230 ng/10 µl of 3000 bp, 70 ng/10 µl of 2000 bp and 200 ng/10 µl of 1000 bp.

Low Range Marker

bp

ng/10 µl

3,000 2,000 1,500

120 220 65

1,000

235

750

70

500

80

250

105

125

105

(125-3,000 bp)

1,000

The Low Range marker has 9 discrete fragments: 120 ng/10 µl of 3000 bp, 220 ng/10 µl of 2000 bp, 65 ng/10 µl of 1500 bp, 235 ng of 1000 bp, 70 ng/10 µl of 750 bp, 80 ng/10 µl of 500 bp, 105 ng/10 µl of 250 bp, and 105 ng/10 µl of 125 bp.

200

Technical Specifications Product Code Size Range Number of bands Reference bands Package concentration Storage Recommended loading vol.

Tracking dyes Source

TO USE

Typical Applications

MDNA-100BPH 100-3000bp 12 500, 1500bp 54µg/500µl vial

MDNA-100BP MDNA-1KB MDNA-50BP MDNA-BR 100-1500bp 250bp-10Kb 50-1500bp 100bp-10Kb 11 13 17 19 500, 1500bp 1Kb, 3Kb 200, 500bp 500bp, 1.5 & 3Kb 50µg/500µl vial 50µg/500µl vial 56µg/500µl vial 86µg/500µl vial 6 months at 25°C, 12 months at 4°C & 24 months at - 20°C 5µl/well 5µl/well 5µl/well 5µl/well 5µl/well Orange G, Xylene Cyanol FF, Bromophenol Blue Proprietary plasmids and PCR fragments phenol-extracted following restriction digestion and dissolved in 10mM Tris-HCl (pH 8.0) and 10mM EDTA

MDNA-HR 250bp-25Kb 14 1Kb, 3Kb 52µg/500µl vial

Size determination of DNA and RNA bands on gels

5µl/well

NE W

Powdered and Liquid Buffers

TBE and TAE are available in both powder and liquid form. Both TBE and TAE are widely used to separate nucleic acids by horizontal gel electrophoresis, and occasionally in vertical polyacrylamide gels. TBE has a higher buffering capacity than TAE, which is used for faster separations of linear double-stranded DNA. Dry TBE is supplied in packs of 10 powder sachets to maintain shelf life. Each buffer sachet may be opened as required and reconstituted in distilled water to make 1 litre of working solution.

Technical Specifications TAE

EACH 50X SOLUTION CONTAINS: TRIS-BASE (2.0M FINAL STOCK CONCENTRATION); GLACIAL ACETIC ACID (1.0M); EDTA, PH 8.0 (0.05M); FOLLOWED BY DISTILLED WATER TO 1L

TBE

EACH 10X SOLUTION / POWDER CONTAINS: TRIS-BASE (0.112M FINAL STOCK CONCENTRATION); BORIC ACID (0.112M); EDTA, PH 8.0 (0.02M); FOLLOWED BY DISTILLED WATER TO 1L

Buffers are also provided as ready-made 50x TAE and 10x TBE solutions in 1 and 5 litre volumes. These are ideal for laboratories running horizontal nucleic acid gels on a daily basis that require off-the-shelf working stock solutions.

ORDERING INFORMATION DNA Ladders MDNA-100BPH 100bp DNA ladder, 100-3000bp, 1x500µl vial MDNA-100BP 100bp DNA ladder, 100-1500bp, 1x500µl vial MDNA-1KB 1Kb DNA ladder, 250bp-10Kb, 1x500µl vial Powdered and Liquid Buffers TBEP Powdered Tris-Borate-EDTA Running Buffer – 10 sachets (1 litre / pack) TBE10X1L Buffer Tris-Borate-EDTA Running Buffer, 10 x 1L TBE10X5 Buffer Tris-Borate-EDTA Running Buffer, 10 x 5L

MDNA-50BP MDNA-BR MDNA-HR

50bp DNA ladder, 50-1500bp, 1x500µl vial Broad Range DNA ladder, 100bp-10Kb, 1x 500µl vial High Range DNA ladder, 250bp-25Kb, 1x500µl vial

TAE50X1L TAE50X5L

Buffer Tris-Acetate-EDTA Running Buffer, 50 x 1L Buffer Tris-Acetate-EDTA Running Buffer, 50 x 5L

25

omnipage setting a new standard in vertical electrophoresis

INJECTION MOULDED MINI UNIT - durable, leak-proof environment for complete safety and long life COMPATIBLE with all major brands of precast gels RAPID GEL CASTING AND LOADING ensures trouble free gel set-up VERSATILE - interchangeable modules for PAGE and 2-D electrophoresis as well as electroblotting using a single universal buffer tank

PRESSURE

CLAMPING FRAME

A unique single piece pressure frame offers the option of rapid single screw per side or new screw free, sliding gates set up (see page 28). Even pressure and buffer contact across the plates results in even sample migration and efficient heat removal from the gel. Glass plates are 2mm thick to reduce breakage.

RUN

BUFFERS, REAGENTS EFFECTIVE

BUFFER COOLING

Complete system for PAGE, blotting and 2D capillary

26

4

GELS AT A TIME

AND

CONSUMABLES

All Biocom chemicals and reagents for electrophoresis and molecular biology techniques are manufactured to the highest standards. They are stringently tested to ensure they are completely free of impurities.

A simple to use cooling pack system ensures enhanced resolution without costly and time consuming additional equipment. No chiller, tap or obstructing connecting leads are required. The cooling pack is simply pre-chilled in a freezer and placed in the gel tank. Additionally, the use of cooling packs reduces buffer volume.

dedicated SySTEMS...

UP TO

While most vertical gel units can run only one or two gels, omniPAGE Mini units can run one, two or up to four gels (TETRAD10 options) at any time using a triple glass plate sandwich.

in

mini System for PAGE and blotting

AnD

maxi System for PAGE and 2D capillary

All systems available in Mini, Mini Wide and Maxi WAVE formats

omniPAGE maxi WAVE, 20x20cm

electroblotting module

omniPAGE mini, 10x10cm

2-D module

casting base

electroblotting module

2-D module

casting base

OMNI - om-ni- all, everything

Corrosion resistant, 99.99% pure platinum electrodes and gold plated terminals - easily replaced, ensuring minimum repair times

Retractable connectors compatible with most major power supplies

The omniPAGE range of Vertical Gel Units combines ease of use with high resolution separations. Four sizes, Mini 10 x 10cm, Mini Wide 20 x 10cm, WAVE Maxi 20 x 20cm and Maxi Plus 20 x 30cm,

share a host of common features including a guaranteed leak proof seal required for trouble free, rapid and uncomplicated gel casting. The built in gel running module eliminates time consuming transfer of glass plates during casting, a process which can cause gel damage and misalignment. Glass plates with permanently bonded spacers guarantee perfect spacer alignment. The glass plate sandwich is simply inserted between pressure bars and sealed using and easy to use sliding gates or WAVE Maxi Clamp system. This ensures fast set up times while even pressure bars and ultra soft seals guarantee leak proof casting. Once the gel has polymerised, the gel running module is simply inserted into the gel tank for electrophoresis.

combs & SPACERS

• combs and spacers are injection moulded for consistency and ‘clean’ well formation • four thicknesses, colour coded. Black and white combs recommended for high resolution publication quality data; red and blue to scale-up volumes for preparatory techniques

modules INTERCHANGEABLE

black: white: red: blue:

0.75mm for ultra resolved bands 1mm supplied as standard 1.5mm for maximising sample volume 2mm for maximising sample volume

• reversible combs also serve as loading indicators to aid pipette-well alignment, preventing sample loading errors

All units are CE marked and are in conformity with the relevant CE Safety Directives: 73/23/EEC: LOW VOLTAGE DIRECTIVE: IEC 61010-1:2001. EN 61010-1:2001; 89/336/EEC EMC DIRECTIVE: EN55011 Emissions, EN50082-1 Immunity.

s e t t i n g a n e w sta n da r d i n v e r t i c a l e l e ct r o p h o r e s i s

• INjECTION

MOULDED CONSTRUCTION

• COMPATIBLE wITH ALL 8 X 10 AND 10 X 10CM

MINI VERTICAL

omnipage MINI TETRAD

PRECAST GELS

A 4-gel Mini TETRAD system is created by simply introducing additional plates with spacers and appropriate combs. TETRAD10 is supplied with a casting base and external casting upstand to allow gels to be prepared in advance, ready for the next run.

• RAPID

GEL CASTING AND LOADING

• LOw

BUFFER VOLUMES

• RAPID

SET UP COOLING

• RUN UP TO FOUR GELS IN TETRAD MODEL, SEE PAGE 38

For TETRAD systems, see page 38

casting base gel release tool

Technical Specifications PLATE DIMENSIONS, 10 X 10CM GEL DIMENSIONS, 7.5 X 8CM (W X L) UNIT DIMENSIONS

19 X 13 X 15CM (W X D X H)

MAX. SAMPLE CAPACITY

80 SAMPLES, 20 SAMPLES PER GEL

BUFFER VOLUME

MIN 250ML;

MAX 1200ML

COMBS AVAILABLE : NO. OF SAMPLES 1, 5, 8MC, 9, 10, 12, 16MC, 20 THICKNESSES 0.75, 1, 1.5, 2MM

Typical Applications Mini SDS PAGE, Native PAGE, Gradient, Second dimension and Nucleic acid separations

The preferred unit for routine mini protein electrophoresis, omniPAGE mini is constructed using the latest injection moulding manufacturing techniques. This gives a high quality, low cost product with unsurpassed finish, durability and strength. The unit incorporates a sealing system which is compatible with all major types of 8 x 10cm and 10 x 10cm precast gel.

Gel casting and running utilise the same module, no transfer of glass plates during gel casting is necessary. The module contains the option of sliding clamps or just one screw per clamping bar side (both supplied), allowing very rapid set up of both hand cast and precast gels. Ultra soft silicone seals and pressure bars which surround the glass plates guarantee leak proof gel casting. 2mm thick glass plates minimise breakage and have bonded spacers for convenience.

Accessory electroblotting and tube gel modules are available which use the same outer tank and lid.

For extended cooling of the buffer chamber a factory fitted cooling coil option is available. This allows the connection to a recirculating chiller. If required, please add ‘-CC’ to the appropriate product code.

interchangeable MODULES omniPAGE Mini blotting and capillary tube gel modules may be used with the same tank and lid to adapt an Mini Standard or Tetrad system for fast, high-quality electroblotting of mini gels and for reproducible 2-D electrophoresis, respectively. These modules are available as part of stand-alone options or as fully integrated electrophoresis systems.

ORDERING INFORMATION BCVS10D BCVS10DSYS BCVS10DSYS-CU BCVS10PRE VS10DCAST VS10DCASTM VS10EXCASTER VS10DIRM1 VS10ICB VS10NG VS10PG VS10NGS0.75 VS10PGS0.75 VS10NGS1

28

omniPAGE Mini, 10 x 10cm includes Glass Plates with bonded 1mm thick spacers, 2x 12 sample combs, cooling pack, dummy plate omniPAGE Mini, 10 x 10cm incl. Glass Plates with bonded 1mm thick spacers, 2x 12 sample combs, cooling pack, dummy plate and casting base omniPAGE Mini, 10 x 10cm incl. Glass Plates with bonded 1mm thick spacers, 2x 12 sample combs, cooling pack, dummy plate, casting base and casting upstand (see page 45) omniPAGE Mini, 10 x 10cm includes dummy plate, cooling pack 10 x 10cm Casting Base VS10PGS1 10 x 10cm Plain Glass Plates with 1mm Bonded Spacers (pk/2) Replacement Silicone Mat for 10 x 10cm Casting Base VS10NGS1.5 10 x 10cm Notched Glass Plates with 1.5mm Bonded Spacers (pk/2) External Casting Upstand - No Casting Base VS10PGS1.5 10 x 10cm Plain Glass Plates with 1.5mm Bonded Spacers (pk/2) Inner Running Module, with Sliding Clamps and Screw Clamps VS10NGS2 10 x 10cm Notched Glass Plates with 2mm Bonded Spacers (pk/2) Mini Cooling Pack VS10PGS2 10 x 10cm Plain Glass Plates with 2mm Bonded Spacers (pk/2) 10 x 10cm Notched Glass Plates 2mm thick (pk/2) VS10DP Dummy Plate, 10 x 10cm 10 x 10cm Plain Glass Plates 2mm thick (pk/2) VS10S0.75 10cm Spacers - 0.75mm (pk/2) 10 x 10cm Notched Glass Plates with 0.75mm Bonded Spacers (pk/2) VS10S1 10cm Spacers - 1mm thick (pk/2) 10 x 10cm Plain Glass Plates with 0.75mm Bonded Spacers (pk/2) VS10S1.5 10cm Spacers - 1.5mm thick (pk/2) 10 x 10cm Notched Glass Plates with 1mm Bonded Spacers (pk/2) VS10S2 10cm Spacers - 2mm thick (pk/2)

simple, rapid, leak-proof GEL CASTING....

11

• Dual purpose PAGE module eliminates time-consuming transfer of glass plates between separate casting and running • Ground glass plates with bonded injection moulded spacers consistent with comb thickness ensure ‘clean’ well formation, as well as the correct alignment for leak-free casting; also eliminate the need for easily mislaid and awkward to use spacer aligners • Very forgiving, ultra-soft silicone mat within cam-caster compensates for glass plate misalignment to ensure leak-free casting

10 10 44

a

c

b

d 5 5

66 22 Insert glass plates between pressure frame and gasket

Slide gates to make efficient seal

Transfer to casting base and tighten cams

Introduce gel solution and comb and allow to polymerise

33

e 77

8 8

99

Transfer to tank and fill with buffer

f Mini component parts

CODE

DESCRIPTION

VS10-1-0.75 VS10-5-0.75 VS10-8MC-0.75 VS10-9-0.75 VS10-10-0.75 VS10-12-0.75 VS10-16MC-0.75 VS10-20-0.75

Comb 1 Prep, 1 Marker, 0.75mm thick Comb 5 sample, 0.75mm thick Comb 8 sample MC, 0.75mm thick Comb 9 sample, 0.75mm thick Comb 10 sample, 0.75mm thick Comb 12 sample, 0.75mm thick Comb 16 sample MC, 0.75mm thick Comb 20 sample, 0.75mm thick

VS10-1-1 VS10-5-1 VS10-8MC-1 VS10-9-1 VS10-10-1 VS10-12-1 VS10-16MC-1 VS10-20-1

Comb 1 Prep, 1 Marker, 1mm thick Comb 5 sample, 1mm thick Comb 8 sample MC, 1mm thick Comb 9 sample, 1mm thick Comb 10 sample, 1mm thick Comb 12 sample, 1mm thick Comb 16 sample MC, 1mm thick Comb 20 sample, 1mm thick

COMBS, SPACERS AND ACCESSORIES ALSO AVAILABLE IN 0.5MM

Flat, level gasket prevents current leakage from inner buffer chamber

SAMPLE VOLUME

1. Lid and power cables 2. PAGE module 3. Sliding clamps 4. Glass plates 5. Inner buffer chamber

Sliding gates allow optimum pressure to be applied and so create leak-free seal

Colour

Colour

Load samples using Loading Guides and run

Cam pins lock PAGE module onto the ultra-soft silicone mat within the casting base to provide leak-free seal

6.

7.

8.

9.

Gasket Outer tank Cam-pin caster Ultra-soft casting mat 10. Combs

SAMPLE VOLUME

CODE

DESCRIPTION

500µl 70µl 40µl 35µl 30µl 25µl 20µl 15µl

VS10-1-1.5 VS10-5-1.5 VS10-8MC-1.5 VS10-9-1.5 VS10-10-1.5 VS10-12-1.5 VS10-16MC-1.5 VS10-20-1.5

Comb 1 Prep, 1 Marker, 1.5mm thick Comb 5 sample, 1.5mm thick Comb 8 sample MC, 1.5mm thick Comb 9 sample, 1.5mm thick Comb 10 sample, 1.5mm thick Comb 12 sample, 1.5mm thick Comb 16 sample MC, 1.5mm thick Comb 20 sample, 1.5mm thick

1000µl 140µl 80µl 70µl 30µl 50µl 40µl 30µl

650µl 100µl 60µl 50µl 40µl 35µl 25µl 20µl

VS10-1-2 VS10-5-2 VS10-8MC-2 VS10-9-2 VS10-10-2 VS10-12-2 VS10-16MC-2 VS10-20-2

Comb 1 Prep, 1 Marker, 2mm thick Comb 5 sample, 2mm thick Comb 8 sample MC, 2mm thick Comb 9 sample, 2mm thick Comb 10 sample, 2mm thick Comb 12 sample, 2mm thick Comb 16 sample MC, 2mm thick Comb 20 sample, 2mm thick

1300µl 200µl 120µl 100µl 80µl 70µl 50µl 40µl

PER wELL

MC = multichannel pipette compatible

PER wELL

29

• ECONOMy

OF BENCH SPACE

AND COST

• ENHANCED • SIMPLE • RAPID

REPRODUCIBILITy

TO USE CASTING

SET UP COOLING

MINI VERTICAL

modular system 1 2

5

3 The omniPAGE range of Modular Vertical Gel Systems allow multiple electrophoresis techniques to be performed in the same unit. Using the same main tank and lid, three different modules are interchangeable for PAGE, tube gel and electroblotting techniques. These systems are available in three size options, MINI 10 x 10cm, MINI Wide 20 x 10cm and MAXI WAVE 20 x 20cm and MAXI Plus 20 x 30cm, as individual, combination or complete modular systems. Combination and modular systems offer maximum value and space and have the added benefit of increased consistency which results from using the same universal base and lid.

For advance casting, see pages 41, 45

4

7

6 COMPONENTS OF OMNIPAGE MINI MODULAR SYSTEM 1 Tank, Lid and PAGE Module 2 Electroblotting Module 3 Capillary Electrophoresis Module 4 Casting Base 5 6 7 8

Combs Glass Plates with bonded spacers Cooling Pack Blanking Plate

Technical Specifications

8

This system includes all modules and accessories required for Mini Slab Gel Electrophoresis, 2-D Electrophoresis and Electroblotting. The central component of these Systems is the omniPAGE Mini Vertical Unit. This includes a rapid and intuitive casting system, enhanced and easy to set up cooling system and increased gel capacity - each can run up to four gels per run*. OmniPAGE Tube gel modules are capable of resolving up to 10 first dimension gels and the Electroblotting modules have a four blot capacity. This package includes all the necessary accessories for Slab Gel, First Dimension and Electroblotting. Each of these techniques benefits from rapid set up cooling packs which provide enhanced resolution even during high intensity 2-D electrophoresis and electroblotting.

PLATE DIMENSIONS, 10 X 10CM (W X L) GEL DIMENSIONS, 7.5 X 8CM (W X L) UNIT DIMENSIONS

19 X 13 X 15CM (W X D X H)

MAX. SAMPLE CAPACITY

PAGE - 80 SAMPLES, 20 SAMPLES /GEL BLOT - 4 BLOTS 2D - 10 TUBES

BUFFER VOLUME

MIN 250ML;

MAX 1200ML

COMBS AVAILABLE : NO. OF SAMPLES 1, 5, 8MC, 9, 10, 12, 16MC, 20 THICKNESSES 0.75, 1, 1.5, 2MM

High Intensity Blotting MODULE The Hi-Intensity Blot Module comprises Plate electrodes, just 2cm apart, to generate a powerful electric field. This provides transfer capability for up to two 10x10cm gels in as little as one hour.

ORDERING INFORMATION BCVS10CES

Complete Mini (10x10cm) Vertical Electrophoresis Modular System, comprising: 1x omniPAGE Mini Vertical Unit which includes: 2x2mm thick notched glass plates, 2x2mm thick plain glass plates with 1mm thick bonded spacers, 1x dummy plate, 2x combs (1mm thick, 12 samples), 1x casting base, silicone mat, cooling pack plus: 1x Capillary Electrophoresis Module which includes: internal running module for tube gels, capillary tubes, blanking plugs, 2-D combs and spacers plus: 1x Electroblotting Module comprising: internal electroblotting module, 4x compression cassettes for gel sizes up to 10x10cm and 8x fibre pads

BCVS10C2DS

Complete omniPAGE Mini 2-D System, comprising: 1x omniPAGE Mini Vertical Unit which includes: 2x2mm thick notched glass plates, 2x2mm thick plain glass plates with 1mm thick bonded spacers, 1x dummy plate, 2x combs (1mm thick, 12 samples), 1x casting base, silicone mat, cooling pack plus: 1x Capillary Electrophoresis Module which includes: internal running module for tube gels, capillary tubes, blanking plugs, 2-D combs and spacers omniPAGE Mini Tube Gel Unit, 10x10cm with tank and lid, MCT10 Mini Capillary Tubes, 1.0mm, pk/10 glass capillary tubes, blanking ports and cooling pack MCT101.5 Mini Capillary Tubes, 1.5mm, pk/10 omniPAGE Mini Tube Gel Module - includes glass tubes and blanking ports MCBP Mini Capillary Blanking ports * requires additional glass plates and combs

BCVS10DC BCVS10DCI

30

electroblotting omniPAGE Electroblotting Systems include both modules required for Slab Gel Electrophoresis and Electroblotting along with accessories, to provide a complete Mini Vertical gel casting, running and electroblotting system. The

modules included in omniPAGE MINI Vertical Electrophoresis & Blotting System, BCVS10CBS or BCVS10CBS-HI

electroblotting module includes four inter-locking cassettes and eight fibre pads. A high intensity current is generated by coiled platinum electrodes. These, in conjunction with the advanced cooling features, allow rapid electroblotting in just 1-2 hours.

Complete stand-alone units BCSB10 for 10x10cm electroblotting formats, comprise omniPAGE tank and lid, electroblotting module, cassettes and fibre pads. Modules are interchangeable with omniPAGE slab and tube gel modules. Hinged rigid cassettes allow rapid set up and convenience and ensure even contact between the gel and membrane is maintained.

For combs, see page 30

2-D

• HINGED CASSETTES • 4 BLOT CAPACITy • STANDARD AND HIGHINTENSITy OPTIONS • RAPID SET-UP COOLING

Technical Specifications PLATE DIMENSIONS, 10 X 10CM (W X L) GEL DIMENSIONS, 7.5 X 8CM (W X L) omniPAGE Blot Mini Unit, BCSB10

omniPAGE Blot Mini Hi-Intensity Unit, BCSB10-HI

systems

MAX. SAMPLE CAPACITY

PAGE - 80 SAMPLES, 20 SAMPLES /GEL BLOT - 4 BLOTS

BUFFER VOLUME

MIN 250ML;

• • • •

The omniPAGE 2-D Systems include both modules required for Slab Gel and First Dimension Electrophoresis and accessories, to provide a complete MINI 2-D system. The Tube Gel Module

MAX 1200ML

RAPID SET-UP ELECTRO-FOCUSING 10 TUBE CAPACITy EXTENDED ACCESSORy RANGE ENHANCED COOLING FEATURES

includes a rapid release gasket for easy tube extraction. Focusing can be accomplished in as little as three hours. Disposable capillary tubes are included in addition to 2-D combs and spacers which are colour coded according to thickness for easy identification.

A complete stand-alone unit, BCVS10DC, for first dimension capillary/tube gel electrophoresis, comprises omniPAGE tank and lid, tube gel module, tubes and blanking ports.

Units are interchangeable with omniPAGE slab gel and electroblotting modules.

Technical Specifications PLATE DIMENSIONS, 10 X 10CM (W X L) GEL DIMENSIONS, 7.5 X 8CM (W X L) omniPAGE Mini Tube Gel Unit, BCVS10DC

MAX. SAMPLE CAPACITY

PAGE - 80 SAMPLES, 20 SAMPLES /GEL 2D - 10 TUBES

BUFFER VOLUME

MIN 250ML;

MAX 1200ML

ORDERING INFORMATION

Complete omniPAGE Mini (10 x 10cm) Vertical Electrophoresis & Blotting System, comprising: 1x omniPAGE Mini Vertical Unit which includes: 2x2mm thick notched glass plates, 2x2mm thick plain glass plates with 1mm thick bonded spacers, 1x dummy plate, 2x combs (1mm thick, 12 samples), 1x casting base, silicone mat, cooling pack. plus: 1x Electroblotting Module which includes: internal electroblotting module, 4x compression cassettes for gel sizes up to 10x10cm and 16x fibre pads.

BCVS10CBS-HI Complete omniPAGE Mini (10 x 10cm) Vertical Electrophoresis & High Intensity Blotting System, comprising: 1x omniPAGE Mini Vertical Unit which includes: 2x2mm thick notched glass plates, 2x2mm thick plain glass plates with 1mm thick bonded spacers, 1x dummy plate, 2x combs (1mm thick, 12 samples), 1x casting base, silicone mat, cooling pack. plus: 1x High Intensity Electroblotting Module which includes: internal electroblotting module, 2x compression cassettes for gel sizes up to 10x10cm and 8x fibre pads.

