Enzyme Labelling Service

Enzyme Labelling Creative Enzymes Inc.

Introduction of Enzyme Labelling

Enzyme labeling, also known as immunoenzymatic technology, is based on the immune reaction. It uses a highly specific immune reaction combined with the efficient catalytic action of enzymes to form a complete set of biological technologies that are highly specific and highly sensitive.

Development of enzyme labeling

The development of enzyme labeling mainly has two directions, one is enzyme immunoassay technology, the other is enzyme immunohistochemical staining technology. It is used to detect soluble antigen and antibody components in body fluids and to locate antigen or antibody components on cells and tissues. The reaction results of the enzyme labeling technology are easy to observe.

Commonly used labeling enzymes

Horseradish peroxidase, HRP

Alkaline phosphatase ďźŒ AP Glucose oxidase, GOD

Horseradish peroxidase, HRP

The molecular weight is small (40kD), and the marker can easily penetrate into the cell.

Good solubility. 5g HRP can dissolve in 100ml buffered salt solution, it can also be dissolved in ammonium sulfate solution below 62% saturation.

Cyanide, sulfide, fluoride and azide have inhibitory effect on HRP activity.

Alkaline phosphatase ďźŒ AP

Alkaline phosphatase (AP) markers are often highly polymerized macromolecules, which have poor penetrating cell membrane performance and are rarely used for immunoenzymatic histochemical localization studies. AP is mainly used for double-label staining to study the coexistence of transmitters and enzyme immunoassay. Glycine, citrate, EDTA, mercapto compounds can inactivate AP.

Glucose oxidase, GOD

In theory, COD is better than AP and HRP, because there is no endogenous GOD in animals, and there is little nonspecific interference. However, its molecular weight is relatively large (160 ～ 190kD), and it has more amino groups. It is easy to form a wide range of polymerization when labeling, which affects the activity of the enzyme.

Therefore, the sensitivity of GOD as a tracking enzyme is lower than that of HRP and AP, less hydrogen donors, and its application is more limited. It is mainly used for two enzyme coupling reaction amplification technology, which can improve the sensitivity and specificity of the method.

Requirements The enzyme used to label the antibody (or antigen) should meet the following requirements:

01 High purity and specific activity, and it is cheap and easily available.

properties, good 02 Stable solubility and easy to connect with antibodies (or antigens).

03 The substrate of the enzyme is not harmful to human health. The decomposition products are easy to measure, high sensitivity, and convenient color comparison

04 There is no endogenous enzyme or inhibitor the same as the labeled enzyme in the tested tissue or body fluid.

Featured Enzyme Labeling Categories

Featured Enzyme Labeling Categories

Our advantages Detection and purification of the desired enzyme.

The test results can be colorimetrically determined with the naked eye, microscope or spectrophotometer, and can be qualitative and quantitative.

Tracking of enzyme expression, localization, interaction, activity and conformational changes in complicated cellular signaling events.

The reaction results of enzyme labeling technology are easy to observe.

Our services Creative Enzymes offers enzyme labeling services both in vitro and in vivo. We have developed the enzyme labeling techniques to accommodate virtually any need for all types of biomolecular probes. With decades of experiences and unique technologies, Creative Enzymes makes commitment to providing customers with the highest quality enzyme labeling services.

Creative Enzymes has developed a series of enzyme labeling techniques and reagents to meet various research requirements. Enzyme labeling leads to the covalent attachment of different molecules, including biotin, reporter enzymes, fluorophores and radioactive isotopes, to the target enzyme. While multiple types of labels are available, their versatile uses are preferable for specific applications.

Contact Us www.creative-enzymes.com Address: 45-1 Ramsey Road, Shirley, NY 11967, USA

Tel: 1-631-562-8517

Email: info@creative-enzymes.com

Fax: 1-631-938-8127

1-516-512-3133

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