Botupharma USA Product Catalog 2023

PRODUCT GUIDE

EQUINE LIVESTOCK & CANINE

04

05

04

05

BOTUPHARMA USA

ANIMAL BIOTECHNOLOGY Located on five continents providing Assisted Reproduction Products and Performance Supplements for large and small animals, Botupharma® has become an industry reference, not only because its products are based in science but, indeed, being the science behind its products.

The wide range of high-quality products is due to continuous research, multidisciplinary team effort and by internationally renowned professors and scientists’ assistance.

For more information

Botupharma® has an infrastructure that complies with the production standards in Brazil and the United States and rigorous field-testing, to create innovative products, reaching results that matter.

www.botupharmausa.com

04

05

SUMMARY

BOVINE SEMEN EXTENDER

SUPPLEMENTS

Botubov®

PERFORMANCE Pre-Racing Mega Power Creatine Speed

04 04 05

SUPPORT Revitalize Red Up® Entrin Horse

06 07 07

20

CANINE SEMEN EXTENDER Botudog® Botudog Turbo Botudog Freezing

22 22 22

GROWTH AND STRENGTHENING Newborn

08

REPRODUCTION Broodmare Broodmare Plus Stallion Plus

09 09 10

REPRODUCTION EQUINE SEMEN EXTENDERS Botusêmen® Botuturbo Extender Test Kit® Botusêmen Special Botusêmen Gold Botucrio®

13 13 13 14 14 15

LABORATORY Sperm Filter Red Cushion Equipuretm Botuembryo® Botuvital

17 17 17 17 17

ACCESSORIES Vagina Artificial Kit Botuflex® Botuia

04

18 18 18

05

SUPPLEMENTS NO DOPING HIGH BIOAVAILABILITY HIGH QUALITY INGREDIENTS DESIGNED BY VETERINARIANS APPROVED BY HORSES

Performance 04

05

SUPPLEMENTS

PRE-RACING NO DOPING

BEAT THE LEADER

MEGA POWER CREATINE NO DOPING

Combining stimulating ingredients, carefully selected to ensure horses’ best performance.

PRE-RACING is considered the most complete nutritional booster on the market, offering in its formulation everything essential to competition: Strength, energy, endurance, and faster muscular recovery.

This novel formulation associates substances that aid the animal in achieving peak performance when needed, such as Creatine with HMB (beta-hydroxy-beta-methyl butyrate) and Alanine. The presence of Chromium Picolinate also enhances work performance.

HMB: Burst of energy and strength for high-intensity work.

CREATINE: Energy for better muscle activity.

GAMMA-ORYZANOL: Increases the metabolism of fat and synthesis of protein. Improves and supports muscle development and strength, in addition to natural antioxidant properties.

Indication for use Supplementation of vitamins, minerals, and amino acids designed for all breeds of athletic horses submitted to training and competition in the most diverse equestrian modalities.

Feeding directions Supply it orally. For training animals, provide a dose of 19 g/animal (1/2 syringe) daily. For animals in competition, provide a dose of 38 g/animal (1 syringe) before the race.

Ingredients Sorbitol, L-Creatine, L-Arginine, Maltodextrin, L-Carnitine, Beta-Hydroxy-Beta-Methyl Butyrate, Rice Bran, Caramel Flavor, Betaine, L-Glutamine, Taurine, Silicon Dioxide, L-Leucine, L-Alanine, L-Valine, L-Isoleucine, Vitamin C, Caramel Dye, Chromium Picolinate, B.H.T. (Preservative), and Vitamin E. GUARANTEED ANALYSIS Maltodextrin (min) Creatine (min) L-Arginine (min) L-Carnitine (min) Beta-Hydroxy-Beta-Methyl Butyrate (min) Betaine (min) L-Glutamine (min) Taurine (min) L-Leucine (min) L-Alanine (min) L-Isoleucine (min) L-Valine (min) Chromium (min) Vitamin E (min) Vitamin C (min) Gamma-Oryzanol (min)

Presentation Syringe containing 1.3oz (38 g).

04

5 16 8.2 4.2 3.8 2.5 2.2 2 1.5 0.75 0.75 0.75 130 4,082.32 1,814.36 453.59

% % % % % % % % % % % % ppm UI/lb mg/lb mg/lb

SO MUCH MORE THAN CREATINE 62-days supply

Optimize energy production and improve muscle performance.

MEGA POWER CREATINE contains high concentrations of Monohydrated Creatine, the most effectively absorbed form by horses. In addition, the formulation contains prebiotics that improve intestinal microbiota and consequently enhance nutrient absorption.

And, Gamma-Oryzanol to potentialize the muscle mass gain.

Indicated for athletic animals and auction-preparing horses.

CREATINE: Optimizes the formation of muscle mass. Increases muscle strength and energy, enhancing the horse’s performance in high-intensity exercise.

MOS (Mannanoligosaccharides) and FOS (Fructo-oligosaccharides): Prebiotics that support optimal nutrition, improve intestinal microbiota, and increase exercise performance.

L-GLUTAMINE: Increases energy supply, reduces muscle inflammation, and prevents post-exercise fatigue.

Indication for Use Food supplement containing creatine, L-glutamine, and prebiotics is indicated for horses during intense training or competitions.

Feeding Directions Add the supplement powder to the feed.

Initial dose: Administer 6 scoops (120 g) daily for the first 14 days.

Maintenance dose: Administer 2 scoops (40 g) daily. ingredients Creatine Monohydrate, Dextrose, L-Glutamine, Yeast Cell Wall, Inactive Brewers Yeast, Rice Bran, Fructooligosaccharides, and Artificial Flavour.

GUARANTEED ANALYSIS Creatine Monohydrate (min) Dextrose (min) L-Glutamine (min) Beta Glucan (min) Fructooligosaccharides (FOS) (min) Gamma Oryzanol (min) Inactivated Brewers Yeast (min) Mannan Oligosaccharides (MOS) (min)

Presentation Powder: Net Weight 5.5 lb (2.5 kg).

