5-(Aminomethyl)-1,2-oxazol-3-one (Muscimol) [C4H6N2O2]

Query Query

Results

Date

2 substances in Reaxys

2018-08-03 20h:31m:41s (UTC)

O

NH

1. Query

O

NH 2

Search as: As drawn ) AND (IDE.XRN=774694) OR (IDE.XRN=14819161))

Copyright © 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

1/94

2018-08-03 20:32:29

Reaxys ID 774694 View in Reaxys

1/2 CAS Registry Number: 2763-96-4 Chemical Name: Muscimol; 5-(aminomethyl)-2,3-dihydro-1,2-oxazol-3-one; [3H]-muscimol; musimol; 5-aminomethyl-isoxazol-3-one; 5-(aminomethyl)isoxazol-3-ol; 5-(aminomethyl)-3-(2H)-isoxazolone Linear Structure Formula: C4H6N2O2 Molecular Formula: C4H6N2O2 Molecular Weight: 114.104 Type of Substance: heterocyclic InChI Key: ZJQHPWUVQPJPQT-UHFFFAOYSA-N Note:

O

NH O

NH 2

Substance Label (10) Label References M

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys

SUP-1A

Patent; THE TRUSTEES OF COLUMIA UNIVERSITY IN THE CITY OF NEW YORK; WO2008/143876; (2008); (A2) English, View in Reaxys

table

Myhrer, Trond; Nguyen, Nga H. T.; Enger, Siri; Aas, Pal; Archives of Toxicology; vol. 80; nb. 8; (2006); p. 502 507, View in Reaxys

Merck 6391

Perusquia, Mercedes; Villalon, Carlos M.; Life Sciences; vol. 58; nb. 11; (1996); p. 913 - 926, View in Reaxys; Klein; Mascia; Harkness; Hadingham; Whiting; Harris; Journal of Pharmacology and Experimental Therapeutics; vol. 274; nb. 3; (1995); p. 1484 - 1492, View in Reaxys; Dirig; Yaksh; Journal of Pharmacology and Experimental Therapeutics; vol. 275; nb. 1; (1995); p. 219 - 227, View in Reaxys; Nolan; Gibson; Logan; British Journal of Pharmacology; vol.

121; nb. 2; (1997); p. 324 - 330, View in Reaxys; Zezula, Juergen; Slany, Astrid; Sieghart, Werner; European Journal of Pharmacology; vol. 301; nb. 1-3; (1996); p. 207 - 214, View in Reaxys; Raiteri, Maurizio; Bonanno, Giambattista; Paudice, Paolo; Cavazzani, Paolo; Schmid, Giovanna; Journal of Pharmacology and Experimental Therapeutics; vol.

278; nb. 2; (1996); p. 747 - 751, View in Reaxys; Nadeson; Guo; Porter; Gent; Goodchild; Journal of Pharmacology and Experimental Therapeutics; vol. 278; nb. 2; (1996); p. 620 - 626, View in Reaxys; Feuerstein; Gleichauf; Peckys; Landwehrmeyer; Scheremet; Jackisch; Naunyn-Schmiedeberg's Archives of Pharmacology; vol. 354; nb. 5; (1996); p.

586 - 592, View in Reaxys; Huh, Kyung-Hye; Endo, Shuichi; Olsen, Richard W.; European Journal of Pharmacology; vol. 310; nb. 2-3; (1996); p. 225 - 233, View in Reaxys; Kinoshita, Kiyoshi; Watanabe, Yumi; Yamamura, Michio; Matsuoka, Yuzo; European Journal of Pharmacology; vol. 343; nb. 2-3; (1998); p. 129 - 133, View in Reaxys; Zhang, Zhang-Jin; Trivedi, Bakula L.; De Paulis, Tomas; Schmidt, Dennis E.; Hewlett, William A.; Naunyn-Schmiedeberg's Archives of Pharmacology; vol. 365; nb. 2; (2002); p. 123 - 132, View in Reaxys

Mus

Kilbinger; Ginap; Erbelding; Naunyn-Schmiedeberg's Archives of Pharmacology; vol. 359; nb. 6; (1999); p. 500 504, View in Reaxys; Nobre, Manoel Jorge; Borelli, Karina Genaro; Brandao, Marcus L.; Life Sciences; vol. 71; nb. 25; (2002); p. 2961 - 2972, View in Reaxys

Merck 6336

Yoon, In-Seup; Kim, Hack-Seang; Hong, Jin-Tae; Lee, Myuhg-Koo; Oh, Ki-Wan; Pharmacology; vol. 65; nb. 4; (2002); p. 204 - 209, View in Reaxys

M (Merck: 6391)

Akhondzadeh; Stone; Life Sciences; vol. 58; nb. 13; (1996); p. 1023 - 1030, View in Reaxys

Merck 13 6336

Nakayama, Takahiro; Hashimoto, Tadatoshi; Nagai, Yasuo; European Journal of Pharmacology; vol. 316; nb. 2-3; (1996); p. 157 - 164, View in Reaxys

MERCK 6391

Hauser, Charlotte A. E.; Chesnoy-Marchais, Dominique; Robel, Paul; Baulieu, Etienne E.; European Journal of Pharmacology, Molecular Pharmacology Section; vol. 289; nb. 2; (1995); p. 249 - 258, View in Reaxys

II

TAKEMOTO; NAKAJIMA; YOKOBE; Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan; vol. 84; (1964); p. 1232 - 1233, View in Reaxys

