Posters Abstracts
P15 Draft of Polyplax serrata genome: a tool for population genomic inference Evolution of animal lice (Animal lice)
Citlali Gil Aguillon1, Jan Štefka2, 3, Marie Krausová2, 3 1 Undergraduate Program on Genomic Sciences, UNAM, Mexico 2 Biology Centre, ASCR, České Budějovice, CZ 3 Faculty of Science, University of S. Bohemia, České Budějovice, CZ Polyplax serrata is a sucking louse commonly parasitizing several species of Palearctic rodents, particularly those belonging to the genus Apodemus. Due to its wide geographical distribution and host spectrum, P. serrata has become a subject of several co-evolutionary studies that revealed varied patterns of population history and genetic distribution across European continent. Up to now, the resolution and inference of these studies were limited to a handful of gene sequences and microsatellite markers. To supplement these data, we provide a draft assembly of P. serrata genome. DNA library was sequenced by Illumina Miseq (paired-end 300 and 200 bp). Genome size was estimated, by kmer analysis, as being approximately 131 Mb. A preliminary assembly, for decontamination purpose, was performed with MEGAHIT, obtaining an almost complete genome of the symbiont Legionella. After decontamination using blobtools, reads were reassembled with SPAdes, obtaining an assembly of 240 Mb, average GC content of 37.98%, NG50 of 379 Kb and LG50 of 104 (largest scaffold spans 0.7% of assembly). BUSCO was used to assess the completeness of expected gene content, only 8.2% was missing and 2.5% fragmented, indicating a high quality of the assembly. In future, when extended with a transcriptome-assisted annotation, the genome assembly will provide a basis for population-genomic studies focusing on functional differences between P. serrata lineages adapted to different host species. It will also allow an in-depth analysis of gene flow across a transect in central Europe connecting two lineages in a secondary contact in hybrid zone.
The 6th International Conference on Phthiraptera
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