GLYCOMICS PROFILING GLYCOMICS ANALYSIS BY MASS SPECTROMETRY introduction of glycans Glycomics is the study of glycans that focuses on the the structure and funcďż˝on of carbohydrates. Glycoform distribuďż˝ons at the cellular, ďż˝ssue, organ and organism levels are also studied. Three aspects of glycomics are usually explored, including structural characterizaďż˝on of glycans, research of glycan-protein interacďż˝ons, and the study of in vitro and in vivo systems for the determinaďż˝on of the funcďż˝on of speciďŹ c glycans.
The majority of the linear sugars are glycosaminoglycans (GAGs) that consist of repea�ng disaccharide units. The units are 0-linked to a core protein, forming a proteoglycan aggregate.
Linear
Branched glycans are present as N-linked and O-linked glycosyla�on on glycoproteins or on glycolipids.
Îą3/6 Îą3/6 Îą8
Îą4
ι2 ι3 ι3/β4
Îą4
ι3 β4
Îą3
β3 ι2
Îą2
Îą2
Îą2 Îą6
Îą3
β4
β4 β2
Îą6
Îą3
β4
Îą3
Îą3
Îą6
β2
β4/6
β2
β4/6
β4
β4
β4
β4
β4
β4
β4
β4/6
Îą6 Îą3
β3 β3
β4 β3
Îą3
β6
β3
β3
β3
β4
β3
β6
Îą6
Asn
Asn
Hybrid
Complex
Asn
High mannose
Branched
Ser/Thr Ser/Thr
β3
Îą3
Îą4
β4
β4
β4
β3
Îą3
Îą4
β4
β4
β3
β3
β3
β3
β3
β3
β4
β4
β4
Ser/Thr Ser/Thr Ser/Thr
Ser/Thr Ser/Thr
Core 1 Core 2 Core 3 Core 4
N-linked glycans
β4
HS
O-linked glycans
CS
DS
Glycosaminoglycans ( Figure reference to Hyun J Y. et al., 2017)
Proteoglycans glycosaminoglycan
Ser/Thr
Glycoproteins
Glycosphingolipids
Ser/Thr
O-glycan
Glc Man
GalNAc Xylose
Gal NeuNAc
GlcNAc Fuc
N-glycan
Asn
general glycomics strategies
Ser Thr
Ser Thr
Asn
Asn
Diges�on
Ser Thr
Glycan
Asn
Asn
Body Fluids
Release
Asn
Ser Thr
Asn
Cells/Tissues
Glycolipids
Release
Glycan Release
Glycans
Glycosaminoglycans
Free Glycans
Deriva�za�on
HPLC Separa�on
Mass Spectrometry
b3 b2
Intensity
y2
Proteoglycans
Glycan
Glycopep�des
y3
Glycoproteins
m/z
services and advantages
N-Glycan / O-Glycan ProďŹ ling N-Glycosylaďż˝on / O-Glycosylaďż˝on Site Occupaďż˝on Analysis N-Glycan / O-Glycan Linkage Analysis Structural Characterizaďż˝on of Glycans
Technology Pla�orm: MALDI-TOF MS UPLC-FLD/MS HPAEC-PAD Microarray NMR spectroscopy
Glycopep�des Analysis
High Sensiďż˝vity and SpeciďŹ city
Glycans Microarray Assay
Stability and Consistency
Poysaccharide Analysis
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Glycan Microarray