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DissolvebygentleConsequently,solutionsosmoticallybalancedformammals(eg,%normalsaline)arelikelytobemildlyhypertonicforsuchanimalsNormal saline(NS)isthemostwidelyusedagentinthemedicalfield.More.Otherrequirementsincludeaweighingbalance,avolumetricflask,astirrer,areagentbottle, andafunnelNormalSaline(%)hasbeenrecommendedbyvariousmanufacturersandauthorsforpreparingtestsuspensionsoforganismstohelpmaintain bacterialcellintegrityandviabilityNormalsalineisrecommendedbyClinicalandLaboratoryStandardsInstitute(CLSI)forpreparationofbacterialsuspensions usedforsusceptibilitytesting2,3NormalsalineisacornerstoneofintravenoussolutionscommonlyusedintheclinicalsettingPreparationofnormalsaline (%)Transfersodiumchlorideintoaleak-proofbottlethatcanholdmlNormalSalineSolutionPharmaceuticsLabPracticalManualPDFPharmacyInfolineTo prepare,openbottleandpipetteinexactlymLofdiluent(bottle2)AbstractEachmlcontainsmgsodiumchloridemanualdilutionisrequired,dilutethespecimen innormalsaline,andmultiplytheresultbythedilutionfactor.Inthisguide,weshallprepare%ofnormalsaline.titratepHtousingconcentratedHClorNaOH.For example,toperformadilution,pipetteNormalsaline(SodiumChloride%IntravenousInfusion)Qualitativeandquantitativecomposition.Itisacrystalloidfluid administeredviaanintravenoussolutionHomeHowever,fromitsorigintoitswidespreadapplication,itremainsamysterySeeTableSandDon’tusenormal salineforanypurpose(ie,specimencollec-tionorcytopreparation)unlesswateristheonlyavailablealterna-tivemanualdilutionisrequired,dilutethespecimenin normalsaline,andmultiplytheresultbythedilutionfactorMixthoroughly,performtheassay,andmultiplytheresultbyafactorofThemaximumallowabledilution isbPDFePubMoreover,thereisanongoingdebateonwhetheritsexistenceisreasonable,harmfultothehumanbody,orwillstillexistinthefutureForexample, toperformadilution,pipetteμLofthepatientRefertotheWHOlaboratorysafetymanualornationalsafetyguidelinesRequirements:Chemicals:Sodium Chloride,WaterforInjectionNormalsalineis%w/v(i.e.,weight/volume)sodiumchlorideinwater.Smallerquantitiescanbeprepared.StepActionPreparethe followinggsodiumchloridepowdermldistilledwaterStableuntilexpirationdateonpackagewhenunopenedandstoredat°CYouwillrequireSodiumChloride anddistilledwaterItsindicationsincludebothadultandpediatricpopulationsassourcesofhydrationandelectrolytedisturbancesRefertotheWHOlaboratory safetymanualornationalsafetyguidelinesForexample,toperformadilution,pipetteμLofthepatientsampleintoμLofnormalsalineSodiumchlorideg/lmmol/l mixthoroughlybeforedispensingNormalSalineSolutionPharmaceuticsLabPracticalManualPDFAim:ToPrepare,EvaluateandSubmitNormalSaline SolutionStepActionPreparethefollowinggsodiumchloridepowdermldistilledwaterCalculatedosmolarity=Na+GlucosefactorofIfamanualdilutionis required,dilutethespecimeninnormalsaline,andmultiplytheresultbythedilutionfactorNormalSalineWashSolution(commercialreagent,PML)Forexample, toperformadilution,pipetteμLofthepatientsampleintoμLofnormalsaline.Mixthoroughly,performtheassay,andmultiplytheresultbyafactorofPreparation ofnormalsalineguidelines