Portafolio SIU. Sede de Investigación Universitaria. English

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Specialized Services

Information and contact Phone: (57) (4) 2196402 Email: siu@udea.edu.co Pedestrian gate: Cll. 62 N 52-59 Vehicular gate: Cra. 53 N 61-30

/SededeInvestigaciรณnUniversitariaSIU @SIUUdeA siu_udea

SIU - Central square Author: Jose Alfredo Betancur

University Research Headquarters


ANIMAL

FACILITY


Services

The Animal Facility is a place where laboratory animals are kept and bred under suitable macro and micro environmental conditions depending on a species. One of our highlights is that we generate characteristics that guarantee perfect SPF- specific pathogen free colonies. This allows us to count on the best animals in the country, which is the most ample variety that includes even transgenic animals. All our research work is done hand in hand with various research groups applying an ethical framework pursuant to Law 84 of 1989, “The national statute for animal protection” and under the auspices of

•Scientific and technological management of the field of experimentation with conventional animals and pathogen-free animals. •Maintenance of different animal species (Observation, changing of bedding, cage cleaning, and feeding, among others). •Washing, disinfection and sterilization of Animal Facilities-user equipment and materials. Application and Research Areas •Experimentation field for SPF animals •Experimentation field for conventional animals Some Application Fields •Tropical Diseases •Immune Diseases •Respiratory Diseases •Neurology •Toxicology •Regeneration •Microbiology Main Technology Installed •Autoclave sterilizer


Equipment

Calibration


The equipment calibration laboratory belonging to the SIU has a team of highly specialized in calibrating pipettes, balances, digital burettes and dispensers. Today our services are offered to internal and external users to the University. Formed since 2009 and accredited in 2012 by the ONAC in the volume variable, the laboratory has the capability to ensure high reliability of the equipment involved. The calibration service of measuring equipment consists to determine the error and measurement uncertainty to be attributed to the results for a given instrument following an internationally standardized method under specified conditions. Benefits of Calibrating Measuring Equipment The use of measuring equipment, the mechanical stress it bears, aging and wear of its components along with inadequate manipulation, gradually deteriorate equipment functions. When this happens, the results of the measurements made using this equipment begin to become less trustworthy. These types of situations may be avoided through maintenance, calibration and adjustment whenever required. The fact that measuring equipment may be new and seem reliable does not necessarily guarantee that the equipment’s measurements will be accurate. Hence,

Services •Calibration of adjustable volume single-channel micropipettes •Calibration of adjustable volume multichannel micropipettes •Calibration of burettes •Calibration of Dispenser •Calibration for Balances Application Fields •Biotechnology •Biodiversity •Biology •Genetics •Chemistry •Pharmaceutical •Food and General Industry •Clinical Laboratories •Environmental Impact •Health Sciences •Agricultural Sector Main Technology Installed •A Sartorius ME36S 6 decimal place micro-balance •A Sartorius CPA225D 5 decimal place semi-micro-balance •A UNIT-UT325 Thermometer •An Extech RH520 Data logger to measure humidity and


Mauricio Rojas Lรณpez


The flow cytometry laboratory is characterized by its support for Universidad de Antioquia research groups and the community in general to conduct research and extension projects which require an analysis of cells using high quality scientific - technical criteria. The Cytometry Unit supports different undergraduate and postgraduate Universidad de Antioquia programs providing technical service, data analysis and standardization of new procedures for researchers and students executing projects. The Cytometry Unit also provides services to the community offering technical analysis of cell populations required to support diagnostics of different pathologies and offering flow cytometry training courses. Services •Courses and theoretical-practical training on cytometry and technology installed. •Technical assistance in Flow-cytometry-related projects. •Stem cell count (CD34+/CD45+) required for hematopoietic stem cell transplantation. Monitoring of specific lymphocyte populations: •cd3 cd4 cd8 lymphocytes •HLA B27 including B7 which provides cross-reactivity in the presumption of ankylosing spondylitis •Lymphocytes T and B (CD3/CD19) •Total Lymphocytes T (CD3) •Total Lymphocytes B (CD19) •Lymphocytes B (CD20)

