CleanShield Disinfectant folder 2018 (English)

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CLEANSHIELD DISINFECANT 100% biodegradable USA Preservative Test Test Method Summary: Duplicate 50g samples of prepared test solutions were challenged with either a mixed bacterial inoculum (E. coli, P. aeruginosa and S. aureus) or a mixed fungal inoculum (C. albicans and A. brasiliensis) to target approximately 105 – 106 organisms/mL of sample. The inoculated solutions were sampled immediately (Day 0) and after 3 days and 7 days of incubation at 25-30°C. The percentage and log reduction for the test results were determined as compared to an initial suspension control. Test Solutions: CleanShield Disinfectant Lot 20102010 prepared at concentrations of 1%, 2% and 4% in sterile deionised water Initial Suspension Control Results: Bacterial Inoculum: 8.5 x 105 CFU/g of test material Fungal Inoculum: 1.4 x 105 CFU/g of test material

All other controls performed as required by the testing documents. In reviewing these data, it is evident that there is a preservative effect against bacteria at all concentrations evaluated and some preservative effectiveness against fungi, most notably at the 4% concentration. We feel the best next step for you is to evaluate this preservative (D01) at these three concentrations in the presence of both clean and contaminated solutions of 40% HSC. This should help identify whether or not the bloating is caused by inadequate preservation in the presence of HSC and in addition, if the bio-burden associated with some batches of HSC can be tolerated. Sincerely, Scott

Scott Steinagel, B.S. Director, Microbiology Operations, ATS Labs 1285 Corporate Center Drive, Eagan, MN 55121


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