80.00 6.20 3.50 680.380 453.590 680.390 4535.920 7257.470

% % % mg/lb mg/lb mg/lb mg/lb mg/lb

NO DOPING

RECORD ON THE TRACK

Critical ingredients for muscle development, providing strength and energy for peak performance.

The most complete Performance Supplement available on the market! It combines the essential daily requirements of vitamins, minerals, and amino acids, as well as contributing to the nutritional demands of high-performance horses.

SPEED formulation is enriched with antioxidants and offers a special association of Dimethylglycine, which acts as a buffer against the lactic acid release, with Gamma-Oryzanol, a natural anabolic agent extracted from rice bran.

GAMMA-ORYZANOL: Antioxidant with anabolic effects, stimulates testosterone production for greater muscle growth and development.

BCAA (branched-chain amino acid): Amino acids that optimize energy production and muscle development for better horse performance.

DIMELTHYGLYCINE: Potentializes the use of oxygen for energy production by reducing the release and accumulation of high levels of lactate in muscles.

L-LYSINE: Optimizes muscle mass gain and promotes strength to improve horse performance.

Indication for use SPEED supplement offers energy and metabolic support for hard-working, working and competition horses, ensuring maximum performance. Enriched with nano encapsulated Omega-3 and Omega-6.

Feeding directions For animals in hard-working mode, administer two rounded scoops (100 g). Give 2 scoops at one daily feeding or 1 scoop at two daily feedings.

For working animals, administer 1 rounded scoop (50 g). Give 1 scoop at one daily feeding.

GUARANTEED ANALYSIS Dextrose (min) Maltodextrin (min) Linolenic Acid (Omega-3) (min) Linoleic Acid (Omega-6) (min)

Percent 12.50 12.07 3.62 1.76

% % % %

Per 100 g 12,504 mg 12,066 mg 3,622 mg 1,758 mg

Amino Acids Aspartic Acid (min) Alanine (min) Arginine (min) Cysteine (min) Isoleucine (min) Glutamine (min) Leucine (min) Lysine (min) Glycine (min) Phenylalanine (min) Histidine (min) Methionine (min) Proline (min) Tyrosine (min) Threonine (min) Valine (min)

Percent 1.82 0.91 1.98 0.36 0.90 4.79 1.38 3.91 1.20 0.90 0.45 0.48 0.66 0.60 0.90 1.26

% % % % % % % % % % % % % % % %

Per 100 g 1,817 mg 908 mg 1,977 mg 360 mg 900 mg 4,794 mg 1,378 mg 3,914 mg 1,198 mg 900 mg 450 mg 480 mg 660 mg 600 mg 900 mg 1,258 mg

Trace Minerals Chromium (min) Cobalt (min) Copper (min) Iron (min) Manganese (min) Selenium (min) Zinc (min)

PPM 3.2 7.88 119 2,400 1,378 9.6 1,260

ppm ppm ppm ppm ppm ppm ppm

Per 100 g 0.3 mg 0.8 mg 12 mg 240 mg 138 mg 1 mg 126 mg

Vitamins Biotin (min) Folic Acid (min) Vitamin B12 (min) Vitamin B6 (min) Vitamin E (min)

Per pound 13 mg 273 mg 0.2 mg 140 mg 2,700 UI

Per 100 g 2.8 mg 60 mg 0.04 mg 30 mg 600 UI

Other Additives Beta Carotene (min) Carnitine (min) Dimethylglycine (min) Gamma Oryzanol (min) Methylsulfonylmethane (min)

Per Pound 1,359 mg 140 mg 8,156 mg 4,122 mg 32,000 mg

Per 100 g 300 mg 30 mg 1,800 mg 909 mg 7,060 mg

SUPPLEMENTS

SPEED

For basic composition and guaranteed levels of Speed liquid presentation, access the QR CODE:

Ingredients Alpha-Linolenic Acid, Dextrose, Maltodextrin, Linolenic Acid, Methylsulfonylmethane, L-Glutamine, L-Lysine, Potassium Chloride, Monobasic Calcium Phosphate, Magnesium Amino Acid Chelate, L-Arginine, Aspartic Acid, Dimethylglycine, L-Leucine, L-Valine, Iron Amino Acid Chelate, Vitamin E (alpha tocopherol acetate), L-Glycine, Manganese Amino Acid Chelate, L-Alanine, Gamma Oryzanol, L-Phenylalanine, L-Isoleucine, L-Threonine, Phosphorus Amino Acid Chelate, L-Proline, Zinc Amino Acid Chelate, L-Tyrosine, L-Methionine, L-Histidine, L-Cysteine, Beta Carotene, Selenium Amino Acid Chelate, Cobalt Amino Acid Chelate, Cooper Amino Acid Chelate, Artificial Flavour, Folic Acid, Vitamin B12, L-Carnitine, Vitamin B6 (pyridoxine hydrochloride), Biotin, and Chromium Tripicolinate.

Presentation Powder: Net Weight 6.6 lb (3 kg).

Liquid: Net Contents 71 fl oz (2.2 qt) (2.1 L).

05

SUPPLEMENTS NO DOPING HIGH BIOAVAILABILITY HIGH QUALITY INGREDIENTS DESIGNED BY VETERINARIANS APPROVED BY HORSES

Support 06

SUPPLEMENTS

REVITALIZE NO DOPING

GUARANTEED ANALYSIS

ENERGY AND VIGOR

Replenishment of vital nutrients for speedy horse recovery REVITALIZE was designed to support animals' nutritional management in recovery and improve body condition and immunity. The formula provides better absorption of macro and microelements through beta-glucans and positively influences energy metabolism.

In addition, it is indicated for final third pregnant and nursing mares. It can also be helpful for foals under stressful situations, such as weaning, diet change and diarrhea.

VITAMIN C: antioxidant and immunostimulant, reduces oxidative stress and muscle fatigue and strengthens the immune system, ensuring support for the horse's recovery.

L-GLUTAMINE: Conditional essential amino acid, for sick horses or horses with comorbidities, regulates the immune system and improves the health of the gastrointestinal tract.

