Saniguard 7500. Silane quat antimicrobial.99.99% all surface protection from Human-Carona virus

SANIGUARD-7500 SILANE QUAT ANTIMICROBIAL

SANIGUARD-7500

Saniguard-7500 QUATERNARY AMMONIUM SILANE FOR ANTI-MICROBIAL EFFICACY

 Odorless  Eco-responsibility  Surface compatibility  Broad spectrum - shields the surface against bacteria, viruses, molds, fungi, yeast and protozoa  Stable on storage  Non- flammable  Retentive for 90 days (under simulated conditions)  Non-toxic and safe  Cost-Effectiveness

A UNIQUE ANTIMICROBIAL TECHNOLOGY Antimicrobial, Disinfectant, Biocide, Sterilant, Biostatic and Odor Eliminator

Is based on advanced silane chemistry : the organo silane technology is proven to be very effective It is a is the next generation retentive “ anti-microbial � technology that works on all surfaces with the added benefit of applying through water solution.

Traditional antimicrobials provide short-term kill of microorganisms.

Unlike disinfectants, sanitizers and bleaches, the water based Saniguard-7500 forms a molecular bond to surface. The molecule disrupts the cell membranes of harmful fungi and bacteria, inhibiting, reducing and providing long-term, residual kill.

It can produce a durable, non-migrating antimicrobial finish to a wide range of textiles. SANIGUARD-7500 can form an invisible and durable micro-biostatic coating on a broad range of hard surfaces.

The physical, rather than chemical, mode of action of SANIGUARD-7500 does not affect normal skin flora, does not lose strength with use and does not promote adaptative organisms (super bugs)

Sword That Punctures The Cell Membrane Of All Microbes

Longer lasting, more effective, versatile, non-toxic and save SANIGUARD-7500 : Mode of action

Part I : The first part of the molecule is the silane base. This silane base is the antimicrobial anchor. The antimicrobial is anchored by covalent bonds which are formed gradually through hydrolysis reactions that bond the antimicrobial permanently to almost any surfaces and allow for crosslinking and polymerisation with other molecules.

Part II : The second part of the molecule is the centrally located positively charged nitrogen. It plays an important role in the active nature of the antimicrobial. Some membranes of microbes are negatively charged; when in close proximity, the microbes are drawn into the active surface of the antimicrobial and Part III : The third segment is the edge that leads to blow in the offending microbes. This long molecular chain acts like a sword that punctures the cell membranes of all microbes coming in contact with it.

Longer lasting, more effective, versatile, non-toxic and save SANIGUARD-7500 : Mode of action When a microorganism comes in contact with the treated surface, the C-18 molecular sword punctures the cell membrane and the electrical charge shocks the cell. As the microbe is further drawn into the antimicrobial, it is electrocuted

Since nothing is transferred to the now dead cell, the antimicrobial doesn’t lose strength and the sword is ready for the next cell to contact it.

The active layer remains intact, efficacious and ready for the next cell that approaches.

Summary When the antimicrobial is bonded to the surface of an object, it is ready for defense.

As the microbes come into contact with the “antimicrobial” surface, they are first punctured by the long molecular chain.

The “positive-negative” attraction in between the positively charged nitrogen and the negative cell membrane draw the microorganism even further down on the sword.

This modified cell can end the life of a microbe but the end point is the contact between the negative and the positive charges : the cell membrane is electrocuted and blown apart delivering inescapable kill In addition, since this process transfers nothing from the antimicrobial to the cell, the antimicrobial stands ready for the next “wave” and can continue to work at full strength and definitely.

SANIGUARD-7500 – Bonding Mechanism

Applied in a single stage of the wet finish process, the attachment of SANIGUARD-7500 to surfaces involves two means.

First and the most important is a very rapid process, which coats the substrate , with the cationic species (physics sorption) one molecule deep. This is an ion exchange process by which the cation of the silane quaternary ammonium compound replaces protons from water or chemicals on the surface.

The second mechanism is unique to materials such as silane quaternary ammonium compounds. In this case, the silanol allows for covalent bonding to receptive surfaces to occur (chemisorptions). This bonding is then made even more durable by the silanol functionality, which enables them to homopolymerize. After they have coated the surface in this manner, they become virtually irremovable, even on surfaces with which they cannot react covalently.