BCVS10CBS

BCSB10 VS10BI SB10C

omniPAGE Blot Mini Unit, 10 x 10cm System including tank and lid, 4 cassettes, 16 fibre pads, cooling pack omniPAGE Blot Mini Module - includes 4 cassettes and 16 fibre pads omniPAGE Blot Mini Cassette

BCSB10-HI VS10BI-HI SB10F

omniPAGE Blot Mini Unit,10 x 10cm, High Intensity System including tank and lid, 2 cassettes, 8 fibre pads, cooling pack omniPAGE Blot Mini High Intensity Module- includes 2 cassettes and 8 fibre pads Fibre pads - pk/8

31

OMNIPAGE

mini wide • OFFERS

THE CAPACITy OF TwO

MINI GELS ON A SINGLE GEL

• RAPID

GEL CASTING AND

LOADING

• OPTIONAL

LOw OR HIGH

BUFFER VOLUMES

• RAPID

SET UP COOLING

The omniPAGE Mini Wide vertical gel unit, with a gel width of 20cm, effectively allows double the number of samples to be resolved as the omniPAGE Mini. This allows consistency of sample comparison on a single gel and is designed for those with greater than 20 samples to compare and resolve. Simple set up using ultra soft silicone seals guarantees trouble free glass plate loading and gel casting.

NEW

Dual gaskets on the gel running module along with notched and plain glass plates ensure leak proof gel running.

Rapid set up cooling retains resolution in extended separations and also saves on buffer volume without affecting run quality.

4mm thick glass plates reduce breakage and have bonded spacers for added convenience. A wide range of accessories is available allowing many techniques to be performed using the same unit. Prep combs can be used to maximise sample loading and recovery. Accessory electroblotting and tube gel modules are available which use the same outer tank and lid.

Technical Specifications PLATE DIMENSIONS, GEL DIMENSIONS,

uses same combs as WAVE MAXI systems

20 X 10CM (W X H) 18 X 8CM (W X H)

UNIT DIMENSIONS

26 X 16 X 16CM (W X D X H)

MAX. SAMPLE CAPACITY

PAGE - 192 SAMPLES, 48 SAMPLES /GEL BLOT - 4 BLOTS/GEL 2D - 10 TUBES

BUFFER VOLUME

MIN 600ML; MAX 2800ML

COMBS AVAILABLE : NO. OF SAMPLES THICKNESSES

1, 5, 10, 18MC, 24, 30, 36MC, 48 0.75, 1, 1.5, 2MM

modular system This system includes all modules and accessories required for Mini Wide Slab Gel Electrophoresis, 2-D Electrophoresis and Electroblotting. The central component of these

Typical Applications Mini SDS PAGE, Native PAGE, Gradient, Second dimension and Nucleic acid separations

modules included in omniPAGE MINI WIDE, BCVS10WCES

Systems is the omniPAGE Mini Wide Vertical Unit. This incorporates a rapid and intuitive casting system, enhanced and easy to set up cooling system and increased gel capacity - each can run up to four gels per run*. omniPAGE Tube gel modules are capable of resolving up to 10 first dimension gels and the Electroblotting modules have four blot capacity. Each of these techniques benefits from rapid set up cooling packs which provide enhanced resolution even during high intensity 2-D electrophoresis and electroblotting.

ORDERING INFORMATION BCVS10WD BCVS10WDSYS VS20CAST VS20DCASTM BCVS10WDIRM VS20-x -LG VS10WNG VS10WPG VS10WNGS0.75 VS10WPGSO.75

omniPAGE Mini Wide, 20 x 10cm Dual, 2 sets of Glass Plates with 1mm thick bonded Spacers, 2 x 24 sample, 1mm thick combs, cooling pack omniPAGE Mini Wide, 20 x 10cm Dual, 2 sets of Glass Plates with 1mm thick bonded Spacers, 2 x 24 sample, 1mm thick combs, cooling pack including caster 20 x 10cm Casting Base VS10WNGS1 20 x 10cm Notched Glass Plates with 1mm Bonded Spacers (pk/2) VS10WPGS1 20 x 10cm Plain Glass Plates with 1mm Bonded Spacers (pk/2) Replacement Silicone Mat for 20 x 10cm Casting Base Inner Running Module VS10WPGS1.5 20 x 10cm Plain Glass Plates with 1.5mm Bonded Spacers (pk/2) Loading guides for omniPAGE mini combs, x = comb well number VS10WPGS2 20 x 10cm Plain Glass Plates with 2mm Bonded Spacers (pk/2) 20 x 10cm Notched Glass Plates 4mm thick (pk/2) VS10WDP Dummy Plate, 20 x 10cm 20 x 10cm Plain Glass Plates 4mm thick (pk/2) RPW-0.2100 Replacement Platinum Wire - 0.2mm, 50cm VS20ICB Cooling Pack 20 x 10cm Notched Glass Plates with 0.75mm Bonded Spacers (pk/2) 20 x 10cm Plain Glass Plates with 0.75mm Bonded Spacers (pk/2)

BCVS10WCES

Complete Mini Wide (20x10cm) Vertical Electrophoresis Modular System, comprising: 1x omniPAGE Mini Wide Vertical Unit which includes: 2x4mm thick notched glass plates, 2x4mm thick plain glass plates with 1mm thick bonded spacers, 1x dummy plate, 2x combs (1mm thick, 24 samples), 1x casting base, silicone mat, cooling pack. plus: 1x Capillary Electrophoresis Module which includes: internal running module for tube gels, capillary tubes, blanking plugs, 2-D combs and spacers plus: 1x Electroblotting Module comprising: internal electroblotting module, 4x compression cassettes for gel sizes up to 20x10cm and 8x fibre pads.

32

* requires additional glass plates and combs

electroblotting omniPAGE Electroblotting Systems include both modules required for Slab Gel Electrophoresis and Electroblotting along with accessories, to provide a complete Mini Wide Vertical gel casting, running and electroblotting system.

• HINGED • 4

20x10cm electroblotting formats, comprise omniPAGE tank and lid, electroblotting module, cassettes and fibre pads. Modules are interchangeable with omniPAGE Mini Wide slab and tube gel modules. Hinged rigid cassettes allow rapid set up and convenience and ensure even contact between the gel and membrane is maintained.

2-D

• RAPID

modules included in omniPAGE MINI WIDE Blot System, BCVS10WCBS

For combs, see page 35

HIGH

INTENSITy

SET-UP COOLING

Technical Specifications PLATE DIMENSIONS, 20 X 10CM (W X L) GEL DIMENSIONS, 18 X 8CM (W X L) MAX. SAMPLE CAPACITY

PAGE - 192 SAMPLES, 48 SAMPLES /GEL BLOT - 4 BLOTS/GEL

BUFFER VOLUME

MIN 600ML;

COMBS AVAILABLE : NO. OF SAMPLES THICKNESSES

The omniPAGE 2-D Systems include both modules required for Slab Gel and First Dimension Electrophoresis and accessories, to provide a complete Mini Wide 2-D system.

• RAPID

MAX 2800ML

1, 5, 10, 18MC, 24, 30, 36MC, 48 0.75, 1, 1.5, 2MM

SET-UP

ELECTROFOCUSING

The Tube Gel Module includes a rapid release gasket for easy tube extraction.

Focusing can be accomplished in as little as three hours. Disposable capillary tubes are included in addition to 2-D combs and spacers which are colour coded according to thickness for easy identification.

gel electrophoresis, comprises omniPAGE tank and lid, tube gel module, tubes and blanking ports. Units are interchangeable with omniPAGE Mini Wide slab gel and electroblotting modules.

AND

OPTIONS

systems

A complete stand-alone unit, BCVS10WDC, for first dimension capillary/tube

BLOT CAPACITy

• STANDARD

The electroblotting module includes four inter-locking cassettes and eight fibre pads. A high intensity current is generated by coiled platinum electrodes. These in conjunction with the advanced cooling features allow rapid electroblotting in just 1-2 hours.

Complete stand-alone units BCSBW10 for

CASSETTES

• 10

TUBE CAPACITy

• EXTENDED • ENHANCED

ACCESSORy RANGE COOLING FEATURES

Technical Specifications modules included in omniPAGE MINI WIDE 2D System, BCVS10WC2DS

PLATE DIMENSIONS, 20 X 10CM (W X L) GEL DIMENSIONS, 18 X 8CM (W X L) MAX. SAMPLE CAPACITY

PAGE - 192 SAMPLES, 48 SAMPLES /GEL 2D - 10 TUBES

BUFFER VOLUME

MIN 250ML;

COMBS AVAILABLE : NO. OF SAMPLES THICKNESSES

MAX 1200ML

1, 5, 10, 18MC, 24, 30, 36MC, 48 0.75, 1, 1.5, 2MM

ORDERING INFORMATION BCVS10WCBS

BCSB10W VS10WBI

Complete omniPAGE Mini Wide (20 x 10cm) Vertical Electrophoresis & Blotting System, comprising: 1x omniPAGE Mini Wide Vertical Unit which includes: 2x4mm thick notched glass plates, 2x4mm thick plain glass plates with 1mm thick bonded spacers, 1x dummy plate, 2x combs (1mm thick, 24 samples), 1x casting base, silicone mat, cooling pack. plus: 1x Wide Electroblotting Module which includes: internal electroblotting module, 4x compression cassettes for gel sizes up to 20x10cm and 8x fibre pads. omniPAGE Blot Mini Wide Cassette SB10WC omniPAGE Blot Mini Wide Unit, 20 x 10cm System including tank and lid, Fibre pads - pk/8 SB10WF 4 cassettes, 8 fibre pads, cooling pack omniPAGE Blot Mini Wide Module - includes 4 cassettes and 8 fibre pads.

BCVS10WC2DS Complete omniPAGE Mini Wide 2-D System, comprising: 1x omniPAGE Mini Wide Vertical Unit which includes: 2x4mm thick notched glass plates, 2x4mm thick plain glass plates with 1mm thick bonded spacers, 1x dummy plate, 2x combs (1mm thick, 24 samples), 1x casting base, silicone mat, cooling pack. plus: 1x Wide Capillary Electrophoresis Module which includes: internal running module for tube gels, capillary tubes, blanking plugs, 2-D combs and spacers.

BCVS10WDC omniPAGE Mini Wide Tube Gel Unit, 20x10cm with tank and lid, MCT10 Mini Capillary Tubes, 1.0mm, pk/10 glass capillary tubes, blanking ports and cooling pack MCT101.5 Mini Capillary Tubes, 1.5mm, pk/10 BCVS10WDCI omniPAGE Mini Wide Tube Gel Module - includes glass tubes and blanking ports MCBP Mini Capillary Blanking ports

33

• RUN

UP TO

FOUR GELS [TETRAD

SIMULTANEOUSLy

SySTEMS]

• ONLy FOUR SCREwS REqUIRED TO SECURE GLASS PLATES - REDUCES SET UP TIME SIGNIFICANTLy

• VERTICAL

SCREw-CLAMPS

DISTRIBUTE PRESSURE EVENLy ALONG THE HEIGHT OF THE GEL TO PREVENT PLATE BOwING AND GEL COMPRESSION

MAXI VERTICAL

omnipage The new Maxi ‘WAVE’ System is designed to perform a variety of separations, including first- and seconddimension SDS-PAGE, native, preparative, gradient and highresolution nucleic acid electrophoresis, plus capillary tube gel IEF and electroblotting. The omniPAGE WAVE is one of the most versatile maxi vertical systems available.

NEW

• DETACHABLE INNER COOLING COIL FACILITATES RAPID AND UNIFORM, SMILE-FREE ELECTROPHORESIS, EVEN AT HIGHER VOLTAGES

• INjECTION MOULDED CONSTRUCTION GUARANTEES LONG LIFE wITH RELIABLE AND CONSISTENT PERFORMANCE

Technical Specifications NUMBER OF GELS PLATE DIMENSIONS (W X H X T) STANDARD SPACER DIMENSIONS (W X H) IPG SPACER DIMENSIONS (W X H) TOTAL VOLUME INNER BUFFER CHAMBER TOTAL BUFFER VOLUME FOR 2 GELS TOTAL BUFFER VOLUME FOR 4 GELS STANDARD RUN TIME FOR SDS-PAGE WITHOUT COOLING WITH COOLING

MAX. SAMPLE CAPACITY COMBS AVAILABLE : NO. OF SAMPLES THICKNESSES UNIT DIMENSIONS (W X D X H) WEIGHT

1-4 20 X 20 X 0.4CM 2 X 20CM 0.6 X 20CM 640ML 5.3L 4.8L 4 - 5 HOURS 3 - 4 HOURS 192 SAMPLES, 48/GEL 1, 5, 10, 18MC, 24, 30, 36MC, 48 0.75, 1, 1.5, 2MM 30 X 18 X 27CM 2.5KG

ORDERING INFORMATION BCWAVED BCWAVESYS BCWAVESYS-CU BCWAVETETRAD1 VS20WAVE-EC VS20WAVECAST VS20WAVEDIRM VS20WAVE-CC VS20DCAST VS20DCASTM VS20DIRM VS20ICB VS20-x -LG VS20NG VS20PG VS20NGS0.75 VS20PGS0.75

34

The new, innovative, vertical screw-clamp system within the PAGE insert requires only four screws to secure up to four 20x20cm gels. This gives the omniPAGE WAVE the selective advantage of a much faster set up time compared to products whose traditional clamping configurations require as many as 24 screws to secure just two glass plates. In addition, the WAVE’s innovative vertical screw-clamp configuration distributes pressure evenly along the height of the gel rather than in the centre to eliminate plate bowing and gel

External Casting Upstand is basically a standard internal module but without Platinum wire

compression. This still maintains a leak-proof seal during casting; while the ergonomic wave-like design of he PAGE insert aids both handling and set up.

A detachable inner cooling coil connects to the laboratory water supply or a recirculating chiller to provide uniform, smile-free electrophoresis, while allowing runs to be performed at higher voltage. Whether it be running 2 or 4 gels, electroblotting, and IEF using capillary tube gels or IPG strips, all of these techniques may be performed using the same multi-purpose tank. And of course, this is in addition to retaining the benefits of large format electrophoresis, such as extended separation distances, greater sample throughput and superior resolution.

WAVE MAXI TETRAD A 4-gel omniPAGE WAVE TETRAD system is created simply by introducing additional plates with spacers and appropriate combs. WTETRAD is supplied with a casting base and external casting upstand to allow gels to be prepared in advance, ready for the next run.

omniPAGE WAVE Maxi, 20 x 20cm Dual with Glass Plates with bonded 1mm thick spacers, 2x 24 sample combs, cooling pack and dummy plate omniPAGE WAVE Maxi, 20 x 20cm Dual with Glass Plates with bonded 1mm thick spacers, 2x 24 sample combs, cooling pack, dummy plate and casting base omniPAGE WAVE Maxi, 20 x 20cm Dual with Glass Plates with bonded 1mm thick spacers, 2x 24 sample combs, cooling pack, dummy plate, casting base and external casting upstand omniPAGE WAVE Maxi, 20 x 20cm Dual with Glass Plates with bonded 1mm thick spacers, 2x 24 sample combs, cooling pack, dummy plate, casting base and external casting upstand, PLUS 2x pks/2 notched glass plates with 1mm bonded spacers and 2x 1mm 24-sample combs VS20 WAVE External Casting Stand - No Casting Base VS20NGS1 20 x 20cm Notched Glass Plates with 1mm Bonded Spacers (pk/2) VS20 WAVE Casting Base VS20PGS1 20 x 20cm Plain Glass Plates with 1mm Bonded Spacers (pk/2) VS20 WAVE PAGE module VS20PGS1.5 20 x 20cm Plain Glass Plates with 1.5mm Bonded Spacers (pk/2) Detachable Cooling Coil VS20PGS2 20 x 20cm Plain Glass Plates with 2mm Bonded Spacers (pk/2) omniPAGE WAVE Maxi, 20 x 20cm Dual Caster VS20DP Dummy Plate, 20 x 20cm Replacement Rubber mats for 20 x 20cm Caster VS20S0.75 20cm Spacers - 0.75mm (pk/2) omniPAGE WAVE Maxi - Inner Running Module VS20S1 20cm Spacers - 1mm thick (pk/2) WAVE Maxi Cooling Pack VS20S1.5 20cm Spacers - 1.5mm thick (pk/2) Loading guides for omniPAGE WAVE Maxi combs, x = comb well number VS20S2 20cm Spacers - 2mm thick (pk/2) 20 x 20cm Notched Glass Plates 4mm thick (pk/2) RPW-0.2100 Replacement Platinum Wire - 0.2mm, 100cm 20 x 20cm Plain Glass Plates 4mm thick (pk/2) VS20WAVE-IEFKIT IEF Conversion for 18cm IPG strips and tube gels, includes: 1 set of plain glass plates 20 x 20cm Notched Glass Plates with 0.75mm Bonded Spacers (pk/2) with bonded spacers, 0.6x20cm (WxH); and 2x 2-D combs with one 3.5mm marker 20 x 20cm Plain Glass Plates with 0.75mm Bonded Spacers (pk/2) lane and one 18cm preparatory well

simple, rapid, leak-proof GEL CASTING....

• Dual purpose PAGE module eliminates time-consuming transfer of glass plates between separate casting and running

1

• Ground glass plates with bonded injection moulded spacers consistent with comb thickness ensure ‘clean’ well formation, as well as the correct alignment for leak-free casting; also eliminate the need for easily mislaid and awkward to use spacer aligners • Very forgiving, ultra-soft silicone mat within cam-caster compensates for glass plate misalignment to ensure leak-free casting

a

c

b

12

d

5 3 Assemble each gel cassette on a flat level surface, by placing the plain glass plate down with the spacers facing upwards followed by the notched glass plate

e

Loosen the vertical screw pins in the PAGE module to release the locking mechanism, allowing the gel clamps to sit in the resting slots

Insert a gel cassette into each side of the inner buffer chamber in the PAGE module, and begin tightening the vertical screw-pins

Continue to tighten the screw pins until the gel clamps glide out of the resting slots and fix firmly against the glass plates pushing them upright

Vertical screw pins, colour-coded to prevent polarity reversal, push the gel clamps out of the resting slots to secure glass plates firmly within the PAGE module

6

2

4

7

8

9

f

Resting slots allow the gel clamps to sit conveniently out of the way, to aid hindrance-free loading of the cassettes into the PAGE module

11

Cam pins lock PAGE insert onto the ultra-soft silicone mat within the casting base to provide leak-free seal

Insert cams and turn until tight, drawing the PAGE module onto the casting to form a leak-proof seal

Colour

10

Ergonomic ‘wave’ design of PAGE module provides convenient finger grips for easy handling

g

h

Pour in the gel solution, insert the combs and allow the wells to polymerise

Transfer the PAGE module to gel the tank. Fill the inner and outer buffer chambers before loading samples

Code

Description

VS20-1-0.75 VS20-5-0.75 VS20-10-0.75 VS20-18MC-0.75 VS20-24-0.75 VS20-30-0.75 VS20-36MC-0.75 VS20-48-0.75

Comb 1 Prep, 1 Marker, 0.75mm thick Comb 5 sample, 0.75mm thick Comb 10 sample, 0.75mm thick Comb 18 sample MC, 0.75mm thick Comb 24 sample, 0.75mm thick Comb 30 sample, 0.75mm thick Comb 36 sample MC, 0.75mm thick Comb 48 sample, 0.75mm thick

VS20-1-1 VS20-5-1 VS20-10-1 VS20-18MC-1 VS20-24-1 VS20-30-1 VS20-36MC-1 VS20-48-1

Comb 1 Prep, 1 Marker, 1mm thick Comb 5 sample, 1mm thick Comb 10 sample, 1mm thick Comb 18 sample MC, 1mm thick Comb 24 sample, 1mm thick Comb 30 sample, 1mm thick Comb 36 sample MC, 1mm thick Comb 48 sample, 1mm thick

Flat, level gasket prevents current leakage from inner buffer chamber

Sliding gel clamps available in two thicknesses to accommodate single- and double-gel cassettes

Replace the lid, connect to the power supply and run

Sample Volume per well

WAVE Maxi component parts 1. Lid and power cables 2. PAGE module 3. Vertical screw-pin 4. Clamping bars 5. Glass plates 6. Inner buffer chamber

i

Colour

Check the bottom of the glass plates to ensure that they are flush together, and place the PAGE module on the casting base; make sure that the cams are facing downwards

7. Gasket 8. Detachable cooling coil 9. Outer tank 10. Cam-pin caster 11. Ultra-soft casting mat 12. Combs

Sample Volume per well

Code

Description

1100µl 160µl 80µl 40µl 30µl 25µl 20µl 15µl

VS20-1-1.5 VS20-5-1.5 VS20-10-1.5 VS20-18MC-1.5 VS20-24-1.5 VS20-30-1.5 VS20-36MC-1.5 VS20-48-1.5

Comb 1 Prep, 1 Marker, 1.5mm thick Comb 5 sample, 1.5mm thick Comb 10 sample, 1.5mm thick Comb 18 sample MC, 1.5mm thick Comb 24 sample, 1.5mm thick Comb 30 sample, 1.5mm thick Comb 36 sample MC, 1.5mm thick Comb 48 sample, 1.5mm thick

2200µl 320µl 160µl 80µl 60µl 50µl 40µl 30µl

1500µl 200µl 100µl 50µl 40µl 35µl 25µl 20µl

VS20-1-2 VS20-5-2 VS20-10-2 VS20-18MC-2 VS20-24-2 VS20-30-2 VS20-36MC-2 VS20-48-2

Comb 1 Prep, 1 Marker, 2mm thick Comb 5 sample, 2mm thick Comb 10 sample, 2mm thick Comb 18 sample MC, 2mm thick Comb 24 sample, 2mm thick Comb 30 sample, 2mm thick Comb 36 sample MC, 2mm thick Comb 48 sample, 2mm thick

3000µl 400µl 200µl 100µl 80µl 70µl 50µl 40µl

35

modular 1 4

5

3 • ECONOMy

OF BENCH SPACE

AND COST

• ENHANCED • SIMPLE

REPRODUCIBILITy

2

TO USE CASTING

• SCALE-UP

DISCOVERy

PROjECTS USING A LARGE

FORMAT, HIGH-RESOLUTION SySTEM

6

tank and lid, three different modules are interchangeable for PAGE, tube gel and electroblotting techniques. These systems are available in three size options, MINI 10 x 10cm, MINI Wide 20 x 10cm and MAXI WAVE 20 x 20cm, as individual, combination or complete modular systems. Combination and modular systems offer maximum value and space and have the added benefit of increased consistency which results from using the same universal base and lid.

MAXI VERTICAL

The omniPAGE range of Modular Vertical Gel Systems allow multiple electrophoresis techniques to be performed in the same unit. Using the same main

The omniPAGE WAVE complete electrophoresis system provides a fully integrated solution using one universal gel tank and lid for vertical PAGE, tube gel IEF and electroblotting.

This concept of interchangeable module allows users to combine vertical PAGE with capillary gel IEF to perform 2-D electrophoresis, followed by western transfer for enhanced sensitivity. The supplied detachable internal Cooling Coil allows connection to either the laboratory water supply or a recirculating chiller, and so makes the systems ideal for:

For advance casting, see page 41

COMPONENTS OF WAVE MAXI MODULAR SYSTEM 1 Tank and Lid 2 PAGE Module 3 Electroblotting Module 4 Hi-Intensity Electroblotting Module 5 Capillary Electrophoresis Module 6 Casting Base

• temperature-sensitive separations and samples and/or • application of high voltages to achieve precise, high-resolution focusing for pI determination

Blotting

Technical Specifications UNIT DIMENSIONS,

19 X 13 X 15CM (W X D X H)

MAX. SAMPLE CAPACITY

PAGE - 80 SAMPLES, 20 SAMPLES /GEL BLOT - 4 BLOTS 2D - 10 TUBES

BUFFER VOLUME

MIN 4800ML;

MAX 5300ML

The Standard Platinum Wire module can accommodate up to four 20x20cm, eight 20x10cm or sixteen 10x10cm gels while the optional High-Intensity Plate module facilitates rapid 2-gel transfers.

2D Capillary Electrophoresis Focus up to 10 first-dimension IEF tube gels within a single run using the dedicated 2-D insert, or use the included blanking ports to run fewer gels if preferred.

IEF Conversion for 18cm IPG strips and tube gels, includes: 1 set of plain glass plates with bonded spacers, 0.6x20cm (WxH); and 2x 2-D combs with one 3.5mm marker lane and one 18cm preparatory well

ORDERING INFORMATION BCWAVECES

Complete WAVE Maxi System for PAGE, 2-D electrophoresis and blotting, comprising: 1x WAVE Maxi Vertical Unit which includes: PAGE Module, 2x4mm thick notched glass plates, 2x4mm thick plain glass plates with 1mm thick bonded spacers, 1x dummy plate, 2x combs (1mm thick 24 samples), 1x casting base, silicone mat, cooling coil; plus: 1x WAVE Standard Electroblotting Unit which includes: WAVE Maxi Platinum Wire Blotting Module, 4x compression cassettes and 16x fibre pads; and: 1x WAVEDCI Capillary Electrophoresis Unit which includes: WAVE Maxi Tube Gel Module, 100x capillary tubes,10x blanking ports; and 1x WAVE IEF-KIT: 1 set of plain glass plates with bonded spacers, 0.6x20cm (WxH); and 2x 2-D combs with one 3.5mm marker lane and one 18cm preparatory well.