Patent-Specific Data (2) Prophetic ComLocation in Patent pound

References

prophetic product

Page/page column 9

Patent; Amit Banerjee; US2006/35890; (2006); (A1) English, View in Reaxys

Claim

Patent; AJINOMOTO CO., INC.; US2001/51618; (2001); (A1) English, View in Reaxys; Patent; Mylari, Banavara L.; US2002/77319; (2002); (A1) English, View in Reaxys; Patent; Fractal Laboratories, Inc.; US5248678; (1993); (A) English, View in Reaxys; Patent; The Regents of the University of California; US6350769; (2002); (B1) English, View in Reaxys; Patent; AJINOMOTO CO., INC.; US2003/18027; (2003); (A1) English, View in Reaxys; Patent; Minuk, Gerald; US2002/156016; (2002); (A1) English, View in Reaxys; Patent; Mylari, Banavara L.; US2002/91128; (2002); (A1) English, View in Reaxys; Patent; Rhone Poulenc, Inc.; US5854002; (1998); (A) English, View in Reaxys

Copyright © 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

2/94

2018-08-03 20:32:29

Druglikeness (1) 1 of 1

LogP

-1.096

H Bond Donors

2

H Bond Acceptors

2

Rotatable Bonds

1

TPSA

64.35

Lipinski Number

4

Veber Number

2

Derivative (1) Comment (Derivative) HCl: F: 160grad(Zers.)

References Patent; Geigy; FR1427775; (1966); Chem.Abstr.; vol. 66; nb. 2554k; (1967), View in Reaxys

Melting Point (1) 1 of 1

Melting Point [°C]

177 - 178

TAKEMOTO; NAKAJIMA; YOKOBE; Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan; vol. 84; (1964); p.

1232 - 1233, View in Reaxys Use (92) Use Pattern

Location

References

GABAA receptor positive allosteric modulator (PAM) in all uses

Page/Page column title page; 47; 48; 49; 50; 51; 52; 53; 54; 56; 57

Patent; THE REGENTS OF THE UNIVERSITY OF CALIFORNIA; KAUFMAN, Daniel L.; TIAN, Jide; (76 pag.); WO2017/66240; (2017); (A1) English, View in Reaxys

Pharmaceuticals

Page/Page column title page; 47; 48; 49; 50; 51; 52; 53; 54; 56; 57

Patent; THE REGENTS OF THE UNIVERSITY OF CALIFORNIA; KAUFMAN, Daniel L.; TIAN, Jide; (76 pag.); WO2017/66240; (2017); (A1) English, View in Reaxys

absence seizures

Page/Page column title page; 47; 48; 49; 50; 51; 52; 53; 54; 56; 57

Patent; THE REGENTS OF THE UNIVERSITY OF CALIFORNIA; KAUFMAN, Daniel L.; TIAN, Jide; (76 pag.); WO2017/66240; (2017); (A1) English, View in Reaxys

agoraphobia

Page/Page column title page; 47; 48; 49; 50; 51; 52; 53; 54; 56; 57

Patent; THE REGENTS OF THE UNIVERSITY OF CALIFORNIA; KAUFMAN, Daniel L.; TIAN, Jide; (76 pag.); WO2017/66240; (2017); (A1) English, View in Reaxys

anesthesia

Page/Page column title page; 47; 48; 49; 50; 51; 52; 53; 54; 56; 57

Patent; THE REGENTS OF THE UNIVERSITY OF CALIFORNIA; KAUFMAN, Daniel L.; TIAN, Jide; (76 pag.); WO2017/66240; (2017); (A1) English, View in Reaxys

anxiety caused by depression

Page/Page column title page; 47; 48; 49; 50; 51; 52; 53; 54; 56; 57

Patent; THE REGENTS OF THE UNIVERSITY OF CALIFORNIA; KAUFMAN, Daniel L.; TIAN, Jide; (76 pag.); WO2017/66240; (2017); (A1) English, View in Reaxys

anxiety disorder

Page/Page column title page; 47; 48; 49; 50; 51; 52; 53; 54; 56; 57

Patent; THE REGENTS OF THE UNIVERSITY OF CALIFORNIA; KAUFMAN, Daniel L.; TIAN, Jide; (76 pag.); WO2017/66240; (2017); (A1) English, View in Reaxys

catamenial epilepsy.

Page/Page column title page; 47; 48; 49; 50; 51; 52; 53; 54; 56; 57

Patent; THE REGENTS OF THE UNIVERSITY OF CALIFORNIA; KAUFMAN, Daniel L.; TIAN, Jide; (76 pag.); WO2017/66240; (2017); (A1) English, View in Reaxys

increases beta cell replication in islets after islet transplantation

Page/Page column title page; 47; 48; 49; 50; 51; 52; 53; 54; 56; 57

Patent; THE REGENTS OF THE UNIVERSITY OF CALIFORNIA; KAUFMAN, Daniel L.; TIAN, Jide; (76 pag.); WO2017/66240; (2017); (A1) English, View in Reaxys

Copyright © 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

3/94

2018-08-03 20:32:29

increases insulin content

Page/Page column title page; 47; 48; 49; 50; 51; 52; 53; 54; 56; 57

Patent; THE REGENTS OF THE UNIVERSITY OF CALIFORNIA; KAUFMAN, Daniel L.; TIAN, Jide; (76 pag.); WO2017/66240; (2017); (A1) English, View in Reaxys

increasing mass of beta cells

Page/Page column title page; 47; 48; 49; 50; 51; 52; 53; 54; 56; 57

Patent; THE REGENTS OF THE UNIVERSITY OF CALIFORNIA; KAUFMAN, Daniel L.; TIAN, Jide; (76 pag.); WO2017/66240; (2017); (A1) English, View in Reaxys

lennox-gastaut syn- Page/Page column drome title page; 47; 48; 49; 50; 51; 52; 53; 54; 56; 57

