Biocatalysis Services

Biocatalysis

Creative Enzymes Inc.

1

â—† Introductions

Biocatalysis is the use of living systems or their components to accelerate chemical reactions. In the process of biocatalysis, natural catalysts (such as enzymes)

chemically transform organic compounds. These two enzymes that have been more or less isolated and those that are still present in living cells are both used. The process of organic synthesis using natural or modified enzymes is called chemical enzymatic synthesis. Reactions performed by enzymes are classified as chemical enzymatic reactions.

2

Asymmetric Biocatalysis The use of biocatalysis to obtain enantiopure compounds can be divided into two different methods: 1.Kinetic resolution of a racemic mixture 2.Biocatalyzed asymmetric synthesis

In kinetic resolution of a racemic mixture, the presence of a chiral object (the enzyme) converts one of the stereoisomers of the reactant into its product at a greater reaction rate than for the other reactant stereoisomer. The stereochemical mixture has now been transformed into a mixture of two different compounds, making them separable by normal methodology.

3

Asymmetric Biocatalysis

Biocatalyzed kinetic resolution is utilized extensively in the purification of racemic mixtures of synthetic amino acids. Many popular amino acid synthesis routes, such as the Strecker Synthesis, result in a mixture of R and S enantiomers. This mixture can be purified by (I) acylating the amine using an anhydride and then (II) selectively deacylating only the L enantiomer using hog kidney acylase.

4

Significance of Biocatalysis

The natural, green biological catalysts attract fast growing attentions for many benefits, such as sustainability, high efficiency, and little waste footprint. However, developing and setting up such systems could be challenging, given that the nature of these biocatalysts is distinct from traditional ones. Furthermore, maintaining a biocatalytic mechanism performing as a stable and consistent manner also requires engineering design and development on the whole system, in addition to the molecular biology and biochemistry techniques that are essential to start feasibility evaluation on the biological catalysts.

5

â—† Our Services

Creative Enzymes presents the professional development platform for biocatalysts by integrating the expertise on molecular biology and protein engineering, and experience on development of industrial processes. Creative Enzymes has extensive partnership with academic institutes working at the cutting edge technologies of biocatalysis. This enables Creative Enzymes to provide the latest solutions and unbiased selection of the best products for our clients.

6

Catalytic Assays

Catalytic assays are performed to determine the amount of the enzyme or substrate in a sample quantitatively. Also, it can be used to prove presence or absence of the enzyme in a distinct specimen, like an organism or a tissue, as a qualitative approach.

Creative Enzymes offers the service of catalytic assays for the best evaluation of target biocatalysts. We have extensive experience with various types of biocatalysts and therefore offer professional one-stop services: •Reaction condition optimization.

•Catalytic assay development and validation.

•High-throughput strategies design.

•Assay evaluation.

7

Computational Modeling

In the process of identification and design of a biocatalyst, experience and skills are often required along with tedious cycles of design-trialoptimization. Computational modeling technique is an alternative way from traditional experimental methods to study bioactive molecules for target biocatalysts.

Creative Enzymes’ service of computational modeling helps customer find the most suitable substrates for their target. With variety of in silico tools, the professional services provide much insight into the desired biocatalysts at a relatively low cost: •Chemical database search.

•Three-dimensional structure prediction and determination.

•Virtual screening of substrates.

•Rational design of the biocatalyst.

8

◆ Substrate Profiling

Substrate selectivity or specificity is the key to successful development of biocatalysts. As a natural catalyst, enzyme inherently presents promiscuity over similar molecules. Therefore, understanding substrate specificity of a particular enzyme holds an important place in Biocatalysis development.

Creative Enzymes offers the service of substrate profiling, aimed at evaluation of substrate specification of a known biocatalysis reaction. Enabled by our best knowledge of bioinformatic technique, we offer professional one-stop services: ⚫ Substrate assay design and validation. ⚫ Synthetic peptide library construction. ⚫ MSP-MS assay. ⚫ Data analysis and development planning

9

Other Services

◆ Our Features

10

•

Biodegradable and

•

through minimization of side

environmental friendly reagents

reactions, by-products and

and solvents •

Services and consultation tailored to specific development needs

•

impurities •

Optimization and enhancement of conversion rates, yields, and

Efficient synthetic and processing routes

Decreased cost and turnaround time

selectivity

•

Design and setup of selfregenerating or low-maintenance systems

Get In Touch

Email: info@creative-enzymes.com

Tel: 1-631-562-8517 1-516-512-3133

Address: 45-1 Ramsey Road, Shirley, NY 11967, USA Fax: 1-631-938-8127

THANKS!