BCWAVEC2DS

Complete omniPAGE WAVE Maxi 2-D System comprising: 1x WAVE Maxi Vertical Unit which includes: PAGE Module, 2x4mm thick notched glass plates, 2x4mm thick plain glass plates with 1mm thick bonded spacers, 1x dummy plate, 2x combs (1mm thick 24 samples), 1x casting base, silicone mat, cooling pack; plus: 1x WAVEDCI Capillary Electrophoresis Unit which includes: WAVE Maxi Tube Gel Module, 100x capillary tubes,10x blanking ports; and 1x WAVE IEF-KIT: of plain glass plates with bonded spacers, 0.6x20cm (WxH); and 2x 2-D combs with one 3.5mm marker lane and one 18cm preparatory well WAVE Maxi Tube Gel Unit, 20x20cm with tank and lid, MCT20 Maxi Capillary Tubes, 1.0mm, pk/10 glass capillary tubes, blanking ports and cooling pack MCT201.5 Maxi Capillary Tubes, 1.5mm, pk/10 WAVE Maxi Tube Gel Module - includes glass tubes and blanking ports MCBP Maxi Capillary Blanking ports

1 set BCVS20DC BCVS20DCI

36

electroblotting The omniPAGE WAVE Blotting Systems include all necessary components for blotting of vertical slab gels.

Interchangeable modular modules combine vertical PAGE with electro-transfer using the same universal tank and lid.

The electroblotting module includes four inter-locking cassettes and eight fibre pads, with capacity for up to 4 WAVE Maxi gels, 8 omniPAGE Mini Wide or 16 omniPAGE Mini gels. The Standard module with platinum wire electrodes 8cm apart is ideal for optimum capacity - at voltages as low as 35V, while the Hi-Intensity module with coiled platinum plate electrodes, just 4cm apart are perfect for rapid transfers in 1-2 hours, at 200V. A detachable cooling coil, which connects to the laboratory water supply or a recirculating chiller, prevents buffer depletion and so allows overnight transfers while also maintaining the low temperatures important for protein stability during native transfers and also for fast high-intensity blots.

Complete stand-alone units for electroblotting, BCSW20 comprise omniPAGE tank and lid, electroblotting module, cassettes and fibre pads. Modules are interchangeable with omniPAGE slab and tube gel modules. Hinged Open design, rigid cassettes maximise current rigid cassettes allow rapid set up and transfer and eliminate convenience and ensure even contact between ‘shadow band’ formation the gel and membrane is maintained.

2-D

• VERTICAL PAGE AND BLOTTING USING A UNIVERSAL TANk AND LID

• OPEN DESIGN, RIGID CASSETTES

MAXIMISE CURRENT TRANSFER AND ELIMINATE ‘SHADOw BAND’

FORMATION

• COLOUR-CODED

CASSETTES

PREVENT POLARITy REVERSAL

• COOLING

COIL INCLUDED TO

MAINTAIN LOw RUNNING TEMPERATURES

Technical Specifications PLATE DIMENSIONS, 20 X 20 X 0.4CM (W X L X D) GEL DIMENSIONS 16 X 17.5CM (W X L)

omniPAGE WAVE Standard Blotter, BCSW20

MAX. SAMPLE CAPACITY

PAGE - 192 SAMPLES, 48 SAMPLES /GEL BLOT - 4X WAVE GELS 1X HI-INTENSITY WAVE GEL

BUFFER VOLUME

MIN 1200ML; MAX 5300ML

omniPAGE WAVE Hi-Intensity Blotter, BCSW20-HI

For combs, see page 35

systems

• ALL NECESSARy COMPONENTS FOR 2-D ELECTROPHORESIS USING

The omniPAGE WAVE 2-D Systems include all necessary components for 2-D electrophoresis using capillary ampholyte tube gels. Interchangeable modular inserts run capillary tube gels for first-dimension IEF and vertical slab gels for second-dimension with SDSPAGE using the same universal tank and lid. It is possible to focus up to 10 first-dimension IEF tube gels within a single run using the dedicated 2-D insert, or use included blanking ports to run fewer gels if preferred. The system may also be used for 18cm IPG strips resolved in the first-dimension on the omniPAGE flat-bed IEF system.

CAPILLARy AMPHOLyTE TUBE GEL

• INTERCHANGEABLE INSERTS RUN CAPILLARy TUBE GELS FOR FIRSTDIMENSION IEF AND VERTICAL SLAB GELS FOR SECOND-DIMENSION SDS-PAGE USING THE SAME UNIVERSAL TANk AND LID

Technical Specifications PLATE DIMENSIONS, 20 X 20 X 0.4CM (W X L X D) GEL DIMENSIONS, 16 X 17.5CM (W X L)

Complete stand-alone units for first dimension capillary/tube gel electrophoresis are available and comprise omniPAGE WAVE Maxi tank and lid, tube gel module, tubes and blanking ports. Units are interchangeable with omniPAGE WAVE slab gel and electroblotting modules.

MAX. SAMPLE CAPACITY

PAGE - 192 SAMPLES, 48 SAMPLES /GEL 2D - 10 TUBES

BUFFER VOLUME

MIN 1200ML; MAX 5300ML

ORDERING INFORMATION

Complete WAVE Maxi System for electrophoresis and blotting, comprising: 1x WAVE Maxi Vertical Unit which includes: PAGE insert, 2x4mm thick notched glass plates, 2x4mm thick plain glass plates with 1mm thick bonded spacers, 1x dummy plate, 2x combs (1mm thick 24 samples), 1x casting base, silicone mat, cooling pack; plus: 1x WAVE Standard Electroblotting Unit which includes: WAVE Maxi platinum wire Blotting Module, 4x compression cassettes and 16x fibre pads BCWAVECBS-HI Complete WAVE Maxi System for electrophoresis and High Intensity blotting, comprising: 1x WAVE Maxi Vertical Unit which includes: 2x4mm thick notched glass plates, 2x4mm thick plain glass plates with 1mm thick bonded spacers, 1x blanking plate, 2x combs (1mm thick, 24 samples), 1x casting base, silicone mat, cooling pack; plus: 1x High Intensity Electroblotting Unit which includes: internal electroblotting module, 2x compression cassettes for gel sizes up to 20x20cm and 8x fibre pads BCWAVECBS

BCSW20 VS20BI

WAVE Maxi Blot Platinum Wire Unit, 20 x 20cm System including tank and lid, 4 cassettes, 16 fibre pads, cooling pack WAVE Maxi Platinum Wire Blot Module - 4 cassettes and 16 fibre pads

SW20BI-HI SW20C SW20F

WAVE Maxi Hi-Intensity Blot Module - 1x cassette and 8 fibre pads WAVE Maxi Blot Cassette Fibre pads - pk/8

37

VeRtIcAL pAcKAges

4-gel tetrad systems

TETRAD10

TETRAD10-CBS

TETRAD10-PS

TETRAD10-CBS-PS

standard vertical electrophoresis

OMNI10-PS

OMNI10-CBS-PS

OMNI20-CBS-PS

ORDERING INFORMATION TETRAD10

OmniPAGE TETRAD Mini-Protein Electrophoresis Package for 1mm self-cast gels. Includes: - BCVS10DSYS-CU – 10x10cm mini-vertical unit with casting base and external casting stand, plus 2x 1mm 12-sample combs, 2x plain glass plates with 1mm spacer and 2x notched plates (Page 32), VS10-12-1 – 2x additional 1mm 12-sample combs, VS10PGS1 – 1 pack of 2x plain glass plates with 1mm spacer, VS10NG - 1 pack of 2x notched glass plates with 1mm spacer, VS10NGS1 - 2 packs of 2x notched glass plates with 1mm spacer TETRAD10-CBS OmniPAGE TETRAD Mini-Protein Electrophoresis and Blotting Package for 1mm self-cast gels and interchangeable 4-blot module, Includes: - TETRAD10 and VS10BI – 4-blot insert with 4 blotting cassettes and 8 fibre pads (Page 35) TETRAD10-PS OmniPAGE TETRAD with standard Midi power supply option*. Includes: - TETRAD10 - OmniPAGE TETRAD Mini-Protein Electrophoresis Package for 1mm selfcast gels and MP-300 - 300V, 700mA, 150W midi power supply (Page 53) TETRAD10-CBS-PS OmniPAGE TETRAD-CBS with standard Midi power supply*. Includes: - TETRAD10-CBS - OmniPAGE TETRAD10 Mini-Protein Electrophoresis Package for 1mm selfcast gels with blotting module and MP-300 - 300V, 700mA, 150W midi power supply (Page 53) OMNI10-PS OmniPAGE Mini Vertical with standard Midi power supply option*. Includes: - BCVS10DSYS - OmniPAGE Mini Vertical with casting base and MP-300 - 300V, 700mA, 150W midi power supply (Page 53) OMNI10-CBS-PS OmniPAGE Mini Vertical with interchangeable 4-blot module and standard Midi power supply option*. Includes: - OMNI10-PS - OmniPAGE Mini Vertical with casting base, VS10BI – 4-blot insert with 4 blotting cassettes and 8 fibre pads (Page 35) and MP-300V - 300V, 700mA, 150W midi power supply (Page 53) OMNI20-CBS-PS OmniPAGE WAVE Maxi Vertical* with interchangeable 3-blot module and standard Midi power supply option*. Includes: - BCWAVEDYS OmniPAGE WAVE Maxi Vertical with casting base, VS20BI – 3-blot insert with 3 blotting cassettes and 6 fibre pads (Page 35) and MP-300 - 300V, 700mA, 150W midi power supply (Page 53)

38

* 1) all packages available with High Voltage MP-500P Power Supply, 2) WAVE Maxi Tetrad systems also available, please contact for information and pricing

LARGE FORMAT VERTICAL

large format Ideal for a variety of large format vertical gel applications, these units offer advanced features for enhancing gel resolution and ease of use, essential when handling gels of this size. Each unit contains ultra soft silicone seals

• RUN

96

UP TO

• ENHANCED easily replaced buffer seals

for easy plate sealing and trouble free runs, even over extended run times.

SAMPLES

GEL HEAT

HOMOGENISATION

• VARIABLE LOw OR HIGH BUFFER VOLUMES

Resolution is enhanced by using an aluminium heat sink plate, essential for even sample migration. Added convenience is provided by a removable lower buffer tank and upper buffer drainage tap.

• 20

X

50CM

FORMATS

OR

33

X

45CM

Technical Specifications

Special buffer chambers allow either low buffer volumes to be used for economy or high buffer volumes to be used for extended runs.

A wide range of interchangeable comb and spacer options allows a large number of techniques to be easily accomplished including; DNA Sequencing, 2-D analysis, Micro-satellite analysis, DNA fingerprinting, Gel shift assays, Single-Strand Conformation Polymorphism (SSCP), Heteroduplex and Oligonucleotide analysis.

PLATE DIMENSIONS CSQ20 CSQ33 (W X L)

20 X 50CM 33 X 45CM

MAX SAMPLE

48 SAMPLES 96 SAMPLES

CAPACITY

BUFFER VOLUME

CSQ20 CSQ33

CSQ20 MIN 500ML, MAX 1000ML CSQ33 MIN 800ML, MAX 2000ML

COMBS AVAILABLE NO. OF TEETH 24, 48, 80, 96 THICKNESSES 0.25, 0.35, 1, 1.5MM

Typical Applications removable lower buffer tray for easy cleaning

fan heater sensor kit

PLATE RACKS

FlexiCaster for Large Format Vertical

DNA Sequencing, 2-D analysis, Micro-satellite analysis, DNA fingerprinting, Gel shift assays, Single-Strand Conformation Polymorphism (SSCP), Heteroduplex and Oligonucleotide analysis

These sturdy racks are designed for safe drying and storage of glass plates. The small rack can hold up to 20x 2mm thick plates while the larger rack can accommodate up to 10x 5mm thick glass plates.

BSQ20, 20 x 50cm Large Format Vertical

ORDERING INFORMATION BCSQ20 CSQ20-NG CSQ20-PG CSQ20-S0.25 BCSQ33 CSQ33-NG CSQ33-PG CSQ33-S0.25 CSQ33-S0.35 MGPR

Large Format Vertical, 20cm wide, glass plates, 0.35mm spacers, 48 sample comb Glass plates, pk/2 Notched CSQ20-S0.35 Glass plates, pk/2 CSQ20-S1 Spacer set 0.25mm CSQ20-S1.5 Large Format Vertical, 33cm wide, glass plates, 0.35mm spacers, 48 sample comb Glass plates, pk/2 Notched CSQ33-S1 Glass plates, pk/2 CSQ33-S1.5 Spacer set 0.25mm FHS Spacer set 0.35mm CSQ-FC Mini Glass Plate Rack for 20x 2mm Plates LGPR

CODE

DESCRIPTION

CSQ20-0.25-24 CSQ20-0.25-48 CSQ33-0.25-48 CSQ33-0.25-96 CSQ20-0.35-24 CSQ20-0.35-48 CSQ33-0.35-48 CSQ33-0.35-96

Comb 24 sample, 0.25mm thick, Sharks tooth Comb 48 sample, 0.25mm thick, Sharks tooth Comb 48 sample, 0.25mm thick, Sharks tooth Comb 96 sample, 0.25mm thick, Sharks tooth Comb 24 sample, 0.35mm thick, Sharks tooth Comb 48 sample, 0.35mm thick, Sharks tooth Comb 48 sample, 0.35mm thick, Sharks tooth Comb 96 sample, 0.35mm thick, Sharks tooth

For 110 V AC, please add ‘-$’ as a suffix to the above code

SAMPLE VOLUME PER wELL

7µl 3µl 7µl 3µl 9µl 5µl 9µl 5µl

Spacer set 0.35mm Spacer set 1mm Spacer set 1.5mm Spacer set 1mm Spacer set 1.5mm Fan heater sensor kit for large format vertical units BCSQ20 and BCSQ33 FlexiCaster for large format vertical units BCSQ20 and BCSQ33 Large Glass Plate Rack for 10x 5mm Plates

CODE

DESCRIPTION

CSQ20-1-24 CSQ20-1-48 CSQ33-1-48 CSQ33-1-80 CSQ20-1.5-24 CSQ20-1.5-48 CSQ33-1.5-48 CSQ33-1.5-80

Comb 24 sample, 1mm thick, Square tooth Comb 48 sample, 1mm thick, Square tooth Comb 48 sample, 1mm thick, Square tooth Comb 80 sample, 1mm thick, Square tooth Comb 24 sample, 1.5mm thick, Square tooth Comb 48 sample, 1.5mm thick, Square tooth Comb 48 sample, 1.5mm thick, Square tooth Comb 80 sample, 1.5mm thick, Square tooth

SAMPLE VOLUME PER wELL

40µl 20µl 35µl 20µl 60µl 30µl 50µl 30µl

39

isoelectric focusing • FOR IPG • LARGE

STRIPS AND

IEF

GELS

COOLING PLATFORM AREA

• ‘PICk-AND-PLACE’

ADjUSTABLE

ELECTRODES

• FOCUSING TRAy IPG STRIPS • REHyDRATION

FOR A MAXIMUM TwELVE

TRAy ALSO INCLUDED

Typical Running Conditions 7CM IPG STRIP IEF STEP

1

2

3

4

5

6

VOLTAGE (V)

150

300

600

1500

3000

300

TIME (H)

0.5

0.5

0.5

0.5

2.5

<20

VOLT-HOURS

75

150

300

750

7500

-

1

2

3

4

5

VOLTAGE (V)

300

600

1500

3000

300

TIME (H)

0.5

1

1

12

<20

VOLT-HOURS

150

600

18CM IPG STRIP IEF STEP

1500 36000

-

IPG Strip Length Tray Specifications 7cm 11cm* 18cm 24cm FOCUSING TRAY ELECTRODE DISTANCE

6.5CM

MAXIMUM STRIP LENGTH ACCOMMODATED

25.3CM 25.3CM 25.3CM 25.3CM

IPG 7CM STRIP LENGTH

10.2CM 17.1CM 22.7CM

N/A*

18CM

24CM

MAXIMUM STRIP LENGTH ACCOMMODATED

24CM

24CM

24CM

24CM

RECOMMENDED VOLUME FOR STRIP REHYDRATION

3.5ML

6ML

8.0ML

12.0ML

Now equipped with rehydration and focusing trays, the redesigned Biocom IEF has been optimised to perform first-dimension isoelectric focusing (IEF) with IPG (immobilised pH gradient) strips quickly, easily and reproducibly. An ideal entry-level system for both inexperienced and occasional IEF users, the unit is also versatile enough to meet the needs of laboratories with increased throughput requirements.

Its high-capacity focusing tray accommodates up to twelve IPG strips. An Adjustable ‘pick-and-place’ electrodes clip conveniently anywhere within the focusing tray to resolve IPG strips 7-24cm in length; colour-coded to prevent polarity reversal. The Electrode frame clips directly on to the cooling plate and includes adjustable electrodes to run horizontal precast IEF and PAGE gels.

A cooling plate, manufactured from a special grade ceramic in a large 26 x 26cm surface area, facilitates effective heat dissipation and homogenous thermal control, particularly during high High specification voltage IEF techniques. An Chiller for use optional, but recommended, with flatbed recirculating chiller connects IEF unit, see page 18 quickly and easily to the cooling plate via snap-lock connectors to maintain

optimal operating temperatures for IPG strips (20°C) and precast gels (4°C).

The Rehydration tray allows convenient transfer of IPG strips to the focusing tray without time-consuming removal of residual rehydration buffer; also enables focusing tray to remain permanently in use for IEF to maximise throughput, and provides useful storage at -20°C for focused strips before second-dimension runs.

The Consort EV233 power supply, (3000V, 300mA, 300W) enables desired Volt-hours for focusing to be attained faster at maximum voltage. Its nine different program settings with nine different parameters and voltage ramping make the EV233 ideal for multi-step IEF techniques. Data-logging capacity for up to 3600 output values, while run data may be collected via RS232 connection and free downloadable data acquisition software.

REHYDRATION TRAY

Technical Specifications

5

MAX. COMMERCIAL STRIP LENGTH ACCOMMODATED 24CM MAX. GEL DIMENSIONS ON COOLING PLATE

26 X 26CM

UNIT DIMENSIONS (WXDXH)

55 X 35 X 10CM

FOCUSING TRAY STRIP CAPACITY

12X 18 AND 24CM STRIPS; 24X 7 AND 11CM STRIPS

OPERATING TEMPERATURE

4 - 25°C

2. Adjustable ‘pick-and-place’ electrodes

REGULATORY CERTIFICATION

CE, EN61010

3. Ceramic cooling plate with snap-lock connectors

Typical Applications 1st dimension of 2-D electrophoresis separation of proteins according to their PI

1

IEF COMPONENTS 3

1. Focusing Tray

4. Rehydration tray (not shown) 5. Electrode frame for horizontal precast gels 6. Shrouded 2mm high voltage cables (not shown)

ORDERING INFORMATION BCIEF

Flatbed IEF system for IPG strips and gels, with focusing and rehydration trays

BCCHILL IEF-KIT IEFPOS IEFNEG IEFPLT

Chiller system, -20 to 100°C, including insulated tubing & clips 1-D Combination Package, includes IEF, CHILLER and EV232 Replacement positive electrode Replacement negative electrode Replacement Glass platform

40

IEFFRME RHYDTRY FOCUSTRAY FTELECPOS EV233

Replacement electrode frame Rehydration Tray Focusing tray with adjustable electrodes Focusing Tray Adjustable Electrode Positive Consort 3000V, 300mA, 300W power supply

2

SECOND STAGE

2-D

electrophoresis

The omniPAGE Maxi Plus unit provides a convenient solution for the second stage of 2-D electrophoresis. The 26cm active gel width provides a large gel area to resolve large IEF strips. In combination with the IEF system above, this offers a complete package for 2-D electrophoresis.

The unit utilises the omniPAGE advanced design features to provide convenient ease of use with high resolution separations.

• IDEAL

FOR SECOND DIMENSION

ELECTROPHORESIS

• ACCEPTS

STRIPS UP TO

IN LENGTH

• RAPID

26CM

SET UP COOLPACkS FOR

ENHANCED RESOLUTION

Rapid set up cooling retains resolution in extended separations and also saves on buffer volume without affecting run quality.

Up to four 1mm thick gels can be resolved per run. A wide range of accessories is available to allow easy transition between 2-D and standard vertical electrophoresis techniques. Different types of 2-D comb allow a wide degree of versatility in sample selection and gel set-up.

Technical Specifications UNIT DIMENSIONS

36 X 33 X 18CM (L X W X D)

PLATE DIMENSIONS 30 X 22CM (W X L) GEL DIMENSIONS

28 X 20CM (W X L)

MAX SAMPLE CAPACITY

300 SAMPLES PER RUN; 75 SAMPLES PER GEL

BUFFER VOLUME

MIN 1800ML, MAX 8400ML

COMBS AVAILABLE NO. OF TEETH THICKNESSES

1, 2, 4, 28MC, 56MC, 75 0.25, 0.35, 0.5, 1, 1.5, 2.0MM

BCVS30D BCVS30DSYS VS30BI VS30DCAST VS30DCASTM BCVS30DIRM VS30ICB VS30-x -LG VS30NG VS30PG VS30NGS0.75 VS30PGS0.75

omniPAGE Maxi Plus, 30 x 22cm Dual with Glass Plates with bonded 1.5mm spacers, 2 x 28 sample combs, 2 x 2-D combs, cooling pack, dummy plate omniPAGE Maxi Plus, as above with Casting Base omniPAGE Maxi Plus Blot Insert - includes 3 cassettes and 6 fibre pads VS30NGS1 30 x 22cm Notched Glass Plates with 1mm Bonded Spacers (pk/2) 30 x 22cm Dual Casting Base VS30PGS1 30 x 22cm Plain Glass Plates with 1mm Bonded Spacers (pk/2) Replacement Silicone Mat for 30 x 22cm Casting Base VS30PGS1.5 30 x 22cm Plain Glass Plates with 1.5mm Bonded Spacers (pk/2) Inner Running Module VS30PGS2 30 x 22cm Plain Glass Plates with 2mm Bonded Spacers (pk/2) Maxi Cooling Pack VS30DP Dummy Plate, 20 x 20cm Loading guides for omniPAGE maxi combs, x = comb well number VS30S0.75 22cm Spacers - 0.75mm (pk/2) 30 x 22cm Notched Glass Plates 4mm thick (pk/2) VS30S1 22cm Spacers - 1mm thick (pk/2) 30 x 22cm Plain Glass Plates 4mm thick (pk/2) VS30S1.5 22cm Spacers - 1.5mm thick (pk/2) 30 x 22cm Notched Glass Plates with 0.75mm Bonded Spacers (pk/2) VS30S2 22cm Spacers - 2mm thick (pk/2) 30 x 22cm Plain Glass Plates with 0.75mm Bonded Spacers (pk/2) RPW-0.2100 Replacement Platinum Wire - 0.2mm, 100cm

Code

DESCRIPTION

VS30-1-1 VS30-2-1 VS30-4-1 VS30-28-1MC VS30-56-1MC VS30-75-1MC

Comb 1 Prep, 1 Marker, 1mm thick Comb 2 sample, 1mm thick Comb 4 sample, 1mm thick Comb 28 sample, 1mm thick MC compatible Comb 56 sample, 1mm thick MC compatible Comb 75 sample, 1mm thick MC compatible

SAMPLE VOLUME PER wELL

2250µl 1125µl 550µl 80µl 40µl 25µl

COMBS ALSO AVAILABLE IN OTHER THICKNESSES AND SAMPLE NUMBER, PLEASE INQUIRE

advance gel casting An additional External Casting Stand, available with or without base, offers a cost-effective method of advance gel casting. Based on the Inner Running Modules of omniPAGE MINI, MINI WIDE and WAVE MAXI, the unit is identical to the standard module but without the platinum wiring. Simply cast your gels as normal, allow to set, load samples and then transfer to the appropriate vertical Running Module.

Colour

Colour

ORDERING INFORMATION

SAMPLE VOLUME

CODE

DESCRIPTION

VS30-1-1.5 VS30-2-1.5 VS30-4-1.5 VS30-28-1.5MC VS30-56-1.5MC VS30-75-1.5MC

Comb 1 Prep, 1 Marker, 1.5mm thick Comb 2 sample, 1.5mm thick Comb 4 sample, 1.5mm thick Comb 28 sample, 1.5mm thick MC compatible Comb 56 sample, 1.5mm thick MC compatible Comb 75 sample, 1.5mm thick MC compatible

PER wELL

3375µl 1680µl 825µl 120µl 60µl 37µl

MC = multichannel pipette compatible

• LEAk

PROOF SEALS

• EASy

TO USE

• COMPATIBLE

wITH OMNIPAGE

GEL CHAMBERS

ORDERING INFORMATION VS10EXCASTER External Casting Stand - Upstand only VS10EXCASTERSYS External Casting System - Upstand + Casting Base

VS20EXCSTR External Casting Stand - Upstand only VS20EXCSTRSYS External Casting System - Upstand + Casting Base

41

denaturing gradient • MAXIMUM 96-SAMPLE

THROUGHPUT COMPATIBLE wITH MICROPLATES AND THERMAL CyCLER BLOCkS

• FOUR-SCREw

VERTICAL

CLAMPING TECHNOLOGy ACCELERATES SET UP

• LARGE

FORMAT

- 20X20CM

GLASS PLATES FOR IMPROVED

RESOLUTION

• 100ML GRADIENT MIXER, wITH VALVE-CONTROLLED 50ML RESERVOIR AND MIXING

CHAMBERS, MAkES TwO

PARALLEL DENATURING

1MM

GRADIENT GELS

• MICROPROCESSOR-

CONTROLLED TEMPERATURE CONTROL UNIT ACCURATE TO

±0.02°C

The newly designed WAVE-DGGE is a complete system for DNA mutation analysis. Using the innovative vertical screw-clamp technology of the new WAVE Maxi system, the WAVE-DGGE is fully equipped – with temperature control unit, stirrer, gradient mixer and casting accessories – to perform specific mutation analysis applications. The powerful microprocessor-controlled PID temperature control unit enables various mutation detection techniques to be undertaken between ambient temperature and 70°C, while the simple four-screw design of the WAVE module accelerates set up of denaturing PAGE gels. Accordingly, the new WAVE-DGGE can be used to screen singlebase pair changes in the following applications: • Heteroduplex analysis (HA) • Parallel Denaturing Gradient Gel Electrophoresis (DGGE) • Constant Denaturing Gradient Gel Electrophoresis (CDGE)

For combs, see page 35 Typical Applications Ideal for rapid checking of very large numbers of samples from PCR and cloning. High throughput Electrophoresis.