MALTODEXTRIN: Carbohydrates with a high glycemic index ensure muscle cell replenishment with glycogen and maintenance of metabolic functions.

Indication for use REVITALIZE PLUS supplement offers energy support for body wellness. It contains high concentrations of maltodextrin for slow-release energy, combined with maximum energy conversion

by B vitamins and choline. REVITALIZE is enriched with a blend of bio-available macro and micronutrients, including nano encapsulated Omega 3.

Feeding directions Supply orally. Administer 1 scoop (30 g) daily Ingredients Dextrose, Maltodextrin, Yeast Cell Wall, Alpedha-Linolenic Acid, Vitamin E (alpha tocopherol acetate), L-Lysine, Magnesium, L-Alanine, Choline Chloride, L-Glutamine, L-Methionine, Linoleic Acid, Methylsulfonylmethane, Mannan Oligosaccharides, Zinc Amino Acid Chelate, L-Threonine, Manganese Amino Acid Chelate, Glutamic Acid, L-Arginine, Iron Amino Acid Chelate, L-Valine, Vitamin C (ascorbic acid), Fructooligosaccharides, L-Histidine, Selenium Amino Acid Chelate, Folic Acid, Cooper Amino Acid Chelate, Vitamin B12, Calcium Pantothenate, Vitamin A, Artificial Flavor, Vitamin B1, Niacin, Vitamin B6 (pyridoxine hydrochloride), Vitamin K3, Vitamin B2, Vitamin D3, and Biotin.

Dextrose (min) Maltodextrin (min) Linolenic Acid (Omega-3) (min) Linoleic Acid (Omega-6) (min)

Percent 16.43 16.43 1.11 0.51

% % % %

Per 100 g 4,930 mg 4,930 mg 332 mg 152 mg

Amino Acids Alanine (min) Arginine (min) Glutamic Acid (min) Glutamine (min) Histidine (min) Lysine (min) Methionine (min) Threonine (min) Valine (min)

Percent 3.78 1.48 1.48 2.99 0.33 5.75 2.46 1.56 0.99

% % % % % % % % %

Per 100 g 1,134 mg 444 mg 444 mg 897 mg 99 mg 1,725 mg 739 mg 468 mg 296 mg

Macrominerals Magnesium (min) Sulfur (min)

Percent 1.48 % 0.72 %

Per 30 g 444 mg 217 mg

Trace Minerals Cooper (min) Iron (min) Manganese (min) Selenium (min) Zinc (min)

PPM 296 2,629 2,607 23 4,141

ppm ppm ppm ppm ppm

Per 30 g 9 mg 79 mg 78 mg 0.7 mg 124 mg

Vitamins Biotin (min) Choline (min) Folic Acid (min) Pantothenic Acid (min) Vitamin A (min) Vitamin B1 (min) Vitamin B12 (min) Vitamin B2 (min) Vitamin B3 (min) Vitamin B6 (min) Vitamin C (min) Vitamin D3 (min) Vitamin E (min) Vitamin K3 (min)

Per Pound 36 mg 8,944 mg 894 mg 716 mg 357,758 UI 447 mg 1 mg 149 mg 373 mg 298 mg 3,265 mg 39,503 UI 13,416 UI 179 mg

Per 30 g 2 mg 592 mg 59 mg 47 mg 23,662 UI 30 mg 0.05 mg 10 mg 25 mg 20 mg 216 mg 2,613 UI 887 UI 12 mg

Other additives Per Pound Beta glucan (min) 29,828 mg Fructooligosaccharides (FOS) (min) 0.39 % Mannan Oligosaccharides (MOS) (min)2.14 % Methylsulfonylmethane (min) 9,938 mg

Per 30 g 1,973 mg 118 mg 641 mg 657 mg

For basic composition and guaranteed levels of Speed liquid presentation, access the QR CODE:

Presentation Powder: Net Weight 21.16 oz (1.3 lb) (600 g).

Liquid: Net Contents 51 fl oz (1.6 qt) (1.5L).

07

SUPPLEMENTS

RED UP® NO DOPING

EXTRA STRENGTH

Ingredients Water, Linseed Oil, Iron Amino Acid Complex, Folic Acid, L-Tyrosine, Zinc Amino Acid Chelate, Histidine, Ascorbic Acid, Cobalt Amino Acid Chelate, Copper Amino Acid Chelate, Pantothenic Acid, Vitamin K3, Pyridoxine Hydrochloride, Thiamine Hydrochloride, Niacin, B.H.T. (Preservative), Riboflavin, Methylparaben (Preservative), Propylparaben (Preservative), and Cyanocobalamin.

Indication for use Supplement composed of vitamins, minerals, and amino acids, indicated for horses, small ruminants, and pigs.

Supplement to stimulate the production of red blood cells and restore the animal's energy and vitality after intensive training RED UP is enriched with B complex vitamins and iron, supporting red blood cell production.

IRON: Micromineral constituent of hemoglobin, essential for oxygen transport by red blood cells.

FOLIC ACID: Important for stimulating hematopoiesis, ensuring that it occurs correctly.

VITAMIN B12: Like folic acid, supports red blood cells production.

Presentation Liquid: Net Contents 51 fl oz (1.6 qt) (1.5L).

Feeding directions Supply daily orally.

Adult horses: 20 mL per day.

Foals and ponies: 15 mL daily.

Newborn foals: 10 mL daily.

GUARANTEED ANALYSIS Tyrosine (min) Histidine (min) Omega-3 (min) Omega-6 (min) Iron (min) Zinc (min) Copper (min) Cobalt (min) Vitamin C (min) Vitamin B6 (min) Vitamin B1 (min) Vitamin K3 (min) Vitamin B2 (min) Vitamin B12 (min) Niacin (min) Pantothenic Acid (min) Folic Acid (min)

3.1 2.3 10.4 3.6 15,000 5,100 650 100 5 453 408 272 136 5,443 340 635 18

% % % % ppm ppm ppm ppm g/lb mg/lb mg/lb mg/lb mg/lb mcg/lb mg/lb mg/lb mg/lb

ENTRIN HORSE NO DOPING

GOOD FOR THE GUT

The ideal prebiotic support for equine gastrointestinal health The formulation combines prebiotics, toxin adsorbents, and glutamine to improve intestinal immunity and promote enterocyte recovery.

