An introduction to the use of molecular genetics in plant breeding

Contact: Dr. Naglaa A. Ashry

naglaashry@yahoo.com

An introduction to the use of molecular genetics in plant breeding 1. Native PAGE 2. Native Gradient PAGE 3. Urea PAGE

4. SDS PAGE 5. SDS Gradient PAGE

6. IEF 7. 2D PAGE 8. Western Blot

An introduction to the use of molecular genetics in plant breeding

An introduction to the use of molecular genetics in plant breeding

An introduction to the use of molecular genetics in plant breeding

An introduction to the use of molecular genetics in plant breeding

An introduction to the use of molecular genetics in plant breeding

An introduction to the use of molecular genetics in plant breeding

An introduction to the use of molecular genetics in plant breeding

An introduction to the use of molecular genetics in plant breeding

An introduction to the use of molecular genetics in plant breeding

An introduction to the use of molecular genetics in plant breeding

An introduction to the use of molecular genetics in plant breeding

An introduction to the use of molecular genetics in plant breeding

Enzymes in DNA replication

Helicase unwinds parental double helix

Binding proteins stabilize separate strands

DNA polymerase DNA polymerase I (Exonuclease) removes binds nucleotides RNA primer and inserts to form new strands the correct bases

Primase adds short primer to template strand

Ligase joins Okazaki fragments and seals other nicks in sugar23 phosphate backbone