•Stem cells (CD34/CD45) •Monocytes (CD14/CD16) •NK Cells (CD56/CD16) •Separation of cell populations •Aseptic separation •Cell cycle in fixed samples (PI without a standard of chicken erythrocytes and ram Thymocytes) •Viability and apoptosis (Annexin V and 7 AAD) •Mitochondrial and cell damage (DIOC and PI) •Alignment verification Application Fields •Pharmacology •Inmunovirology •Immunodeficiencies •Reproduction •Tropical Diseases •Marine Biology •Cell Immunology •Immunomodulation •Biotechnology •Basic Biomedical Sciences •Solid organ transplantation Technology Installed •Coulter EPICS XL •FC-500 •MOFLO XDP (It enables the electromagnetic separation of cells for various purposes) •FACS Canto II Becton Dickinson •BD LSRFortessa™ Becton Dickinson


Mass Spectrometry Laboratory

CĂŠsar Segura Latorre


The Laboratory of Mass Spectrometry has two aims: offers high quality training in the technique and provides either qualitative and quantitative analysis as well as identification of several substances such as pesticides, pharmaceutical, peptides among others. The LEM has contributed to the top level training in this technology at Universidad de Antioquia and in the city. Currently, we are advancing in acreditation under the international standard ISO 17025.

Quantitative analysis Quantification of substances of interest in pharmacy and environmental sciences Application Fields •Pesticides •Pharmaceutical industry • Natural Products • Food Products Technology Installed

Services •Analysis of simple mixtures •Determination of molecular mass •Purity control of chemical substances • Targeted analysis • Low resolution metabolomics • Organic compounds analysis Application Fields Qualitative analysis •Determination of molecular mass •Control of spectral and chromatographic purity

•An Agilent Technologies 1200 High Pressure Liquid Chromatography (HPLC) connected to a single quadrupole Agilent Technologies Model VL mass spectrometer with electrospray ionization (ESI) sources and atmospheric pressure chemical ionization (APCI).


Physical Properties Measuring System Oscar Luis Arnache Olmos

Telephone: (574) 219 6575 e-mail: oscar.arnache@udea.edu.co


The Physical Properties Measuring System- PPMS is state-of-the-art technology made by Quantum DesignTM for the characterization of materials. It is completely automatized with high precision in the variation of magnetic fields and temperatures and has multiple functions as:

Technology installed

This equipment is used in research and as academic training tool for master’s and doctoral students to supply their technological needs found in the nation’s setting via extension services.

•A 6000 model console •Vibration Sample Magnetometer - VSM - Module •Option ACT - for electrical transport measurements •Option Cp - for heat capacity measurements •Dewar Tank •CAN (control area) •Cryomech- liquefaction system •MultiVu Software •Electrical Backup Systems - UPS

Services

Application Fields

•Automatic measurement of magnetic, electric and heat properties in relation to temperature (from 4.2K to 400K) and of the external magnetic field applied (0 a 9T) having high precision and control. •Measurement of the Electrical transport properties of materials (I vs V Curves, Hall Effect, AC and DC Resistivity). •Measurement of the electrical resistance in relation to temperature and a magnetic field. •Measurement of heat properties via a heat capacity analysis (Cp) of a solid. •Measurement of magnetic properties of solid systems or to determine various magnetic behaviors.

•Biomedical Applications •Magnetic Polymers – composites •Nanoparticles – micelles •Catalysis – sensors- decontaminants •Magnetoresistance •Superconductivity •Electronics

•DC/AC Magnetization •(DC/AC) Electrical transport (Resistivity, Hall Effect, I vs V Curves, Critical Current) •Heat Capacity


Juan Fernando Alzate Restrepo

cnsg@udea.edu.co


Services

The National Center for Genome Sequencing is a project of the Universidad de Antioquia Research Headquarters, the Science, Technology and Innovation Administrative Department COLCIENCIAS (in Spanish, Departamento Administrativo de Ciencia, Tecnología e Innovación-) and Products Roche S.A. Company. The mission of the Center is to provide state-of-the-art tools to get to know, protect and value our biodiversity, to introduce a robust physical infrastructure in the National Science, Technology and Innovation System to conduct projects to discover genetic information of Colombian biological resources. Moreover, it will be able to respond to a demand for genome sequencing of sectors that require it at a national and international level.