L-GLUTAMINE: Effective amino acid for maintaining intestinal health in horses.

FOS and MOS: Prebiotics that stimulate the development and proper functioning of the intestinal microbiota.

CALCIUM and SODIUM ALUMINOSILICATE: Mineral salts with toxin adsorbent action essential to improve stool consistency.

Feeding directions Supply daily orally.

Initial dose: for adult horses administer 1 tube every 12 hours, for foals administer 1/2 tube every 12 hours.

Maintenance dose: for adult horses administer 1 tube once daily, for foals administer 1/2 tube once daily.

08

Ingredients Rice Bran Oil, Hydrated Sodium Calcium Aluminosilicate, L-Glutamine, Kaolin, Yeast Cell Wall, Magnesium Amino Acid Chelate, Threonine, L-Alanine, Iron Amino Acid Chelate, Zinc Amino Acid Complex, Glutamic Acid, Ascorbic Acid, Fructo-Oligosaccharides, Selenium Amino Acid Complex, Copper Amino Acid Chelate, Cobalt Amino Acid Chelate, Vitamin A, Magnesium Phosphate, Manganese Amino Acid Complex, Alpha-Tocopherol Acetate (Vitamin E), and Chromium Amino Acid Chelate.

Indication for use Supplement composed of vitamins, minerals, and amino acids for horses.

GUARANTEED ANALYSIS Hydrated Sodium Calcium Aluminosilicate (min) L-Glutamine (min) L-Alanine (min) Threonine (min) Glutamic Acid (min) Phosphorus (min) Magnesium (min) Iron (min) Zinc (min) Copper (min) Selenium (min) Cobalt (min) Manganese (min) Chrome (min) Vitamin A (min) Vitamin E (min) Vitamin C (min) Beta Glucans (min) Mannan oligosaccharides (min) Fructo-oligosaccharides (min)

Presentation Syringe containing 1.5 oz (43 g).

16.27 % 11.62 % 2.09 % 2.09 % 1.04 % 0.00186 % 6,976 ppm 3,023.2 ppm 2,930.2 ppm 302.3 ppm 22.7 ppm 13.9 ppm 3.4 ppm 0.006 ppm 254,011 UI/lb 9,071 UI/lb 2,310 mg/lb 10.52 g/lb 5.26 g/lb 1,265.7 mg/lb

SUPPLEMENTS NO DOPING HIGH BIOAVAILABILITY HIGH QUALITY INGREDIENTS DESIGNED BY VETERINARIANS APPROVED BY HORSES

Growth and strengthening 09

SUPPLEMENTS

NEWBORN NO DOPING

THE FIRST STEP TOWARDS A FUTURE CHAMPION TITLE

Combines ingredients for optimal immune performance, critical for foal growth.

NEWBORN is an oral paste supplement that offers ideal nutritional support for the newborn foal. It contains a potent adsorbent that removes the toxins produced by infectious agents, preventing gastrointestinal disorders.

L-GLUTAMINE: Amino acid reduces inflammatory responses, stimulating immunoregulation, and provides intestinal protection.

VITAMIN A: Acts as an antioxidant, ensuring cellular protection against the action of free radicals.

FOLIC ACID: Acts directly on regulating cell synthesis, which is crucial to ensure quality growth.

Indication for use Supplement based on vitamins, chelated minerals, amino acids, antioxidants, and lipids, indicated for newborns and nursing foals.

Feeding directions Supply It orally.

For newborns: administer one syringe orally after 6 hours of first nursing, and repeat it after 24 hours.

For debilitated animals, administer one syringe every 24 hours for 7 days.

Ingredients Linseed Oil, Hydrated Sodium Calcium Aluminosilicate, L-Alanine, L-Arginine, Potassium Chloride, Glycine, L-Leucine, L-Phenylalanine, L-Valine, L-Isoleucine, L-Aspartic Acid, L-Threonine, L-Proline, Magnesium Amino Acid Chelate, L-Tyrosine, Monocalcium Phosphate, Iron Amino Acid Chelate, L-Cysteine, L-Lysine Monohydrochloride, L-Methionine, Zinc Amino Acid Chelate, Copper Amino Acid Complex, Manganese Amino Acid Chelate, Pyridoxine Hydrochloride, Riboflavin, Folic Acid, Vitamin A, Biotin, Vitamin D3, Alpha-Tocopherol Acetate, Cobalt Amino Acid Chelate, Selenium Yeast, Cyanocobalamin, and B.H.T. (Preservative).

Presentation Syringe containing 1.5 oz (43 g).

10

NÍVEIS DE GARANTIA POR KG Aspartic Acid (min) L-Arginine (min) L-Cysteine (min) Phenylalanine (min) Glycine (min) Isoleucine (min) L-Threonine (min) L-Alanine (min) L-Lysine (min) L-Leucine (min) L-Methionine (min) Proline (min) Tyrosine (min) L-Valine (min) Omega-3 (min) Omega-6 (min) Calcium (min) Calcium (max) Phosphorus (min) Potassium (min) Magnesium (min) Fluorine (max) Iron (min) Zinc (min) Copper (min) Manganese (min) Selenium (min) Cobalt (min) Vitamin A (min) Vitamin E (min) Vitamin D3 (min) Vitamin B12 (min) Vitamin B2 (min) Vitamin B6 (min) Folic Acid (min) Biotin (min)

0.60 1.50 0.20 0.80 0.90 0.70 0.60 1.60 0.19 0.90 0.13 0.45 0.40 0.80 6.00 0.20 0.05 0.08 0.23 1.00 0.41 0.00015 2,000 200 100 100 2 2 8,626 908 1,816 0.15 37.68 44.49 9.08 8.17

% % % % % % % % % % % % % % % % % % % % % % ppm ppm ppm ppm ppm ppm IU/lb IU/lb IU/lb mg/lb mg/lb mg/lb mg/lb mg/lb

Reproduction 11

BROODMARE REPRODUCTION

NO DOPING

BROODMARE PLUS NO DOPING

THE BEST FOR YOUR BREEDER AND EMBRYO DONORS MARES IN REPRODUCTIVE PROGRAMS Provides high reproductive rates for breeder mares and embryo donors.

