Native Bacillus licheniformis Protease

Nave Bacillus licheniformis Protease

Catalog Number NATE-0633

Descripon

A protease is an enzyme that helps proteolysis: protein catabolism by hydrolysis of pepde bonds. The nave Bacillus licheniformis protease belongs to the serine endopepdase sublisin family, which breaks the pepde bond non-specifically under alkaline condions. The enzyme is acve in aqueous soluons and in some organic solvents such as dry octane. The protease is inacvated by serine acve-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate.

Product Informaon

Catalog number: NATE-0633

EC number: 3.4.21.62

CAS number: 9001-92-7

Source: Bacillus licheniformis

Molecular Weight: 27 kDa

Form: Lyophilized powder

Acvity: >8 units/mg solid

Opmum pH: 8-10

Applicaons

Acve temperature: 4-75 °C

Opmum temperature: 55-60 °C

Specificity: Sublisin A is a member of the serine S8 endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 posion. It hydrolyzes nave and denatured proteins, and is acve under alkaline condions.

•The protease has a broad specificity towards nave and denatured proteins.

•The product has been used in ssue dissociaon and single cell isolaon from specimen during preparaon for single-cell RNA sequencing at cold temperatures.

•It has been used in the process of isolaon of subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria that can be used for funconal in vitro studies.

•The product has been used with other enzymes for in situ proteolysis to produce crystals suitable for structure determinaon.

Publicaons

Nave Bacillus licheniformis protease can be used for single-cell dissociaon at 4-8°C. The protocol involves mincing the specimen, digeson with protease on ice, incubaon with Miltenyi gentleMACS and trituraon or physical disrupon, followed by filtraon. Reported examples can be found in the following published research arcles:

Title

Probabilisc cell type assignment of single-cell transcriptomic data reveals spaotemporal microenvironment dynamics in human cancers

Ulcerave colis mucosal transcriptomes reveal mitochondriopathy and personalized mechanisms underlying disease severity and treatment response

Psychrophilic proteases dramacally reduce single-cell RNA-seq arfacts: a molecular atlas of kidney development