Nucleoprotein extraction protocol

Nucleoprotein Extraction Protocol Nucleoproteins are a class of binding proteins that structurally associated with nucleic acids, and widely exist in the nucleus of various biologic species. According to the type of nucleic acids, nucleoproteins can be divided into deoxyribonucleoproteins (DNA) and ribonucleoproteins (RNA). Studies focused on nucleoproteins are important for the understanding of disease-related genes and search of new proteomic markers for diseases. And the extraction of nucleoprotein is essential procedure for the analysis of specific spatial structure and biological functions. Here, we provide a protocol for nucleoprotein extraction from cultured cells and tissue in an efficient manner.

Reagents: PBS: pH 7.4, 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4.

Buffer A: pH 7.9, 10 mM HEPES, 1.5 mM MgCI2, 10 mM KCI, 1 mM DTT, with 1× protease inhibitor cocktail and phosphatase inhibitor cocktail.

Buffer B: pH 7.9, 20 mM HEPES, 1.5 mM MgCI2, 25% glycerol, 420 mM NaCI, 0.2 mM EDTA, 1 mM DTT, with 1× protease inhibitor cocktail and phosphatase inhibitor cocktail.

Buffer C: pH 7.9, 20 mM HEPES, 20% glycerol, 100 mM KCI, 0.2 mM EDTA, 1 mM DTT, with 1× protease inhibitor cocktail and phosphatase inhibitor cocktail. https://www.creative-diagnostics.com/nucleoprotein-extraction-protocol.htm