1. Molecule used in SANIGUARD-7500 2. Surface view

3. Treated surface

We have 2 reactions: 1. The product forms a molecular BOND to any fabric 2. Product forms a film : crosslinking of the product

SANIGUARD-7500 – Application

Saniguard 7500 : Solution Preparation : For exhaust & spray Add one to three part of Saniguard 7500 to 97-99 parts of water and stir the solution for 5 min before using.

Use the solution after preparation within 7 days Do not use as hand sanitizer or spray on humans, animals and avoid inhalation

SANIGUARD-7500 – Application on Surfaces High Touch Surfaces

Coatings & Surface Treatments

Textiles

Construction Material

SANIGUARD-7500 – Application on Surfaces

SANIGUARD-7500 – Application Classification Textiles Household goods

Construction material

Footwear Interior goods Home electric appliances Others

Application Socks, underwear, dress-shirt, working uniform, formal dress Bedding & Night clothes (filling of a quilt, quilt cover, sheet, pajamas, blanket) Gloves, hat, handkerchief, apparels, mask etc Kitchen goods (towel, dish cloth, chopping board, water purifier, sponge), Polyurethane foam for household, industrial and institutional sponges and mops.

Bath room & toilet goods (bathtub, washbowl, bath mat, toothbrush, toilet fixtures, toilet seat cover, tiles, granite, paints, wood , sunmica, glass, plastic etc) Paints, granite, wood, metal, plastic, glass, marble, ceramic, rubber, resins, cement surfaces etc, Roofing materials – defined as shingles, roofing granules, wood shake, felt stone & synthetic overcoats.Sand bags, tents, tarpaulins, sails and ropes, jutes, Fiber glass duct board.

Shoes, Slippers, shoe insole Carpet, curtain, upholstery.

Vacuum cleaner, air cleaner, washing machine, telephone, air conditioner, headphones, floppy disk, key board, phones,switches , humidifier belts etc Stationary (Ball-point pen, file-cover, folder).

Cards (telephone card, cash card). Watch-strap, belt etc cell phones.

Computers, tabs, scrubs, masks, medical textiles, PPEs, medical equipment’s, stethoscopes etc.

Disposable polyurethane foam cushions for lavidus air float systems.

Polyurethane foam for household, industrial and institutional sponges and mops.

Polyurethane foam for packaging and cushioning in non-food contact applications Polyurethane foam used as a growth medium for non-food crops and plants

SANIGUARD-7500 – Application Hard and finished, both porous and non-porous surfaces, all medical equipment, medical instruments and devices, medical furniture, glass, rubber, stainless steel, concrete, plastic, drywall, marble, ceramic, supplies, medical textiles garment and much more!.

All Medical Equipment Saniguard 7500 is a nano-biostatic surface coating which protects against harmful bacteria and viruses. Durable, invisible, odourless, and will not affect the performance of the equipment.

Office and Work Spaces: Furniture, High touch surfaces, HVAC, Ventilation, Heating and Air Quality, Washrooms, Showers, Changing Areas, Break rooms, Lobbies and Common Meeting Areas.

SANIGUARD-7500 – Application In Healthcare: Hospitals, Operating Theater, Medical Instruments, Patient Rooms, Medical Furniture, Hospital Common Area, Washrooms, Showers, Door, Windows, Floor, Walls, Clinics, Chiropractor Offices, Physical Therapy Facilities, Massage Centers, Dental Offices and Extended Care Facilities, Ambulance and Patient Transportation, Medical Equipment and Devices, Medical Clothing, Scrubs, Lab Coat, Towels and Washcloths, Bottom Sheets, Sleep Knit Envelope Sheets, UltraKnit, Envelope Sheets, Privacy Curtains, Shower Curtains, Stretcher Sheets, Bariatric Sheets, Crib & Bassinette Sheets, Birthing Bed Sheet, Ultra Knit and Sleep Knit Top sheets, Ultra Knit and Sleep Knit Pillowcases, Woven Flat Sheets & Pillowcases, Peri Cloths, Aprons, Impermeable Aprons, Discloths and Tea Towels, Tablecloths, Napkins and Placemats, Color Grid, Knit Contour, Thermal Spread Blanket, Spread, Thermal Blanket, Spread, Thermal Blanket, Ether Blanket, Patient and IV Gowns, Patient Gowns, Precaution Gowns, Aurorra Clothing Protectors, Perma Print Clothing Protectors, Terry & Twill Clothing Protectors, Synthetic Bedpads, Aurorra Bedpads, Chamonix Bedpads, Meridian Bedpads, Sonoma Bedpads, Cotton Bedpads, Chairpads, Laundry Bags, Vinyl Laundry Bags, Mesh Laundry Bags, Zippered, Mesh Laundry Bag, Ward Carts, PVC Mesh Bags and Cart Covers, Soiled Linen Laundry Carts and High touch surfaces and more.

