Screening of Substrates, Inhibitors, and Other Ligands

Screening of Substrates, Inhibitors, and Other Ligands Creative Enzymes Inc.

Introduction of Substrates

In biochemistry, the substrate is a molecule upon which an enzyme acts.

Enzymes catalyze chemical reactions involving the substrate(s). In the case of a single substrate, the substrate bonds with the enzyme active site, and an enzyme-substrate complex is formed. The substrate is transformed into one or more products, which are then released from the active site. The active site is then free to accept another substrate molecule. In the case of more than one substrate, these may bind in a particular order to the active site, before reacting together to produce products.

Introduction of Inhibitors

Enzyme inhibitors are molecules that bind to enzymes and reduce their activity. By binding to the active site of the enzyme, the inhibitor reduces the compatibility of the substrate with the enzyme, thereby inhibiting the formation of the enzyme-substrate complex, preventing the catalytic effect of the reaction, and reducing (sometimes zero) the reaction . Because blocking the activity of enzymes can kill pathogens or correct metabolic imbalances, many drugs are enzyme inhibitors.

Introduction of Ligands

Ligand is a chemical term that refers to atoms, molecules, and ions that can bond with a central atom (metal or metalloid). Generally speaking, the ligand will provide at least one electron when participating in the bonding.

The ligand plays the role of Lewis base. But in a few cases, the ligand accepts electrons and acts as a Lewis acid. In organic chemistry, ligands are often used to protect other functional groups (for example, ligand BH3 can protect PH3) or to stabilize some easily reactive compounds (such as tetrahydrofuran as a ligand for BH3). The compound composed of the central atom and ligand is called a complex.

High-throughput screening

High throughput screening (HTS) technology is based on the experimental methods at the molecular level and the cellular level, using microplates as the experimental tool carrier, performing the experimental process with an automated operating system, and collecting with sensitive and rapid detection instruments.The experimental result data is analyzed and processed by computer, a large number of samples are tested at the same time, and the technical system supported by the corresponding database is running.

Computational Simulation Method

Computational simulation method is often used to analyze the physical movement of atoms and molecules. Allow atoms and molecules to interact within a fixed period of time, giving a dynamic "evolution" view of the system. In the most common version, the trajectories of atoms and molecules are determined by numerically solving the Newton equation of motion of the interacting particle system, where the forces between the particles and their potential energy are usually calculated using the interatomic electric potential or molecular mechanical force computational. This method is mainly used in chemical physics, materials science and biophysics.

Our services for screening of Substrates, Inhibitors, and Other Ligands

Creative Enzymes is a leading professional in the field of enzyme ligand screening. We are specialized in rapid and accurate evaluation of substrates, inhibitors, and other ligands for specific enzymes, based on high-throughput experiments or computational simulation results.

In other cases, we also provide screening of active enzymes against specific substrates or inhibitors, which would help to identify the functioning enzymes in a pathway or the biological target of a small molecule.

Screening Services Selection Guide Services

Highlighted Services

Substrate screening and identification

 Best substrate for an enzymatic rea Give the key information of the natural ction function of the enzyme.

Inhibitor screening and design

Target enzyme identification

Identification and screening of other ligands

 Natural substrates of an enzyme  Virtual screening of enzyme inhibit ors  High-throughput screening of inhibi tors  Structure-based inhibitor design  Ligand-based inhibitor design  Enzyme screening against substrat es  Identification of a target enzyme  Screening of enzyme activators  Evaluation of cofactors and additive s  Detection of enzyme aggregation an d oligomerization  Enzyme folding facilitators

Features

Helpful in drug discovery, fundamental research and other industrial applications.

Helpful in medical, diagnostic metabolomics.

Helpful for enzyme stabilization, storage, and optimization of reaction conditions.

Helpful in correcting enzyme folding and enzyme stability.

Our advantages Advanced: state-of-the-art candidates handling and tracking

Huge screening platform: 240,000 compounds, with 3 library subsets designed for specific screening needs.

Updated: each candidate is up-to-date, less than 2 years old at time of selection Reliable: candidates have been rigorously selected to exclude undesirable features Development-ready:Â all candidates or building blocks are available in trial sizes

Contact Us www.creative-enzymes.com Address: 45-1 Ramsey Road, Shirley, NY 11967, USA

Tel: 1-631-562-8517

Email: info@creative-enzymes.com

Fax: 1-631-938-8127

1-516-512-3133

THANKS Creative Enzymes Inc.