Patent; THE REGENTS OF THE UNIVERSITY OF CALIFORNIA; KAUFMAN, Daniel L.; TIAN, Jide; (76 pag.); WO2017/66240; (2017); (A1) English, View in Reaxys

neuropathic pain

Page/Page column title page; 47; 48; 49; 50; 51; 52; 53; 54; 56; 57

Patent; THE REGENTS OF THE UNIVERSITY OF CALIFORNIA; KAUFMAN, Daniel L.; TIAN, Jide; (76 pag.); WO2017/66240; (2017); (A1) English, View in Reaxys

panic disorder

Page/Page column title page; 47; 48; 49; 50; 51; 52; 53; 54; 56; 57

Patent; THE REGENTS OF THE UNIVERSITY OF CALIFORNIA; KAUFMAN, Daniel L.; TIAN, Jide; (76 pag.); WO2017/66240; (2017); (A1) English, View in Reaxys

producing drowsiness

Page/Page column title page; 47; 48; 49; 50; 51; 52; 53; 54; 56; 57

Patent; THE REGENTS OF THE UNIVERSITY OF CALIFORNIA; KAUFMAN, Daniel L.; TIAN, Jide; (76 pag.); WO2017/66240; (2017); (A1) English, View in Reaxys

promoting function of beta cells

Page/Page column title page; 47; 48; 49; 50; 51; 52; 53; 54; 56; 57

Patent; THE REGENTS OF THE UNIVERSITY OF CALIFORNIA; KAUFMAN, Daniel L.; TIAN, Jide; (76 pag.); WO2017/66240; (2017); (A1) English, View in Reaxys

reduce 13-cell loss due to hypoxia and stress

Page/Page column title page; 47; 48; 49; 50; 51; 52; 53; 54; 56; 57

Patent; THE REGENTS OF THE UNIVERSITY OF CALIFORNIA; KAUFMAN, Daniel L.; TIAN, Jide; (76 pag.); WO2017/66240; (2017); (A1) English, View in Reaxys

replication of beta cells

Page/Page column title page; 47; 48; 49; 50; 51; 52; 53; 54; 56; 57

Patent; THE REGENTS OF THE UNIVERSITY OF CALIFORNIA; KAUFMAN, Daniel L.; TIAN, Jide; (76 pag.); WO2017/66240; (2017); (A1) English, View in Reaxys

seizure disorders

Page/Page column title page; 47; 48; 49; 50; 51; 52; 53; 54; 56; 57

Patent; THE REGENTS OF THE UNIVERSITY OF CALIFORNIA; KAUFMAN, Daniel L.; TIAN, Jide; (76 pag.); WO2017/66240; (2017); (A1) English, View in Reaxys

spinal cord injury

Page/Page column title page; 47; 48; 49; 50; 51; 52; 53; 54; 56; 57

Patent; THE REGENTS OF THE UNIVERSITY OF CALIFORNIA; KAUFMAN, Daniel L.; TIAN, Jide; (76 pag.); WO2017/66240; (2017); (A1) English, View in Reaxys

survival of beta cells

Page/Page column title page; 47; 48; 49; 50; 51; 52; 53; 54; 56; 57

Patent; THE REGENTS OF THE UNIVERSITY OF CALIFORNIA; KAUFMAN, Daniel L.; TIAN, Jide; (76 pag.); WO2017/66240; (2017); (A1) English, View in Reaxys

sleep disorders

Patent; SEPRACOR INC.; WO2005/63297; (2005); (A2) English, View in Reaxys; Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

GABA agonist

Patent; Alkermes, Inc.; US2007/99947; (2007); (A1) English, View in Reaxys

composition for treatment of brain reward system disorders comprising opioid antagonist and agent effective to eliminate symptoms of brain re-

Patent; Alkermes, Inc.; US2007/99947; (2007); (A1) English, View in Reaxys

Copyright © 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

4/94

2018-08-03 20:32:29

ward system disorders active ingredient

Patent; Alkermes, Inc.; US2007/99947; (2007); (A1) English, View in Reaxys

anxiety

Patent; Alkermes, Inc.; US2007/99947; (2007); (A1) English, View in Reaxys

nausea

Patent; Alkermes, Inc.; US2007/99947; (2007); (A1) English, View in Reaxys

excitability

Patent; Alkermes, Inc.; US2007/99947; (2007); (A1) English, View in Reaxys

craving

Patent; Alkermes, Inc.; US2007/99947; (2007); (A1) English, View in Reaxys

irritability

Patent; Alkermes, Inc.; US2007/99947; (2007); (A1) English, View in Reaxys

impulsivity

Patent; Alkermes, Inc.; US2007/99947; (2007); (A1) English, View in Reaxys

anger

Patent; Alkermes, Inc.; US2007/99947; (2007); (A1) English, View in Reaxys

rage

Patent; Alkermes, Inc.; US2007/99947; (2007); (A1) English, View in Reaxys

pathological gambling

Patent; Alkermes, Inc.; US2007/99947; (2007); (A1) English, View in Reaxys

compulsive alcohol consumption

Patent; Alkermes, Inc.; US2007/99947; (2007); (A1) English, View in Reaxys

compulsive overeating

Patent; Alkermes, Inc.; US2007/99947; (2007); (A1) English, View in Reaxys

obesity

Patent; Alkermes, Inc.; US2007/99947; (2007); (A1) English, View in Reaxys; Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

compulsive smoking

Patent; Alkermes, Inc.; US2007/99947; (2007); (A1) English, View in Reaxys

drug addiction

Patent; Alkermes, Inc.; US2007/99947; (2007); (A1) English, View in Reaxys

neuropsychiatric disorder

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

Addiction

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

Symptoms during menopause

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

Symptoms associated with the menstrual cycle

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

Anxiety

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

Depression

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

Schizophrenia

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

Insomnia

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

disruptive behavior disorders

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

attention deficit disorders

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

Copyright © 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

5/94

2018-08-03 20:32:29

attention deficit and hyperactivity disorder (ADHD)

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

pre-menstrual syndrome (PMS)

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

panic disorder

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

obsessive compulsive disorder (OCD)

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

post-traumatic stress disorder (PTSD)

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

social anxiety disorder

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

dysthymia

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

bipolar depression

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

depression associated with medical conditions

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

depression associated with substance abuse

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

Attention-defficit disorder (ADD)