The flexibility provided by the modular design of the WAVE-DGGE and its wide range of accessories enables laboratories to switch quickly and easily between different mutation detection techniques, thereby maximising throughput and screening efficiency. A maximum 96-sample throughput allows detection of as many mutations within a couple hours, alleviating many of the bottlenecks associated with screening for DNA mutations.

GM100 gradient mixer

low-density denaturant solutions. A flat-base design and support handle allows the GM100 to be secured to a retort stand, enabling it to be easily mounted on a magnetic stirring plate, while the mixing chamber can accommodate a magnetic stirrer to form a linear gradient. The MU-D01 peristaltic pump is also recommended for delivery of linear and reproducible gradient gels.

The GM100 gradient mixer is supplied as standard to ensure efficient gradient formation by mixing and delivering high- and

WAVE-DGGE - Applications

Description

VS20WAVE DGGE Application Benefits

42

DGGE

CGGE

HA

i. Determines the denaturing conditions required to identify unknown mutations ii. Works on the principle that increasing denaturant concentrations melt DNA in a domain-specific manner, and the mutation or polymorphism of interest is in the DNA domain with the lowest Tm iii. Requires parallel DGGE – a technique where DNA samples are resolved at uniform temperature in gels containing a formamide and urea denaturant gradient parallel to the direction of electrophoresis iv. Results in partial melting of DNA to produce a branched molecule identified by its reduced mobility within the gel.

i. Rapid screening method for multiple samples containing an identified mutation ii. Requires DGGE beforehand to establish optimal denaturing conditions to identify a specific mutation iii. No denaturant gradient required as multiple samples are screened on a constant denaturant gel iv. Increases throughput and alleviates bottlenecks

i. Used when it is difficult to detect a homoduplex mutation by DGGE ii. Requires denaturation and re-annealing of wild type and mutant DNA mixed together, usually within a PCR reaction iii. Resultant heteroduplexes are less stable and melt at a lower denaturant concentration than wild-type and mutant homoduplex molecules, allowing them to be identified by reduced mobility within the gel iv. Requires parallel DGGE, or may be performed overnight in a TBE gel made from special high resolution acrylamide

i. GM100 gradient mixer and optional MU-D01 peristaltic pump simplify casting of denaturing gradient gels ii. New WAVE Maxi electrophoresis insert and cam caster for leak free casting iii. Temperature control unit provides consistent run temperatures between 45-70°C iv. High resolution 20x20cm format

i. Uses constant denaturant gels cast with new WAVE Maxi electrophoresis insert and cam caster for leak free casting ii. Temperature control unit provides constant run temperature during electrophoresis iii. Maximum 96-sample throughput (48 samples per gel)

i. New WAVE Maxi electrophoresis insert and cam caster for leak free casting ii. Gradient mixer simplifies DGGE option iii. Optional temperature control for reproducibility iv. High resolution 20x20cm format

DEnATuRInG GRADIEnT

WAVE-DGGE Innovative Casting and Set-up Mechanism The WAVE-DGGE utilises novel vertical screw clamp technology to assemble two vertical gels. This reduces the number of screws required for set up making casting assembly faster, while a built-in inner buffer chamber within the PAGE insert allows set-up to be completed without the inclusion of heavy top tanks or buffer chambers. A dual purpose PAGE module eliminates the need for plate transfer, and is used with a cam casting base to guarantee efficient leak free casting.

WAVE electrophoresis insert and cam casting base

Pre-electrophoresis: mutations are best detected when the single-base pair mutation itself is located within lowest melting domain of the DNA of interest, and the molecule does not denature entirely.

GC-clamping at one end of the DNA molecule ensures that the region to be screened is in the lower melting temperature domain while the DNA molecule remains partly double-stranded.

Recommended free POLAND analysis software (G Steger, University of Dusseldorf) predicts the melting behaviour of the DNA fragment of interest and resulting primer placement and GC clamp positioning.

Precise thermal control The redesigned WAVE DGGE-TC temperature control unit combines buffer recirculation with a heat sensor and 1.4kW heating element to facilitate precise temperature control to within ±0.02°C, allowing the gel temperature to be set to the melting temperature (Tm) of the amplified DNA polymorphism or mutation of interest. Other benefits include: a conspicuous 4-digit 16mm LED panel to aid set-up; precise tuning to within 0.1°C resolution; an operating set point, plus three adjustable pre-set temperature values; and stirred buffer circulation for temperature stability and uniformity.

Programmable power supply option At 500V, 800mA and 300W outputs, the optional MP-500 power supply provides full flexibility for different mutation detection techniques.

STIR Magnetic Stirrer, see page 57

MP-500V power supply, see page 49

Software options: 3 packages

Temperature control unit

Post electrophoresis: CLIQS is available for band pattern matching following parallel DGGE in a single gel; CLIQS 1D Pro allows comparison between multiple gels and different experiments.

MU-D01 Peristaltic Pump

Technical Specifications WAVE electrophoresis insert and tank Max. Number of Gels Plate Dimensions (W x H) Active Gel Dimensions (W x H) Spacer Thicknesses Buffer Max. Sample Capacity Standard Combs Available Combs Max. Buffer Volume Unit Dimensions (W x D x H) Weight

2 per run 20x20cm 16 x 17.5cm 0.75, 1, 1.5 and 2mm 96 samples; 48 per gel 2x 1mm 24-sample 1, 5, 10, 18MC, 24, 36MC, 48; as per WAVE Maxi 8.5L 40.5 x 17 x 44cm 8kg

Temperature Control Operating Temperature Range Working Temperature Range (DGGE) Buffer Recirculation Mechanism Temperature Uniformity/Stability at 37°C Setting/Display Resolution Safety

PID Ambient to 100°C 45-70°C Stirring ±0.05/0.02°C 0.1°C Fluid-level float switch; isolated; IEC 1010 / CE

Stored Temperature Values Heater Power at 230V/110VAC Electrical Power at 230V/100VAC

4 1.4/1.3kW 1.5/1.4kW

Gradient Mixer

Recommended Power Supply 500V Total 800mA 300W

Voltage Current Power

Temperature Control Unit

Total Volume Volume of Reservoir & Mixing Chambers Internal Diameter of Outlet Port

100ml 50ml 2mm

ORDERING INFORMATION

Complete Denaturing Gradient Gel Electrophoresis System, 20x20cm; includes: temperature control unit, cam casting base, glass plates with 1mm bonded spacers, 2x 24-sample combs and gradient mixer – 240 VAC version WAVE-DGGETC Temperature Control Unit GM100 Gradient Mixer, 100ml WAVE-DGGEKIT WAVE-DGGE Package; includes WAVE-DGGE, STIR, PP01, MU-S16, MP-500 WAVE-DGGE

STIR MU-D01 MU-S16 MP-500V

Magnetic Stirrer, 19 x 19cm Single Peristaltic Pump Silicon tube I.D. 1/8”, 25 ft (for peristaltic pump PowerPoint Power Supply, 500V, 800mA, 300W

CLIQS CLIQS 1D Pro

1D image analysis with band pattern matching 1D image analysis with band pattern matching between different gels

43

• IDEAL FOR wET ELECTROBLOTTING

OF PROTEINS - wESTERN BLOTTING

• UP TO FIVE GEL BLOT CASSETTES UTILISED AT ANy ONE TIME

• HINGED CASSETTES FOR ADDED CONVENIENCE

• ACCOMMODATES GEL THICkNESSES FROM

0.25 UP TO 3MM

EBLOT ELECTROBLOTTERS

blotting

Technical Specifications UNIT DIMENSIONS (W X D X H)

MINI 19 X 13 X 19CM MAXI 24 X 16 X 26CM

MAX. SAMPLE,

MINI 5 BLOTS, 10 X 10CM MAXI 5 BLOTS, 20 X 20CM 20 BLOTS, 10 X 10CM

CAPACITY

BUFFER VOLUME

Designed primarily for wet electroblotting of proteins, EBLOT Electroblotters offer a combination of increased capacity with economy saving features. Both units, Mini 10 x 10cm and Maxi 20 x 20cm, have increased capacity over standard systems with up to five gel blot cassettes utilised at any one time. This is especially useful in high throughput laboratories.

A uniform electric field is provided by a high intensity coiled electrode and ensures uniform transfer across the blot surface. The cassette’s open architecture ensures the maximum blot area allows direct transfer of current.

Its rigid construction ensures contact between the gel and membrane is retained throughout the blot and an even pressure is maintained. These units are compatible with magnetic stirrers to aid heat dispersal and prevent pH drifts in the buffer due to incomplete buffer mixing.

Each system includes a cooling pack to further enhance transfer efficiency by removing excess heat. This also saves on buffer for added economy. double hinged cassettes for added convenience

For omniPAGE blotters, see pages 31, 33 and 37

MINI MIN 1000ML; MAX 1500ML MAXI MIN 4300ML; MAX 6000ML

• LOw COST

DOT & SLOT

• EASy ASSEMBLy • FOUR SAMPLE CONFIGURATIONS • ALPHA-NUMERIC SAMPLE IDENTIFICATION

Model

D48

D96

S24

S48

Configuration

3 x 16

8 x 12

2 x 12

3 x 16

Size of well

6mm diameter 12mm deep

6mm diameter 12mm deep

6 x 0.5mm 12mm deep

6 x 0.5mm 12mm deep

Vacuum required Unit dimensions Membranes size required

----- 600mg Hg 0.8 BAR with cold trap ----60 x 95 x 100cm

60 x 105 x 140cm

60 x 74 x 83cm

60 x 95 x 100cm

12.1 x 4.4cm

11 x 7.4cm

12.1 x 4.4cm

12.1 x 4.4cm

ORDERING INFORMATION BCEBM10 BCEBM20 SB10C SB10F

44

EBLOT Mini ElectroBlotter, 10 x 10cm System for five cassettes, with tank

and lid, 5x cassettes, 12x fibre pads and cooling pack EBLOT Maxi ElectroBlotter, 20 x 20cm System for five cassettes, with tank and lid, 5x cassettes, 12x fibre pads and cooling pack SB20C EBLOT Maxi Cassette EBLOT Mini Cassette SB20F Fibre pads - pk/8 Fibre pads - pk/8

Dot Blot and Slot Blot microfiltration manifolds are designed for DNA and RNA filter blot hybridisations and immunological (Ag/Ab) screening applications. Machined from high density acrylic, their precision lapped mating surfaces and leak proof gasket ensures uniform filter contact, so preventing lateral transfer of samples – smudging – by ensuring that a complete vacuum is formed.

Assembly of the manifolds is simply by aligning the mating surfaces and tightening the stainless steel thumb screws. A permanent filter template is provided with each manifold to simplify the cutting of filters to the exact size. A vacuum of approximately 600mm Hg (0.8 Bar) is required during sample application.

Four configurations are available for 24 & 48 for slots and 48 & 96 wells for dots in the configuration of standard microplates. Each well is alpha-numerically grid referenced for easy identification.

Dot & Slot Blotters BCD48 48-well Dot Blot Manifold, 3 x 16 array BCD96 96-well Dot Blot Manifold, 8 x 12 array BCS24 24-well Slot Blot Manifold, 2 x 12 array BCS48 48-well Slot Blot Manifold, 3 x 16 array

semi-DRY

blotters These Semi Dry Blotters offer rapid transfer times for DNA, RNA and protein blotting - typically 15 to 30 minutes. All units can be used for all types of blotting: western,

Note: New injection moulded BCSD10/BCSD20 style shown; other models are of different construction

southern and northern via uncomplicated buffer and set up procedures and are compatible with gel thicknesses from 0.25 up to 10mm without the need for additional equipment. Each unit is compatible with their respective omniPAGE vertical gel system.

NEW

• FIVE

SIzES

DUE TO

HEAT DISPERSION

Technical Specifications UNIT DIMENSIONS MINI MAXI (W X L X H) MAXI PLUS MAXI LONG

16 X 16 X 7CM 26 X 26 X 7CM 39 X 51 X 7CM 26 X 56 X 7CM

MAX. SAMPLE,

MAXI PLUS MAXI LONG

1 BLOT, 8 X 8.5CM 1 BLOT, 16 X 17.5CM 2 BLOTS, 16 X 8.5CM 4 BLOTS, 8 X 8.5CM 1 BLOT, 35 X 45CM 1 BLOT, 20 X 50CM

MINI MAXI MAXI PLUS MAXI LONG

5ML 20ML 75ML 50ML

CAPACITY

BUFFER VOLUME

MULTIPLE

GRADIENTS

AND

• ECONOMIC TRANSFERS

• UNIFORM

gradient mixers OF CENTRIFUGATION

• wESTERN, SOUTHERN NORTHERN BLOTS

• SCREw DOwN LID - ACCOMMODATES GELS FROM 0.25 UP TO 10MM

Uniform heat dispersion across the blot sandwich ensures stable transfer times and no heat induced sample loss or transfer distortions. Being translucent, it allows viewing of the blot sandwich to ensure correct positioning and transfer is occurring correctly.

Electrode plates are fully separated to prevent arching or damage.

PREPARATION

TRANSFER TIMES

VERy LOw BUFFER VOLUMES

Semi Dry Blotting has the added benefit of economic transfers due to very low buffer volumes – typically only a few millilitres of buffer are required per transfer. These Semi-Dry Blotters utilise a screw down lid, which secures the blot sandwich and allows complete control of pressure ensuring even transfer. The electrodes, comprising platinum coated anode and stainless steel cathode, will exhibit practically no corrosion and so provide many years of trouble free use.

• FOR

• RAPID

MINI MAXI

gel casting • INTEGRAL

LEVELLING FEET AND LEVEL BUBBLE

• STANDARD

AND GRADIENT GELS CAN BE POURED

• CHOICE

OF 6, 12 OR CASTING MODELS

24

GEL

Ideal for Caesium, Sucrose and Gel gradients the Gradient Mixer series comprises two chambers a reservoir and a mixing chamber with an interconnecting valve. A second valve regulates the output flow from the mixing chamber. All mixers have a flat base which allows them to be placed on a magnetic stirrer. A magnetic stirring bar can be placed directly in the mixing chamber to ensure a constant gradient. The support rod allows the mixer to be fixed to a retort stand for extra stability.

Advance casting of multiple mini and maxi gels can help to achieve consistent results between runs. These multiple gel casting systems are tailored for use with omniPAGE mini and omniPAGE maxi vertical electrophoresis units; with three models of 6, 12 and 24 gel capacities. Fewer gels can be poured if required using the acrylic saver blocks supplied with each system.

The fixed hinged clamps allows the gel sandwich to be adjusted to the correct pressure irrespective of the number or the thickness of gels being poured. Separation sheets allow the easy separation of the glass plates after pouring.

ORDERING INFORMATION ORDERING INFORMATION

Semi Dry Blotters* VS20D omniPAGE Maxi, 20 x 20cm Dual with Glass Plates with bonded 1mm thick spacers, 2x 24 sample combs, cooling pack, dummy plate SD10 Semi Dry Mini, 10 x 10cm System SD33* Semi Dry Maxi Plus, 33 x 45cm System VS20DSYS omniPAGE Maxi, 20 x 20cm Dual with Glass Plates with bonded 1mm thick spacers, 2x 24 sample combs, cooling pack, dummy plate and Casting Base SD20 Semi Dry Midi, 20 x 20cm System, shown above SD50* Semi Dry Maxi Long, 20 x 50cm System Gradient Mixers GM15 15ml Gradient Mixer GM25 25ml Gradient Mixer GM50 50ml Gradient Mixer Multi Vertical Gel Casters 6CAST 6 gel caster for 8 x 10cm or 10 x 10cm mini gels 12CAST 12 gel caster for 8 x 10cm or 10 x 10cm mini gels * Note that large format Semi Dry Blotters are fabricated rather than injection moulded

GM100 GM500

100ml Gradient Mixer 500ml Gradient Mixer

24CAST 206CAST

24 gel caster for 8 x 10cm or 10 x 10cm mini gels 6 gel caster for 20 x 20cm gels maxi gels

45

buffers, markers, membranes • CONVENIENT,

PRE-MADE

STOCk SOLUTION OR POwDER

–

jUST DILUTE

OR DISSOLVE AS NECESSARy wITH wATER

• SAVE

TIME

&

TROUBLE

NO wEIGHING, PH

–

ADjUSTMENT OR NEED TO STOCk INDIVIDUAL

PAGE buffers Five buffers are available in powder sachets for a range of native and denaturing protein gel electrophoresis techniques. Each powder sachet, which is supplied as a 10pack, may be reconstituted to make 1 litre of working solution. Running buffers are also available in 1 litre and 5 litre volumes as ready made 10x Tris-Glycine and 10x Tris-Glycine-SDS solutions.

COMPOUNDS

• LONG

SHELF-LIFE

• CONSISTENCy ASSURED – RIGOROUS qC FOR

Technical Specifications Powder Buffer Tris-Glycine SDS

Composition Each litre of 1x working solution contains: Tris-base (25mM); glycine (192mM); SDS, 0.1% (w/v); followed by distilled water. Working solution pH = 8.3.

Applications Denaturing SDS-PAGE for most cellular proteins, 10- 200kDa in size

Tris-Glycine

Each litre of 1x working solution contains: Tris-base (25mM); glycine (192mM); followed by distilled water.

Working solution pH = 8.3.

Native PAGE

Tris-Tricine-SDS

Each litre of 1x working solution contains: Tris-base (0.1M); tricine, (0.1M); SDS, 0.1% (w/v); followed by distilled water. Working solution pH = 8.25.

Denaturing SDS-PAGE, with greater resolving power for small proteins 2-20kDa in size

MOPS-SDS

Each litre of 1x working solution contains: MOPS (50mM); Tris Base (50mM); SDS, 0.1% (w/v); EDTA (1mM); followed by distilled water. Working solution pH = 7.7.

Denaturing SDS-PAGE for medium to large-sized proteins

MES-SDS

Each litre of 1x working solution contains: MES (50mM final stock concentration); Tris Base (50mM); SDS, 0.1% (w/v); EDTA (1mM); followed by distilled water. Working solution pH = 7.3.

Denaturing SDS-PAGE for small- to medium- sized proteins; faster than MOPS

REPRODUCIBLE SEPARATIONS

BP GRADE

ultra pure water Purified water (18 mega Ohms) for use with sensitive experimental procedures often needs verifying as pyrogen free, this is done using the LAL test or Limulus (Horseshoe crab) amoebocyte lysate assay.

The LAL test is extremely sensitive to endotoxins which are the result of bacterial lysis.

BP Grade Sterile Water has endotoxins removed by electrostatic filtration at the final purification stage prior to autoclaving. The LAL tested water conforms to the standard having less than <0.25EU/ml to ensure the water is of pre-requisite quality. This product is therefore pyrogen free. CFU>0 WFi compatible.

LAL (Limulus Amoebocyte Lysate Assay)

ORDERING INFORMATION

Powder Buffers TGSDSP Powdered Tris-Glycine-SDS Running buffer - 10 Sachets (10 litres/pk) TGP Powdered Tris-Glycine Running buffer - 10 Sachets (10 litres/pk) TTSDSP Powdered Tris-Tricine-SDS Running buffer - 10 Sachets (10 litres/pk) MSDSP Powdered MOPS-SDS buffer Running buffer - 10 Sachets (10 litres/pk) MESDSP Powdered MES-SDS buffer Running buffer - 10 Sachets (10 litres/pk)

Liquid Buffers TG10X1L TG10X5L TG-SDS10X1L TG-SDS10X5L

NEW

Ponceau S Ponceau S staining solution is reusable and available in a convenient 500ml volume for membrane staining and early protein detection following transfer before western blotting.

Ponceau S may also be supplied a powder staining kit for longterm storage.

Rnase-Free Water DEPC-treated to eliminate enzyme activity and then autoclaved, this sterile highly purified water product is perfect for use in PCR and Northern blotting techniques. RNase-free water is available either as a single 250ml bottle or in fifty 5ml aliquots to prevent cross-contamination.

Buffer Tris-Glycine 10 x 1 litre Buffer Tris-Glycine 10 x 5 litre Buffer Tris-Glycine SDS 10 x 1 litre Buffer Tris-Glycine SDS 10 x 5 litre

PSS

Ponceau S staining solution (500ml)

PSB

Ponceau S staining solution powder staining kit (makes 2000ml)

UPW1000 RFW250

BP Grade Sterile Water, 1000ml RNase-Free Water, 1x250ml

RFW50X5

RNase-Free Water, 50x5ml

46

protein markers • STANDARD (10-175kDA) AND wIDE (10-245kDA) MOLECULAR

Stable for up to 2 years if stored at -20°C and supplied pre-stained in gel loading buffer for direct loading, PINK Plus and BLUE Wide Range recombinant protein markers are perfect for SDS-PAGE applications. Sizes range from 10-175kDa for PINK Plus and 10-245kDa for BLUE Wide Range, making both markers suitable for accurate molecular weight determination of most cellular proteins.

wEIGHT RANGES AVAILABLE

• COLOURED

REFERENCE BANDS

FOR EASy IDENTIFICATION IN

GELS AND BLOTS

• STABLE

Each marker is covalently bound to a pink or blue colour chromaphore to produce a ladder of evenly interspersed bands of uniform intensity. Coloured reference bands serve as visual indicators of electrophoresis run progression and the efficiency of western transfer onto membranes following SDS-PAGE. Both PINK Plus and BLUE Wide Range markers can be detected at volumes as low as 2.5µl per well.

FOR

2

yEARS AT

-20°C

PPL Pink Plus

Technical Specifications Code Size Range Number of Bands Reference Bands Contents

Volume Supplied Storage Loading Volume Number of Applications Source

PPL BBL 10-175kDa 10-245kDa 11 12 10, 40 and 90kDa blue 25 & 75kDa; green & red Max. 2.2mg total protein in Max. 2.4mg total protein in 15% (v/v) glycerol, 2% SDS, 15% (v/v) glycerol, 2% SDS, 20mM Tris pH 7.5, 20mM Tris pH 7.5, 1mM 2-ME and 3.6M Urea 1mM 2-ME and 3.6M Urea 500µl 500µl 3 months at 4°C & 24 months at -20°C 2.5-5µl/well 100-200 Recombinant proteins, various sources

PVDF sheets & membrane rolls

BBL Blue Wide Range

NE W

Used in Western Blotting of Proteins and for use in Hybridisation Techniques. PDVF with nitrocellulose and nylon membranes are available for different application needs.

A protein’s properties (i.e., charge, hydrophobicity, etc.) affects its ability to bind to membrane surfaces. Finding the optimal membrane may require experimenting with a specific protein on different materials.

PVDF membrane is supplied in sheet form and as a 3M role which can be cut to size to fit a particular need.

blotting membrane rolls Supplied in 0.24x3m and 0.3x3m (w x l) sizes, allowing them to be cut to match specific gel formats, these membrane rolls are suitable for transfer of proteins and nucleic acids from polyacrylamide and agarose gels. Offered in 0.2μm and 0.45μm pore sizes.

blot-absorbent filter paper This blot-absorbent filter paper is supplied in packs of 50 and in sizes of 10x10cm and 20x20cm. Its 1mm thick texture and high buffer retention properties, being able to absorb twice its own weight in buffer, allow it to exert the gel-membrane compression needed for efficient transfers.

ORDERING INFORMATION PPL

Pink Plus Prestained Protein Ladder, 10-175kDa, with 10, 40 & 90kDa reference bands, 1x 500µl vial.

BBL

Blue Wide Range Prestained Protein Ladder, 10-245kDa, with 25 & 75kDa reference bands, 1x 500µl vial.

RNC45 RNC2

Nitrocellulose roll, 0.3x3m (w x l) Nitrocellulose roll, 0.3x3m (w x l)

RNY45 RNY2

Positively charged supported nylon, 0.24x3m (w x l) Positively charged supported nylon, 0.24x3m (w x l)

BP1010

Blot-Absorbent Filter paper, 10x10cm, pack of 50

BP2020

Blot-Absorbent Filter paper, 20x20cm, pack of 50

PVDF0.22S PVDF0.45S

10 Pre-cut PVDF 28 x28 cm 0.22µm 10 Pre-cut PVDF 28 x28 cm 0.45µm

PVDF0.22R PVDF0.45R

Roll PVDF 28 cm x 3 m, 0.22µm Roll PVDF 28 cm x 3 m, 0.45µm

47

OR POwER

• AUTOMATIC

VOLTAGE, CURRENT CROSSOVER

• OVERLOAD PROTECTION • SHORT CIRCUIT PROTECTION

Technical Specifications

Maxi T IMER

9 OF 9 SETS EACH

OUTPUTS

4 IN PARALLEL, 4MM SOCKETS

RESOLUTION

1V, 1MA, 1W

DIMENSIONS

31 X 26 X 15CM (W X L X H)

WEIGHT

10KG

Mini T

IMER

9 OF 9 SETS EACH

OUTPUTS

3 IN PARALLEL, 4MM SOCKETS

RESOLUTION

1V, 1MA, 1W

DIMENSIONS

14 X 20 X 13CM (W X L X H)

WEIGHT

3KG

Timer - can be set to automatically terminate the run and sound an alarm when the allocated time or volt hours has elapsed.