SANIGUARD-7500 – Application Customized Solutions: Schools, University, College, Libraries, Spas and Aesthetic studios, Community centers, Vet Clinics, Malls and Shopping Centers, Entertainment, Buses, Trians, and other forms mass transportation, Government Facilities, Public Transportation, Hotels, Restaurant, Airports and Airplanes.

Sports and Recreation: Gyms and Fitness Facilities, Equipment, Washrooms, Showers, Locker Rooms and Changing Areas, Steam Rooms, Hot Tubs, Swimming Pool, and Other Aquatic Areas, Artificial Grass and Turf, Yoga studios, High Touch Surfaces and much much more!

SANIGUARD-7500 – Application Implementation Saniguard 7500 can be seamlessly integrated into the Hospital acquired infections (HAI), Work acquired infections (WAI) and cross-contamination reduction protocol in the following ways: •

Disinfection using non-mutagenic and water-based chemicals with shortest kill times.

•

Surface protection following disinfecting regimen by spraying or wiping down the surface with water- based Saniguard 7500 solution. This includes un-obvious hotspots such as elevator buttons, handrails, countertops, office equipment, medical test equipment, cell phones, computers Tabs, etc.

•

Spraying all surfaces such as walls and ceilings to allow Saniguard 7500 treatment to scrub the air by applying in Air-handling units (AHU), Air-vent filters, HVAC systems etc.

•

Specifying material treatment of textiles in hospitals including scrubs, bed-linens, curtains, towels, mattress covers, gowns, Masks, etc.

SANIGUARD-7500 – Application Implementation Contd •

Replenishing treatments on a regular schedule.

•

Specifying Saniguard 7500 treatment, where possible, at the OEM level in key contamination vectors such as stethoscopes, medical device housings, textile furnishing, glass windows, distancing barrier sheets, Face shields, cough-guards, nurse-stations, reception areas etc.

•

Applying Saniguard 7500 dilutions (aqueous or in alcohol) on all Rigid-Flexible, PorousNonporous surfaces i.e plastics, wood, metal, rubber, paper, ceramics, stone ,porcelain, glass, leather, textile, garments, etc.

•

Replenishing treatments on a regular schedule.

•

Specifying Saniguard 7500 treatment, where possible, at the OEM level in key contamination vectors such as stethoscopes, medical device housings, textile furnishing, glass windows, distancing barrier sheets, Face shields, cough-guards, nurse-stations, reception areas etc.

•

Applying Saniguard 7500 dilutions (aqueous or in alcohol) on all Rigid-Flexible, PorousNonporous surfaces i.e plastics, wood, metal, rubber, paper, ceramics, stone ,porcelain, glass, leather, textile, garments, etc.

SANIGUARD-7500 – Application Method Can be applied by: Padding , exhaust , Soaking, Spraying , Fogging, Dipping, Brushing or wiping SURFACE APPLICATION METHOD: Clean the solid surfaces with mild detergent and water before spraying Saniguard 7500 solution.

Spray the solution on surfaces using a high-pressure mist sprayer/fogger only with dilution to ensure the deposition of finer atomized droplets and better surface coverage. Before mist spray check the effect and re-wetting on a small trial area before proceeding. If you experience a sticky surface then increase the dilution and after misting hand mopping should be done.

For very smooth granite, marble, Kota stone, SS surfaces, and smoothly coated walls, application with hand mopping is recommended.

High-pressure mist sprayer/fogger/hand mopping is recommended for exterior application & For interior application fogger & hand mopping is recommended.

