Enzyme Expression and Purification -Creative Enzymes

Enzyme Expression and Purification Creative Enzymes Inc.

What are probiotics?

“What are Enzyme? ”

Enzymes are proteins or RNAs that are produced by living cells and are highly specific and highly catalytic for their substrates. The catalysis of enzymes depends on the integrity of the primary structure and spatial structure of the enzyme molecule. If the enzyme molecule is denatured or the subunits are depolymerized, the enzyme activity can be lost. Enzymes are biological macromolecules, with a molecular mass of at least 10,000 and up to one million.

Structural characteristics Biological enzymes are produced from organisms, which have special catalytic functions and their characteristics are as follows:

High efficiency

Low reaction conditions

Using enzymes as catalysts, the catalytic efficiency of enzymes is 10^7~10^13 times that of general inorganic catalysts.

Enzyme-catalyzed reaction can be carried out at relatively mild normal temperature and pressure.

Specificity An enzyme can only catalyze the chemical reaction of one type of substance.

Mechanism of action

The mechanism of enzyme action is more recognized by Koshland's "inducible fit" theory, its main content is: when the substrate is bound to the active site of the enzyme, there is a change in the conformation of the enzyme. The correct orientation of the catalytic group is necessary for catalysis. The substrate induces a change in the conformation of the enzyme protein, leading to the correct positioning of the catalytic group and the binding of the substrate to the active site of the enzyme, and heavy metal ions will bind to the active site to inactivate the enzyme.

Our services Creative Enzymes has decades of experiences in enzyme expression and purification. We provide a series of services：

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Gene cloning

Expression

Fermentation optimization

Enzymes production

Our services

On the top of enzyme expression, we also provide the purification service for enzymes from natural sources or customized designs.

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Stable and full-fledged platform

Full range of expression systems

From 0.5 mg to > 100 kg capacity

Simultaneous screening of >16 conditions

Delivered > 200 custom enzymes

Consultation from cloning to purification

Our services-Enzyme Expression and Production Several steps are necessary to obtain a natural or recombinant enzyme depending on the source of the gene sequence and the specific technical requirement：

The initial cDNA synthesis, amplification, and isolation

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Insertion into a vector

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Induction and expression of the target enzyme

Our services-Enzyme Expression and Production When it comes to expression hosts, a wide variety of protein expression systems are available. Enzymes can be expressed in cell cultures of bacteria, yeasts, molds, mammals, plants or insects, or via transgenic plants and animals. Generally, bacteria and yeasts are the most developed systems, which means lost-cost expression and production; while higher organisms are more expensive to operate but also provide the capability to product more complex enzymes.

Advantages of typical hosts for enzyme expression.

Our services-Enzyme Expression and Production With decades of experiences in cloning and expression, Creative Enzymes offers several unique and advanced solutions for many types of enzymes. As one could expect from the previously mentioned key points of expression system selection, choosing the most suitable system for an enzyme greatly relies on the experience of the researcher, as well as thorough consideration of the enzyme properties.

Therefore, we encourage the customer to share as many details as possible when submitting the service request. Our service is as follows: •Expression evaluation and optimization •Bacteria • Escherichia coli • Bacillus • Other bacteria •Yeasts • Saccharomyces cerevisiae • Methylotrophs, such as P. pastoris and H. polymorpha (P. angusta) • Other yeasts •Fungi •Baculovirus/Insect cells •Mammalian cells •Transgenic animals •Transgenic plants •Cell free expression (in vitro expression) •Difficult-to-express enzymes • Toxic enzymes • Unstable enzymes • Peripheral enzymes (membrane protein) •Cell line/host development

Enzymes Expression Service Workflow 1

Service inquiry and technical consultation

2 Gene identification, synthesis and cloning

Creative Enzymes will best tailor these services based on customers’ research needs.

3 Codon optimization and expression system selection

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Prevention of sepsis in premature infants

5 Enzyme expression and conditions optimization, Purification 6 Result and report delivery

Our advantages

Creative Enzymes has a professional research and development team of probiotics.

Creative Enzymes has cutting edge facilities & innovative techniques.

Creative Enzymes can provide customers with comprehensive, professional, enzyme expression and purification.

Please feel free to contact us if you have any questions about enzyme expression and purification. We are glad to provide the most professional solutions.

Contact Us Address: 45-1 Ramsey Road, Shirley, NY 11967, USA

Email: info@creativeenzymes.com Tel: 1-631-562-8517 512-3133

Fax: 1-631-938-8127

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