CENTRO DI RIFERIMENTO ONCOLOGICO
Technology Transfer Opportunities Contact: Ermes Mestroni emestroni@cro.it
Monoclonal Antibody - hEMILIN1 CRO Aviano researchers have developed a monoclonal antibody to an extracellular matrix (ECM) glycoprotein named EMILIN1 (Elastin Microfibril Interface Located proteIN), which has been shown to be expressed in smooth muscle and other mesenchymal tissues. This antibody has been successfully used in detection ELISA, in functional blocking the integrin interaction, immunohistochemistry and immunoblot assays. EMILIN1 interacts with the α4β1 integrin via the C-terminal gC1q domain; it functions to regulate pro-TGF-beta processing and to favor a correct functioning of lymphatic vessels. In a 2006 Cell study, it was discovered that EMILIN1 inhibits TGF-beta signaling by binding specifically to the proTGF-beta precursor and preventing its maturation by furin convertases in the extracellular space. In support of these findings, genetic inactivation of Emilin1 causes increased TGF-beta signaling in the vascular wall. In a MCB 2008 study it was discovered that Lymphatic vascular morphological alterations in KO mice are accompanied by functional defects, such as mild lymphedema, a highly significant drop in lymph drainage, and enhanced lymph leakage.
Antibody Overview 1. Clone name: HE1-1H2/G8 2. Specificity: human EMILIN1 3. Species/Isotype: Murine (IgG2a/k isotype) 4. This antibody can be used for the following applications and corresponding conditions: _X_ ELISA: capture ____ detection __x__ --X-- immunohistochemistry on frozen tissues _X_ Western Blot _X_ function blocking 5. What is the best positive tissue/cell line to use in testing this antibody? In vivo: aorta; in vitro: smooth muscle cells and fibroblasts. 6. Related Publications: - Colombatti A et al. J. Cell Biol. 100, 18-26, 1985 - Bressan GM et al. J. Cell Biol. 121, 201-212, 1993 - Doliana R et al. J. Biol. Chem. 274, 16773-16781, 1999 - Zanetti M. et al. Mol. Cell Biol. 24, 638-650, 2004 - Zacchigna L.et al. Cell 124, 929-942, 2006 - Danussi C. et al., Mol. Cell Biol. 28, 4026-4039, 2008 - Danussi C. et al. J Cell Biol 195:131-145
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More Information Alfonso Colombatti, MD Division Director, Experimental Oncology 2 Department of Molecular Oncology and Translational Medicine (DOMERT) acolombatti@cro.it +39-0434-659-365
Stomach stained with HE1-1H2/G8 (40x)