SANIGUARD-7500 – Application Method

SURFACE APPLICATION METHOD Contd: Allow the treated surface to dry at room temperature overnight . The treated surface now has a retentive disinfectant layer.

Note Saniguard 7500 active has reactive Silane quat and forms a bonded protective structure on the surface to be coated. Hence always do a trial with the suggested applicator and method before usage.

Use facemask, goggles and gloves before applying the product TEXTILE APPLICATION METHOD: All clothing and other textile products should be treated by padding or exhaust (soaking ) them for at least 15 minutes in Saniguard 7500 solution, followed by wringing or squeezing the textile material and drying at room temperature. Use Saniguard 7500 solution post detergent wash.

SANIGUARD-7500 – Application Method

SANIGUARD-7500 – Application Method

SANIGUARD-7500 – Application in Non Textile Air control : Filter & Air Ducts (metallic materials) Concrete industry Paints AIR CONTROL : Filter & AIR DUCTS (metallic materials) SANIGUARD-7500 on FILTER Provides an invisible durable microbiostatic coating on most substrates from a water-based system.     

Inhibits the growth of odor causing bacteria.

Prevents the growth of mold/mildew and algae.

Is compatible with many processes and conditions, including pH from 3 to 10.

Attacks malodors at their source to prevent decay.

Can be incorporated into the manufacture of a wide variety of materials, providing extended life and usefulness to many products.

Uses Air purification systems.

Automobiles.

Re-circulating air handling systems.

Vacuum cleaner filters.

Aquariums.

Application level : Generally applied by spray : a 1-3% solution

SANIGUARD-7500 – Application in Non Textile Has 6 months protection against mold, mildew, fungus and algae Is superior protection from moisture penetration and the effect of weathering Can be cleaned up easily , and is non-toxic to humans, animals and vegetation Resists staining and water penetration and even resists spider webbing Dries to the touch in 1 to 2 hours, may be recoated in 3 to 4 hours depending on condition Application level Spray over air ducts(metalic materials) Mix thoroughly before use, easily applied with a quality brush, roller, airless spray and low pressure hand pump sprayer Always apply material to test panel prior to application Dry to the touch in 1 ¡V 2 hours, may be recoated in 3 ¡V 4 hours depending on condition Dilution Ratio 1 : 5 pure water. 200 litres = 1,000 litres of ready to use(RTU) products CONCRETE INDUSTRY SANIGUARD-7500 in Concrete Industry Prevents deterioration due to fungi (mold and mildew), bacteria and algae.

Prevents stains caused by fungi (mold and mildew), bacteria and algae.

Prevents odors caused by fungi (mold and mildew), bacteria and algae.

Prevents deposition of dust on the treated surfaces.

Is easy to apply and more effective than traditional antimicrobial products.

SANIGUARD-7500 – Application in Non Textile Application level 30 ml to 90 ml / litre of water. This amount shall be included in total water of the concrete mix design (for wet mix concrete).

Paints Industry Saniguard 7500 can be used in wood , decks painting, latex indoor and outdoor paints and stains; woodstains, architectural paints; lacquer and maintenance coatings; films; laminates and finishes including alkyd, urethane, enamel, epoxy, siloxaline, amino resins, textile coatings, extrusion coatings, architectural coatings and overlays, anti-corrosion coatings fire-resistant coatings, aliphatic coatings, vinylester and polyester coatings, gel coatings, aminoresins, resins used as additive mixes for cement, epoxy laminating resins.

By incorporating SANIGUARD-7500 in the Wood for wood furniture will inhibit deterioration and damage cause by these microorganisms for an extended period of time.

SANIGUARD – 7500, Analysis Of Application 1. Analysis of application of saniguard-7500 Correct Application of SANIGUARD-7500 can be checked by two means.

1. Bromophenol. Blue indicator-indicates the presence of SANIGUARD7500 by a blue cast. If no SANIGUARD-7500 is present, no color will form.

2. Phydron quaternary Test Strips QT-10 theses strips indicate the presence of a quaternary compound. These can be used to determine the effectiveness of the bath treatment. Use them as you would pH paper and read the amount of quaternary compound, if any that has been left in the bath.

2. To prevent yellowing of the fabrics where saniguard-7500 is used To prevent the Discoloration or yellowing of fabrics it is necessary to control the pH of the bath. Alkalinity may prohibit SANIGUARD-7500 from attaching to fabric. PH of fabric should be 4.5- 5.5 to aid exhaustion.

