A Complete Guide on Pipettes and Their Different Types

A Complete Guide on Pipettes and Their Different Types

It is the goal of this blog to demonstrate the wide range of applications of some of the most common types of pipettes that may be found in modern laboratories or research institutions.

Pipettes are used in the laboratory to move fluids from one container to another quickly and precisely. Although many different types of pipettes are available, it is crucial to realize that certain pipettes are more accurate than others.

Let us now begin this informative guide to get some extra knowledge about this very important laboratory tool. From pipette technique to its different types, there is so much that we are going to learn.

Defining Pipette Pipettes are one of the most commonly used laboratory instruments. Small volumes of liquid can be measured and transferred between containers using these devices. You can find this equipment in different shapes and sizes; they range from basic serological and Pasteur pipettes to more complicated instruments that include a body, a plunger, and a piston, among other features. The latter is commonly referred to as micropipettes, and there are many different types of them, each with a unique set of characteristics.

Different Types of Pipettes 1. Graduated Pipettes Pipettes that are graduated are less accurate than volumetric pipettes. The serological graded pipettes, also known as "blow out pipettes," do not have zero marks. In contrast, graduated pipettes, sometimes referred to as "drain out pipettes," are designated with a zero at the beginning of their cone-shaped end.

2. Volumetric Pipettes In practice, volumetric pipettes are utilized by those exploring biochemical qualities and analyzing chemical reactions to perform their research. Nearly every school, institution, and professional laboratory has one or more of these machines on hand to perform the pipetting technique. They are well-known for their precision, and they can measure up to four significant numbers. Being available in various sizes allows scientists to precisely quantify the volume of a concentrated stock solution in the laboratory setting.

3. Pasteur Pipettes Pasteur pipettes are made of glass, as is the case with most medical instruments. These pipettes, with their bulb-shaped tops, are often mistaken for liquid droppers. Nowadays, Pasteur pipettes are regarded as being somewhat imprecise. It is more common to find them in biology laboratories than chemistry laboratories, where they are used to transfer aqueous solutions from one container to another. They are neither graded nor calibrated. Pasteur pipettes are named after the French physician Louis Pasteur.

4. Micropipettes With the help of micropipettes, scientists and professionals can obtain extremely accurate readings. It is recommended to calibrate micropipettes regularly – at the very least once every three to six months.

5. Vacuum-Assisted Pipettes Pipettes that are vacuum-assisted might be graded or volumetric in nature. The graduation marks on graded vacuum-assisted pipettes are numerous, but volumetric vacuum-assisted pipettes measure a single volume and have just one graduation mark. Typically, polystyrene, glass, or borosilicate is used in the construction of vacuum-assisted pipettes. However, they lack pistons and require suction.

Determine which pipette type is best for the test you perform before commencing your liquid measuring and pipette technique. Your results could be severely impacted if you use the wrong instrument or even the proper instrument poorly.

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