PRODUCT DATA SHEET
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SHEEP BLOOD AGAR BASE, MODIFIED PLATE
TMP 018
INTENDED USE for cultivation and studying haemolytic reactions of Bacillus cereus in accordance with ISO 21871:2006.
COMPOSITION Ingredients
Gms/Ltr
Enzymatic digest of casein
15.000
Agar
12.500
Sodium chloride
5.000
Enzymatic digest of soya
5.000
PRODUCT SUMMARY AND EXPLANATION Sheep Blood Agar Base, Modified with added sheep blood was developed to allow maximum recovery of B. cereus without interfering with their haemolytic reactions. This medium is formulated in accordance with ISO. It was formulated to be compatible with sheep blood and give improved haemolytic reactions of organisms. Bacillus cereus is Gram -positive aerobic or facultatively anaerobic, motile, spore forming, rod shaped bacterium that is widely distributed environmentally. B. cereus is associated mainly with food poisoning it is increasingly reported to be cause of serious and fatal non- gastointestinaltract. Hemolysis is very useful for differential characteristic that is best viewed when a bright light is transmitted from behind the plate. Four different types of hemolysis can be described: 1. Alpha-hemolysis (α) – Partial hemolysis that results in a greenish discoloration around the colony. 2. Beta-hemolysis (β) – Complete lysis of red blood cells resulting in a clear zone around the colony. 3. Gamma-hemolysis (γ) – No hemolysis resulting in no change in the medium. 4. Alpha-prime-hemolysis (α′) – A small zone of complete hydrolysis that is surrounded by an area of partial hemolysis.
PRINCIPLE Medium contains nutritional components like enzymatic digest of casein, enzymatic digest of soya and the addition of sodium chloride provides an osmotically balanced medium for bacterial Manufacturer Address: A- 902A, RIICO Industrial Area, Phase III, Bhiwadi-301019. Corp office Address: 903-909,9th Floor, Bigjos Tower, Netaji Subhash Place, Delhi-110034.
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