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Hi-Res Standard Agae Dyes
2.3.0 DNA Quality For each individual DNA sample 2µl of DNA, 8µl of deionised water and 2µl of 6x blue/orange loading dye (ThermoFisher) was added to a microcentrifuge tube and mixed using the pipet. The DNA solutions were then refrigerated while a 2% agarose gel was made. Initially 2g of Hi Res standard agarose (Bioproducts Ltd. Yorkshire, UK) was weighted out on a balance (Denver Instrument S 203) and added to a 250ml conical flask. Once this was completed 98ml of deionised water was added and heated on a hot plate (Halogen Hotplate HT2). The flask was continuously heated and stirred until the agarose powder had fully dissolved. Then the solution was cooled by running the base of the conical flask under water while stirring. Once the solution had begun to cool 2ml of 50X Tris acetate EDTA (TAE) (ThermoFisher) and 10µl of Gel Red Nucleic Acid Stain 10,000X in DMSO (Biotium. Fremont, USA) via pipetting. Once mixed the solution was poured into gel electrophoreses tank including well comb and left to set for 30 minutes. Once set the gel is immerged in approximately 300ml of 1x TAE, then 10µl of DNAsolutions including a PCR sizer 100bp DNA ladder (Norgen Biotek Corporation. Thorold, Canada)... Show more content on Helpwriting.net ... Buckinghamshire, UK) were used within each supplied tube. Each bead contains the desired ingredients needed for the successful PCR reaction, stabilizers, bovine serum albumin to increase overall yield, four deoxynucleotides triphosphates (dNPT), adenine, guanine, cytosine, tyramine ATP, approximately 2.5 units of puReTaq DNA polymerase and a reaction buffer. When reconstituted to the final volume of 22µl, the final volume of each dNTP is 176µM in 8.8mM of Tris HCL which at room temperature has a pH of 9.0. Finally, 44mM of potassium chloride and 0.66mM of magnesium chloride. All PCR was preformed within a fume cupboard (Bassaire 06HB) to minimise the risk of either moisture or genomic
Krebs In Soldier s Home By Ernest Hemmingway
After reading Soldiers Home by Ernest Hemmingway, Krebs seems to play an awkward character after returning home from the war. He puts himself distant from everyone including his mother and sister. The only person he would speak to would be his friend from the war, Keith. Krebs s father doesn t let him use the car before Krebs left to war because his father used to drive his clients in the vehicle for his real estate job. Also Krebs s father was very strict with giving permission to anyone using the car including his own wife. To fast forward when Krebs returns home from the war and a couple months past by and Krebs still doesn t know what to do with his life yet. Krebs s father decides to let Krebs use the car to go enjoy life and hopefully
Picasso Female Complex
Pablo Picasso: The Female Complex Many artists develop their works from their most compelling moments in life, whether they are filled with excruciating pain or unspeakable joy. Pablo Picasso creates work based on a multitude of influences in his life, from the suicide of his best friend to the rising of his career (Bio). However, a single theme reoccurs throughout his life that most would argue causes him to create some of the greatest paintings of his time: women. Critics shaped the different phases of his art into periods such as the Blue Period, Rose Period, CubismPeriod, Surrealist Period, and Self Portrait Period (Bio). His art was manipulated based on the dominant state of mind he was in during these stages of life, and many of the emotions had to do with the women he was involved with at the time. Though one might think that an artist who places such a passionate focus on women must deeply admire and respect them, Picasso does not fit into this standard. He actually felt a very strong sense of superiority to females, painting them as objects: cold, faceless, and purely created for sexual satisfaction (Withers 1). However, evidence strongly suggests that he probably feared them more than anything else. Every single one of his periods of art are heavily ruled by paintings of women, particularly nude, that tell a story about an experience he had; if he was so affected by women that he spent countless hours painting about it, there had to have been more going on
Transcendentalism
Transcendentalism was a movement that started in the 1830 40s which valued concepts such as the ideas of carpe diem, non conformity, and finding one s own beliefs. The idea of transcendentalism is present throughout the movie Dead Poets Society, directed by Peter Weir, and in the essay Walden, written by Henry David Thoreau. In Dead Poets Society, the idea of transcendentalismis demonstrated through a group of teenage boys going to an all boys school, being taught by an eccentric English teacher. The same concepts are also shown in the piece, Walden, this essay is a series of journal entries written during a two year stay in the woods near a pond. Both pieces of work exemplify the necessity of enjoying life and living it to the fullest through the transcendentalist elements of carpe diem and non conformity.
