What are buffer tablets made of, and its Usage? Buffer tablets are mainly used to prepare buffer solutions for concentrations. You have to dissolve the tablet in distilled water or in 100 ml of deionized water. This will then produce a specified solution of certain pH which is ready for use. After dissolving the tablet in water at a specified temperature of 20 degrees Celsius, you will get a solution of pH 6.8 which is readily available for use.
BioGnost's Buffer tablets help produce phosphate buffer solutions that are stable in pH and have large buffer capacities and small temperature coefficients. They are simple to consume and dissolve. After the correct pH has been set, buffer tablets can be used for various histological and cytological procedures. These procedures require phosphate buffer solutions. In addition to their use in general haematology for blood smear counting procedures, they have frequently been used to practice cytology. Buffer tablets have been used in a lot of laboratory practices and are used to treat various health issues.
How to Prepare a Buffer Tablet Solution? The phosphate buffer solution is prepared by dissolving the tablet in a small amount of distilled water, which should be added drop-by-drop to the tablet. The pH should be checked and corrected with 0.1M NaOH if necessary. The resulting solution will be clear with a pH of 7.2 ± 0.2 and osmolarity of approximately 300 mOsmol/l (3g/dl). The final volume should be approximately 2mls to minimize loss by evaporation during staining procedures. Consumption of the buffer tablets is simple and requires no special equipment or skills; dissolve them in water and add them to the cells to be processed. The tablets should be stored in a cool, dry place and away from direct sunlight.