Different Acids solution can be used for this process. The most common used are: • Acetic Acid • Citric Acid

How to determine if SANIGUARD-7500 is present ?

Bromophenol Blue (BPB) stain test  Soak the treated substrate in BPB for 5 mins (0.04% solution in Water/ Methanol 80/20 )  Rinse the substrate with copious amount of water  Compare the remaining blue stain on the substrate to the untreated control and color standard (to be done)

OVERTREATED SLIGHTLY OVERTREATED GOOD TREATMENT SLIGHTLY UNDERTREATED UNDERTREATED

Saniguard-7500 Safety Non-toxic and safe Effectiveness Broad-spectrum efficacy against bacteria, viruses, molds, fungi, yeast and protozoa Cost-Effectiveness It is competitive in cost and a significant improvement over current offerings in effective cost when potential for recontamination or cross-contamination are factored.

Currently, non-leaching anti-microbials represent the best available solution. Within these, certain silver and copper-based chemicals have shown limited anti-viral activity, discolouration and have been expensive. Nano- silver has not been evaluated for long-term health and environmental issues.

Si-Quats have proven efficacy, non-mutagenicity and durability. Earlier versions of these were limited in their applicability and safety because of solvent content. This restricted use in mainstream cleaning and protecting regimens. Over the years, innovations in process chemistry have allowed Saniguard 7500 to be diluted into stable water-based formulations. This has allowed all of the efficacy, non-mutagenicity and durability benefits of the Si-Quat chemistry with safety and eco-responsibility.

This allows Saniguard 7500 be easily applied as fabric treatments, protecting solutions and surface treatments for contaminations discussed earlier such as cell phones. Computers, tabs, scrubs, masks, medical textiles, PPEs, medical equipment’s, stethoscopes etc.

Saniguard-7500 Durability: Durable and continue to act effectively for several days to weeks to months to life of the substrate (depends on application methods) – it protects contamination vectors between cleaning regimens and is durable enough to resist removal by these regimens be they disinfection, sanitization or laundering Non-mutagenicity Does not create super-bugs by using a physical killing mechanism and thus removing the poisoning cycle that leads to resistance in microorganisms Eco-responsibility It is water-dilutable and has no volatile organic emissions (VOCs) and have no adverse environmental impact during its entire life cycle.

SANIGUARD-7500 – Effectiveness The following table lists the pathogens that are killed or inactivated following application of Saniguard 7500 to various substrates and materials.

PATHOGENS INACTIVATED BY SI-QUAT APPLICATION Gram Positive Bacteria Bacillus sp. (vegetative cell) Corynebacteriumdiptheriae Micrococcus lutea Micrococcus sp.

Mycobacterium tuberculosis Mycobacterium smegmatis Propionibacterium acnes Staphylococcus aureus * Staphylococcus epidermidis Streptococcus faecalis Streptococcus mutans Streptococcus pneumonia Streptococcus pyrogenes

Gram Negative Bacteria Acinetobacter calcoaceticus Aeromonas hydrophilia Citrobacter deversus Citrobacter freundi Enterobacter aerogenes Enterobacter aglomerans Enterobacter cloacae Enterococcus Escherichia coli Klebsiella oxytoca Klebsiella pneumoniae Klebsiella terriena Legionella pneumophila Morganella morganii Proteus mirabilisl Proteus vulgaris Pseudomonas aeruginosa Pseudomonas fluorscens Salmonella cholera suis Salmonella typhi Salmonella typhimurium Serratia liquifaciens Serratia marcescens Xanthomonas campestris

Viruses Adenovirus Type II & IV Bovine Adenovirus Type I & IV Feline pneumonitis Herpes Simplex Type I Herpes Simplex Type II HIV-1 (AIDS) Influenza A2 (Aichi) Influenza A2 (Asian) Influenza B Mumps Parinfluenza (Sendai) Rous Sarcoma Reovirus Type I Simian Virus 40 Vaccinia MS2 PRD1

Viruses

Fungi , Algae , Yeast

SANIGUARD-7500 – Effectiveness The following table lists the pathogens that are killed or inactivated following application of Saniguard 7500 to various substrates and materials.