In order to live life and enjoy it to the fullest, one must seize the day, more commonly known as carpe diem. During the film Dead Poets Society, Mr. John Keating, the English teacher at Welton Academy, teaches his students the idea of carpe diem, meaning he wants them to go out and make their own choices and make the most of what they ve been given. One student, Neil Perry, decides to demonstrate this idea by trying out for a local play. He knows that his parent will not approve but despite that, he tries out anyway because it is something that he wants to do. Towards the beginning of the movie, Neil listens to his father when he is told that he must quit being
Human Interactions And The European Union
Interests
Interests consists of anyone who have some impact due to an issue. It can also include special interest, activity and advocacy group. All the interests mentioned in this section will cover the various issues mentioned in the above sections. Some of those interests are their customers, pension fund executives and managers, Oracle media representatives, employees, Oracle community (blog, users and user communities), United States Air Force, database administrators, database developers, vendors, Oracle technology writer/analyst/commenter specializing in enterprise software, etc. Also included are, other similar technology corporations watching Oracle`s next steps, the Attorney General, government officials, Department of Justice, ... Show more content on Helpwriting.net ... Solicitor General, Judiciary (Court Federal, State and District Court), news media, social media, client or vendor opinions and their sentiments. Also included are international institutions like the European Union (EU). (Kendall, and Ovide, 2015)
Information
Information refers to the related interests and concerned members of institutions is aware of an issue. It also includes the consequences each options available as an alternative. Issues are often contested (by lawsuits or social pressures), as interests have conflicting or different information. Information leads to progress or resolution of issues. Oracle had initial information about the violations, and was able to gather all additional information and provide relevant proofs. The proofs help to resolve or advance the lawsuits. Those could include listing the specific sections of the law violated, and log/trace files (technical information) proving illegal activities done via. Oracle`s website as in the case of illegal downloads. Information was also provided about the contract breach, steps taken for a hostile takeover and its justifications. The information is provided from various sources. Some of the other interests include activists, government institution, media, more information on the Rimini Street issue and infringement issues with Oregon.
Issue Rimini Street`s illegal conduct
Any issue that threatens Oracle`s reputation should be taken seriously. Issues tarnish firm`s reputation. As people have
Examples Of Optimism In Candide
Folly of Optimism There was a lack of good reasoning in hopefulness and confidence about the future in Voltaire s novel Candide. In his novel, Voltaire s character Pangloss is responsible for the novel s most famous idea: that all is for the best in this best of all possible worlds . A idea that is instill into the main character Candide. This optimistic idea is the main target of Voltaires satire that is based on the philosophy of other enlightenment thinkers. Voltaire does not like the idea and mocks the is\dea throughout his novel to create a statement.Voltaire characters follow this theme and their optimism is ruined as the horror and tragedy show a greater reality. In Candide, the protagonist remains optimistic for most of the novel, but after all his travels his views transformed. Candide is one of the few optimistic people in the story when he was surrounded by mostly pessimism. The utopian land of El Dorado teaches Candide that the world is not a perfect place. When he ... Show more content on Helpwriting.net ... Voltaire uses critical attitude with wit and humor in the philosophy presented in the novel. The satirical references to the best of all possible worlds involves the conflicting of what God had planned for them for a greater good and the realities of the natural catastrophes. Voltaire does not accept that, a perfect God (or any God) has to exist, (Shmoop.com) he mocks the idea that the world must be completely good through Candide and his journey. He makes Candide to always be going through a tragedy that there was no happy ending till the end where they cultivate their garden . Which represented through the satire meaning to avoid the world around them and focus on there self. That they live a time without sin as adam and eve who lived happily in there garden before there sin.Thus, how Voltaire show his own philosophies to be contradicted with the overall of how
Criminal Responsibility in Individuals with Dissociative...