Automatic cross-over - each model has constant voltage, constant current and constant power capabilities with automatic cross-over while showing which parameter is kept constant.

Safety precautions - the AC line is automatically disconnected from the high voltage transformer when a ground leakage path is detected protecting the user from potential shock. Other safety features include protection against any overload including accidental short circuit of the output and voltage increases smoothly.

0 - 99.59 MINS

PROGRAMS

Programming - up to 9 different programs, each with 9 steps, can be stored in the non-volatile memory for future recall.

Data Transfer - optional data acquisition/control software for PC is available to visualise and examine the stored run details, store and program the methods, add notes to the run, identify the unit, copy or delete programs in the unit's memory.

Technical Specifications

Mini series

power supplies have smaller footprints and three output terminals. While the powerful microprocessor allows complex programming, the Manual mode permits the setting of voltage, current, power and time for a routine electrophoresis run. Parameters can be changed temporarily without interrupting the run.

Automatic recovery after mains failure - after restoration of power, the instrument will automatically continue the run for the remaining time.

0 - 99.59 MINS

PROGRAMS

Consort Maxi Series (EV200) Power Supplies except EV245 have four output terminals to allow four simultaneous runs. Model EV245 Mini

Maxi series

CONSORT MINI EV245

• CONSTANT

cONsORt pOweR sUppLIes

powerpoint

This Consort Mini power supply covers the ideal voltage range generally used with small electrophoresis units. Small enough to occupy very little bench space, they are portable and have a variety of additional features:Manual Set-up - voltage, current and power settings can be adjusted during the run Voltage ramp - this allows a linear voltage gradient for any step to be programmed Automatic recovery after power failure 3 output terminals 9 different programs, each with 9 steps; datalogging and data transfer to a PC, and PC remote control

ORDERING INFORMATION EV231 EV202 EV265 EV261

Consort Power Supply Consort Power Supply Consort Power Supply Consort Power Supply

300 V 300 V 600 V 600 V

1000 mA 2000 mA 500 mA 1000 mA

150 watts 300 watts 150 watts 300 watts

EV215 EV233 EV262 EV245

Consort Power Supply Consort Power Supply Consort Power Supply Consort Power Supply

1200 V 3000 V 6000 V 400 V

500 mA 300 mA 150 mA 500mA

300 watts 300 watts 300 watts 50 watts

For 110 V AC, please add ‘-$’ as a suffix to the appropriate product code

RAPID PRODUCT SELECTOR

NANO300 NANO500 MP-300 300V 400mA 60W

multiSUB Horizontal, all models COMET Assay Cellulose Acetate System omniPAGE Mini, Mini Wide omniPAGE WAVE Maxi Tube Gel IEF, Mini, Mini Wide & Maxi  Flatbed IEF  Second Stage 2-D Electroblotting, Mini, Mini Wide Electroblotting, Maxi  Semi-Dry Blotting, Mini Semi-Dry Blotting, Maxi  Large Format, 20 and 33cm wide

48

✔ ✔ ✔ ✔

500V 400mA 150W

✔ ✔ ✔ ✔ ✔

300V 700mA 120W



✔ ✔ ✔ ✔

✔

✔ ✔ Recommended

EV245

EV231

EV265

MP-500 MP-3AP

400V 300mA 50W

300V 1000mA 150W

600V 500mA 150W

500V 400mA 200W

300V 3000mA 300W

• •

• •

• •

• •

• •

•

•

✔ ✔ ✔ ✔

•

✔ •

✔ •

✔

EV202

•

✔

•

✔ ✔ ✔ ✔

EV261

300V 600V 2000mA 1000mA 300W 300W

• Can be used but not ideally matched 

✔ ✔ ✔ ✔

EV215

EV233

EV262

1200V 500mA 300W

3000V 150mA 300W

6000V 150mA 300W

• •

• •

✔ ✔

✔

•

•

•

✔ ✔

• •

✔

• •

• HIGH

PERFORMANCE, EASy TO OPERATE

POWERPOINT MAXis are completely programmable with self prompting menus and multiple operation modes.

• LARGE LCD

PARAMETERS

Versatile: two models are available offering maximum output capability to 500V, 400mA to 3A and 300 watts.

These units are suitable for running multiple SDS gels, blotting and large submarine gels.

MP-500 amd MP-3AP

Fully Programmable: system parameter entry is via the membrane keypad. They can be programmed to recall and run constant or multi-step electrophoresis in a variety of operational modes :

1. Constant operation with timer - program constant voltage, current or wattage with timer to eliminate the possibility of over running samples in gels.

2. Memory mode - Store 16 files to recall and re-run favourite multi step programmes

adapters BC4-4 – extends the length of standard electrode cables to fit within the deeply recessed power outputs of IEC 1010-1 compliant power supplies BC4-2 – allows systems to be run from high voltage power supplies with 2mm outputs BC2-4 – connects standard cables supplied with the BC-IEF and large format CSQ verticals to lowto-medium voltage power supplies – e.g. MP-500P

Display: POWERPOINT MAXi power supplies have large 2.6” LCD displays to show run/set parameters which will be shown continuously to allow comparison of set to actual run conditions. This can be viewed by touching ‘select display’ key.

OR POwER

• MEMORy

MODE - STORES 30 FILES TO RECALL AND RE-RUN

• COMPLIES

wITH ALL RELEVANT SAFETy STANDARDS

Technical Specifications CAT. NO. MAX. VOLTAGE MAX. CURRENT MAX. POWER OUTPUT TYPE

300V 3A 300W

---- 1-999 MINS ----

TIMER DISPLAY

-- 2.6” LCD DISPLAY SCREEN --

SAFETY

---- NO LOAD; LEAKAGE AND ---OVERLOAD DETECTION

OPERATION VOLTAGES

18 (W) X 28 (L) X 9.5 (H) CM 110 / 220V

Technical Specifications

Midi

MIDI POWERPOINT

MP-300

allows the unit to be set-up to run in constant voltage and constant current modes. Four gel chambers can be connected at any one time. A timer with alarm and pause functions can be set to end of run. Safety features include shrouded plugs and sockets, no load detection and ground leakage detection.

500V 800MA 300W

PROGRAM MULTI-STEP ---- UP TO 6 STEPS ----

CURRENT

This inexpensive, compact POWERPOINT MIDI power supply is ideal for routine horizontal and mini vertical electrophoresis. Microprocessor control

MP-3AP

PROGRAM STORAGE ---- 30 PROGRAMMED FILES ----

Safety: POWERPOINT MAXi models meet all relevant safety standards to protect both the operator and the instrument. The alarm will sound in event of ground leakage, no load or overload.

• PROGRAMME CONSTANT VOLTAGE AND • ALARM FOR END OF RUN • CONSTANT VOLTAGE OR CURRENT

MP-500P

CONSTANT VOLTAGES; CONSTANT CURRENT; OR CONSTANT POWER

DIMENSIONS

Recommended

DISPLAy OF RUN

• COMPLETELy PROGRAMMABLE • CONSTANT VOLTAGE, CURRENT

Maxi

MAxI pOweRpOINt

POWERPOINT MAXi Programmable Power Supplies are high performance, easy to operate instruments. Offering both graphic and text displays,

CAT. NO.

MP-300

MAX. VOLTAGE MAX. CURRENT MAX. POWER

300V 700MA 150W

OUTPUT TYPE

CONSTANT VOLTAGE OR CURRENT

SAFETY TIMER DIMENSIONS

---- NO LOAD; LEAKAGE AND ---OVERLOAD DETECTION ---- 1-999 MINS ---19 (W) X 29 (L) X 9.5 (H) CM

NANO300 and NANO500

• COMPACT • SAFETy DESIGN : SHROUDED NO LOAD DETECTION

PLUG AND SOCkET;

• CONSTANT VOLTAGE • LOw COST AND ECONOMICAL

POWERPOINT MINi power supplies have been developed specifically for horizontal gel electrophoresis systems. These ultra compact power units with dual outlets, are recognised as being amongst the most compact and inexpensive power supplies currently available. They are designed and constructed to the most rigorous safety standards.

110 / 220V

Technical Specifications

Mini

MINI POWERPOINT

OPERATION VOLTAGES

CAT. NO. MAX. VOLTAGE MAX. CURRENT MAX. POWER

NANO300

NANO500

300V 400MA 60W

500V 400MA 120W

OUTPUT TYPE

CONSTANT VOLTAGE OR CURRENT

SAFETY

---- NO LOAD DETECTION ----

DIMENSIONS

14 (W) X 19 (L) X 8.4 (H) CM

OPERATION VOLTAGE

110 / 220V

ORDERING INFORMATION NANO300 NANO500 MP-300 MP-500P MP-3AP

PowerPoint, NANO300 PowerPoint, NANO500 PowerPoint, MIDi 300 PowerPoint, MAXi 500 PowerPoint, MAXi 300

300V 500V 300V 500V 300V

400mA 400mA 700mA 800mA 3000mA

60W 120W 150W 300W 300W

BCAB BCAB2 PC4-4 BC4-2 BC2-4

Replacement Cables, pk/2 Replacement BCSQ unit cables, pk/2 Power supply adapters, 4mm to 4mm Power supply adapters, 4mm to 2mm Power supply adapters, 2mm to 4mm

49

Dryers • DRy UP TO TwELVE 10 X 10CM GELS AT A TIME [MAXI]

• MICROPROCESSOR

CONTROLS TEMPERATURE AND TIMER

• OPTIMAL

TIGHT SEAL DURING THE DRyING PROCESS

Vacuum Pumps • VACUUBRAND - OIL-FREE VACUUM DOwN TO ULTIMATE VACUUM

~ 9MBAR

VACUUM & HEATED

gel drying

• VACUUBRAND -

OUTSTANDING CHEMICAL RESISTANCE AND SUPERIOR VAPOUR TOLERANCE

With a drying area of 21 x 31 cm, the Midi Gel Dryer can dry six 10 x 10cm gels or a single larger gel. The Maxi Gel Dryer with a 35 x 45cm drying area can dry twelve 10 x 10cm mini gels simultaneously. Both unit’s microprocessor

Midi Gel Dryer

controls temperature and time, each parameter being displayed on its own LED display. The gels are heated from the base plate while the vacuum removes the moisture from below to dry the gel homogeneously. These dryers feature optimal sealing using a silicone rubber cover and supporting mask. When applying the vacuum, a groove that frames the drying surface provides an optimal tight seal during the drying.

Technical Specifications

Dryers T I

EMPERATURE NCREMENT

Maxi Gel Dryer

vacuum pumps

0.1°C

TEMPERATURE CALIBRATION YES TEMP UNIFORMITY

± 0.2°C

TIMER

1-999 MINS

DRYING AREA

MIDI MAXI

OPERATING TEMP. RANGE

AMBIENT TO 90°C

DIMENSION WXLXH

MIDI MAXI

The VacuuBrand® MZ 2C + 2 AK is a quiet, low maintenance oil free vacuum pump, which generates the lowest pressure measurable reproducibly at room temperature for almost 100% efficient solvent recovery. Two round bottomed flasks collect and condense noxious gases released during gel drying, whereas inlet and outlet separators act in tandem with the diaphragm pump to facilitate controlled collection of vapours without risk of condensate entering and contaminating the pump. The two stage pump also benefits from a compact design and construction from specially-selected chemically resistant materials, long-life easy-to-change diaphragms and valves, and gas ballast function.

21 X 31CM 35 X 45CM 30 X 36 X 8CM 44 X 50 X 8CM

Vacuubrand P F R 35L/ Pump UMP LOW ATE

PUMPING SPEED

MIN

1.9M3/H / 2.1 M3/H /.2CFM

ULTIMATE VACUUM (TOTAL) 9 MBAR // 6.8 TORR DIMENSIONS WXLXH

24 X 35 X 33CM

uv crosslinker

• PROGRAMMABLE

MICROPROCESSOR CONTROL

• AUTOMATIC MONITORING UV ENERGy • CONSPICUOUS FRONT LED, wITH NON-UV

OF

PANEL

TRANSMISSIBLE FRONT DOOR

CONNECTED TO SAFETy INTERLOCk SwITCHES

Technical Specifications UV SOURCE

5 X 8W UV BULBS, 254, 302 OR 365NM

EXPOSURE TIME

0 – 999.9 MINUTES

ENERGY RANGES

0 – 99T.99 J OR 0 – 9.999 J

INTERNAL DIMENSIONS

26 X 33 X 14.5CM (WXDXH)

FOOTPRINT

35 X 36CM

The UVLink UV Crosslinker is especially designed for binding nucleic acids to membranes. A membrane keypad facilitates manual or preset control of the desired UV dosage and exposure time, while a highly accurate microprocessorcontrolled photo feedback system maintains uniform output from each of the crosslinker’s five 8-Watt UV bulbs. Other features comprise safety interlock switches to prevent accidental UV leakage during operation, a clearly visible LED, plus a large interior chamber and small footprint area. The crosslinker may be used in a variety of applications, such as colony or plaque lifts, UV sterilisation and gene mapping or DNA damage studies.

ORDERING INFORMATION GDVH GDVH-35 MZ2C

50

Midi Gel Dryer, 21 x 31 cm Maxi Gel Dryer, 35 x 45 cm VacuuBrand Vacuum Pump, MZ 2C + 2 AK

CL-508.G CL-508.M CL-508.BL

Shortwave Crosslinker, 254nm Midrange Crosslinker, 302nm Longwave Crosslinker, 365nm For 110 V AC, please add ‘-$’ as a suffix to the appropriate product code

UV TRANSILLUMINATORS

gel visualisation These UV Transilluminators are multitasking workstations offering an

• THREE wAVELENGTH OPTIONS: 254 / 312 / 365 NM

ultra-violet source for the analysis of fluorescently stained DNA, RNA, and Protein electrophoresis gels. These also offer space to place tube racks, cutting tools or waste agarose gel, ideal when there is a need to cut gel bands.

• TwO DUAL wAVELENGTH MODELS • LONG

Each single wavelength model includes a High (100%) and Low (70%) intensity switch. A new and high quality starter ensures that the UV tubes energise immediately and without flickering. This feature extends the life of the tubes. Three Dual Wavelength models offer added flexibility and convenience.

LIFE FILTER

• TwO

SIzE OPTIONS

• HIGH

EFFICIENCy REFLECTOR

• HI / LO • FAST

The white light box allows imaging of nonfluorescent stained media, to allow a wider range of applications. Viewing Area: 210 x 297mm.

INTENSITy SwITCH

START UP

-

NO BLINk

Technical Specifications

Small F :

21 X 21 CM

ILTER SIZE

LIGHT SOURCE:

8W X 6 TUBES

UNIT DIMENSIONS (WXLXH): 34 X 29.5 X 10CM

Large F :

26 X 21 CM

ILTER SIZE

showing white light converter plate

LIGHT SOURCE:

8W X 8 TUBES

UNIT DIMENSIONS (WXLXH): 34 X 29.5 X 10CM

white light box

safeVIEW The new safeVIEW LED transilluminator offers a safe way to view and document samples. This light source also has the added advantage that it does not cause damage to DNA or RNA that would normally be associated with UV light.

• NO DNA

DAMAGE TO SAMPLES

• SAFER FOR USER - NO UV LIGHT • HIGH PURITy LED

LIGHT

• STRONG METAL ENCLOSURE wITH

Each safeVIEW Light Transilluminator is supplied as a standalone unit and can be used with microDOC, as part of a fully integrated gel documentation system. With a large surface area, each transilluminator serves as the perfect workstation for viewing and working with fluorescently-stained protein and nucleic acid gels.

STAINLESS STEEL FILTER FRAME

• FAST

START UP

Technical Specifications

NEW

FILTER SIZE

21 X 21CM

LIGHT SOURCE

470NM BLUE LED’S

SIZE

340 X 270 X 130MM

WEIGHT

5KG

VOLTAGE

110 - 240V (SELECTABLE)

ORDERING INFORMATION UVTS254 UVTS312 UVTS365 UVTSDUO UVTSDUO312 UVTSDUO365 UVTS254L UVTS312L UVTS365L

UV Transilluminator small, 21 x 21 cm, 254 nm UV Transilluminator small, 21 x 21 cm, 312 nm UV Transilluminator small, 21 x 21 cm, 365 nm UV Transilluminator small, 21 x 21 cm, 254/365 nm UV Transilluminator small, 21 x 21 cm, 254/312 nm UV Transilluminator small, 21 x 21 cm, 254/365 nm UV Transilluminator large, 26 x 21 cm, 254 nm UV Transilluminator large, 26 x 21 cm, 312 nm UV Transilluminator large, 26 x 21 cm, 365 nm

UVTLDUO UVTLDUO312 UVSCRN MDOCWLB Txxx

UV Transilluminator large, 26 x 21 cm, 254/365 nm UV Transilluminator large, 26 x 21 cm, 254/312 nm UV to white light Transilluminator screen converter White light box 8W UV bulb (xxx = 254 nm, 312 nm or 365 nm)

LEDTS UVPS22 UVPS27

safeVIEW BLUE Light Transilluminator, 21 x 21cm UV Transparent Cutting Platform 22 x 22 cm UV Transparent Cutting Platform 22 x 27 cm

51

Technical Specification

TotalLab

CLIQS 1D

CLIQS 1D Pro

Compatible with 21CFR part 11 compliance module

√

√

Automatic detection of lanes and bands

√

√

√

Automatic background subtraction

√

√

√

Image manipulation tools

√

√

√

√

Lane templates

√

Analysis protocols for batch processing

√ √

Molecular weight calibration

√

√

√

Quantity calibration & normalisation

√

√

√

Profile deconvolution

√ √

√

√

Rf calibration Band pattern matching – single gel

√

√

√

√

√

TOTALLAB 1D AND CLIQS

gel analysis software TotalLab 1D and CLIQS gel analysis software options are available for quantitative gel analysis following gel documentation. Each software option offers the highest level of automation currently available and guides the user step by step through the analysis process. A user-friendly interface is split into four parts allowing the user to view within a single screen every aspect of gel quantitation, including the gel image, lane and band profiles, analysis data and the help menu.

TotalLab 1D is used primarily for 1D gel quantitation and is suitable for all users regardless of their experience. More advanced CLIQS software is recommended for researchers performing in-depth lane relationship studies. Details of each software option are as follows:

TotalLab 1D Module, the 1D gel analysis module of CLIQS (see below), is supplied exclusively with microDOC1D models. TotalLab 1D features a user-friendly interface and help menu that provide a simple, guided workflow for fast and accurate quantitation and calibration of 1D gels and western blots. Main benefits include: • The capacity to review each step within the automated workflow analysis, and manually intervene or edit if desired

Band pattern matching – lanes across multiple gels

• Highly developed algorithms which accurately detect lanes and bands even on distorted gel images

Band pattern queries

• A range of visualisation tools that facilitate further examination of lane and band data to verify results, including band calibration from Molecular Size standard lanes and accurate quantitation derived from known band volumes

Dendrogram – single gel

√

√

√

Dendrogram – lanes from multiple gels Data archive and search facility Classification and identification tools √

Reports Array analysis module Colony counting module

√

√

√ √

Tool Box module

√

√

√

• Full user control over the visualisation tools and the data displayed, allowing only the important data fields and images of choice to be selected for final output • Multiplex analysis

√

TotalLab 1D

CLIQS includes TotalLab1D plus three modules for array analysis; colony counting and 2D spot measurement; and general feature-based image analysis. The array analysis module can automatically detect up to 1536 wells or arrays spots, and may also be used to quantify dot and slot blots. Array analysis and Toolbox modules also include multiplex analysis functionalities.

In addition to all the features included in CLIQS analysis package, CLIQS 1D Pro can: • Identify sample relationships across multiple gels using dendrograms and data matrices.

• Build up reference libraries of band patterns to share and classify new strains and samples CLIQS 1D Pro

• Provide optional CLIQS 1D 21CFR compliance for GLP/GMP laboratories Automated detection algorithms for fast and accurate image analysis

CLIQS 1D Pro is more advanced analysis software used primarily for band-pattern matching within individual DGGE, SSCP and RFLP gels that are important for cultivar experiments, evolutionary biology and population genetics. CLIQS 1D Pro has a powerful band matching feature, which is flexible and easy to use, while visual tools show the results of matching and identify similarities within an individual gel, including lane clustering via dendrograms.

CLIQS– Array Analysis

• Provide long-term archiving and storage of data from multiple gels • Offer comparison of each lane with any other lane within the database, with the results presented as dendrograms or tables that show all band and lane similarities, thereby enabling identification of lane relationships across many different experiments TotalLab 1D / CLIQS – Multiplex Analysis

ORDERING INFORMATION TotalLab1D CLIQS CLIQS1DPRO CLIQS1D21CFR

52

TotalLab 1D analysis module for agarose and PAGE gels; available only with the purchase of MicroDOC1D models Core Laboratory Image Quantification Software (1D Image Analysis of DNA & protein, Western blotting . Colony counting and basic 2D spot measurement Core Laboratory Image Quantification PRO Software (Accurate comparison of banding patterns in samples across multiple gels/experiments) Core Laboratory Image Quantification PRO Software (Accurate comparison of banding patterns in samples across multiple gels/experiments) 21CFR compliance for GLP/GMP laboratories Automated detection algorithms for fast and accurate image analysis

VISUALISATION & DOCUMENTATION

documentation • 12.1 MEGA PIXEL DIGITAL CAMERA* • IMAGE VISUALISED wITHIN A LARGE 8” TFT

Technical Specifications COLOUR

MONITOR

• LIGHT

MICRODOC

wEIGHT COMPACT HOOD wITH EASy ACCESS

DOOR AND BUILT-IN INNER LIGHTS

• INTEGRAL MICROSwITCH TURNS OFF UV TRANSILLUMINATOR AND TURNS ON INTERNAL LIGHT • CAN BE USED wITHOUT A COMPUTER • INCLUDES FLASH CARD, FLASH CARD READER AND ETHIDIUM BROMIDE FILTER

microDOC is a simple, inexpensive and ultra compact gel documentation system. It contains a digital camera with improved high-sensitivity CCD sensor and the latest image processor to guarantee superb resolution of 12.1 mega pixels*. For added convenience, limited space and budget requirements, microDOC can be used computer free. The image is viewed from a large 8" TFT colour liquid crystal display.

