What is the 50K Chip?
available sources such as the bovine reference genome, Btau1, and the Bovine HapMap Consortium data set. All SNP probes have been validated in 19 common beef and dairy breeds. This product targets evenly distributed SNPs that are polymorphic across the breeds tested and provides an average probe spacing of 49.4kb and a median spacing of 36.9kb. ■ This 24-sample BeadChip represents the highest density genotyping solution for characterizing the genome in dairy and beef cattle ■ The PCR-free, single tube sample preparation significantly reduces labor and potential sample handling errors ■ Laboratory Information Management System (LIMS) and robotic automation are available to accurately and efficiently track samples throughout analysis Supporting the most comprehensive genome-wide genotyping studies, the 777,000 SNP BovineHD BeadChip expands the diversity of bovine breeds assessed in genetic prediction and enables more dis■ coveries of quantitative traits.
he BovineSNP50 BeadChip (50K Chip) is a multi-sample genotyping panel powered by Illumina’s Infinium HD Assay. This assay provides the industry’s highest call rates, allows for flexible content deployment, and enables the detection and measurement of copy number variation. The BovineSNP50 v2 BeadChip contains 54,609 highly informative SNPs uniformly distributed across the entire genome of major cattle breed types, empowering applications such as genomewide enabled selection, identification of quantitative trait loci, evaluation of genetic merit of individuals, and comparative genetic studies. This BeadChip was developed by Illumina in collaboration with the USDA-ARS, University of Missouri, and the University of Alberta. More than 24,000 SNP probes target novel SNP loci that were discovered by sequencing three pooled populations of economically important beef and dairy cattle using Illumina’s Genome Analyzer. Additional content is derived from publicly
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Relationships of Beef Breeds Using the 50K Chip by LARRY KUEHN, PHD, RESEARCH GENETICIST, U.S. MEAT ANIMAL RESEARCH CENTER reeds of cattle were established primarily through selection based on physical appearance (color patterns, stature), easily observed performance (milk production, muscling), or due to geographic constraints. These various selection pressures produced the uniform populations of cattle we define as breeds. Selection occurred over several generations; hence, breeds of cattle also diverged genomically due to selection pressure, inbreeding, and drift. Based on genomic differences, we should be able to determine the breed composition of animals of unknown origin. Knowledge of breed composition of cattle would be useful for predicting heterosis, evaluating adaptability to
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BlACK ANGUS READY FOR WORK
BULL SALE March 10, 2014
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Selling: 100 Yearling & Coming 2-Year-Old Angus Bulls
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Wayne Connell – Auctioneer Cattlemen’s Livestock Auction – Belen, New Mexico C A L V I N G
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Heartstone Angus, LLC J-C Angus U Bar Ranch Hartzog Angus Cattle AC BL K
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For catalog call 575/535-2975 or email dogilvie1@hotmail.com Remember: IT’S NOT BLACK HIDE, IT’S ANGUS INFLUENCE!
DECEMBER 2013
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P P.O. .O. B Box ox 10 10 Gila, G ila, N New ew Mexico Mexico 88038 88038 5 575-535-2975 75-535-2975 Home Home 5 575-574-4860 75-574-4860 Cell Cell