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

Traumatic stress disorder (PTSD)

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

Eating disorder

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

Sleep disorder

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

Disruptive behavior disorder

Patent; BOARD OF SUPERVISORS OF LOUISIANA STATE UNIVERSITY and AGRICULTURAL and MECHANICAL COLLEGE; WO2007/56618; (2007); (A1) English, View in Reaxys

insomnia

Patent; Alkermes, Inc.; US2007/99947; (2007); (A1) English, View in Reaxys; Patent; SEPRACOR INC.; WO2005/63248; (2005); (A1) English, View in Reaxys

An agent for improving mobility and general health of senior companion animals

Patent; Inagawa, Kentaro; Bannai, Makoto; Seki, Shinobu; Kogure, Norio; US2005/106220; (2005); (A1) English, View in Reaxys

pet food additive

Patent; Inagawa, Kentaro; Bannai, Makoto; Seki, Shinobu; Kogure, Norio; US2005/106220; (2005); (A1) English, View in Reaxys

pet food

Patent; Inagawa, Kentaro; Bannai, Makoto; Seki, Shinobu; Kogure, Norio; US2005/106220; (2005); (A1) English, View in Reaxys

Copyright © 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

6/94

2018-08-03 20:32:29

difficulty breathing

Patent; Inagawa, Kentaro; Bannai, Makoto; Seki, Shinobu; Kogure, Norio; US2005/106220; (2005); (A1) English, View in Reaxys

forced or labored breathing

Patent; Inagawa, Kentaro; Bannai, Makoto; Seki, Shinobu; Kogure, Norio; US2005/106220; (2005); (A1) English, View in Reaxys

leg trembling

Patent; Inagawa, Kentaro; Bannai, Makoto; Seki, Shinobu; Kogure, Norio; US2005/106220; (2005); (A1) English, View in Reaxys

difficulty walking

Patent; Inagawa, Kentaro; Bannai, Makoto; Seki, Shinobu; Kogure, Norio; US2005/106220; (2005); (A1) English, View in Reaxys

decreased reaction times

Patent; Inagawa, Kentaro; Bannai, Makoto; Seki, Shinobu; Kogure, Norio; US2005/106220; (2005); (A1) English, View in Reaxys

decreased excretion

Patent; Inagawa, Kentaro; Bannai, Makoto; Seki, Shinobu; Kogure, Norio; US2005/106220; (2005); (A1) English, View in Reaxys

failure of one or more of the five senses

Patent; Inagawa, Kentaro; Bannai, Makoto; Seki, Shinobu; Kogure, Norio; US2005/106220; (2005); (A1) English, View in Reaxys

loss of directional sense

Patent; Inagawa, Kentaro; Bannai, Makoto; Seki, Shinobu; Kogure, Norio; US2005/106220; (2005); (A1) English, View in Reaxys

inactivity

Patent; Inagawa, Kentaro; Bannai, Makoto; Seki, Shinobu; Kogure, Norio; US2005/106220; (2005); (A1) English, View in Reaxys

Agonist to the Clchannel-involving bicuculline sensitive receptor

Patent; SHISEIDO COMPANY LIMITED; EP1550459; (2005); (A1) English, View in Reaxys

Parakeratosis inhibitor

Patent; SHISEIDO COMPANY LIMITED; EP1550459; (2005); (A1) English, View in Reaxys

Parakeratosis inhibitory skin preparation for external use

Patent; SHISEIDO COMPANY LIMITED; EP1550459; (2005); (A1) English, View in Reaxys

Pore-shrinking agent

Patent; SHISEIDO COMPANY LIMITED; EP1550459; (2005); (A1) English, View in Reaxys

Pore-shrinking skin preparation for external use

Patent; SHISEIDO COMPANY LIMITED; EP1550459; (2005); (A1) English, View in Reaxys

sedative agent

Patent; SEPRACOR INC.; WO2005/63297; (2005); (A2) English, View in Reaxys

sleep abnormality or insomnia

Patent; SEPRACOR INC.; WO2005/63297; (2005); (A2) English, View in Reaxys

Transient insomnia

Patent; SEPRACOR INC.; WO2005/63297; (2005); (A2) English, View in Reaxys

Short-term insomnia

Patent; SEPRACOR INC.; WO2005/63297; (2005); (A2) English, View in Reaxys

Chronic insomnia

Patent; SEPRACOR INC.; WO2005/63297; (2005); (A2) English, View in Reaxys

Improving sleep quality

Patent; SEPRACOR INC.; WO2005/63248; (2005); (A1) English, View in Reaxys

transient insomnia

Patent; SEPRACOR INC.; WO2005/63248; (2005); (A1) English, View in Reaxys

short-term insomnia

Patent; SEPRACOR INC.; WO2005/63248; (2005); (A1) English, View in Reaxys

chronic insomnia

Patent; SEPRACOR INC.; WO2005/63248; (2005); (A1) English, View in Reaxys

Sedative-gypnotic agent

Patent; SEPRACOR INC.; WO2005/63248; (2005); (A1) English, View in Reaxys

Medchem (189) 1 of 189

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Copyright © 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

7/94

2018-08-03 20:32:29

2 of 189

3 of 189

Bioassay Name

Binding

Biological Species/NCBI ID

rat

Organs/Tissues

forebrain

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-Muscimol

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Quantitative value

7

Measurement pX

8.15

Concomitants: Compound RN

906818

Concomitants: Compound name

GABA

Concomitants: Compound role

NSB

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Biological Species/NCBI ID

rat

Organs/Tissues

forebrain

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-Muscimol

Measurement Parameter

Bmax

Unit

pmol/g tissue

Quantitative value

41

Concomitants: Compound RN

906818

Concomitants: Compound name

GABA

Concomitants: Compound role

NSB

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Biological Species/NCBI ID

rat

Organs/Tissues

forebrain

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-Muscimol

Measurement Parameter

Kd (dissociation constant)

