Richter-Helm BioLogics GmbH & Co. KG

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Resolve BioSciences GmbH

Creative Campus Monheim Gebäude A03 Alfred-Nobel-Straße 10 40789 Monheim am Rhein Germany Joanne Dimitrakopoulos +49-2173-2975-200 info@resolvebiosciences.com www.resolvebiosciences.com

IL

72 2021

spatial biology, transcriptomics, neuroscience, oncology, cardiovascular, immunology, computational biology, developmental biology, microscopy, gene expression, infectious diseases, toxicology, drug development, biomarkers, organoids, atlasing, agrigenomics

Resolve Biosciences is enabling scientists to apply the power of Molecular Cartography™ technology to gain new insights based on the highest-resolution view of spatial biology. We operate two service laboratories: in Monheim am Rhein, Germany, and San Jose, CA, USA. We also began deploying commercial instruments at customer sites early in 2022.

Groundbreaking Technology

Under development since 2016, our pioneering platform and fully automated workflow feature proprietary, highly multiplexed, single-molecule fluorescence in situ hybridisation technology, which offers full spatial context at subcellular resolution in three dimensions. Unlike other approaches, our Molecular Cartography platform provides the required sensitivity, specificity, and workflow convenience to elucidate the cell’s complex transcriptional landscape without destroying the tissue section or cell culture sample.

This innovative approach was designed for the multiplexing needs of most scientists for basic, translational, and clinical research. The underlying technology is modular and flexible enough to incorporate the interrogation of DNA, protein, and metabolomic data layers in the future.

Advantages › Subcellular resolution – diffraction-limited, singlemolecule detection with a resolution of 300 nm › Highly multiplexed – each sample can be analysed by up to 100 different probe sets › Superior sensitivity – enables visualisation of millions of individual transcripts per sample › Transcriptomic reach – suitable for short transcripts down to 612 base pairs › Sample integrity – allows further interrogation using immunohistochemistry or fluorescent dye labelling

Context Matters

Visualization of gene expression gradients of 10 markers between portal and central veins in adult mouse liver. Applications › Cancer › Neuroscience › Developmental biology › Cell atlasing › Toxicology › Infectious disease › Agriculture

Research Highlights › SARS-CoV-2 Infection and Inflammatory Response — Medical University of Graz, Austria: Our Molecular

Cartography platform is enabling scientists to gain a better understanding of subcellular gene regulation and how the infection affects neighbouring cells over time. › Human Brain Development — MRC Laboratory of

Molecular Biology, Cambridge, UK: The team is applying the power of Molecular Cartography to brain organoids to unravel the spatial logic of neural tissue development. › Retina Gene Expression and Cell Type Evolution —

University College London, UK: The team is using

Molecular Cartography technology to better understand how a healthy retina develops and changes with advancing age. › Human and Mouse Liver Atlasing - VIB Ghent University, Belgium: Spatial proteogenomics revealed distinct and evolutionarily conserved hepatic macrophage niches. › Spatial organization of ependymal cells - Norwegian

University of Science and Technology, Norway: The team is using Molecular Cartography for the validation of ependymal cell spatial organization in the zebrafish brain.