X-ray imaging has unique capabilities to reveal hidden defects buried within a material or device. From finding unwanted contaminants in rechargeable batteries to quality control of electrical connections in advanced computer chips, images obtained with X-rays provide important and actionable insights. But what are the relevant factors required to produce an X-ray image? Here we will outline the main concepts within the “imaging chain” and describe how they contribute to produce an X-ray image, with particular emphasis on micro and nano scale imaging. In addition, we will clarify some key concepts surrounding resolution.