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

8/94

2018-08-03 20:32:29

4 of 189

5 of 189

Unit

nM

Quantitative value

8.2

Measurement pX

8.09

Concomitants: Compound RN

906818; 548872

Concomitants: Compound name

GABA; Etazolate

Concomitants: Compound role

NSB; COM

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Biological Species/NCBI ID

rat

Organs/Tissues

forebrain

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-Muscimol

Measurement Parameter

Bmax

Unit

pmol/g tissue

Quantitative value

193

Concomitants: Compound RN

906818; 548872

Concomitants: Compound name

GABA; Etazolate

Concomitants: Compound role

NSB; COM

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Biological Species/NCBI ID

Chbb:THOM rat

Organs/Tissues

cerebellum

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Measurement Parameter

Kd (dissociation constant)

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

9/94

2018-08-03 20:32:29

6 of 189

7 of 189

Unit

nM

Measurement Object

HIGH

Quantitative value

2.11

Measurement pX

8.68

Concomitants: Compound RN

754371; 606417

Concomitants: Compound name

Diazepam; Muscimol

Concomitants: Compound role

COM; NSB

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Biological Species/NCBI ID

Chbb:THOM rat

Organs/Tissues

cerebellum

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Measurement Parameter

Bmax

Unit

pmol/g

Measurement Object

HIGH

Quantitative value

1.74

Concomitants: Compound RN

754371; 606417

Concomitants: Compound name

Diazepam; Muscimol

Concomitants: Compound role

COM; NSB

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

10/94

2018-08-03 20:32:29

8 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Biological Species/NCBI ID

Chbb:THOM rat

Organs/Tissues

cerebellum

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Measurement Object

HIGH

Quantitative value

2.11

Measurement pX

8.68

Concomitants: Compound RN

606417; 839277

Concomitants: Compound name

Muscimol; Brotizolam

Concomitants: Compound role

NSB; COM

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Biological Species/NCBI ID

Chbb:THOM rat

Organs/Tissues

cerebellum

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Measurement Parameter

Bmax

Unit

pmol/g

Measurement Object

HIGH

Quantitative value

1.74

Concomitants: Compound RN

606417; 839277

Concomitants: Compound name

Muscimol; Brotizolam

Concomitants: Compound role

NSB; COM

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

11/94

2018-08-03 20:32:29

9 of 189

10 of 189

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Biological Species/NCBI ID

Chbb:THOM rat

Organs/Tissues

cerebellum

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Measurement Object

LOW

Quantitative value

11.32

Measurement pX

7.95

Concomitants: Compound RN

754371; 606417

Concomitants: Compound name

Diazepam; Muscimol

Concomitants: Compound role

COM; NSB

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Biological Species/NCBI ID

Chbb:THOM rat

Organs/Tissues

cerebellum

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

12/94

2018-08-03 20:32:29

11 of 189

12 of 189

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Measurement Object

LOW

Quantitative value

11.32

Measurement pX

7.95

Concomitants: Compound RN

606417; 839277

Concomitants: Compound name

Muscimol; Brotizolam

Concomitants: Compound role

NSB; COM

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Biological Species/NCBI ID

Chbb:THOM rat

Organs/Tissues

cerebellum

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Measurement Parameter

Bmax

Unit

pmol/g

Quantitative value

2.7

Concomitants: Compound RN

754371; 606417

Concomitants: Compound name

Diazepam; Muscimol

Concomitants: Compound role

COM; NSB

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

13/94

2018-08-03 20:32:29

13 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Biological Species/NCBI ID