A variety of images can be captured in colour, clearly and easily, from agarose and other fluorescent gels, colorimetric gels, auto radiography film and blotting membranes. The system is fitted with a 55mm ethidium bromide filter and has a safety switch to turn off the UV Transilluminator when the door is opened. This also activates internal light for convenient gel manipulation. Files can be saved in a variety of formats (RAW, TIFF-RGB and JPEG) for later transfer to computer systems for storage and analysis with the highly recommended TotalLab™ 1D software.

shown with UV Transilluminator

The Mitsubishi P93 is a high speed, high resolution thermal printer that is easily lifted on and high resolution perfectly suited to printing off transilluminator 12.1Mb digital camera images directly from the microDOC. Connected to the microDOC by a BNC cable, the Mitsubishi P93 prints 325dpi images, up to 133 x 99mm in size.

separate power and light switches

CAMERA: EFFECTIVE PIXELS: CCD:

12.1 MILLION 1/1.7-INCH TYPE CMOS SENSOR WITH DIGIC 5 PROCESSOR 5 X ZOOM /4X DIGITAL ZOOM: MAX. APERTURE: F/2.8 (W) - F/4.5 (H) 15 - 1/4000S. (TOTAL RANGE) SHUTTER SPEED: RAW, TIFF-RGB, JPEG FILE FORMATS: STORAGE MEDIA: 8GB MEMORY CARD WITH BUILT-IN WI-FI COMPUTER INTERFACE: HI-SPEED USB (MINI-B COMPATIBLE) +3 CLOSE UP AND ETBR; OPTIONAL SYBR GREEN FILTER: VIDEO OUT: NTSC/PAL 8.0" TFT LIQUID CRYSTAL SCREEN: DISPLAY FORMAT: 800 X 600 PIXELS LUMINANCE: 350 CD/MM2 CONSTANT RATIO: 300 : 1 DISPLAY MODE: NTSC/PAL/SECAM MODE SWITCHABLE VIDEO AND AUDIO SWITCHING CHAMBER, MICRODOC: HOOD DIMENSION: 290 X 220 X 320MM (WXDXH) MULTI POWER FOR CAMERA, INNER LIGHT LAMP, SOURCE: TFT SCREEN INNER WHITE LAMP: 2 TUBES SAFETY DOOR SHUTS DOWN UV TRANSILLUMINATOR SWITCH: WHEN DOOR IS OPENED UV TRANSILLUMINATOR, MULTIDOC: 254, 312 OR 365NM (SPECIFY) WAVELENGTH: FILTER SIZE: 21 X 21CM LIGHT SOURCE: 6X 8W TUBES

internal illumination when door is open

microDOC BASIC

microDOC BASIC

Built-in Wi-Fi technology from the camera may be used within the microDOC camera to facilitate instantaneous wireless transfer of images to a computer or smart device - no cable connection required. Set-up requires a broadband internet connection and a DHCP-enabled wireless router. Minimum system requirement for software installation is Windows XP.

microDOC BASIC is a simple low-cost system comprising a lift-off dark room hood and 12.1 megapixel digital camera, through which the gel is viewed directly. This system can be supplied with optional TotalLab 1D Analysis Software and any one of the 21x21cm transilluminators

ORDERING INFORMATION Gel Documentation Systems Compact Gel documentation system microDOC System with UV Transilluminator (UVTS312) microDOC System with UV Transilluminator (UVTS254) microDOC System with UV Transilluminator (UVTS365) microDOC System with UV Transilluminator (UVTS254/312) microDOC System with UV Transilluminator (UVTS254/365) microDOC System with UV Transilluminator (UVTS312/365) microDOC Basic System with lift-off dark room hood and camera only

System only MDOC MDOCUV312 MDOCUV254 MDOCUV365 MDOCUV254/312 MDOCUV254/365 MDOCUV312/365 MDOCBASIC

Accessories PRNT MDOCEB MDOCSBRG

PRTPAP MDOCWLB MDOC-WIFI

Mitsubishi Thermal Printer for use with Microdoc - 110/240V Microdoc ethidium bromide filter Microdoc SYBR filter

For 110 V AC, please add ‘-$’ as a suffix to the appropriate product code

Including TotalLab 1D Analysis Software MICRODOC1D MDOCUV3121D MDOCUV2541D MDOCUV3651D MDOCUV254/3121D MDOCUV254/3651D MDOCUV312/3651D MDOCBASIC1D Replacement thermal printer paper White light box for Micro doc Optional 4GB wireless memory card * specification of camera is subject to change, usually by improvement

53

• PRE-FOCUSED 5

MEGA PIXEL

CAMERA wITH AUTO-EXPOSURE

FOR ALMOST INSTANTANEOUS

HIGH RESOLUTION GEL IMAGING;

CMOS

SENSOR FOR IMPROVED

LIGHT SENSITIVITy

• 6MM LENS, F1.2 APERTURE SIzE, wITH MANUAL ADjUSTMENT

• INTERCHANGEABLE FILTER SLIDE – 620NM ETHIDIUM

BROMIDE FILTER AS STANDARD;

520, 560

AND

580NM

FILTER

OPTIONS FOR RUNSAFE,

SyBR

STAIN AND OTHER

FLUORESCENCE APPLICATIONS

• INTERNAL

wHITE

LED –

HELPS

GEL POSITIONING AND FOCUSING

HIGH PERFORMANCE

gel documentation The omniDOC systems offer high performance gel documentation and analysis at affordable costs. By providing many of the features incorporated within the highest specification systems, but without the added price premium, each omniDOC system presents a simple but sophisticated imaging solution. A high resolution 5 mega pixel camera (CMOS sensor for improved light sensitivity) with slide-out UV transilluminator, and optional blue epi-illumination module and white light table, makes the omniDOC suitable for imaging most fluorescent and colorimetric gels, while a USB port requires a cable to connect the dark room assembly to an external PC for control. Imaging applications are made easy by a pre-focused camera that requires little or no manual adjustment, while simple one-click image acquisition and analysis software guides the user through every step of the gel documentation process. A front LED indicator panel reveals at a glance the light source in use, whereas a viewing screen with universal filter and spring-loaded cover facilitates safe and convenient gel inspection. All omniDOCs are constructed from corrosion resistant ABS and feature a viewing pane with universal amber filter for gel inspection, which may be covered by a spring-loaded panel during documentation.

OMNIDOC

• SLIDE-OUT 312NM

TRANSILLUMINATOR

• LARGE 21X26CM

FILTER AREA

Camera

NE W

• High resolution 5MP camera with CMOS sensor for enhanced light sensitivity • Lens: focal length 6mm; aperture size F1.2; autofocusing • Filter slide with four filter options to perform an extensive range of fluorescence applications Filter

• Filter slide with four interchangeable filter options: • 620nm filter (standard) – EtBr, Gel Red & SafeView Classic • 520nm filter (SYBR) – Gel Green, Midori Green, run- SAFE, SYBR Green I & II, SYBR Gold & SYBR Safe • 560nm filter (yellow) – as per 520nm filter but also including SYPRO Orange • 580nm filter (orange) – EtBr, Gel Green & Red, Safe- View Classic; SYBR Green I & II, SYBR Gold & Safe; SYPRO Orange & Ruby

Typical Applications Documentation and analysis of DNA, Safe Stained and Protein gels

54

DNA – use the slide-out UV transilluminator to capture images of DNA gels stained with EtBr and SYBR dyes

Blue light – LED epiillumination module allows visualisation of some stains with better clarity and without DNA damage – e.g. runSAFE

White light table – use plugin white light table to view coomassie blue and silver stain protein gels; may also be used to view tissue slides and autoradiographs

Autoradiographs – high resolution 5MP camera captures images in high detail, especially when scrutinising separation between closely located bands or spots

WIRELESS CONNECTIVITY

The omniDOC-i shares all of the same features of the standard omniDOC, but with the added benefit of wireless connectivity to a remote laptop or tablet. Simply install the omniDOC image acquisition and analysis software on a laptop or tablet, place the gel on the transilluminator or white light table within the darkroom, and then begin image capture, using your preferred excitation source and filter, either by ‘pressing’ the tabs on the omniDOC’s front panel colour touchscreen, or by following the prompts within the software on your laptop or tablet. Once image acquisition is complete the gel may be analysed immediately using the complimentary analysis software included, or saved for later to perform analysis at a more convenient time and place. The software is downloadable as an app to iPAD, and Windows and Samsung Android tablets to provide full touch-screen remote control, making the omniDOC-i probably the most portable and versatile imaging system on the market.

• wHITE

LIGHT TABLE AND BLUE

LIGHTS ALLOw EASy SwITCHING BETwEEN ETHIDIUM BROMIDE,

SAFE STAINED AND PROTEIN GELS

• wI-FI CONNECTION FOR wIRELESS REMOTE CONTROL AND IMAGE

TRANSFER TO COMPLIMENTARy

• USB PORT FOR PC CONNECTIVITy Technical Specifications • 3.5” 64K colour TFT display shows at a glance the excitation source in use, and provides full manual touchscreen control of the excitation source, UV intensity and exposure time.

omniDOC IMAGE CAPTURE AND ANALYSIS SOFTWARE – use the included software to...

Acquire, store and manipulate images

Analyse, document and quantify gels

Use intuitive touchscreen control for image acquisition in a few simple steps

Following image acquisition use the intuitive touchscreen control software for analysis

Adjust the exposure time, altering the UV intensity or manipulating the iris on the camera if required

Load the newly acquired image, or select one stored previously in TIFF, JPEG, BMP or GIF image format

Select your light source: UV, blue or white light

Select the gel or dot blot type from one of four options

Use Toolbox function to change default settings for excitation source & exposure time, or apply advanced features like saturation detection & date stamp

‘Tap and drag’ rectangular boxes on your tablet to define the sample lanes to be analysed

Image Freeze – minimise UV damage nucleic acid gels by ‘freezing’ the gel image and switching off the transilluminator ahead of image capture or printing

Set the level of sensitivity and define the base line for subtraction

OMNIDOC OMNIDOC-I UV TRANSILLUMINATOR 312NM, 21X26CM (WXL); 6X8W TUBES 5 MEGA PIXELS (2592X1944 PIXELS MAX) RESOLUTION SENSOR CMOS, 1/2.5” LENS 5MM FOCAL LENGTH; APERTURE F1.2 IMAGE BIT-DEPTH 12-BIT (0-4095 GREY LEVELS) SENSOR FILTER CAMERA 620NM ETBR (STANDARD); OPTIONAL 520, 560, 580NM FILTERS IMAGE STORAGE PC OR LAPTOP LAPTOP, PC OR IPAD, AND WINDOWS®,SAMSUNG ANDROID TABLETS CONNECTION TO USB TO PC WI-FI TO PC OR TABLET OPERATING DEVICE OPERATING SYSTEM WINDOWS® 7 (64BIT & 32BIT) / XP / VISTA REQUIREMENTS FOR SOFTWARE DARK ROOM 410 X 405 X 570MM (W X D X H) ASSEMBLY DIMENSIONS FRONT PANEL DISPLAY LED 3.5” 64K COLOUR TFT DISPLAY; TOUCHSCREEN VIEWING WINDOW 560NM UNIVERSAL ORANGE FILTER WHITE LIGHT 6X1W LED (STANDARD) FOR GEL POSITIONING WHITE LIGHT TABLE 21X26CM FILTER; CONNECTS INTERNALLY TO DARKROOM (OPTIONAL) BLUE LED EXCITATION WAVELENGTH 470NM; CONNECTS INTERNALLY TO DARK ROOM EPI-ILLUMINTION MODULE (OPTIONAL) SAFETY INTERLOCK SWITCH ON FRONT DOOR SAFETY PANEL; DISCONNECTS UV TRANSILLUMINATOR ON OPENING; COMPLIES WITH CE, FCC STANDARDS

Acquire and save image for...

Perform density analysis...

Analysis

And then molecular weight analysis; use software to save as an image file format of your choosing or export into Microsoft Excel as a CSV file for further data analysis

USB PORT WI-FI FORMAT POWER RATING WEIGHT

FOR PC

FOR UPDATES AND

CONNECTION

MAINTENANCE

WIRELESS N, WIRELESS G DUAL VOLTAGE: 110-230 VAC 25KG

ORDERING INFORMATION OMNIDOC omniDOC Gel Documentation System with 620nm (EtBr) emission filter & 312nm UV transilluminator* OMNIDOCSAFE omniDOC plus Blue LED Epi-illumination Module (OMNIDOC-BL), and 520, 560 & 580nm filters (OMNIDOC-SYBR, -AF560 & -AF580)* OMNIDOCPRO omniDOC plus White Light Table (HPDOC-WLT)* OMNIDOC-PROSAFE omniDOC plus Blue LED Epi-illumination Module (OMNIDOC-BL), and 520, 560 & 580nm filters (OMNIDOC-SYBR, -AF560 & -AF580); and White Light Table (OMNIDOC-WLT)* OMNIDOC-i omniDOC-i Gel Documentation System with 620nm (EtBr) emission filter & 312nm UV transilluminator† OMNIDOC-iSAFE HPDOC-i plus Blue LED Epi-illumination Module (OMNIDOC-BL), and 520, 560 & 580nm filters (OMNIDOC-SYBR, -AF560 & -AF580)† OMNIDOC-iPRO HPDOC-i plus White Light Table (OMNIDOC-WLT)† OMNIDOC-iPROSAFE omniDOC-i plus Blue LED Epi-illumination Module (OMNIDOC-BL), and 520, 560 & 580nm filters (OMNIDOC-SYBR, -AF560 & -AF580); and White Light Table (OMNIDOC-WLT)† OMNIDOC-AF580 Amber Filter, 580nm OMNIDOC-WLT Optional White Light Table OMNIDOC-BL Optional Blue Light modules OMNIDOC-AF560 Amber Filter, 560nm OMNIDOC-F1 Viewing window, Amber Filter, 560nm (Supplied as standard) OMNIDOC-SYBR Optical SYBER Green Filter OMNIDOC-EB Optical EtBr Filter (Supplied as standard) *Requires a PC or laptop with USB cable;

†

Requires a PC, laptop or tablet with Wi-Fi connection

55

rockers and shakers • ORBITAL,

RECIPROCAL

Four different models of rockers and shakers are available, with each offering benefits that include: outstanding uniform motion and low noise; microprocessor based keypads with digital control and display of preset time, continuous time, action scale and speed; and high quality stainless steel platforms with pop-on / pop-off installation holes to secure samples. All models are lightweight and portable for easy transportation from the bench to incubator and cold room alike, while additional platforms may be added for increased capacity.

&

ROCkING MODELS wITH

30X30CM SHAkING-PLATFORM & NON-SLIP RUBBER MAT • 2-D/3-D

SHAkER

ACCOMMODATES OPTIONAL HyBRIDISATION wATER BATH

• ADDITIONAL

PLATFORMS

CW-23

DOUBLE CAPACITy

• DIMPLED MAT SUPPORTS 1.5ML, 15ML AND 50ML

Technical Specifications Cat. No. Motion

MS-NOR MS-NRC Orbital, single direction Linear, Reciprocating or alternating, bi-directional clockwise & anticlockwise shaking Application aeration of samples, incubation of western 0.5 to 5ml in volume, within blots and initial multi-well plates, standard mixing of reagents dishes and petri dishes Stroke Length/Tilt Angle 20mm 19mm Orbits/Shaking Cycle 0.1-10.0 Speed 0-200 rpm 5-100 rpm Timer 1-9999 mins, 1-9999 mins, 1 min increment 1 min increment Controller Digital microprocessor Digital microprocessor Display 4 digital red LED 4 digital red LED Operating Temperature 4-40°C 4-40°C Carry Capacity 10 kg 15 kg Optional Stacking Platform Yes Yes Special Functions 2 way direction Platform Dimension 30 x 30cm 30 x 30cm Operating Power 110 / 220V 110 / 220V Weight 7 kg 7 kg

TUBES

• THE

PERFECT SPEED AND TILT

FOR BLOTTING AND GEL STAINING

• THREE-DIMENSIONAL • DESIGNED BOXES

MOTION

FOR USE wITH

BLOT

Technical Specifications SPEED

18RPM (115V) / 20 RPM (230V)

MOTION

3-D, NUTATING

TILT ANGLE

5°

PLATFORM SIZE (WXD)

20X16.5CM

AMBIENT OPERATING RANGE 4-65° DIMENSION (WXDXH)

20.3X17.8X10.5CM

WEIGHT

0.88KG

ELECTRICAL

115/230V 50/60HZ

MINIMIX 3D SHAKER

MS-NOR; MS-NRC; MS-NRK

MS-NRK Rocking

prevents gels and membranes ideal for gentle, from drying out during foam-free washing of staining, blocking and delicate cell lines antibody incubations within tissue culture 12° 8° 5-100 rpm 5-100 rpm 1-9999 mins, 1-9999 mins, 1 min increment 1 min increment Digital microprocessor Digital microprocessor 4 digital red LED 4 digital red LED 4-40°C 0-50°C 15 kg 15 kg Yes Yes Angle 0-25° 30 x 30cm 33 x 33cm 110 / 220V 110 / 220V 8 kg 10 kg

MiniMix combines the motions of orbital shaking and rocking to produce a gentle, but thorough, 3-D action that is perfect for antibody incubation of western blots and staining gels.

The pitch and speed of MiniMix are optimised to generate a tilt angle that is large enough to ensure uniform distribution of solutions, yet small enough to prevent the centre of the blot from drying out. This has the added benefit of allowing users to work with minimal volumes, thus conserving valuable probes and antibodies.

A non-slip rubber mat keeps the boxes in place on the agitating platform, which, along with the housing, is very easy to clean and decontaminate after use. The MiniMix’s compact and light weight design allows it to be moved around the lab where needed. It is also safe for use in an incubator or cold room for temperature controlled reactions.

Blot Boxes These gel staining / blotting boxes are available in 4 sizes and are the ideal accessory for incubating blots or staining gels.

ORDERING INFORMATION MS-NOR MS-NRC MS-NRK CW-23 MW-WB

Orbital Shaker with 30 x 30cm platform and non slip rubber mat Reciprocal Shaker with 30 x 30cm platform and flat non slip rubber mat Rocking Shaker with 30 x 30cm platform and flat non slip rubber mat Combination 3-D shaker with 3-D shaking and rocking for maximal fluid movement; includes 33 x 33cm platform and flat non slip rubber mat Hybridisation Water Bath (ambient to 95°C) for use with CW-23

3DSHAKER BB9X6 BB11X8

MiniMix 3D Shaker with 20x16.5cm tray and non-slip rubber mat Gel/Blot box, 9.1 x 6.6cm, 3-5ml capacity, 10/pack Gel/Blot box, 11.7 x 8.9cm, 6-10ml capacity, 10/pack

56

CW-23 2-D or 3-D Combi

BB12X12 BB20X16.5

Gel/Blot box, 12 x 12cm, 1/pack Gel/Blot box, 20 x 16.5cm, 1/pack

vortex mixer This variable speed Vortex Mixer combines fast, efficient mixing with minimal vibration. Unlike other vortex mixers using elliptical orbits, its true circular orbit facilitates uniform sample-vortexing even at low speed. The CombiCup™ head, which is supplied as standard, accepts many different tube sizes, while the unique overmoulding technology used in its manufacture provides a sturdy plastic core with non-slip TPE that fits optional heads for microplates, microtubes, PCR strip tubes, 15ml and 50ml tubes and blood vials.

• POwERFUL, RELIABLE MOTOR wITH OPTIMISED COUNTER BALANCE

• CIRCULAR

• COMBICUP • VERSATILE

HEAD ATTACHMENT

ACCESSORIES FOR MICROPLATES

An optimised counter balance system minimises vibration and movement of the unit during operation, whereas its lightweight construction and small footprint allows it to be readily transported and used in areas where space is restricted. The unit is also safe for use in temperature controlled environments, and may be used within a cold room.

S0200-22

HEAD ACCEPTS A

VARIETy OF TUBE SIzES

The unit may be used in ‘touch’ or continuous mode: ‘touch’ mode being activated by simply depressing the sample head and then stopped by releasing the pressure.

S0200-21

ORBIT FOR EFFECTIVE

VORTEXING AT ANy SPEED

S0200-23

AND DIFFERENT TUBE SIzES

Technical Specifications SPEED RANGE

115V 230V

0 - 3400 RPM 0 - 2850 RPM

OPERATING MODES

TOUCH OR CONTINUOUS

AMBIENT OPERATING RANGE 4 - 65°C DIMENSIONS (WXDXH)

S0200-24

S0200-25

S0200-26

S0200-27

heaters and stirrers

14CM X 16CM X 13CM

WEIGHT

2.2KG

ELECTRICAL

115V OR 230V, 50/60HZ

• LARGE wORk SURFACE, 19X19CM • SPACE SAVING DESIGN, 20X23CM FOOTPRINT • CHEMICAL

RESISTANT wHITE

CERAMIC TOP PLATE

• SAFETy LEDS

INDICATE wHEN

HEATING OR STIRRING

• qUICk

AND EASy ADjUSTMENT

OF HEATING/MIXING

These hotplates, stirrers and hotplate-stirrers feature an exceptionally durable, chemically resistant, white ceramic work surface. Their space-efficient design (20 x 23 cm footprint), makes them ideal for use on crowded bench tops and inside cabinets.

Advanced microprocessor controls with convenient turn knobs allow quick, precise adjustment and maintenance of speed and temperature. A safety indicator LED on each front panel indicates when the

heating and stirring functions have been activated.

With a square 19 x 19 cm work surface, all three models are compatible with a wide variety of popular sizes of borosilicate glass beakers, flasks, bottles and other vessels.

The hotplate/magnetic stirrer model is supplied complete with a support rod for mounting thermometers and temperature probes.

Technical Specifications SPEED RANGE:

60-1500 RPM (STIRRING UNITS ONLY)

TEMP. RANGE:

AMBIENT +5° TO 380°C

PLATFORM:

19 X 19CM

CONTROL:

QUICK ADJUSTMENT KNOBS

DIMENSIONS:

20(W) X 23(D) X 11.5(H) CM

ELECTRICAL DATA:

120V, 60 HZ / 230V, 50/60 HZ

(HEATING UNITS ONLY)

ORDERING INFORMATION ORDERING INFORMATION VORTEX Vortex Mixer with general purpose head Optional Head Attachments S0200-21 for 24 x 1.5/2.0ml tubes, 24 x 0.5ml tubes and 32 x 0.2ml tubes (or 4 tube strips) S0200-22 for 1 microplate or 64 x 0.2ml tubes or 8 x 0.2ml tube strips

S0200-23 S0200-24 SO200-25 S0200-26 S0200-27

for 8 x 15ml and 8 x 12/13mm Diam. tubes for 6 x 50ml tubes for 12 x 1.5/2.0ml tubes, held horizontally for 4 x 15ml tubes, held horizontally for 2 x 50ml tubes, held horizontally

HOTPLATE HOTSTIR

STIR

Magnetic Stirrer, 19 x 19 cm

Hotplate, 19 x 19 cm Hotplate Magnetic Stirrer 19 x 19 cm

57

• DIGITAL MICROPROCESSOR CONTROL • TOUCH

SCREEN

INTERFACE

& GRAPHICAL

• CHAMBER TEMPERATURE: AMBIENT TO 85°C • TEMPERATURE RESOLUTION ±0.1°C • CHAMBER TEMPERATURE ACCURACy AT 37°C ± 0.2°C

Technical Specifications DISPLAY

3.5” 64K COLOUR-TFT DISPLAY

CONTROLLER

32-BIT MICROPROCESSOR-CONTROL

CONTROL INTERFACE

TOUCH SCREEN & GRAPHICAL INTERFACE

TIMER / RESOLUTION

CONTINUOUS; PROGRAMMABLE 1-9999’ WITH ALARM / SECOND

HYBRIDISATION

shaking incubators With a compact, space-saving stackable design and temperature uniformity to within ±0.2°C, the new hybridPRO hybridisation shaking incubators can be used for numerous temperature-dependent laboratory applications. In addition to the standard entry level model, the hybridPRO range includes four incubator models, each supplied in one of four shaking-platform formats – vortex, orbital, reciprocal or rocking - and customisable for nucleic acid hybridisation techniques with three rotisserie options. A large 3.6” colourtouchscreen control panel simplifies manipulation of speed, temperature and time within an easy to programme 3-line display, while a 32-bit microprocessor provides the temperature uniformity and stability necessary to support the most temperature-sensitive applications. A modern design, stylish front door panel closes upon the stain-resistant interior of a robust metal chassis designed to withstand the stresses of frequent daily usage.

Standard NHYBRIDBASIC

NHYBRIDBASIC is a cost-effective system for homogeneous temperature control during routine incubations. Advanced ventilation design technology controlled by a digital microprocessor maintains temperature uniformity and stability to within ±0.2°C. A stain-resistant interior protects

against spillages, while two removable stainless steel meshshelves ensure that airflow remains unrestricted. Incubations may be set in precise 0.1°C increments under a continuous or programmable timer function.

TEMPERATURE CONTROL RANGE / RESOLUTION AMBIENT +5°C TO 85°C / 0.1°C TEMPERATURE UNIFORMITY/ ACCURACY AT 37°C ±0.2°C

Vortex NHYBRIDVX

TEMPERATURE CALIBRATION YES PLATFORM DIMENSIONS 27X20CM (20X30CM FOR NHYBRIDVX) DATA-LOGGING CAPACITY RS-232 OPERATING VOLTAGE

110/220V~ 50/60HZ (DUAL, SELECTABLE)

CHAMBER DIMENSIONS (W X D X H)

INNER EXTERIOR

WEIGHT

29KG

NHYBRIBVX has the same features as the NHYBRIDBASIC, but also includes a vortex platform for four microplates. The vortex platform is 20x30cm in size, and microprocessorcontrolled to vibrate at speeds ranging from 50-1500 rpm.

Three optional rotisseries may be purchased separately to allow the NHYBRIDVX to be adapted for hybridisation techniques performed in 40mm, 15ml and 50ml tubes.

34 X 22.5 X 26CM 44 X 46 X 45CM

Technical Specification for Shaking-platform models only Incubator Model Shaker motion Speed Optional rotisserie speed Resolution

ORDERING INFORMATION NHYBRIDBASIC HYB-SSMP NHYBRIDVX NHYBRIDROC HYB-SH HYB-8RT HYB-16RT HYB-24RT HYBBT40X150 HYBBT40X200 HYBBT40X300

58

Incubator only with 2 stainless steel shelves Stainless Steel Mesh Plate with 4 holders 32.5 x 34.5 cm Vortex Incubator with 200x300mm platform for 4 microplates Rocking Incubator with 270x200mm platform Supporting Holders for Stainless Steel Mesh Plate, pack of 4 35 mm tube Rotisserie for 8 tubes* 50 ml conical tube Rotisserie for 16 tubes* 15 ml conical tube Rotisserie for 24 tubes* 1x Glass tube 40x150mm (d x l) for HYB-8RT 1x Glass tube 40x200mm (d x l) for HYB-8RT 1x Glass tube 40x300mm (d x l) for HYB-8RT

NHYBRIDVX Vortex 50-1500rpm 5-100rpm 1rpm

HYBRIDORB HYBRIDREC HYB-8RT HYB-16RT HYB-24RT NHYB-P2720 NHYBFH-250 NHYBFH-500 NHYBFH-250-SET NHYBFH-500-SET HYBBT40X150 HYBBT40X200 HYBBT40X300 *tubes not included

NHYBRIDORB Orbital (clockwise & anticlockwise) 0-200rpm 5-100rpm 1rpm

NHYBRIDREC Reciprocal 5-100rpm 5-100rpm 1rpm

NHYBRIDROC Rocking 5-100rpm 5-100rpm 1rpm

Orbital Incubator with 270x200mm platform Reciprocal Incubator with 270x200mm platform 1x Rotisserie for 8x40mm glass tubes* 1x Rotisserie for 16x50ml disposable conical tubes* 1x Rotisserie for 24x15ml disposable conical tubes* Additional 27x20cm platform to double capacity of incubators 1x250ml flask holder for NHYBRIDORB & NHYBRIDREC platforms 1x500ml flask holder for NHYBRIDORB & NHYBRIDREC platforms 5x250ml flask holders for NHYBRIDORB & NHYBRIDREC platforms 4x500ml flask holders for NHYBRIDORB & NHYBRIDREC platforms 1x Glass tube 40x150mm (d x l) for HYB-8RT 1x Glass tube 40x200mm (d x l) for HYB-8RT 1x Glass tube 40x300mm (d x l) for HYB-8RT

Orbital NHYBRIDORB

NHYBRIDORB includes as standard a bi-directional 27x20cm orbital shaking platform that provides both clockwise and anticlockwise motion. Optional flask holders may be fitted to the orbital shaking platform to accommodate up to five 250ml or four 500ml flat-bottomed flasks, making the

NHYBRIDORB a suitable bench top shaker for growing overnight cultures of bacteria, yeast and insect cells. The shaking-platform may be replaced by one of three hybridisation rotisseries, which are available as options.