BROODMARE supplement supports optimum fertility, ensuring high performance and pregnancy rates during the breeding season. Enriched with L-Arginine, L-Carnitine and Omega-3 for maximum reproductive efficacy.

Ideal for optimizing the fertility of mares in ICSI programs or with advanced age.

ARGININE: Essential amino acid that promotes increased blood perfusion in the ovaries and uterus.

BROODMARE PLUS improves the oocyte’s metabolic function and the embryonic development of supplemented mares, consequently increasing fertility rates.

OMEGA 3[1][2]: A polyunsaturated fatty acid that acts on the health of the endometrium and improves the quality of embryos produced.

High concentrations of:

CHROMIUM: Mineral with action on glucose metabolism and protein synthesis.

Indication for use Liquid supplement enriched with vitamins, minerals, amino acids and fatty acids to support and maintain optimum fertility.

Feeding directions Supply daily orally.

Administer 50 mL once daily or 25 mL twice daily.

Ingredients Water, Linseed Oil, L-Arginine, Zinc Amino Acid Chelate, Folic Acid, Vitamin B6 (pyridoxine hydrochloride), Cooper Amino Acid Chelate, Beta Carotene (source of Vitamin A), Selenium Amino Acid Chelate, Vitamin E (alpha tocopherol acetate), Artificial Green Apple Flavor and Vitamin B12.

GUARANTEED ANALYSIS L-Arginine (min) Linolenic Acid (Omega-3) (min) Linoleic Acid (Omega-6) (min) Copper (min) Selenium (min) Zinc (min) Vitamin A (min) Vitamin E (min) Vitamin B12 (min) Vitamin B6 (min) Folic Acid (min)

4,0 10,0 5,0 520 40 3,000 103,030 5,448 0.4 272.4 544.8

% % % ppm ppm ppm IU/lb IU/lb mg/lb mg/lb mg/lb

Presentation Liquid: Net content 51 fl oz (1.6 qt) (1.5 L)

CARNITINE: An amino acid with antioxidant action that protects oocytes and embryos during in vitro production.

ARGININE[1][2][3]: Essential amino acid that promotes increased blood perfusion in the ovaries and uterus.

OMEGA 3[1][2]: A polyunsaturated fatty acid that acts on the health of the endometrium and improves the quality of the embryos produced.

Indication for use Supplement composed of vitamins, minerals, and amino acids, indicated for donors in ICSI programs and older mares.

Feeding directions Supply daily orally.

Administer 50 mL once daily or 25 mL twice daily.

Ingredients Water, Linseed Oil, L-Carnitine, L-Arginine, Ascorbic Acid (Vitamin C), Zinc Amino Acid Chelate, Alpha-Tocopherol Acetate (Vitamin E), Selenium Yeast, Copper Amino Acid Chelate, Artificial Green Apple Flavor, Beta Carotene (Source of Vitamin A), Folic Acid, Pyridoxine Hydrochloride, and Cyanocobalamin.

GUARANTEED ANALYSIS L-Carnitine (min) L-Arginine (min) Omega-3 (min) Omega-6 (min) Copper (min) Selenium (min) Zinc (min) Vitamin A (min) Vitamin E (min) Vitamin B12 (min) Vitamin B6 (min) Vitamin C (min) Folic Acid (min)

30 % 10 % 15 % 5 % 520 ppm 40 ppm 3,000 ppm 103,030 IU/lb 5,448 UI/lb 0.4 mg/lb 272.1 mg/lb 18 g/lb 544.3 mg/lb

Presentation Liquid: Net Contents 51 fl oz (1.6 qt) (1.5 L).

12

NO DOPING

REPRODUCTION

STALLION PLUS

MAXIMUM REPRODUCTIVE EFFICIENCY

STALLION PLUS is an innovative supplement with high nutrient concentrations, prompting high sperm production and maximum reproductive efficiency.

STALLION PLUS supplement supports optimum stallion fertility to ensure high performance during the breeding season.

L-CARNITINE[1][2]: Amino acid with antioxidant action and promoter of spermatozoid energy metabolism.

OMEGA 3[1][2]: Polyunsaturated fatty acid that makes up the spermatozoid membrane and protects sperm cells against low temperatures.

Indication for use STALLION PLUS is a liquid supplement enriched with vitamins, minerals, amino acids and fatty acids to support and maintain optimum fertility.

Feeding directions Supply it orally.

Administer 50 mL daily. Give 50 mL at one daily feeding or 25 mL at two daily feedings.

Ingredients Water, Linseed Oil, L-Carnitine, L-Arginine[1][2], Folic Acid, Zinc Amino Acid Chelate, L-Glycine, Vitamin B6 (pyridoxine hydrochloride), Beta Carotene (source of vitamin A), Copper Amino Acid Chelate, Selenium Amino Acid Chelate, Vitamin E (alpha tocopherol acetate), Artificial Green Apple Flavor, and Vitamin B12.

GUARANTEED ANALYSIS L-Carnitine (min) L-Arginine (min) Glycine (min) Linolenic Acid (Omega-3) (min) Linoleic Acid (Omega-6) (min) Copper (min) Selenium (min) Zinc (min) Vitamin A (min) Vitamin E (min) Vitamin B12 (min) Vitamin B6 (min) Folic Acid (min) L-Carnitine (min) L-Arginine (min) Glycine (min)

30.0 % 2.0 % 0.1 % 10 % 5 % 60 ppm 60 ppm 1,200 ppm 103,030 IU/lb 9,070 IU/lb 0.09 mg/lb 272 mg/lb 544 mg/lb 30.0 % 2.0 % 0.1 %

Presentation Liquid: Net Contents 51 fl oz (1.6 qt) (1.5 L).