Chbb:THOM rat

Organs/Tissues

cerebellum

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Measurement Parameter

Bmax

Unit

pmol/g

Quantitative value

2.7

Concomitants: Compound RN

606417; 839277

Concomitants: Compound name

Muscimol; Brotizolam

Concomitants: Compound role

NSB; COM

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Biological Species/NCBI ID

Chbb:THOM rat

Organs/Tissues

cerebellum

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Measurement Object

HIGH

Quantitative value

2.42

Deviation

0.680000

Measurement pX

8.62

Concomitants: Compound RN

754371; 606417

Concomitants: Compound name

Diazepam; Muscimol

Concomitants: Compound role

COM; NSB

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

14/94

2018-08-03 20:32:29

14 of 189

15 of 189

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Biological Species/NCBI ID

Chbb:THOM rat

Organs/Tissues

cerebellum

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Measurement Parameter

Bmax

Unit

pmol/g

Measurement Object

HIGH

Quantitative value

1.84

Deviation

0.380000

Concomitants: Compound RN

754371; 606417

Concomitants: Compound name

Diazepam; Muscimol

Concomitants: Compound role

COM; NSB

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Biological Species/NCBI ID

Chbb:THOM rat

Organs/Tissues

cerebellum

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

15/94

2018-08-03 20:32:29

16 of 189

17 of 189

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Measurement Object

HIGH

Quantitative value

2.97

Deviation

1.18000

Measurement pX

8.53

Concomitants: Compound RN

606417; 839277

Concomitants: Compound name

Muscimol; Brotizolam

Concomitants: Compound role

NSB; COM

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Biological Species/NCBI ID

Chbb:THOM rat

Organs/Tissues

cerebellum

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Measurement Parameter

Bmax

Unit

pmol/g

Measurement Object

HIGH

Quantitative value

1.78

Deviation

0.250000

Concomitants: Compound RN

606417; 839277

Concomitants: Compound name

Muscimol; Brotizolam

Concomitants: Compound role

NSB; COM

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

16/94

2018-08-03 20:32:29

18 of 189

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Biological Species/NCBI ID

Chbb:THOM rat

Organs/Tissues

cerebellum

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Measurement Object

LOW

Quantitative value

7.33

Deviation

3.24000

Measurement pX

8.13

Concomitants: Compound RN

754371; 606417

Concomitants: Compound name

Diazepam; Muscimol

Concomitants: Compound role

COM; NSB

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Biological Species/NCBI ID

Chbb:THOM rat

Organs/Tissues

cerebellum

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Measurement Object

LOW

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

17/94

2018-08-03 20:32:29

19 of 189

20 of 189

Quantitative value

7.53

Deviation

2.44000

Measurement pX

8.12

Concomitants: Compound RN

606417; 839277

Concomitants: Compound name

Muscimol; Brotizolam

Concomitants: Compound role

NSB; COM

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Biological Species/NCBI ID

Chbb:THOM rat

Organs/Tissues

cerebellum

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Measurement Parameter

Bmax

Unit

pmol/g

Quantitative value

2.74

Deviation

0.460000

Concomitants: Compound RN

754371; 606417

Concomitants: Compound name

Diazepam; Muscimol

Concomitants: Compound role

COM; NSB

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

18/94

2018-08-03 20:32:29

21 of 189

Bioassay Name

Binding

Biological Species/NCBI ID

Chbb:THOM rat

Organs/Tissues

cerebellum

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Measurement Parameter

Bmax

Unit

pmol/g

Quantitative value

2.64

Deviation

0.850000

Concomitants: Compound RN

606417; 839277

Concomitants: Compound name

Muscimol; Brotizolam

Concomitants: Compound role

NSB; COM

Target Name

Gamma-aminobutyric acid receptor subunit gamma-2 [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat];+Gamma-aminobutyric acid receptor subunit alpha-1 [rat]

Target Synonyms

gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p18508 + p63138 + p62813

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit gamma-2 [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit gamma-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Cells/Cell Lines

tsA201 cell line

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H)-muscimol

Substance Dose

0 nM

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Measurement Object

HIGH

Quantitative value

8.9

Deviation

0.500000

Measurement pX

8.05

Concomitants: Compound RN

754371

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

19/94

2018-08-03 20:32:29

22 of 189

23 of 189

Concomitants: Compound name

Diazepam

Concomitants: Compound role

NSB

Target Name

Gamma-aminobutyric acid receptor subunit gamma-2 [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat];+Gamma-aminobutyric acid receptor subunit alpha-1 [rat]

Target Synonyms

gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p18508 + p63138 + p62813

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit gamma-2 [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit gamma-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Cells/Cell Lines

tsA201 cell line

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H)-muscimol

Substance Dose

0 nM

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Measurement Object

LOW

Quantitative value

429.1

Deviation

40.3000

Measurement pX

6.37

Concomitants: Compound RN

754371

Concomitants: Compound name

Diazepam

Concomitants: Compound role

NSB

Target Name

Gamma-aminobutyric acid receptor subunit beta-2 [rat];+Gamma-aminobutyric acid receptor subunit gamma-2 [rat];+Gamma-aminobutyric acid receptor subunit alpha-1 [rat]

Target Synonyms

gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p18508 + p62813

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit gamma-2 [rat]

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

20/94

2018-08-03 20:32:29

24 of 189

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Mutated:Substitution,1,Tyrosine,86,Phenylalanine + Gamma-aminobutyric acid receptor subunit gamma-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Cells/Cell Lines

tsA201 cell line

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H)-muscimol

Substance Dose

0 nM

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Quantitative value

57.6

Deviation

11.1000

Statistical sgnificance (pvalue)

0.01

Measurement pX

7.24

Concomitants: Compound RN

754371

Concomitants: Compound name

Diazepam

Concomitants: Compound role

NSB

Target Name

Gamma-aminobutyric acid receptor subunit beta-2 [rat];+Gamma-aminobutyric acid receptor subunit gamma-2 [rat];+Gamma-aminobutyric acid receptor subunit alpha-1 [rat]

Target Synonyms

gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p18508 + p62813

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit gamma-2 [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Mutated:Substitution,1,Tyrosine,86,Serine + Gamma-aminobutyric acid receptor subunit gamma-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Cells/Cell Lines

tsA201 cell line

Cell Fraction

Membrane

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

21/94

2018-08-03 20:32:29

25 of 189

Substance RN

774694View in Reaxys

Substance Name

[3H)-muscimol

Substance Dose

0 nM

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Quantitative value

316.9

Deviation

28.4000

Measurement pX

6.5

Concomitants: Compound RN

754371

Concomitants: Compound name

Diazepam

Concomitants: Compound role

NSB

Target Name

Gamma-aminobutyric acid receptor subunit beta-2 [rat];+Gamma-aminobutyric acid receptor subunit gamma-2 [rat];+Gamma-aminobutyric acid receptor subunit alpha-1 [rat]

Target Synonyms

gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p18508 + p62813

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit gamma-2 [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Mutated:Substitution,1,Tyrosine,98,Phenylalanine + Gamma-aminobutyric acid receptor subunit gamma-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Cells/Cell Lines

tsA201 cell line

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H)-muscimol

Substance Dose

0 nM

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Measurement Object

HIGH

Quantitative value

7

Deviation

5.20000

Measurement pX

8.15

Concomitants: Compound RN

754371

Concomitants: Compound name

Diazepam

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

22/94

2018-08-03 20:32:29

26 of 189

27 of 189

Concomitants: Compound role

NSB

Target Name

Gamma-aminobutyric acid receptor subunit beta-2 [rat];+Gamma-aminobutyric acid receptor subunit gamma-2 [rat];+Gamma-aminobutyric acid receptor subunit alpha-1 [rat]

Target Synonyms

gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p18508 + p62813

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit gamma-2 [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Mutated:Substitution,1,Tyrosine,98,Phenylalanine + Gamma-aminobutyric acid receptor subunit gamma-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Cells/Cell Lines

tsA201 cell line

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H)-muscimol

Substance Dose

0 nM

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Measurement Object

LOW

Quantitative value

360

Deviation

140

Measurement pX

6.44

Concomitants: Compound RN

754371

Concomitants: Compound name

Diazepam

Concomitants: Compound role

NSB

Target Name

Gamma-aminobutyric acid receptor subunit beta-2 [rat];+Gamma-aminobutyric acid receptor subunit gamma-2 [rat];+Gamma-aminobutyric acid receptor subunit alpha-1 [rat]

