chromatography
26 Issue 6 2019
Know your ions Overcoming the limits of HPLC with ion chromatography
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hat’s the difference between High Pressure Liquid Chromatography (HPLC) and Ion Chromatography (IC)? What are the capabilities and limitations of each technique? Knowing these differences can actually make a huge difference. As a proven, robust and highly versatile technique, IC can help HPLC users extend their analytical capabilities tremendously. Our free white paper compares the two methods systematically, focusing on the strengths of ion chromatography. HPLC is one of the most powerful
analytical techniques in the modern laboratory. From research to manufacturing to legal, it is widely used in many sectors. However, there are some inherent limitations. Ionic and polar compounds pose a challenge. So do organic acids. Moreover, some analytes, such as fluoride and chloride, are not active in the UV range, making it impossible to determine them with a UV detector, the detection technique most commonly used with HPLC. Ion chromatography (IC), on the other hand, is ideal for the separation and detection of ions and polar molecules, both
organic and inorganic. This makes IC the ideal extension of HPLC, increasing the analytical capabilities of the modern laboratory tremendously. Over the last 30+ years, Metrohm has made Ion Chromatography a powerful tool for both research and routine analysis. Laboratories in the pharma, food and water sectors in particular benefit not only from the method itself but from many automated inline sample preparation and injection techniques. Samples with a high load of particles (e.g. wastewater), solid and even gaseous samples can be analysed fully automatically down to the μg/L range with superior reproducibility. Hundreds of IC applications are available free of charge from the Metrohm Application Finder and users are supported in more than 120 countries by application specialists from the local Metrohm sales organisations.
Aspirata invests in Metrohm IC system A
spirata is one of the leading laboratory service providers in Southern Africa, with custom-built, state-of-theart chemical and microbiological laboratories enabling them to provide a broad range of testing and examination services. Being a leading service provider in the establishment of safe and
Metrohm ion chromatography systems integrated in Agilent OpenLab CDS
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y controlling Metrohm ion chromatographs, Agilent’s new chromatography data system (CDS) OpenLab has become yet more powerful. The major benefit for the analysis is that users can now perform all their chromatographic applications on one and the same universal software platform. The integration of the Metrohm IC instruments is based on a dedicated driver, enabling users of Agilent’s OpenLab CDS to comfortably program, control and monitor all components of a Metrohm IC system in their familiar software environment. The requirements of the FDA CFR 21 Part 11 are met by OpenLab CDS, ensuring the full integrity and traceability of data and results. Metrohm IC instruments can be fully operated by OpenLab CDS. The combination of a Metrohm IC and Agilent’s single quadrupole MS gives the option to perform IC-MS applications in one software. OpenLab users profit now from Metrohm’s unique suppressor technology, the flexible liquid handling
with Dosinos, and the comprehensive Inline Sample Preparation Techniques. There are more benefits: Using just one software for everything saves time and money for training on different software and costs for validation will be reduced.
healthy living, working and recreational environments, Aspirata recently invested in a Metrohm Ion Chromatography system. The Metrohm 930 Compact Flex IC has two detectors, viz. 944 the UV/VIS Detector as well as a conductivity detector, and is coupled to an 858 sample processor that can handle up to 178 samples. Samples are analysed for volatile and non-volatile acids, as well as Chrome VI. The system allows Aspirata to exploit two of the Metrohm Inline Sample Preparation (MISP) techniques, viz. In-line Pre-concentration followed by Matrix
HPLC is one of the most powerful analytical techniques in the modern laboratory. From research to manufacturing to legal, it is widely used in many sectors.
METROHM SA (PTY) LTD Tel: (011) 656 1918 CPT: (021) 852 0213 DBN: (031) 265 0067 Fax: (011) 656 2698 078 643 7113 info@metrohm.co.za www.metrohm.co.za
Elimination, to assist in the low ppb detection levels of Chrome VI. Furthermore, samples that fall outside the calibration range are automatically logically diluted and re-injected without the analyst having to lift a finger, thereby greatly enhancing the lab’s output while saving time and money.
METROHM SA (PTY) LTD Tel: (011) 656 1918 CPT: (021) 852 0213 DBN: (031) 265 0067 Fax: (011) 656 2698 078 643 7113 info@metrohm.co.za www.metrohm.co.za
Liquid chromatography solutions for the food industry
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nalytical methods are essential to ensure product quality, execute regulations and to comply with food standards, specifications and labelling requirements in the food industry. The most important components that are analysed in the food industry can be divided into three groups: • Residues and contaminants • Food additives • Natural ingredients
High-performance liquid chromatography (HPLC) is one of the most important methods that is applied in all three fields. With a wide range of column materials and detector types, HPLC systems can be custom-made for the needed application. Purification Knauer is also one of the few companies to offer high-performance preparative HPLC solutions and continuous simulated moving bed (SMB) chromatography systems for the purification of food ingredients.
METROHM SA (PTY) LTD Tel: (011) 656 1918 CPT: (021) 852 0213 DBN: (031) 265 0067 Fax: (011) 656 2698 078 643 7113 info@metrohm.co.za www.metrohm.co.za
SEPARATION SCIENTIFIC SA (PTY) LTD Tel: (011) 794 4430 info@sepsci.co.za www.sepsci.co.za