•Pathogen classification methods and attributable genetic characteristics •Taxonomic classifications •Bioprospecting studies which allow the genetic evaluation of characteristics related to different industrial projects •Studies of new organisms that allow to perform species characterization, developing biological inventories •Extraction of nucleic acids •Genome sequencing, metagenomes and amplicon libraries •Bioinformatics of genomic assembly data •Automatic annotation of genomes •Assistance and complementary services for project development in the fields of molecular biology, genomics and metagenomics Application Fields •Biotechnology •Biodiversity •Environmental impact •Agricultural sector Installed Technology A complete laboratory equipped to perform any type of test for molecular biology, genomics, metagenomics or transcriptomics, and to conduct further computational analysis.


SCANNING ELECTRON MICROSCOPY

Dayana Meza Terraza

Telephone: (574) 219 66 10 - (574) 219 66 15 e-mail: laboratoriosem@udea.edu.co


A Scanning electron microscope – SEM- is one of the most versatile tools modern science used by the most to evaluate surfaces to study the composition and morphology of solid organic, inorganic and biological materials. An image formed based on electrons (electronic microscopy) has greater depth of field and resolution than one created using a light beam (optical microscopy) allowing you to obtain tridimensional images to help all studies related to a superficial analysis. The SEM at Universidad de Antioquia has a 3 nm high-vacuum resolution and a magnification of up to 300,000X. Also, it has an X-ray microprobe which detects elements that have an atomic weight higher than Beryllium, and a 0.1% resolution in weight. Services •Micro structural analysis of ceramics, metals, polymers, and compounds •Semiquantitative elemental chemical microanalysis (punctual, in a geometrical zone and the line and zone mapping of elements) •Failure analysis •Analysis of corrosion and the deterioration of materials

Applications Archeology Botany Agricultural sciences Natural sciences: -Chemistry -Physics -Biology Forensic sciences Materials science: -Corrosion -Failure analysis -Crystallography analysis -Nanotechnology -Tribology -Coatings -Paints -Pigments -Materials (ceramics, polymers, metals, compounds) -Mines and metallurgy -Mineralogy Geology Industries:

-Food -Pharmaceutics -Metal mechanical industry -textile Medicine Odontology Pathology Toxicology Technology installed JEOL JSM 6490lv scanning electron microscope with high-low vacuum operation modes. It currently has the following accessories: -Secondary electron detector (SEI) -Backscattered electron detector (BES) -X-ray microprobe (EDX) -Secondary electron low vacuum detector (LVSEI) -GATAN H1004 hotplate -Deben CoolStage MK3 cooling plate -Pattern generator for nanolithography -Denton Vacuum Desk IV gold and graphite deposition system -A Samdri-795 critical point dryer


Johana Arboleda Telephone: (574) 219 66 81 - (574) 219 66 15 e-mail: laboratoriotem@udea.edu.co Echavarria


The Transmission Electron Microscopy (TEM) Laboratory at Universidad de Antioquia is an infrastructure dedicated to the high-resolution study of all types of materials, providing services to postgraduate and undergraduate Universidad de Antioquia’s students and external users. Services •Hygh resolution analysis of the internal morphology of all types of materials by Transmission Electron Microscopy using TEM and STEM modes. •Hygh resolution analysis of the crystalline structure of materials using TEM through electron beam diffraction. •Hygh resolution analysis of the elemental chemical composition of all types of materials using TEM with an EDS technique. •Preparation of all types of samples for TEM observation. •Processing of (human and animal) tissues for TEM (fixation, post-fixation, inclusion in resins, semi-fine, ultra-fine cuts, contrasting). •Processing of kidney biopsies for TEM with image interpretation and processing complemented with studies on conventional optical microscopy, immunofluorescence and immunohistochemistry (service working with the Department of Pathology at Universidad de Antioquia’s Medical School). •Processing of biopsies including heart, nerves, muscles and other organs.

TEXTO PENDIENTE

Technology installed •FEI TECNAI G2 F20 STWIN Transmission Electronic Microscope. •Fischione Plasma Cleaner Model 1020. •Leica EM UC7 Ultramicrotome •Glass Knifemaker Leica EM KMR3 •Leica EM AC20 Automatic Contrasting System •Gatan Ion Milling, PIPS II System •Gatan Dimple Grinder •Gatan Ultrasonic Cutter •Gatan Disc Punch System •Gatan Disc Grinder System Application Fields. •Nanotechnology •Health sciences (Human and animal) •Biological sciences • Material sciences


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