Fungi, Algae, Mould, Yeast, Spores Alterania alternate Aphanizomenon sp.

Aspergillus flavus Aspergillus niger Aspergillus sydowi Aspergillus terreus Aspergillus versicolor Aspergillus verrucaria Aureobasidium pullans Candida albicans Candida pseudotropocalis Chaetomium globsum Cladosporium cladosporioides Chlorella vulgaris Dreschslera australiensis Epidermophyton sp.

Gliomastix cerealis Gloeophyllum trabeum Microsporum sp.

Microsporu maudouinii

Monilia grisea Oscillatoria Penicillium chrysogenum Penicillium commune Penicillium funiculosum 14 Penicillium pinophilium Penicillium variable Phomafimeti Pithomyces chartarum Poria placenta Scenedesmus Saccharomyces cerevisiae Scolecobasidium humicola Selenastrum sp.

Trichoderma viride Trichophyton interdigitale Trichophyton maidson Trichophyton mentogrophytes Trichophyton sp.

Protozoa Parasites Cryptosporidium parvum (oocysts)

SANIGUARD-7500 – Test Antimicrobial Activity Evaluation Methods 1. MIC 2. AATCC Test Method 30 3. AATCC Test Method 90 (Halo Test) 4. AATCC 100 / ISO 20743 / ISO 20645 5. Modified AATCC 100 with MS2 Bacteriophage (Antiviral for textiles) 6. JIS Z 2801 / ISO 22196 7. JIS L 1902 8. ISO 18184(Antiviral for textiles)

SANIGUARD-7500 Features of Various Antimicrobial Agents for Textile Classification

Treatment Method

Durabilit y

Safety (LD50)

Leach/No n-leach

Saniguard 7500

Dilute with water

Excellent

12,270mg/kg

Non-leach

Quaternary Ammonium Compounds

Mix with reactive resin for fiber

Fair

300-400 mg/kg

Leach

Biguanide

Mix with reactive resin for fiber

Chitin, Chitosan

Mix with reactive resin for fiber

Fair

>5,000mg/kg

Leach

Inorganic Silver

Mix with raw materials of synthetic fiber

Excellent

>2,000mg/kg

Slowrelease

Natural extract

Microcapsule

Good

>2,000mg/kg

Slowrelease

MODEL OF BACTERICIDAL ACTION

Fiber

Inoculum

Fiber Saniguard-7500 treatment Inocumum

Solved agent

Survived bacteria

Death bacteria Death bacteria

(a) Water soluble type

(b) Water non-soluble type [Saniguard-7500]

SANIGUARD-7500 – Test

Untreated Sample

LEACHABLE Leaches out of substrate to inhibit growth

DURABLE Saniguard-7500 Durably bonded solid* that inhibits growth by physical contact * The solid is a micro polymer network

Sample of Leaching Antimicrobial

Bonded Antimicrobial

SAFETY OF SANIGUARD - 7500 Acute Oral Toxicity/LD50 One of the most typical toxicity evaluation tests in case of ingestion.

Test method/Using albino rats belong to mouse and feeding with test samples. The number of fifty percent death was calculated from the dead rat number statistically

Acute Oral Toxicity/ LD50 Saniguard 7500 12.27 g/kg Salt 3.0 g/kg Sorbic acid (food additive agent, disinfectant) 10.5 g/kg Caffeine (coffee, tea) 0.19 g/kg Sodium nitrife (a color former, disinfectant) 0.085 g/kg Ethylacohol (wine, whisky) 14 g/kg

SAFETY OF SANIGUARD - 7500 Acute Oral Toxicity

Albino Rats

Acute Dermal Toxicity

Albino Rat

Eye Irritation

Albino Rabbits

Primary Skin Irritation

Albino Rabbits

Four-Day static Fish Toxicity Studies

Rainbow Trout and Bluegills

Human Repeated Insult patch Test

Albino Rabbits

28-Day subacute Dermal Toxicity study Acute vapor Inhalation Toxicity study

Albino Rats

Host mediated assay for detection of mutations induced Three week vaginal irritation study

Beagle dogs

32-day human wear test for socks Three month human wear test for athletic socks Teratogenic study

