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ItisintendedpurelyasabriefintroductiontothetechniqueanditisThermoSpectronic'sUV-visiblespectrometerscanbeusedtomeasuretheabsorbanceofultra violetorvisiblelightbyasample,eitheratasinglewavelengthorperformascanoverarangeinUV/VisSpectrophotometryandUVImaging.Definitionsand UnitsUltraviolet-visible(UV-vis)spectroscopyisusedtoobtaintheabsorbancespectraofacompoundinsolutionorasasolidemberAuthors:AlaaFrak HussainItisintendedpurelyasabriefintroductiontothetechniqueanditisThermoSpectronic'spolicytocontinuallyaddtothisrangeofdocumentationfor furtherdetails,astheybecomeavailableThereferenceUltravioletSpectroscopyUV-VISIBLESPECTROMETRYAbstractThetechniquecanbeusedboth quantitativelyandqualitativelyDownloadchapterPDFBasicPrinciplesofUltraviolet-VisibleSpectroscopyUV/Visspectroscopyisameasurementtechniquein whichtherecordingoftheabsorptionspectraofdifferentsamplesusingultraviolet(UV)andvisible(Vis)lightisachievedbyThisdescriptionoutlinesthebasic principlesforthosenewtoUV-Visiblespectrometry.TheUVregionrangesfromtonmandthevisibleregionfromtonm.Ultraviolet-Visiblespectroscopymakes useofradiationintheUltraviolet(UV)andVisible(Vis)radiationregionsoftheelectromagneticspectrumfromapproximately–nm(50,–12,cm 1)[]an instrumentabletomeasurethespectrumofasampleintheUV/VisrangeThisdescriptionoutlinesthebasicprinciplesforthosenewtoUV-Visiblespectrometry ØSuchDescriptionofProcessUV-VisibleAswithanyspectrometricdevice,aUV-Visspectrophotometermustbequalifiedforbothwavelength(x-axis)and photometric(y-axis,orsignalaxis)accuracyandprecision,andthefundamentalparametersofstraylightandresolutionmustbeestablishedUV/Visspectroscopy isameasurementtechniqueinwhichtherecordingoftheabsorptionspectraofdifferentsamplesusingultraviolet(UV)andvisible(Vis)lightisachievedbya spectrophotometer,ieWhatisactuallybeingobservedspectroscopicallyistheabsorbanceoflightenergyorelectromagneticradiation,whichexciteselectrons fromthegroundstatetothefirstsingletexcitedstateofthecompoundormaterialUV-visiblespectrometerscanbeusedtomeasuretheabsorbanceofultraviolet orvisiblelightbyasample,eitheratasinglewavelengthorperformascanoverarangeinthespectrumUniversityofKerbalaCollegeofScienceDepartmentof ChemistryTheultraviolet–visiblespectroscopy(UV-Vis)utilizeslighttodeterminetheabsorbanceortransmissionofachemicalspeciesineithersolidorPDFIn thisapplication-orientedbookletthefundamentalsofUV/VISspectrophotometryarefirstexplainedbeforefocussingonitsmainuseinFind,readandciteallthe researchyouArticlePDFAvailableJesperØstergaardAbstractThepresentchapterdescribestypicalinstrumentationandprinciplesofUV/Visible spectrophotometryAswithanyspectrometricdevice,aUV-Visspectrophotometermustbequalifiedforbothwavelength(x-axis)andphotometric(y-axis,or signalaxis)accuracyandprecision,andAspectrometercollectsthetransmittedlightandcomparestheoutputagainstabaselinemeasurementthatisreferencedas %transmission(I0).ØUVlightcanbeabsorbedbymoleculestoexcitehigherenergy(mostlooselybound)electronsfromlowerenergystatestohigherstates.