AssessmentoftheTickGutMetabolomeComposition
Thegutmicrobiomeisinvolvedintheregulationofvariousmetabolicpathwaysinthehost Somemetabolitesthataregeneratedandreleasedbythegutmicrobiotaplayavitalroleinhost metabolism,signaling,andimmunityCreativeProteomicsprovidesuntargetedmetabolomics servicetohelpyouanalyzethecompositionofintestinalmicrobialmetabolites
MetabolomicProfiling
UntargetedMetabolomics
TheLymediseaseagent,borreliaburgdorferi,colonizingthegutofthetickIxodesscapularis, cantransmitpathogenstovertebratehostsincludinghumansBburgdorfericolonization increasestheexpressionofseveraltickgutgenesincludingpixr.AbrogationofPIXRfunctionin vivoaltersthegutmicrobiome,metabolomeandimmuneresponsesChangesinthegut microbialmembersislikelytoinfluencethemetabolomeofthetickgutduetodifferencesinthe metabolicfunctionsuniquetothespecificbacteriageneraChangesinthecompositionof intestinalmetabolitescanbeanalyzedbymeansofuntargetedmetabolomics
ASSAYOVERVIEW
ExtractintestinalmetabolitesTheanalysisiscarriedoutusingultraperformanceliquid chromatographyandquadrupoletime-of-flightmassspectrometry(UPLC-QTOF).
2-Chloro-L-phenylalanine(Sigma)isusedasaninternalstandardTherawdataoftheMSis processedandverified.DataobtainedfromtheLC-QTOFMSsystemarefilteredfordenoising
toremovecompounds.Thefiltereddataarestandardizedbypeakareanormalizationand processedwiththeSIMCA
DATAOVERVIEW
Atotalof59metaboliteswereidentifiedonPositivemode(73hitsintotal),and64metabolites wereidentifiedonNegativemode(85hitsintotal)Atleast38and39differentiallyrepresented metaboliteswereidentifiedunderpositiveandnegativemode,respectively,betweenthegutsof ticksfedonPIXR-immunizedorovalbumin-immunizedmiceandisshowngraphicallyby hierarchicalclusteringtoheatmap.
Features
Fullyautomatic,high-throughput,one-stopfull-spectrummetabolomicsservice
UHPLC-QTOF-MS(Agilent1290)andUHPLC+ABQTOF5600(ACQUITYUHPLC)canbe usedforanalysis.
Quickturnaroundtime
Applications
Developmentandvalidationofmetabolite-relateddiseasediagnosticmarkers
Mechanismofmetaboliterelatedsignalingpathwaystudy.
Reference:
NarasimhanS,SchuijtTJ,AbrahamNM,etalModulationofthetickgutmilieubyasecreted tickproteinfavorsBorreliaburgdorfericolonizationNaturecommunications,2017,8(1):184