Standard drying agar plates & microbial

Reciprocal

plating techniques

NHYBRIDREC

NHYBRIDREC is a 27x20cm reciprocal shaking-platform incubator that may be adapted to accommodate 250ml and 500ml flat-bottomed flasks. The shaking-platform has a reciprocal motion that is perfect for incubation of western

Typical Applications

blots and microbial cultures, and may be replaced by one of three optional hybridisation rotisseries. An optional platform with four 10cm pillars mounts comfortably on the shaking platform to double capacity if required.

Vortex enzyme assays and nucleic acid hybridisation

Orbital overnight culture growth of

bacteria, yeast & insect cells

Rocking

Reciprocal staining, blocking & antibody

NHYBRIDROC

NHYBRIDROC includes a 27x20cm rocking platform to provide the perfect motion and tilt angle to prevent gels and membranes from drying out during staining, blocking and antibody incubations. Interchangeable hybridisation

rotisseries may be exchanged for the rocking platform to allow the NHYBRIDROC to be customised for the full range of nucleic acid hybridisation applications, including northern and Southern blotting techniques.

incubations

Rocking nucleic acid hybridisations: Northern & Southern blotting techniques

Hybridisation Rotisserie Options for all models except basic:

Extra 27x20cm Platform Option: For Orbital & Reciprocal models

Extra 27x20cm Platform Option: For Orbital & Reciprocal models

HYB-16RT and HYB-24RT for 16x50ml & 24x15ml conical tubes

HYB-8RT for 8x40mm

MOTORISED PIPETTOR

omnipet-m

• SUITABLE FOR 0.5ML 100ML PIPETTES • ERGONOMICALLy

SHAPED

HANDLE

omniPET-M is a motorised powered pipette filler with LCD display designed for cordless work with 0.5-100ml glass or plastic pipettes. Its lightweight handle, together with smooth pushbuttons and switches ensure effortless pipetting even during extensive use. The LCD display indicates setting options and level of battery charge.

• LCD

DISPLAy

• SENSITIVE

VALVES FOR PRECISE

wORk wITH LOw VOLUME

Different operational modes may be selected depending on pipetting volume and viscosity of liquid. Liquid aspiration speeds can be adjusted to HIGH or LOW while dispensing can be by gravity (GRAV) or supported by the pump (BLOW) which empties the pipette with blow out. To protect the unit against overfilling, omniPET-M is equipped with both PTFE filters and a safety valve. To protect samples from cross contamination, filters and pipette holders can be easily exchanged and autoclaved.

The powerful re-chareable Ni-MH battery allows many hours of continuous work with the LCD display indicating when unit should be recharged. The battery is protected against overcharging by timing and thermal systems. omniPET-M is supplied with a charging stand.

TO

PIPETTES LOw BATTERy LIGHT INDICATOR

• PROTECTED

By FILTER AND

SAFETy VALVE

• AUTOCLAVABLE

NOSEPIECE

AND PIPETTE HOLDER

CHARGING STAND

Technical Specifications

NEW

AUTOCLAVABILITY

NOSEPIECE, PIPETTE HOLDER, FILTER

FILTER

HYDROPHOBIC PTFE 0.2µM

PIPETTE TYPES

GLASS OR PLASTIC 0.5-100ML

ORDERING INFORMATION OPM

omniPET-M motorised powered pipette filler, with charging stand

59

• COMPLETELy

AUTOCLAVABLE

• CONTINUOUSLy • REDUCED

SINGLE CHANNEL

omnipette ADjUSTABLE

SPRING PRESSURES

MINIMISE FATIGUE

• COMFORTABLE,

ERGONOMIC

HAND GRIP

• UV RESISTANT • EIGHT

DIFFERENT MODELS

omniPETTE requires minimal maintenance. Its precise, selflocking stainless steel micrometer accurately adjusts the stroke of the polished, acid-resistant stainless steel piston. Each piston is machined to very fine tolerances which, in conjunction with its self-lubricating O-ring and PTFE seal, minimises the need for attention. Using a series of height adapters, the tip ejector position on the shaft is adjustable to allow the pipette’s use with virtually all brands of tips.

COVERING THE RANGE FROM

0.1µL

TO

Eight models of single channel omniPETTE ENLARGED cover all volumes from 0.1µl up to 10ml. A plunger button for continuously adjustable volumeter with digital user comfort readout allows simple and accurate dispensing. The robust construction along with the low thermal coefficient of the body of each pipette will prevent hand heat affecting sample EASY-TURN measurements and reproducibility, even in volume adjustment prolonged usage. Being lighter than many wheel other similar pipettes, they are more comfortable to hold and operate for extended periods - even in the smallest hand. This DIGITAL feature will also reduce the incidence of volume display operator fatigue and Repetitive Strain Injury.

10,000µL

EJECTOR BUTTON maximise individual comfort

LIGHT WEIGHT lighter than many similar models

COMPLETELY AUTOCLAVABLE SLIM HANDLE for optimum comfort

PLASTIC GRIP for easier tip ejector removal

AIRTIGHT SEAL

NEW

Each model is supplied with its own individual certificate of calibration, as a guarantee of the unit’s quality.

PTFE / O-ring

Piston/Seal assembly

ADJUSTABLE EJECTOR to allow a wider choice of tips

Free Calibration key supplied with each pipette

ORDERING INFORMATION Cat. No.

Volume Range

Accuracy %

Coeff. Variation %

Cat. No.

Volume Range

Accuracy %

Coeff. Variation %

CV2 CV10 CV20 CV100

0.1 to 2µl 0.5 to 10µl 2 to 20µl 10 to 100µl

±12 to 1.5 ±4.0 to 0.5 ±3.0 to 0.8 ±1.6 to 0.8

±6.0 to 0.7 ±2.8 to 0.4 ±1.5 to 0.3 ±0.8 to 0.2

CV200 CV1000 CV5000 CV10000

20 to 200µl 100 to 1000µl 1 to 5ml 1 to 10ml

±1.2 to 0.6 ±1.6 to 0.6 ±1.2 to 0.5 ±2.5 to 0.5

±0.6 to 0.2 ±0.4 to 0.15 ±0.5 to 0.15 ±0.6 to 0.2

Cat. No.

Description

Cat. No.

Description

CV-4POS

Pipette Stand, 4-position

CV-RS

Rotating Pipette Stand, 6-position

60

MULTI-CHANNEL

omniPETTE Multi-Channel Pipettes allow comfortable pipetting by both right and left handed users. The plunger and ejector buttons are ergonomically located and operate smoothly. For comfortable pipetting in any direction, the tip manifold rotates 360º. Each model features a revolutionary suspension system which allows the shafts to move independently and so retract slightly when they are pressed against a row of pipette tips.This ensures that all tips secured on their individual shaft with the minimum of effort - and never fall off! In addition, an innovative ejector bar is curved, allowing the tips to be pushed off in steps, therefore reducing the amount of force required for ejection.

The use of advanced, lightweight materials (in both the body of the EASY-TURN pipette and the piston assemblies) volume adjustment wheel results in a pipette that will stand up to

NEW

• COMPLETELy ENLARGED plunger button for user comfort

Each channel of the pipette has an individual, precision piston assembly to ensure accuracy and reproducibility from one pipetting series to the next, as well as between channels. The micrometer is continuously adjustable for selection of whole or fractional volumes.

MANIFOLD

• INDIVIDUAL • CURVED,

LIGHT WEIGHT DIGITAL

• LIGHTwEIGHT BODy • UV RESISTANT

ergonomic design for comfort

PRECISION PISTONS

LOw FORCE EjECTOR

BAR

lighter than many similar models

volume display

SPRING PRESSURES

MINIMISE FATIGUE

• ROTATING

EJECTOR BUTTON

constant use, yet is easy to hold, even during extended pipetting sessions.

• REDUCED

AUTOCLAVABLE

• EIGHT

DIFFERENT MODELS

COVERING THE RANGE FROM

0.1µL

SLIM HANDLE

TO

300µL

for optimum comfort

omniPETTE Multi-Channel Pipettes are available in 8 and 12 channel models with volume ranges designed to meet liquid handling requirements from 1.0 to 300µl.

ROTATING MANIFOLD pipette and eject in any direction

The performance of every pipette is checked by gravimetric method and the results of test are printed in pipette Quality Control Certificate.

a revolutionary suspension system ensures that all tips are secured on their individual shaft

INDIVIDUAL PRECISION PISTONS

CURVED EJECTOR BAR

ensure accuracy and reproducibility between channels and pipetting series

reduces the force required for tip ejection

ORDERING INFORMATION Cat. No.

Channels

Volume Range

Accuracy %

Coeff. Variation %

Cat. No.

Channels

Volume Range

Accuracy %

CV8-10

8

1 to 10µl

±8.0 to 1.2

<8.0 to 1.2

CV12-10

12

1 to 10µl

±8.0 to 1.2

<8.0 to 1.2

CV8-50

8

5 to 50µl

±4.0 to 1.6

<2.5 to 0.6

CV12-50

12

5 to 50µl

±4.0 to 1.6

<2.5 to 0.6

CV8-200

8

20 to 200µl

±3.0 to 1.0

<1.5 to 0.6

CV12-200

12

20 to 200µl

±3.0 to 1.0

<1.5 to 0.6

CV8-300

8

50 to 300µl

±1.6 to 1.0

<1.5 to 0.6

CV12-300

12

50 to 300µl

±1.6 to 1.0

<1.5 to 0.6

Cat. No.

Description

CV-MS

Pipette Stand, 3-position

Coeff. Variation %

61

• UNIqUE MULTI-FLOw

AIR

COOLING TECHNOLOGy

• INCLUDES

EASy ACCESS

ROTOR wITH OPTIONAL

STRIPSPIN

• RAPID

ACCELERATION AND

DECELERATION TIME

• EXCEPTIONALLy

qUIET wITH

EASy TO USE CONTROL PANEL

• COLD

ROOM COMPATIBLE

• ADAPTERS FOR 0.2ML 0.5ML TUBES • TwO

AND

yEAR wARRANTy

PRISM™

microcentrifuges The Prism™ Microcentrifuge is an easy-to-use, high performance microcentrifuge in a modern and attractive housing. The new friction fit lid latching system combines reliability and safety with easy operation, while the updated control panel with a single large control knob and easy to read Liquid Crystal Display provides an intuitive interface for operation. Speed (in RPM or G-Force) and run time can be quickly and accurately set, with actual speed and time remaining displayed during a run.

This latest air-cooled microcentrifuge combines high speed, quiet operation, and effective air cooling in one compact instrument. Prism’s uniquely designed 24-place rotor allows easy access to the tops of sample tubes, allowing for faster work flow. The individual tube cavities in the solid rotor, support tubes along their length to eliminate tube deformation or cracking even at the highest speed settings. In the event of a tube failure, tube pieces and samples are confined to the sample well. The rotor is designed to hold all

Technical Specifications SPEED:

500 TO 15,000 RPM

MAX. RCF:

21,200 XG

SPEED INCREMENTS:

100 RPM

TIMER:

0.5-99 MINUTES OR CONTINUOUS

DIMENSIONS:

24 X 35 X 19CM

24 tube rotor

• EASy

ACCESS,

ML ROTOR

• SET

24

X

1.5/2.0

AND VIEw SPEED IN RPM

OR G-FORCE

• DIGITAL

SET AND DISPLAy OF

PARAMETERS

• qUIET

AND COOL RUNNING

• INNOVATIVE DESIGN

AND

BRUSHLESS

Technical Specifications SPEED:

500 TO 13,200 RPM

MAX. RCF:

16,300 XG

AMBIENT OPERATING RANGE 4° TO 35°C TIMER:

0.5-30 MINUTES OR CONTINUOUS, “QUICK” BUTTON FOR MOMENTARY OPERATION

DIMENSIONS:

23.5 X 29.3 X 21.6CM

2 year warranty

standard 1.5/2.0ml tubes (both conical bottom and skirted), and the snap on lid offers space for micro-filter filters that fit into the tops of the tubes. Adapters are available for processing smaller tubes, and the StripSpin™ Adapter fits onto the top of the rotor for centrifuging standard 8-tube PCR strips. The Prism™ Microcentrifuge offers a maximum speed of 15,000 rpm / 21,200 x g for very efficient separation of nucleic acids and protein samples. Rapid acceleration and deceleration reduce processing time, and the unique multi-flow air cooling system keeps the rotor and samples close to ambient temperature with minimal noise.

Spectrafuge Spectrafuge 24D is an economical high performance microcentrifuge, loaded with features. Its digital microprocessor allows precise control over a powerful brushless motor which can rapidly accelerate to a maximum speed of 13,300rpm/16,000 x g. The innovative, easy-access 24 x 1.5 ml rotor places the tubes on a plateau, where the user can easily grip their tops. Controls are simple, consisting of only two knobs.

Speed may be set in either rpm or g-force (rcf). The timer knob starts and stops the unit’s operation as well as setting run time parameters the last run settings remain in memory allowing repetitive centrifuging without resetting of parameters. Spectrafuge 24D is not only extremely quiet but is also cool running. With its efficient ventilation system, the unit remains cool during repetitive and prolonged operation.

Incorporating a self-cooling ventilation system, even during extended runs at maximum speed, sample temperature will remain close to ambient. Spectrafuge 24D may be used in a cold room.

ORDERING INFORMATION MC24 SPMC24 MC24-1205 C2400-SS

62

24-place Air Cooled Microcentrifuge with 24 x 1.5/2.0ml rotor, 230V Spectrafuge 24D complete with 24 x 1.5/2.0ml rotor, 230V Adapters for 0.5ml tubes, pk/6 StripSpin™ adapter for 0.2 ml tubes and strips

NEW

C-1222

Adapters for 0.2ml PCR tubes, pk/6

BENCH-TOP

refrigerated This new Prism™ Refrigerated Microcentrifuge sets a new standard in laboratory bench top centrifugation. Its 24-place rotor and high speed capability make it the ideal centrifugation solution for all molecular biology applications that use 1.5ml tubes and 0.2ml tubes and strips. An intuititous LCD control panel provides full user-control over speed, time and temperature, as well as a wipeclean surface for easy decontamination. Speed adjustments in increments as low as 100 rpm or 100 x g ensure precise control to a maximum 13,500 rpm, or 17,135 x g rcf.

Centrifugation may be either timed between 30 seconds and 99 minutes or performed continuously, while a ‘quick’ key is provided for short spins or cell pelleting.

Prism’s powerful refrigeration system maintains sample temperatures as low as -10°C and takes less than 8 minutes to reach 4°C from room temperature and which can be maintained even at maximum speed. A brushless motor drive allows the rotor to achieve its set speed quickly and effortlessly, while a computer-controlled isolation system ensures that the centrifuge operates free of vibration with a slight load imbalance; although shut

• MAXIMUM SPEED OF 17,135 ×G (13,500 RPM) ACHIEVED IN <16 SECONDS • RAPID-COOL FUNCTION ALLOwS

ROTOR TEMPERATURE TO REACH

4°C wITHIN 8 MINUTES • EXCEPTIONALLy

56DBA

qUIET

BRUSHLESS MOTOR,

• USER-FRIENDLy LCD

DISPLAy

FOR INPUT OF TEMPERATURE AND SPEED

• IMBALANCE

-

IN RPM OR RCF

DETECTION wITH

AUTOMATIC SHUT-OFF

• qUICk

SPIN FUNCTION IDEAL

FOR CELL ‘PELLETING’

NEW down occurs automatically, within 18 seconds and without sample disruption, if a significant imbalance is detected.

The unique design of the 24-place rotor allows easy access to all sample tubes; and individual tube slots within the solid aluminium rotor support each tube along its length and retain sample in the event of tube failure.

The rotor accepts 1.5 and 2.0ml tubes directly and smaller 0.5ml tubes using optional adapters. The optional StripSpin™ adaptor is connected to the top of the rotor to spin 0.2ml tubes and PCR strips. The unit’s rotor sits on a tapered shaft so that it may be removed easily to clean and autoclave.

• TwO

yEAR wARRANTy

Technical Specifications MAXIMUM SPEED

13,500RPM

MAXIMUM RCF

17,135 X G

TEMPERATURE RANGE

-10 TO +40°C

TIMER

0.5-99MINS; CONTINUOUS

DIMENSIONS (WXDXH)

27.7 X 43 X 24.8CM

WEIGHT

44LB / 20 KG

ELECTRICAL

120V~ OR 230V~ 50/60HZ; 500W

Solid aluminium rotor with sample capacity for 24 × 1.5/2.0 ml tubes, 2 × PCR strips or 16 x 0.2ml tubes

StripSpin adaptor for 0.2ml tubes

Simple paramter entry

ORDERING INFORMATION RMC24 MC24-ROTOR

Refrigerated 24-place Microcentrifuge, 230VAC Rotor, 24x1.5/2ml tubes (included with RCM24)

MC24-1205 C2400-SS

Adaptors for individual 0.5/0.6ml tubes, pk/6 StripSpin™ adapter for 0.2 ml tubes and strips

63

• COMBI-ROTOR

FOR TUBES

AND STRIPS

• DOUBLE

THE CAPACITy OF

TRADITIONAL MINI CENTRIFUGES

• NEARLy

SILENT OPERATION

• STARTS & STOPS wITH OPENING/CLOSING OF THE

MULTIFUGE

microcentrifuges Unlike traditional mini centrifuges, the new multiFuge 12 eliminates the need to change rotors when switching between microtubes and PCR strips. The included, unique COMBIRotor is all that is required for running 12 microtubes and 4 PCR strips simultaneously.

With a fixed speed that produces 2,000 x g, this centrifuge is perfect for quick spin downs. Simply close the lid and the unit quickly ramps up to 5500 rpm. Open the lid, and the rotor quickly decelerates for removal of samples.

LID

At just 14cm wide and less than 11cm high, the multiFuge 12 truly is a personal centrifuge with unmatched capacity and flexibility.

NEW

Technical Specifications SPEED:

5,500 RPM / 2,000 X G

CAPACITY:

12 X 1.5 / 2.0 ML TUBES, 32 X 0.2 ML PCR TUBES, 4 X PCR STRIPS (8X0.2 ML)

DIMENSIONS:

14 X 20 X 11.2 CM

WEIGHT:

5 KG

Unique COMBI-Rotor

QuickSpin • SUPPLIED

wITH BOTH

STANDARD MICROTUBE AND

STRIP TUBE ROTORS

• IDEAL

FOR qUICk SPIN-DOwNS

AND MICROFILTRATION

• STARTS

AND STOPS IN SECONDS

• COMPACT

DESIGN

Technical Specifications MAXIMUM SPEED

6000RPM

MAXIMUM G FORCE

2,000 X G

CAPACITY

6 X 1.5/2.0 ML 2 X 0.2ML STRIPS

DIMENSIONS (W X D X H)

15 X 15 X 11.7 CM

WEIGHT

0.45 KG

Quickspin is perfect for microfiltration and rapid spin-down of sample from the walls and caps of microcentrifuge tubes.

Occupying less than 6 inches square of bench space, the Quickspin has a very small footprint, making it easy to use in the lab. Rotors and adaptors, which may be easily interchanged, are supplied as standard to accommodate 1.5 ml, 0.5 ml and 0.4 ml tubes, as well as 0.2 ml strips and individual tubes.

A highly durable stainless steel hinge pin facilitates easy opening of the translucent lid, while an on/off switch is located on the side of the centrifuge to start and stop operation. Alternatively, with the switch in the ‘on’ position, the centrifuge can be started and stopped by closing and opening the lid.

ORDERING INFORMATION MFUGE

MultiFUGE™ Mini Centrifuge, with COMBI-Rotor for 12 microtubes and PCR strips/tubes

QSPIN

QuickSpin complete with 1.5/2.0 ml rotor, strip tube rotor, 0.5 and 0.4 ml adapters, Purple lid

64

C0812-A5 C0812-A2

Adapters, 0.5 ml, pack of 6 Adapters, 0.2 ml, pack of 6

Compact, low profile design

MULTIGENE™ OPTIMAX

thermal cycler The new MultiGene™ OptiMax brings new speed and advanced features, providing premium performance at an affordable price. This simple to program unit is compact in design and is built to perform.

Programming is intuitive using the large display and multiple pre-programmed templates supplied. The OptiMax comes with standard built in 2-step, temperature optimization, touchdown and time increment protocols that are easy to adjust to meet cycling requirements. Lid temperature can be either turned down or off depending on user needs. The faster ramp rates allow more work to be done in a work day. OptiMax employs a new protocol optimisation process improving on older gradient features.

The OptiMax utilises FlexTemp™ technology that effectively separates the 96 well plate into 6 – (4 x 4) temperature regions that allow to the temperatures to be selected to test against. These regions are easily identified by the blue and black squares visible on the microplate platen. Instead of having the thermal cycler choose temperatures, the user can choose any temperatures they want within a 24°C range.

• UNIqUE FLEXIBLE PROGRAMMING ™ wITH FLEXTEMP TECHNOLOGy • FAST RAMP RATES 5°C HEATING/ 3.5°C COOLING • IMPROVED • SIMPLE • NO

Gene A

NEW

Gene B

T3 Gene C T3: 65°C

Gene C

Intuitive User Interface MultiGene™ OptiMax utilises an intuitive user interface. This user interface is friendly to the laboratory environment and consistent with what users of MultiGene™ enjoyed in earlier models. It can be used with lab gloves even if wet. The ramping speed and eliminated overshooting and undershooting which contributes to longer run times, have been improved.

CONDENSATION AFTER

Figure 1. Primers designed to anneal to various genes (e.g. Gene A, B, and C) typically have different annealing temperatures (T1,T2 and T3). To simplify workflow and increase throughput the Multigene Optimax can perform up to six different reactions, significantly reducing steps and time.

4°C

• 3 yEAR wARRANTy Technical Specifications SAMPLE CAPACITY

PROGRAMMABLE TEMPERATURE RANGE TEMPERATURE CONTROL TEMPERATURE ACCURACY/UNIFORMITY HEATING/COOLING METHOD GRADIENT TEMPERATURE RANGE

1 X 96 WELL PLATE, 12X 8X0.2ML STRIPTUBES, 96 X 0.2ML TUBES 4°C TO 99.9°C CALCULATED OR BLOCK ±0.5°C/±0.5°C PELTIER 30°C TO 99°C. TEMPERATURE OF 6-SEGMENT BLOCKS CAN BE SET INDEPENDENTLY

• Improved Throughput by reducing time • Simplified Workflow by reducing steps

vs.

Gene A Gene B T1: 51.7°C T2: 61°C

Simplified Workflow – Improved Throughput Multigene OptiMax may be programmed to operate between one and six different annealing temperatures of user choice, across the block.

T2

USER INTERFACE

OVERNIGHT COOLING AT

MultiGene™ OptiMax has fast heating rates of 5°C /sec and cooling at 3.5°C/sec. Overshooting and undershooting have been minimised so that reactions only see the temperatures they are supposed to see. (See figure) This also leads to faster runs time.

T1

wORkFLOw OVER

TRADITIONAL GRADIENT CyCLERS

MAXIMUM TEMPERATURE DIFFERENCE 24°C BETWEEN 6-SEGMENT TEMP. BLOCKS 6-SEGMENT TEMPERATURE BLOCKS 6 TEMPERATURE BLOCKS IN 4 X 4 WELL FORMAT PROGRAMMABLE LID TEMPERATURE 60° TO 65°C, 100° TO 115°C PROGRAM MEMORY 200 COMPLETE PROGRAMS

TEMP. INCREMENTS/DECREMENTS TIME INCREMENTS/DECREMENTS USER PROGRAM FOLDERS PASSWORD PROTECTED PROGRAMS COMMUNICATION

YES YES 50 SETS YES USB AND RS232

DIMENSIONS (W X D X H), WEIGHT ELECTRICAL

24 X 42 X 25 CM, 9 KG 240V OR 120V, 50/60HZ

PORTS

Fully Adjustable Heated Lid OptiMax’s heated lid is fully adjustable to provide optimum pressure for use with a broad range of tubes and microplates.

For optimum performance, the lid can also be programmed to hold different temperatures between 60° to 65°C and 100° to 115°C. The OptiMax lid prevents condensation and slides away and back from the user to provide full access to samples and reduce the risk of coming in contact with a hot surface.

NEW PC Viewer Option By downloading the software and attaching a USB cable to a Windows PC, the real time temperature profiles can be viewed as they are occurring, to allow monitoring of your system.