13

14

Reproduction Equine Semen Extenders

15

REPRODUCTION

BOTUSEMEN®

BOTUTURBO®

EQUINE SEMEN EXTENDER FOR SAFE SPERM TRANSPORT

IMPROVE THE SPERM MOTILITY

BotuSemen[1][2][3] is an extender developed after years of research, and its current formulation enables the safe transport of spermatozoa until the time of insemination or during the centrifugation process for freezing protocols. Suitable for most stallions.

BotuTurbo[1][2] is an extender developed to improve the vigor of slow-moving sperm. It is highly recommended for semen transport and artificial insemination for samples that require better sperm motility.

How to prepare Add the sachet’s content to the sterile water and shake well.

How to prepare Add the sachet’s content to the sterile water and shake well.

How to use For cooled semen transport, the minimum dilution rate for preserving sperm motility during storage is 2 parts extender to 1 part semen, aiming for a final sperm concentration of 30 to 50 million total sperm per mL, as shown in the table below:

How to use For cooled transport, semen should be refrigerated at 5 OC. The minimum dilution rate for preserving sperm motility during storage is 2 parts extender to 1 part semen, aiming for a final sperm concentration of 30 to 50 million total sperm per mL, as shown in the table below:

SPERM CONCENTRATION

DILUTION (V:V)

< 60 million sperm/ mL 70 to 90 million sperm/ mL 100 to 200 million sperm/ mL 210 to 250 million sperm/ mL 260 to 300 million sperm/ mL

Centrifuge or filter with Sperm Filter 2:1 3:1 4:1 5:1

SPERM CONCENTRATION

DILUTION (V:V)

< 60 million sperm/ mL 70 to 90 million sperm/ mL 100 to 200 million sperm/ mL 210 to 250 million sperm/ mL 260 to 300 million sperm/ mL

Centrifuge or filter with Sperm Filter 2:1 3:1 4:1 5:1

Composition UHT sterilized skim milk powder, sugars, amino acids, antioxidants, and excipients.

For artificial insemination with fresh semen, dilute 1 part extender to 1 part semen (1:1; V:V). After semen dilution, wait 15 minutes to proceed with artificial insemination.

Storage and stability After mixing the contents of the sachet with sterile water, store it at 2 to 8oC for up to 3 days.

Composition UHT sterilized skim milk powder, sugars, amino acids, antioxidants, pentoxifylline and excipients.

Presentation Pack of 10 diluent bottles containing 100mL of sterile water and 10 sachets with 8.5g each.

One diluent bottle containing sterile water and 1 sachet with 17g.

Storage and stability After mixing the contents of the sachet with sterile water, store it at 2 to 8oC for up to 3 days.

QR CODE for More Information

Presentation Pack of 10 diluent bottles containing sterile water and 10 sachets with 8.5g each.

One diluent bottle containing sterie water and 1 sachet with 17g.

QR CODE for More Information

EXTENDER TEST KIT

FIND THE BEST EXTENDER FOR YOUR STALLION

16

Presentation Box containing 4 sachets and 4 bottles of purified water: 1 BotuSemen® 1 BotuSemen® Gold 1 BotuSemen® Special 1 BotuTurbo®

REPRODUCTION

BOTUSEMEN SPECIAL

MAXIMUM SPERM PROTECTION FOR BAD COOLER STALLIONS

BOTUSEMEN® GOLD

PROTECTION & HIGH FERTILITY BotuSemen Special[1] is an extender specially developed for stallions more susceptible to cold damage. Its composition is based on the addition of essential lipids to maintain the plasma membrane stability during the cooling process.

To improve your results, use BotuSemen Special during centrifugation in freezing protocols.

How to prepare Add the sachet’s content to the sterile water and shake well.

How to use The minimum dilution rate for preserving sperm motility during storage is 2 parts extender to 1 part semen, aiming for a final sperm concentration of 30 to 50 million total sperm per mL, as shown in the table below: SPERM CONCENTRATION

DILUTION (V:V)

< 90 million sperm/ mL

Centrifuge or filter with Sperm Filter and resuspend 100 million sperm/mL 2:1 2,5:1 3:1 3,5:1 4:1 4,5:1 5:1

90 to 110 million sperm/ mL 120 to 130 million sperm/ mL 140 to 160 million sperm/ mL 170 to 180 million sperm/ mL 190 to 210 million sperm/ mL 220 to 230 million sperm/ mL 240 to 250 million sperm/ mL

IMPORTANT: In cases where centrifugation before the cooling process is necessary, resuspend the sperm pellet at a final concentration of 100 million total sperm per mL. Composition UHT sterilized skim milk powder, sugars, amino acids, antioxidants, cholesterol and excipients.

Storage and stability After mixing the contents of the sachet with sterile water, store it at 2 to 8oC for up to 3 days.

Presentation Pack of 10 diluent bottles containing sterile water and 10 sachets with 8.5g each.

One diluent bottle containing sterile water and 1 sachet with 17g.

QR CODE for More Information

BotuSemen Gold[1] is an extender specially developed for stallions more susceptible to premature capacitation. The formulation contains casein, which may delay the capacitation process, improving pregnancy rates. In addition, BotuSemen Gold contains cholesterol, an essential component to protect the sperm membrane during the colling process.

To improve your results, use BotuSemen Special during centrifugation in freezing protocols.

How to prepare Add the sachet’s content to the sterile water and shake well.

How to use The minimum dilution rate for preserving sperm motility during storage is 2 parts extender to 1 part semen, aiming for a final sperm concentration of 30 to 50 million total sperm per mL, as shown in the table below: SPERM CONCENTRATION

DILUTION (V:V)

< 90 million sperm/ mL

Centrifuge or filter with Sperm Filter and resuspend 100 million sperm/mL 2:1 2,5:1 3:1 3,5:1 4:1 4,5:1 5:1

90 to 110 million sperm/ mL 120 to 130 million sperm/ mL 140 to 160 million sperm/ mL 170 to 180 million sperm/ mL 190 to 210 million sperm/ mL 220 to 230 million sperm/ mL 240 to 250 million sperm/ mL

IMPORTANT: In cases where centrifugation before the cooling process is necessary, resuspend the sperm pellet at a final concentration of 100 million total sperm per mL. Composition Casein, sugars, amino acids, antioxidants, cholesterol and excipients.