Albino Rats

Mutagenicity evaluation In vitro mammalian cell transformation assay in the absence of exogenous metabolic activation Acute toxicity

Daphnia magna

Percutaneous absorption

Rabbits

Eight day dietary

Mallard duck

Acute oral toxicity

Mallard duck

SAFETY OF SANIGUARD - 7500 Sock wear test/3 month human wear test Test method: Socks made of wool, cotton, nylon, acrylic were treated with Saniguard-7500 and worn by 44 young men, American football player, for 3-month continuously Test result: No observation of skin irritation or skin sensitization Percutaneous absorption test Test method: Backs of rabbit wear clipped free, aqueous solution containing 14C-labeled Saniguard 7500 was applied. The Rabbits were kept for ten days. 14C contents in urine and feces were analyzed everyday. After ten- test-day, whole liver, kidneys, lung, spleen, brain and gonads were removed and 14C content were analyzed.

Test result: No observation of 14C in urine, feces and all organs. It proved that Saniguard 7500 was not absorbed from the skin of rabbits.

Saniguard 7500 Antimicrobial Activity on Hard Non Porous Surfaces- JIS-Z 2801:2012

1.Ceramic Tile 2.Granite 3.Sunmica 4.Wood

Name of Test : Test Method for Antimicrobial Activity of Hard Non-Porous Surfaces – JIS Z 2801: 2012 Test Organisms : Staphylococcus aureus ATCC 6538 & Escherichia coli ATCC 8739 Test Conditions : Contact Time : Incubation Temp Neutralizer Used Media and Reagent Incubation Period

24 Hours at 35°C +/- 1°C 2. : 35°C +/- 1°C 3. : D/E Neutralizing Broth : TSA Agar : 48 Hours

Note : A Value of 2.0 or above is considered “antimicrobial” by JIS Where, R=Value of antimicrobial activity A = Average of the number of viable cells of bacteria immediately after inoculation on the untreated test piece.

B = Average of the number of viable cells of bacteria on the untreated piece after 24 hrs.

C = Average of the number of viable cells of bacteria on the treated piece after 24 hrs Remark : The sample shows antibacterial activity against both S. aureus & E. coil.

Results: Sample Identification Treated Un-treated Treated Un-treated

Parameter

Treated Un-treated Treated Un-treated

S. aureus

Treated Un-treated Treated Un-treated

S. aureus

Treated Un-treated Treated Un-treated

S. aureus

Treated Un-treated Treated Un-treated

S. aureus

Treated Un-treated Treated Un-treated

S. aureus

S. aureus E coli

E coli

E coli

E coli

E coli

E coli

ACRYLIC, 2% Saniguard 7500 Bacterial count after Log of 0 Bacterial count after 24 0 hr (Cfu / Sample ) hr hr (Cfu / Sample ) 100 2 7600 420 2.623 820000 520 2.716 20000 930 2.968 3400000 WOOD , 2% Saniguard-7500 350 2.544 1 520 2.716 770 280 2.447 1 620 2.792 810 CERAMIC TILE , 2% Saniguard 7500 61000 5.785 111000 4800000 6.681 35000000 4000000 6.602 31000 77000000 7.886 5500000 GRANITE , 2% Saniguard 7500 140 2.146 1 2400 3.380 340 1200 3.079 1 3500 3.544 2000 PLASTIC SHEET, 2% Saniguard 7500 50 1.698 1 60 1.778 180000 80 1.903 35000 590 2.770 1050000 SUNMICA , 2% Saniguard 7500 1 0 1 130 2.113 110 680 2.832 1 950 2.977 600

Log of 24 hr 3.880 5.913 4.301 6.531

Anti –microbial(R) R=[Log(B-C)] 2.02

0 2.886 0 2.908

2.8

5.045 7.544 4.491 6.740

2.49

0 2.531 0 3.301

2.5

0 5.255 4.544 6.021

5.3

0 2.041 0 2.778

2.04

2.23

2.9

2.24

3.3

1.5

2.78

Saniguard 7500 Antimicrobial Activity on Textile as per Modified AATCC 100:2012, MS2 Bacteriophage

ďƒź Keep surfaces hygienic for longer without the hassle of sterilizing every day!

Using Saniguard 7500 Guard All Surfaces

L N CHEMICAL INDUSTRIES

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