A significant and controversial issue within the legal system is the insanity defense in which during a criminal trial, the defendant will make a claim that they are not guilty by reason of insanity, or in other words, they have deficient and impaired cognitive and mental capabilities. These mental health problems associated with insanity are caused by psychopathological disorders, which may have led to their dysfunction. What separates this from a regular plead of diminished capacity is that a plea of insanity is a full defense rather than just a partial defense (Legal information institute, n.d.). With the diminished capacity defense, the defendant s mental competence is still the focus, although they are pleading to a lesser crime... Show more content on Helpwriting.net ... Though the individual may or may not be aware that the other personalities exist, they will have knowledge of their amnesic episodes because of the gap in their memories in which they had no awareness. Despite the extremely rare occurrence of DID, because of its unique characteristics, it is well known in society through many of its portrayals in media. A famous example is the novella, Strange Case of Dr Jekyll and Mr Hyde, written by Robert Louis Stevenson, which portrays a character possessing two distinct personalities that are in conflict, one that is evil and the other that is good. Because of the increased interest in DID generated by Jekyll and Hyde, the split personality seen within this character has become almost symbolic of the multiple personalities seen in DID. There have been many other instances where multiple personalities have been portrayed in popular culture. It can become quite exaggerated at times concerning the symptoms and how the disorder develops in the first place, but there is an underlying truth behind how serious of an illness it can be. If there s one thing Hollywood and popular media has
United States Postal Service In Flying Like An Eagle
For over two centuries, people relied on the United States Postal Service (USPS) as a way to receive their letter and packages from people around the country. It is their only way where a person can obtain a parcel when there are no technologies to assist them. Nowadays, in the modern world, there are different kinds of ways where one can receive a package or a letter, from an email or other companies. This results in the USPS decreasing its values. In order for the USPS to keep up with the fast paced society, it should consider changing their ways of working. Before when automations were nowhere to be seen, people demand USPS to deliver their mails everyday. Sometimes when there is a large package, it might take weeks for the letters to reach their destination. So retailers assist delivering the packages in a more efficient way. In order for the USPS continue to be on... Show more content on Helpwriting.net ... In Daniel Stone s editorial article, Flying Like an Eagle , he advises the USPS some guidance to boost up their profit and their usage to the community. One of his inputs on the
The Importance Of Honesty
Honesty
Would you call yourself honest if you never have the chance to be dishonest? Honesty is the foundation upon which a person builds any relationship with their loved ones such as family or friends. Being honest to loved ones, will make them feel that they are trustworthy and close enough for to reveal their feelings. Honesty plays an important role in raising children to be good citizens. When I was six years old, one spring afternoon I was waiting for the bell to ring to go home from school. The Principal, Mrs. Garcia came to my classroom to give us a last minute announcement. I do not remember what she was talking about, because my mind was set on packing up and ... Show more content on Helpwriting.net ...
I was hoping my mom wanted to keep it, after all I was the owner. I found it, not my mother. Then she said, Tomorrow we will go to school and give it back to whoever this money belongs to. Tomorrow came and there we were, in the Principal s office. I remember the reaction of the Principal. She was crying like a river, kissing my mother, and thanking her over and over for her honesty and the great example she was to me. At that the moment I understood why she wanted to give the money back. It belonged to the Principal who put the folder on my desk, while she was giving the last minute announcement the day before. I had been in a hurry and I shoved the folder in my back pack by accident. My mother never told me that what we did was called honesty. We never talked about the incident. I learned by her example what the right choice was, and it changed my life forever. Shakespeare once said, Honesty is the best policy. If I lose my honor, I lose myself. From his quote, it is clear that Shakespeare thinks honor is more important than anything else in life. Honesty is a virtue that is uniquely valued by human beings as well by our Heavenly Father. Being honest often requires courage and sacrifice, especially when others try to persuade us to justify dishonest behavior.
Being honest ha Pros and Cons and some of them are the following: Pros: People respect you and trust you. They know you are a person with integrity. People want to be your