Storage and stability After mixing the contents of the sachet with sterile water, store it at 2 to 8oC for up to 3 days.

Presentation Pack of 10 diluent bottles containing sterile water and 10 sachets with 8.5g each.

One diluent bottle containing sterile water and 1 sachet with 17g.

QR CODE for More Information

17

REPRODUCTION

BOTUCRIO®

HIGHER PROTECTION & FERTILITY RATES FOR FROZEN SEMEN

BotuCrio is an innovative freezing extender developed for stallion sperm cryopreservation. The formula was designed after long years of research, fertility trials and field tests. The extender aims to protect the stallion sperm from cryodamage and maintain fertility post-thawing.

[1][2]

Scientific papers have demonstrated excellent results by using BotuCrio, since the extender provided higher motility parameters, preserved the sperm membrane integrity, and contributed to better fertility rates.

BotuCrio is the worldwide extender most used for stallion sperm cryopreservation.

DOWNLOAD THE BOTUPHARMA USA APP EASY TO PERFORM CALCULATIONS OF THE SEMEN CONCENTRATION Available for free on App Store and Google Play

BOTUPHARMA USA APP

18

How to use After centrifugation, discard the supernatant and add BotuCrio directly to the sperm pellet at a concentration of 200 million sperm per mL. Pack and seal the straws.

For the conventional freezing method, stabilize the straws at 5oC for 20 minutes.

Then, place the straws in nitrogen vapor (Styrofoam box), 3 to 6 cm above liquid nitrogen level for 20 minutes.

For thawing, the straws must be placed in a water bath at 37oC for 30 seconds or 46oC for 20 seconds.

Composition Sugars, antioxidants, amino acids, egg yolk, cryoprotectant and vehicle.

Storage and stability Store it frozen at -20ºC. After thawing, keep it refrigerated at 5ºC for up to 2 days.

Presentation Bottle containing 25 mL.

Laboratory 19

LABORATORY

SPERM FILTER

ALTERNATIVE TO CONCENTRATE SEMEN

SpermFilter[1][2] is a synthetic membrane filter with micropores allowing the passage of seminal plasma and bacteria, retaining only sperm cells.

As an innovation, the filter eliminates the need for a centrifuge and can be used routinely in cases where it would be used.

Furthermore, the filtration was demonstrated to be less traumatic and harmful to sperm when compared to conventional centrifugation, since the sperm pass passively through the filter.

How to use Dilute the semen with BotuSemen and place it in the SpermFilter. Gently swirl the diluted semen around the filter, touching the bottom of the filter to the Petri dish, until the extender and seminal plasma are completely removed. Wash the filter with an appropriate volume of either BotuSemen or BotuCrio extender.

SpermFilter can be reused 10 times for the same stallion.

RED CUSHION

PROTECTION & HIGHER RECOVERY IN SEMEN CENTRIFUGATION

RedCushion[1][2] is a high-density colloidal medium used in semen centrifugation to protect sperm cells, reducing damage caused by conventional centrifugation.

RedCushion-centrifugation semen produces less compacted pellets and a high sperm recovery rate. Due to its reddish color, it allows easy visualization of the pellet, as well as its removal.

How to use Dilute the semen with BotuSemen and place it in a centrifuge tube. Place 3 mL of RedCushion in the bottom of the centrifuge tube. Centrifuge at 1000g for 20 minutes. Using a pipette, remove the sperm pellet and resuspend sperm with an appropriate volume of either BotuSemen or BotuCrio.

BOTUVITAL

SOLUTION FOR SPERM EVALUATION

BotuVital[1][2] is a quick and practical alternative to assess sperm morphology and plasma membrane integrity.

It’s based on eosin-nigrosine staining, in which nigrosin increases the contrast between the background and sperm heads, making it easier to visualize, the eosin stains only dead sperm, turning them dark pink, while live sperm appear white.

20

DENSITY GRADIENT FOR SPERM SELECTION

WORK WITH THE BEST ONES EquiPure™[1][2] enhances equine sperm quality and viability by separating sperm by density gradient centrifugation. Sperm selection eliminates a high proportion of abnormal sperm, bacteria, defense cells, epithelial cells and cell debris, all sources of reactive oxygen species. This significantly increases sperm survival and its fertilizing potential. It can be used prior cooled transport, artificial insemination, sexing and freezing or after cooling and thawing.

How to use For more information, access the QR CODE below : Presentation Bottle containing 100 mL.

BOTUEMBRYO®

For more information, access the QR CODE below : Presentation 1 filter and 1 Petri dish.

EQUIPURETM

For more information, access the QR CODE below :

HOLDING AND TRANSPORT OF EMBRYOS

Presentation Bottles containing 20mL or 100 mL.

Solution for sperm evaluation based on Eosin-Nigrosin, allowing the analysis of sperm morphology and plasmatic membrane integrity, under light microscopy, with a 100x immersion objective.

How to use Make a smear with a mixture of 1 drop of fresh semen and 1 drop of BotuVital. Let smear air dry (do not dry on warming plate). Examine under oil in a light microscope (1000X).

For more information, access the QR CODE below : Presentation Bottle containing 10 mL.

BotuEmbryo is a medium for holding and transporting embryos at field conditions.

It has an innovative formulation based on scientific studies carried out by national and international researchers.

Its formulation is highly stable, allowing embryo transport at room temperature or refrigerated in suitable containers (BotuFlex) for up to 24 hours. The vial presentation does not require the use of syringes and needles.

Storage and stability Transport: room temperature for up to 3 days.

Storage: refrigerated at 2o to 8oC. Presentation Pack with 5 vials containing 8 mL.