Target Synonyms

gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p18508 + p62813

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit gamma-2 [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Mutated:Substitution,1,Tyrosine,98,Serine + Gamma-aminobutyric acid receptor subunit gamma-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Wild

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

23/94

2018-08-03 20:32:29

28 of 189

Target Species (Bioactivity)

rat

Target Transfection

Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Cells/Cell Lines

tsA201 cell line

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H)-muscimol

Substance Dose

0 nM

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Measurement Object

HIGH

Quantitative value

10.6

Deviation

5.40000

Measurement pX

7.97

Concomitants: Compound RN

754371

Concomitants: Compound name

Diazepam

Concomitants: Compound role

NSB

Target Name

Gamma-aminobutyric acid receptor subunit beta-2 [rat];+Gamma-aminobutyric acid receptor subunit gamma-2 [rat];+Gamma-aminobutyric acid receptor subunit alpha-1 [rat]

Target Synonyms

gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p18508 + p62813

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit gamma-2 [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Mutated:Substitution,1,Tyrosine,98,Serine + Gamma-aminobutyric acid receptor subunit gamma-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Cells/Cell Lines

tsA201 cell line

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H)-muscimol

Substance Dose

0 nM

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

24/94

2018-08-03 20:32:29

29 of 189

30 of 189

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Measurement Object

LOW

Quantitative value

1242

Deviation

741

Measurement pX

5.91

Concomitants: Compound RN

754371

Concomitants: Compound name

Diazepam

Concomitants: Compound role

NSB

Target Name

Gamma-aminobutyric acid receptor subunit alpha-6 [rat];+Gamma-aminobutyric acid receptor subunit gamma-2 [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat]

Target Synonyms

gaba(a) receptor subunit alpha-6; gabra-6; gabra6; gamma-aminobutyric acid receptor subunit alpha-6 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2

Target Uniprot ID

p30191 + p18508 + p63138

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-6 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit gamma-2 [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit alpha-6 [rat]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild

Target Species (Bioactivity)

rat

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Cells/Cell Lines

Sf9 cell line

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Quantitative value

4.8

Deviation

0.800000

Measurement pX

8.32

Target Name

Gamma-aminobutyric acid receptor subunit alpha-6 [rat];+Gamma-aminobutyric acid receptor subunit gamma-2 [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat]

Target Synonyms

gaba(a) receptor subunit alpha-6; gabra-6; gabra6; gamma-aminobutyric acid receptor subunit alpha-6 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2

Target Uniprot ID

p30191 + p18508 + p63138

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-6 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit gamma-2 [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit alpha-6 [rat]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

25/94

2018-08-03 20:32:29

31 of 189

32 of 189

Target Species (Bioactivity)

rat

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Cells/Cell Lines

Sf9 cell line

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Quantitative value

4.8

Measurement pX

8.32

Target Name

Gamma-aminobutyric acid receptor subunit alpha-6 [rat];+Gamma-aminobutyric acid receptor subunit gamma-2 [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat]

Target Synonyms

gaba(a) receptor subunit alpha-6; gabra-6; gabra6; gamma-aminobutyric acid receptor subunit alpha-6 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2

Target Uniprot ID

p30191 + p18508 + p63138

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-6 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit gamma-2 [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit alpha-6 [rat]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild

Target Species (Bioactivity)

rat

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Cells/Cell Lines

Sf9 cell line

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Quantitative value

4.8

Deviation

0.300000

Measurement pX

8.32

Target Name

ionotropic gamma aminobutyric acid a receptor [rat];+Gamma-aminobutyric acid receptor subunit gamma-2 [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2

Target Uniprot ID

p18508 + p63138

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit gamma-2 [rat]; ionotropic gamma aminobutyric acid a receptor [rat]

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

26/94

2018-08-03 20:32:29

33 of 189

34 of 189

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild

Target Species (Bioactivity)

rat

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Cells/Cell Lines

Sf9 cell line

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Quantitative value

4.8

Measurement pX

8.32

Target Name

Gamma-aminobutyric acid receptor subunit alpha-6 [rat];+Gamma-aminobutyric acid receptor subunit gamma-2 [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat]

Target Synonyms

gaba(a) receptor subunit alpha-6; gabra-6; gabra6; gamma-aminobutyric acid receptor subunit alpha-6 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2

Target Uniprot ID

p18508 + p63138

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-6 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit gamma-2 [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit alpha-6 [rat]:Mutated:Substitution,1,Arginine,119,Histidine + Gamma-aminobutyric acid receptor subunit gamma-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild

Target Species (Bioactivity)

rat

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Cells/Cell Lines

Sf9 cell line

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Quantitative value

7

Measurement pX

8.15

Target Name

Gamma-aminobutyric acid receptor subunit alpha-6 [rat];+Gamma-aminobutyric acid receptor subunit gamma-2 [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat]

Target Synonyms

gaba(a) receptor subunit alpha-6; gabra-6; gabra6; gamma-aminobutyric acid receptor subunit alpha-6 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2

Target Uniprot ID

p18508 + p63138

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

27/94

2018-08-03 20:32:29

35 of 189

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-6 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit gamma-2 [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit alpha-6 [rat]:Mutated:Substitution,1,Arginine,119,Histidine,Substitution,2,Histidine,140,Glutamic acid,Substitution,3,Asparagine,141,Aspartic acid + Gamma-aminobutyric acid receptor subunit gamma-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild

Target Species (Bioactivity)

rat

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Cells/Cell Lines

Sf9 cell line

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Quantitative value

4.2

Deviation

0.800000

Measurement pX

8.38

Target Name

Gamma-aminobutyric acid receptor subunit alpha-6 [rat];+Gamma-aminobutyric acid receptor subunit gamma-2 [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat]