ORDERING INFORMATION BCMGO

MultiGene Optimax Thermal Cycler with 96 well block, 230V, for 96 x 0.2ml tubes or 1 x 96 well plate (other block formats available)

65

• MICROPROCESSOR

CONTROL

wITH DIGITAL PERFORMANCE

FOR PRECISE, ACCURATE CONTROL

• wIDE

TEMPERATURE CONTROL

RANGE wITH EXCELLENT UNIFORMITy

• RAPID

TEMPERATURE INCREASE

RATE

• LCD

SCREEN SHOwING TIMER

AND TEMPERATURE SIMULTANEOUSLy

• USER

TEMPERATURE

CALIBRATION

DIGITAL DRY BATHS

heating CUBE digital dry baths are available in single and dual block models, and comprise a comprehensive range of interchangeable blocks. Each digital dry bath is compact and easy-to-use Interchangeable quickchange blocks have rapid heat-up times and reproducible temperature uniformity and accuracy, and may be used in a variety of applications, which include: restriction digestion, BUN, melting agar, coagulation studies, hybridisation, Hot Start PCR® reactions and DNA denaturation. Because of an impermeable moulded aluminium alloy block construction, each Cube dry bath may also be adapted as a mini water bath incubator if desired.

Both dry baths incorporate a digital microprocessor controller for accurate temperature control in 0.1°C increments from ambient +5°C to 150°C. Rapid and easy programming is facilitated by the easy to use arrow keys on the sloped front panel, while both the temperature and running time are shown simultaneously on the conspicuous dual digital LCD display. Software options are available for data logging to a PC and computer control (please enquire).

Technical Specifications CAT. NO. TCDB-01 TCDB-02 1 2 NUMBER OF BLOCKS LCD DISPLAY DISPLAY HEATING POWER 125W 200W DIMENSIONS 15X23X13.5 MM 15X15X13.5 MM (W X L X H) DIGITAL MICROPROCESSOR CONTROLLER HEATING CHAMBER MOULDED ALUMINIUM ALLOY CHAMBER TEMPERATURE RANGE 5°C ABOVE AMBIENT TO 150°C 0.1°C TEMPERATURE INCREMENT WITHIN 0.2°C TEMP. UNIFORMITY AT 37°C WITHIN 0.2°C TEMP. ACCURACY AT 37°C YES TEMP. CALIBRATION TIMER UP TO 99(HR) : 59(MIN), CONTINUOUS SAFETY LEAK PROOF HEATING CHAMBER OVER TEMPERATURE PROTECTION SSR FAILURE DETECTION OPERATING TEMP. AMBIENT TO 40°C

MD-B0.5: 0.2ml tubes

MD-B0.5: 0.5ml tubes

MD-B0.5+1.5: 0.5/1.5ml tubes

MD-B0.5/1.5: 0.5/1.5ml tubes

MD-B1.5: 1.5ml tubes

MD-B13: 13mm Ø tubes

MD-B17: 1 7mm Ø tubes

MD-B20: 20mm Ø tubes

MD-B25: 25mm Ø tubes

MD-B29: 50ml cent. tube

MD-MP01-S: Microplate

MD-MP02-S: Micro / PCR plate

MD-MP01-D: Micro / Titerplate

MD-MP02-D: Micro / PCR plate

MS-BL95: Block Lifter

ORDERING INFORMATION

SINGLE BLOCK TCDB-01 The Cube Dry Bath Incubator (one block unit); without block 220V

Accessories MD-MP01-S MD-MP02-S MD-MP01-D MD-MP02-D MD-B0.5/1.5 MD-B0.5+1.5 MD-B0.2

66

Block for Microplate; Titerplate Plain Block for Single Block Unit Only Block for 96 wells deep Microplate or PCR plate for Single Block Unit Only Block for Microplate; Titerplate Plain Block for Dual Block Unit Only Block for 96 wells deep Microplate or PCR plate for Dual Block Unit Only Double side block: one side for 1.5 or 2.0 ml tube, 20 wells; Opposite side for 0.5 ml tube, 30 wells Combination block : for 0.5 ml tube, 12 wells and for 1.5 or 2.0 ml tube, 12 wells (on the same side) Block for 0.2 ml tube, 64 wells or for 0.2 ml PCR strip tubes for 8 wells x 8

DUAL BLOCK

TCDB-02

The Cube Dry Bath Incubator (dual block unit); without block 220V

MD-B0.5 MD-B1.5 MD-B13 MD-B17 MD-B20 MD-B25 MD-B29 MD-RS232 MD-DSLW

Block for 0.5 ml tube, 20 wells Block for 1.5 ml tube, 20 wells Block well size 13 mm, 20 wells Block for 15 ml centrifuge tube, 12 wells Block well size 20 mm, 12 wells Block well size 25 mm, 6 wells Block for 50 ml centrifuge tube, 4 wells RS232 Cable Single License Software Package for Datalogging

DIGITAL DRy BATH MICROBLOCk

Benchtop control of sample temperature has never been this easy or economical. The new microBlock fits almost anywhere and can even be used "on-the-go" in cars, boats or wherever a 12 volt power source is available. Its simple touch pad control with digital display is designed for "set and walk away" temperature selection and unrivaled accuracy. At less than 10.5cm. wide, microBlock is truly the first personal block incubator.

• COMPACT,

FITS IN THE PALM OF

yOUR HAND

• EXCHANGEABLE BLOCkS, 0.2 TO 50 ML TUBES • DIGITAL • CLEAR

FOR

TEMPERATURE CONTROL

COVER IMPROVES

TEMPERATURE UNIFORMITy

• PORTABLE (wITH CAR ADAPTER)

OPTIONAL

12V

Technical Specifications MD-MINI-B01

MD-MINI-B02

MD-MINI-B03

MD-MINI-B04

MD-MINI-B05

stirring

water bath A powerful magnetic stirring mechanism combined with high wattage heating allows each stirring water bath to maintain temperatures to a maximum 99°C. Available in 10 and 20 litre bath capacities, these water baths comprise as many as 3 stirrers for a maximum stirring speed of 1800rpm. Each bath includes a highly visible front-panel LCD, reproducible microprocessor control of temperature within 0.1°C increments, a corrosion resistant stainless steel interior and automatic alarm and safety shutdown mechanism. Optional data logging and PC control software is also available.

MD-MINI-B07

TEMPERATURE RANGE:

AMBIENT +5 TO 100˚C

TEMPERATURE ACCURACY:

± 0.5˚C

TEMPERATURE INCREMENTS:

0.1˚C

TEMPERATURE UNIFORMITY:

± 0.2˚C

DIMENSIONS:

11 X 15 X 10 CM

WEIGHT

500 G

• POwERFUL

MAGNETIC STIRRING

MECHANISM

• STIRRING SPEED UP TO 1800RPM • AVAILABLE

IN

10

• REPRODUCIBLE

AND

20

LITRE

TEMPERATURE

CONTROL wITHIN

0.1°C

Technical Specifications SWB-

10L-1

STIRRERS CAPACITY (

APPROX.)

INTERNAL DIMENSIONS

10L-2

20L-1

20L-3

1

2

1

3

10 L

10 L

20 L

20 L

24X30X15CM

30X50X15CM

TEMPERATURE

5°C ABOVE AMBIENT TO 99°C

HEATING POWER

600 W

STIRRING SPEED TIMER TEMPERATURE SAFETY

800 W

400 - 1500 RPM UP TO

99 HR 59MIN, CONTINUOUS 0.1°C

WARNING INDICATOR ON SCREEN, WITH ALARM AND AUTOMATIC SHUT DOWN

ORDERING INFORMATION MBDB-01 MD-MINI-B01 MD-MINI-B02 MD-MINI-B03

microBlock Digital Dry Bath with block lifter (Blocks sold separately) Block, for 0.2ml tubes (PCR Strip Tube), 32 wells, 6.35mm, depth 19mm Block, for 1.5ml tubes, 12 wells, 10.8mm, depth 28.5mm Block, for 15ml tubes, 6 wells, 17.3mm, depth 70mm

MD-MINI-B04 MD-MINI-B05 MD-MINI-B06 MD-MINI-B07

Block, for 50ml tubes, 2 wells, 29.2mm, depth 72mm Block, for 0.5ml tubes, 12 wells, 8.0mm, depth 25mm Block, for 2.0ml or 1.5ml tubes, 12 wells, 11.0mm, depth 30mm Block, for 1.5ml tubes, 12 wells, 10.9mm, depth 30mm

SWB-10L-1 SWB-10L-2 SWB-LID10

Stirring Water Bath 10L with 1 built-in stirrer, includes lid Stirring Water Bath 10L with 2 built-in stirrers, includes lid Transparent lid for 10L stirring water bath

SWB-20L-1 SWB-20L-3 SWB-LID20

Stirring Water Bath 20L with 1 built-in stirrer, includes lid Stirring Water Bath 20L with 3 built-in stirrers, includes lid Transparent lid for 20L stirring water bath

67

• IDEAL

FOR LOw TEMPERATURE

STORAGE

• SUITABLE FOR 0.5ML AND 1.5ML MICROTUBES + 2.0ML CRyOTUBES

• IDEAL

LOw COST

TRANSPORTATION

• COMPACT DESIGN – 185 X 110

X

60MM Lid

EPRAk STORAGE

microtube The Eprak is a low cost multi-purpose box manufactured in expanded polystyrene.

With its removable Insert Tray, it is ideal for use as both a work station and storage box for microcentrifuge tubes, of both 1.5ml (50) and 0.5ml (72) capacities as well as 2.0ml cryotubes (36).

Eprak can be used along with either liquid nitrogen or dry ice for flash freezing of samples, or the expanded polystyrene Insert Tray can be floated in a thermostatically controlled bath to allow defrosting or incubation of tube contents.

Insert Tray Base

• Expanded polystyrene allows controlled temperature changes

• Removable Insert Tray floats in water bath

• Can be used with crushed or dry ice, water and liquid nitrogen

• Defrosts or incubates tube contents • Inserts can easily be trimmed to a smaller size

coolcube • MAINTAINS 0°C

FOR

4

HOURS

• HOLDS BOTH MICROTUBES AND A PCR PLATE • CAN

BE USED AS A

wORkSTATION

PCR

Technical Specifications TEMPERATURE:

0°C FOR UP TO 4 HOURS

CAPACITY:

36 X 1.5ML 96 X 0.2ML 12 X 0.2ML PCR STRIPS 1 X 96 WELL PCR PLATE

DIMENSIONS:

CoolCube is designed to store samples safely on the lab bench without sample degradation caused by temperature increase or fluctuation. CoolCube maintains temperature at approximately 0°C for up to four hours. Simply place it in a freezer overnight and it will be ready to use.

One side of the CoolCube can be used as a convenient PCR workstation for 12 standard 1.5/2.0ml microtubes with one 96 well PCR plate, or with 12x 0.2ml strips or 96x0.2ml tubes. The other side may be used to hold up to 36 standard 1.5/2.0ml microtubes.

0°C CoolCube Sample Temperature vs. Time 5

T E M 0 P E R A T U -5 R E (°C)

-10 0

1

2

3

900G DURASTAR POLYMER

baths

• DURABLE • EASILy

ICECOOL

WEIGHT: MATERIAL:

5

PLASTIC

CLEANED

• EXCELLENT

LONG TERM THERMAL

STORAGE

IceCool Baths are constructed of durable plastic and offer both robustness and long life. They will also withstand reasonable use with dry ice. Lids are available for the large bucket.

ORDERING INFORMATION BC-5003 BC-5004 BC-5008

EPRAK with 1.5ml Microtube Inserts, pk/30 EPRAK with 0.5ml Microtube Inserts, pk/30 EPRAK with 2.0ml Cryotube Inserts, pk/30

CSLIB

Large IceCool Bath - White

68

4

Time (hours)

142 X 142 X 63.5MM

BC-5005 BC-5006 CLCB CSLIBLID

Replacement 1.5ml Insert Trays, pk/30 Replacement 0.5ml Insert Trays, pk/30 Coolcube Storage Rack

Lid for Large IceCool Bath - White

CSMIB

Mini IceCool Bath - White

6

TOUGH-TAGS

™

labelling systems H ot

Cold

Wet

DRy

™

Centrifuge and Vortex Compatible - Microcentrifuge tubes with wrap-around labels slide easily in and out of rotors without binding. they are ideal for use with all brands of centrifugal filters and concentrators. Tough-Tags are scuff and smear resistant, and cannot be removed by even the most vigorous shaking or vortexing.

Durable - Unlike paper and many other label materials, this advanced polyester material will not dry out or fall off.

Sizes - Various sizes are available to suit the application.

Coloured - Available in white, clear and a variety of bright colours for convenient identification of tubes and samples.

MICROTUBE

TOUGH-TAGS

Standard Tough-Tags™ are manufactured in chemically inert polyester and have a strong adhesion to all plastics and other materials. Unlike conventional tape or paper materials, these labels will withstand steam-autoclaving, boiling water baths, freezing to -20˚C, plus brief contact with organic solvents and caustic agents without peeling.

Formats - Tough-Tags™ are available in rolls or in sheet form for the processing of large numbers of samples using a laser or inkjet printer.

TOUGH-SPOTS

™

Designed for all types of microcentrifuge tubes and vials, these Tough-Tags™ are supplied in rolls or in laser printer compatible sheets of pre-cut peel-off labels. Available in clear, white and a variety of colours in two sizes, small and large.

General Purpose Tough-Tags™ in white, are sized to fit a variety of laboratory containers, such as 50ml plastic centrifuge tubes or glass test tubes.

Teeny Tough-Tags™ are designed specifically for use with 0.2 / 0.5ml PCR® tubes and so will withstand thermalcycling, autoclaving, boiling water baths and freezing to 80˚C. Use the ultra-fine Teeny Writer Pen is ideal to write on these small labels.

These pre-cut, peel-off dots for microcentrifuge tubes and cryogenic vials are useable in boiling water baths at 100˚C, dry heat to 150˚C without cracking, peeling or degrading and down to -196˚C (for both vapour and liquid phase nitrogen). In 5 colours, to offer easy sample and tube identification, and in diameters 9.5mm,12.5mm, 19mm and 25mm, they are available in rolls or in laser printer compatible sheets of precut peel-off labels. All ToughSpots™ have the chemical and physical characteristics of standard Tough-Tags™.

CRYO-TAGS

™

The cryogenic series of Tough-Tags™ are pre-cut labels designed for the labelling of microcentrifuge tubes and vials for use at extremes of temperatures. These unique labels will withstand conventional freezing and cryogenic liquid nitrogen storage down to -196˚C (for both vapour and liquid phase nitrogen). Additionally, they are chemically resistant and will withstand boiling water baths at 100˚C and dry heat to 150˚C without cracking, peeling or degrading.

Cryo-Babies™ are 32.5 x 12.5mm in size and are designed for 1.5ml microtubes and standard cryovials, while the larger Cryo-Tag™ labels are ideal for identifying test tubes, bottles, flasks, vials and freezer boxes.

Both Cryo-Babies™ & Cryo-Tags™ are available in rolls or laser-/inkjet printable sheets for processing large numbers of sample labels.

ORDERING INFORMATION TTGP1000 TTS*1000 TTL*1000 TTGP1050 TSW2240 TTLW2016 LTTM1000 MISL1000

Tough-Tags™, White, General Purpose, 38 x 19mm, roll of 1000 Tough-Tags™, Small, *specify colour, 24 x 12.5mm, roll of 1000 Tough-Tags™, Large, *specify colour, 32 x 12.5mm, roll of 1000 Laser Tough-Tags,™ General Purpose, 37 x 18.5mm, 60 labels/sheet, 1500 Laser Tough-Tags,™ Small, 23.5 x 12.5mm, 119 labels/sheet, 2975 Laser Tough-Tags,™ Large, 31.5 x 12.5mm, 85 labels/sheet, 2125 Laser Teeny Tough-Tags,™ 23mm x 8mm, 154 labels/sheet, 3850 Laser Microscope Slide Tough-Tags™ Labels, 22 x 22mm, 96 labels/sheet, 2400

TTTNY TNYWTR

Teeny Tough-Tags,™ White, 21 x 7mm, 60 labels/sheet, 1500 Teeny Writer Pen, each

TTSWKK20 TTLWKK20 LTTI1000 BAKE250

Laser Tough-Tags,™ KissCut, 24 x 13mm, 160 labels/sheet, 3200 Laser Tough-Tags,™ KissCut, 33 x 13mm, 126 labels/sheet, 2520 Laser Tough-Tags,™ KissCut, 25 x 25mm, 80 labels/sheet, 1600 Bake-Tags,™ 38 x 19mm, 250

TSPOTS* TSPOT50* TSPOTS75 TSPOTS100

Tough-Spots™, Small, *specify colour, 9.5mm ø, roll of 1000 Tough-Spots,™ Large, *specify colour, 12.5mm ø, roll of 1000 Tough-Spots,™ White, 19mm ø, roll of 1000 Tough-Spots,™ White, 25mm ø, roll of 1000

SPOT1000* SPOT2000* SPOT3000 SPOT4000

Laser Tough-Spots,™ Small, *specify colour, 9.5mm ø, 192 labels/sheet, 3640 Laser Tough-Spots,™ Large,*specify colour, 13mm ø, 192 labels/sheet, 3640 Laser Tough-Spots,™ White, 19mm ø, 108 labels/sheet, 2160 Laser Tough-Spots,™ White, 25mm ø, 63 labels/sheet, 1260

CRY-1000 LCRY1700* LCRY2380* LCRY3020

Cryo-Babies,™ 32.5 x 12.5mm, roll of 1000 Laser Cryo-Babies,™ *specify colour, 33 x 13mm, 85 labels/sheet, 1700 Laser Cryo-Tags,™ *specify colour, 24 x 13mm, 119 labels/sheet, 2380 Laser Cryo-Tags,™ White, 76 x 51mm, 12 labels/sheet, 150

CRY-2000 LCRY1000 LCRY1100

Cryo-Tags,™ 38 x 19mm, roll of 1000 Laser Cryo-Tags,™ Cut to size, White, 216 x 297mm, blank Laser Cryo-Tags,™ Cryo Vial, White, 43 x 19mm, 52 labels/sheet, 1040

69

balances ANALyTICAL

nimbus Balances and scales from Adam Equipment meet the application needs of the laboratory, educational, industrial, retail and medical fields.

From research analytical balances to compact balances and industrial scales, Adam Equipment has been producing weighing scales, balances and mass measuring equipment for over 40 years.

• Backlit LCD display • Colour coded keys • Level indicator with adjustable levelling feet • Robust metal housing Model

Model

Capacity

• Sealed keypad • External/ internal calibration • Removable drag shield to 4 decimal Readability

Platform size

NBL 84e NBL 84i 80g 0.0001g 90mm Ø NBL 124e NBL 124i 120g 0.0001g 90mm Ø NBL 164e NBL 164i 160g 0.0001g 90mm Ø NBL 214e NBL 214i 210g 0.0001g 90mm Ø NBL 254e NBL 254i 250g 0.0001g 90mm Ø e models are external calibration; i models are internal calibration

Designed for scientists, the Nimbus series of balances offers 15 models that deliver a full breadth of precision readabilities ranging from 0.1mg to 0.1g.

Nimbus Analytical

NEW

Innovative design enables a reduction of the balance’s footprint, so it occupies less space but retains the highest level of performance. A single piece of extruded aluminium forms the balance base. The aluminium provides superior thermal transfer for a more regulated temperature, while the solid core is more rigid, offering greater stability and enabling highly repeatable results. The internal mechanism is fabricated using hardened materials, helping the Nimbus tolerate rigorous laboratory use. Crafted from a single block, the weighing sensor in the Nimbus contains fewer parts than a traditional force motor balance. Highly efficient mechanics plus improved processing power of the electronics leads to more accurate results.

nimbus precision Model

• • • •

Backlit LCD display Colour coded keys Sealed keypad Removable draft shield supplied on 0.001g readabilities

• Robust metal housing • External calibration • Level indicator with adjustable levelling feet

Model

Capacity

Readability Platform size

NBL 223e NBL 223i 220g 0.001g 120mm Ø NBL 423e NBL 423i 420g 0.001g 120mm Ø NBL 623e NBL 623i 620g 0.001g 160mm Ø NBL 823e NBL 823i 820g 0.001g 160mm Ø NBL 1602e NBL 1602i 1600g 0.01g 160mm Ø NBL 2602e NBL 2602i 2600g 0.01g 160mm Ø NBL 3602e NBL 3602i 3600g 0.01g 160mm Ø NBL 4602e NBL 4602i 4600g 0.01g 160mm Ø NBL 4201e 4200g 0.1g 160mm Ø NBL 6201e 6200g 0.1g 160mm Ø NBL 8201e 8.2kg 0.1g 160mm Ø NBL 12001e 12kg 0.1g 400×300mm NBL 16001e 16kg 0.1g 400×300mm NBL 22001e 22kg 0.1g 400×300mm e models are external calibration; i models are internal calibration

moisture analyser The PMB’s fast response time and easy-to-use functionality make it the ideal moisture analyser for a range of different applications. The automatic test setting function allows the user to quickly run multiple tests without additional input while the built-in memories allows data to be stored for future reference. The PMB offers smart features that have been developed to provide the ultimate performance for moisture analysis.

Model PMB 53 PMB 202

70

• Built-in memories for storing products and settings • 3 settings for heating sample fast ramp up, step and standard • Automatic test start setting for when the lid is closed • Date and time

Capacity

Readability

Platform size

50g 200g

0.001g /0.01% 0.01g /0.05%

3.9″ Ø / 100mm Ø 3.9″ Ø / 100mm Ø

• Pan lifter to easily remove samples • Full range tare • Zero Tracking • Quick and easy to configure through the keypad • Multilingual display • Large backlit display with dual text prompts • Capacity tracker • Non-slip adjustable levelling feet • Colour coded, sealed keypad • Large stainless steel pan • Overload protection • Splashproof to protect from accidental spills • Spirit Level • Single Halogen Lamp 400W • Calibration facilities for temperature and weight

core

compact • • • • • • • • • • • • • •

Best choice for simple operation and economy

CQT 202 CQT 251 CQT 601 CQT 1501 CQT 2601 CQT 2000 CQT 5000

Capacity

Readability

Platform size

200g 250g 600g 1500g 2600g 2000g 5000g

0.01g 0.1g 0.1g 0.1g 0.1g 1g 1g

120mm Ø 120mm Ø 120mm Ø 120mm Ø 145mm Ø 145mm Ø 145mm Ø

dune

CB

• • • • • • • • • •

Easy to use keypad Portable with battery power Stainless steel pan g, oz and lb

• 304 stainless steel removeable top pan • optional mains adaptor

Model

Capacity

Readability

Platform size

500g 1000g 3000g

0.1g 0.1g 1g

130mm Ø 130mm Ø 130mm Ø

CB 501 CB 1001 CB 3000

Model

compact

compact

• • • •

• Battery power (6 x AA Batteries) • Auto sleep / power down function to save battery life • AC Adapter

9 weighing units Full range tare Zero Tracking Simple 4 button operation Backlit LCD display Stainless steel pan ShockProtect™ overload threepoint protection Can be stacked for storage (120mm pan size only) Dual tare keys Lightweight and portable Modern low profile design Colour coded, sealed keypad Low cost and simple to use External calibration

3 Weighing units (g, lb, oz) Full range tare Zero Tracking Large easy to read LCD display Overload protection Colour coded, sealed keypad Lightweight and portable Simple user-friendly operation Precision load cell technology Splashproof to protect from accidental spills • External calibration • Low battery indication Model DCT 201 DCT 2000 DCT 5000

• Auto sleep / power down function to save battery life

Capacity

Readability

Platform size

200g 2000g 5000g

0.1g 1g 2g

145×145mm 145×145mm 145×145mm

highland portable • • • • • • • • • • • Ideal for basic lab work, field use and industrial applications

15 weighing units Zero Tracking Backlit LCD display Capacity tracker ShockProtect™ overload three-point protection Can be stacked for storage (120mm pan size only) Removable draught shield (120mm pan size only) Stainless steel pan Internal calibration weight Below balance weighing with hanger Colour coded, sealed keypad

• • • •

Internal rechargeable battery Auto power off Low battery indication DC Adapter

Model HCB 123 HCB 153 HCB 302 HCB 602H HCB 602 HCB 1002 HCB 1502 HCB 3001

Capacity

Readability

Platform size

120g 150g 300g 600g 600g 1000g 1500g 3000g

0.001g 0.005g 0.01g 0.01g 0.02g 0.01g 0.05g 0.1g

120mm Ø 120mm Ø 120mm Ø 120mm Ø 120mm Ø 120mm Ø 120mm Ø 120mm Ø

71

Contents • multiSUB horizontal gel systems

page 2

• gel visualisation

page 51

• runVIEW real-time gel electrophoresis

page 15

• gel documentation & analysis

page 52

• COMET assay

page 18

• rockers and shakers

page 56

• clinical electrophoresis, cellulose acetate

page 20

• hybridisation incubators

page 58

• UV safety cabinets (PCR)

page 23

• liquid handling/pipettes

page 60

• horizontal electrophoresis consumables

page 24

• microcentrifuges

page 62

• omniPAGE vertical gel systems

page 26

• thermal cycler

page 65

• heating baths

page 66

• microcentrifuge tube storage and chillers

page 68

• omniPAGE-DGGE vertical slab gel systems page 41

• labelling tapes

page 69

• blotting

page 44

• balances, top-pan and analytical

page 70

• membranes, buffers, stains

page 46

• power supplies

page 48

• gel drying

page 50

incl. mini, mini-wide, WAVE maxi and large format • isoelectric focusing and 2D omniPAGE

page 40

biocom

ltd 6 LOMOND CRESCENT, PA11 3HJ, UK

T

| +44 (0)1505 615973

F

| +44 (0) 1505 615976

E

| INFO@BIOCOMDIRECT.COM

W

| WWW.BIOCOMDIRECT.COM

For Terms and Conditions, please call. Please note that specifications and product colour are subject to change without notice.

© Biocom Ltd 2016

BRIDGE OF WEIR,