LABORATORY

ARTIFICIAL VAGINA KIT

BOTUFLEX®

SAFE TRANSPORT OF SEMEN AND EMBRYOS

BETTER STALLION COMFORT DURING SEMEN COLLECTION

BotuFlex is a highly versatile and safe system for cooling semen designed to provide adequate cooling curves, both at 5ºC and 15ºC. Its sealing and density allow the shipped samples to reach their final destination at the appropriate temperature, even in adverse external temperature conditions.

•

17” high resistance rigid tube with polyurethane paint, with water inlet tap that also allows air insufflation, reducing the final weight of the equipment. The rigid tube has an anatomical design for greater comfort for the stallion during semen collection.

•

Latex mucosa with 1.2 mm thickness and high durability.

•

Disposable plastic mucosa for better hygiene during semen collection.

•

Collection cup coated with polyurethane, isothermal and protected from sunlight, providing ideal protection for freshly collected spermatozoa.

•

Reusable bags that allow optimizing the use of the collection cup and maintaining hygiene during the semen collection.

How to use For more information, access the QR CODE below : Presentation Styrofoam thermal box, 2 reusable ice packs, and 3 bottles of BotuIA.

BOTUFLEX CURVE (1 ICE)

Temperature (ºC)

The artificial vagina kit is composed of the following:

Time / Hours

BOTUFLEX CURVE (2 ICE)

Also available is an artificial vagina for fractionated semen collection. Indicated for the diagnosis and semen collection in cases of hemospermia and pyospermia.

•

Temperature (ºC)

Kit items can be purchased separately.

Open-ended model artificial vagina, 12” high resistance rigid tube with polyurethane paint, with water inlet tap that also allows air insufflation, reducing the final weight of the equipment.

The rigid tube has an anatomical design.

Time / Hours

BOTUAI BotuAI is a bottle for transporting cooled semen, which provides greater safety, practically and facilitates the identification of the shipped samples.

FOR ARTIFICIAL INSEMINATION

It avoids the use of plastic bags that may rip during transport, causing loss of the semen sample.

BotuAI’s lid has a design that makes it possible to directly attach the vial to the pipette, eliminating the use of syringes for artificial insemination.

21

Bovine semen extender

22

BOVINE SEMEN EXTENDER

BOTUBOV®

HIGHER PROTECTION & FERTILITY RATE FOR BOVINE CRYOPRESERVED SPERM.

BotuBov®’s formula contains innovative substances to protect bovine, buffaloes and small ruminants’ sperm integrity from cryodamages during the cooling and freezing process.

Researchers described higher Fixed-Time Artificial Insemination results for bull sperm cryopreserved in BotuBov compared to other extenders.

Furthermore, it can be used in FTAI programs with cooled semen. Scientific studies indicated: •

•

The use of BotuBov in association with the BotuFlex provides better sperm motility and a higher percentage of rapid spermatozoa in cooled bovine semen.

Sperm samples extended in BotuBov showed higher in vitro quality parameters (motility, viability and sperm membrane integrity ) during refrigeration at 5ºC for up to 48 hours, being used as an alternative in AI and FTAI programs for buffaloes.

Composition Sugars, antioxidants, amino acids, egg yolk and glycerol.

Storage and stability Store it frozen at -20ºC. After thawing, keep it refrigerated at 5ºC for up to 2 days.

Presentation Bottle containing 100 mL.

23

Canine semen extender

24

PROTECTION & HIGH FERTILITY

BOTUDOG FREEZING

FREEZING OF DOG SPERM

BotuDog[1] is an extender specially designed for handling, transport and artificial insemination of canine semen, with proven success.

Extender developed exclusively for the cryopreservation of canine semen, with high rates of post-thawing sperm motility and viability[1].

How to prepare Add the sachet’s content to the sterile water and shake well.

Exclusive formulation designed to protect the integrity of canine sperm.

How to use For cooled transport, dilute semen in a ratio of 2:1, 3:1 or 4:1 dilution (extender: semen) (v/v), according to the sperm concentration of ejaculate.

For better results in cooled semen transport, use BotuFLEX.

How to use After semen collection, centrifuge raw semen at 600X for 6 minutes. Discard the supernatant and resuspend the sperm pellet with BotuDog Freezing at an appropriate sperm concentration. Pack and seal the straws.

Stabilize the straws at 5oC for 1 hour.

Then, straws should be frozen in nitrogen vapor (Styrofoam box), 3 cm above the liquid nitrogen level for 20 minutes.

The straws must be placed in a water bath at 37oC for 30 seconds for thawing.

Composition Casein, sugars, preservatives, and excipients.

Storage and stability After mixing the contents of the sachet with sterile water, store it at 2 to 8oC for up to 3 days.

Presentation Pack of 2 diluent bottles containing 20 mL of sterile water and 2 sachets with 1.6g each.

CANINE SEMEN EXTENDER

BOTUDOG

Composition Sugars, antioxidants, amino acids, egg yolk, cryoprotectant and vehicle.

Storage and stability Store it frozen at -20ºC. After thawing, keep it refrigerated at 5ºC for up to 2 days.

Presentation Bottle containing 8 mL

BOTUDOG TURBO

IMPROVE THE SPERM MOTILITY

How to use For cooled transport, dilute semen in a ratio of 2:1, 3:1 or 4:1 dilution (extender: semen) (v/v), according to the sperm concentration of ejaculate.

For better results in cooled semen transport, use BotuFLEX. Composition Casein, sugars, preservatives, pentoxifylline, and excipients.

BotuDog Turbo[1] is an extender designed to improve the vigor of slow-moving sperm. The formulation contains pentoxifylline, a substance that increases the speed of sperm movement. Recommended for dogs with low sperm vigor.

BotuDog Turbo is indicated in the handling and transporting of canine semen and artificial insemination.

Storage and stability After mixing the contents of the sachet with sterile water, store it at 2 to 8oC for up to 3 days.

Presentation Pack of 2 diluent bottles containing 20 mL of sterile water and 2 sachets with 1.6g each.

How to prepare Add the sachet’s content to the sterile water and shake well.

23 25

2222 W Parkside Ln Unit 123 Phoenix AZ 85027 +1 (480) 490-2372 botupharmausa.com