Target Synonyms

gaba(a) receptor subunit alpha-6; gabra-6; gabra6; gamma-aminobutyric acid receptor subunit alpha-6 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2

Target Uniprot ID

p18508 + p63138

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-6 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit gamma-2 [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit alpha-6 [rat]:Mutated:Substitution,1,Arginine,119,Histidine,Substitution,2,Histidine,140,Glutamic acid,Substitution,3,Asparagine,141,Aspartic acid,Substitution,4,Valine,161,Glutamic acid,Substitution,5,Asparagine,162,Aspartic acid + Gamma-aminobutyric acid receptor subunit gamma-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild

Target Species (Bioactivity)

rat

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Cells/Cell Lines

Sf9 cell line

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Quantitative value

3

Measurement pX

8.52

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

28/94

2018-08-03 20:32:29

36 of 189

37 of 189

Target Name

ionotropic gamma aminobutyric acid a receptor [rat];+Gamma-aminobutyric acid receptor subunit gamma-2 [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2

Target Uniprot ID

p18508 + p63138

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit gamma-2 [rat]; ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Chimera + Gamma-aminobutyric acid receptor subunit gamma-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild

Target Species (Bioactivity)

rat

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Cells/Cell Lines

Sf9 cell line

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Quantitative value

4.4

Deviation

2

Measurement pX

8.36

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Biological Species/NCBI ID

rat

Organs/Tissues

thalamus

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-Muscimol

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Quantitative value

16

Deviation

6

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

29/94

2018-08-03 20:32:29

38 of 189

39 of 189

Measurement pX

7.8

Concomitants: Compound RN

906818

Concomitants: Compound name

GABA

Concomitants: Compound role

NSB

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Biological Species/NCBI ID

rat

Organs/Tissues

thalamus

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-Muscimol

Substance Dose

40 nM

Measurement Parameter

Bmax

Unit

fmol/mg protein

Quantitative value

698

Deviation

98

Concomitants: Compound RN

906818

Concomitants: Compound name

GABA

Concomitants: Compound role

NSB

Target Name

Gamma-aminobutyric acid receptor subunit gamma-2 [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat];+Gamma-aminobutyric acid receptor subunit alpha-1 [rat]

Target Synonyms

gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p18508 + p63138 + p62813

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit gamma-2 [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit gamma-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Wild

Target Species (Bioactivity)

rat

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

30/94

2018-08-03 20:32:29

40 of 189

Target Transfection

Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Cells/Cell Lines

HEK293 cell line

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-Muscimol

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Quantitative value

6

Deviation

5

Measurement pX

8.22

Concomitants: Compound RN

606417

Concomitants: Compound name

Muscimol

Concomitants: Compound role

NSB

Target Name

Gamma-aminobutyric acid receptor subunit gamma-2 [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat];+Gamma-aminobutyric acid receptor subunit alpha-1 [rat]

Target Synonyms

gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p18508 + p63138 + p62813

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit gamma-2 [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit gamma-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Cells/Cell Lines

HEK293 cell line

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-Muscimol

Measurement Parameter

Bmax

Unit

pmol/mg protein

Quantitative value

1.6

Deviation

0.600000

Concomitants: Compound RN

606417

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

31/94

2018-08-03 20:32:29

41 of 189

42 of 189

Concomitants: Compound name

Muscimol

Concomitants: Compound role

NSB

Target Name

Gamma-aminobutyric acid receptor subunit alpha-1 [rat];+Gamma-aminobutyric acid receptor subunit gamma-2 [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat]

Target Synonyms

gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2

Target Uniprot ID

p18508 + p63138

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit gamma-2 [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Mutated:Substitution,1,Tyrosine,236,Glutamine + Gamma-aminobutyric acid receptor subunit gamma-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Cells/Cell Lines

HEK293 cell line

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-Muscimol

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Quantitative value

14

Deviation

6

Measurement pX

7.85

Concomitants: Compound RN

606417

Concomitants: Compound name

Muscimol

Concomitants: Compound role

NSB

Target Name

Gamma-aminobutyric acid receptor subunit alpha-1 [rat];+Gamma-aminobutyric acid receptor subunit gamma-2 [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat]

Target Synonyms

gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2

Target Uniprot ID

p18508 + p63138

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit gamma-2 [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Mutated:Substitution,1,Tyrosine,236,Glutamine + Gamma-aminobutyric acid receptor subunit gamma-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

32/94

2018-08-03 20:32:29

43 of 189

Target Species (Bioactivity)

rat

Target Transfection

Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Cells/Cell Lines

HEK293 cell line

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-Muscimol

Measurement Parameter

Bmax

Unit

pmol/mg protein

Quantitative value

0.74

Deviation

0.140000

Concomitants: Compound RN

606417

Concomitants: Compound name

Muscimol

Concomitants: Compound role

NSB

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Biological Species/NCBI ID

rat

Organs/Tissues

forebrain

Cells/Cell Lines

nerve cell

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-Muscimol

Substance Dose

2 nM

Measurement Parameter

Bmax

Unit

fmol/mg

Quantitative value

200

Deviation

27

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

33/94

2018-08-03 20:32:29

44 of 189

45 of 189

Concomitants: Compound RN

1982052; 759557

Concomitants: Compound name

Honokiol; Clonazepam

Concomitants: Compound role

COM; NSB

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Biological Species/NCBI ID

rat

Organs/Tissues

forebrain

Cells/Cell Lines

nerve cell

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-Muscimol

Substance Dose

2 nM

Measurement Parameter

Bmax

Unit

fmol/mg

Quantitative value

180

Deviation

15

Concomitants: Compound RN

1982224; 759557

Concomitants: Compound name

Magnolol; Clonazepam

Concomitants: Compound role

COM; NSB

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

34/94

2018-08-03 20:32:29

46 of 189

Biological Species/NCBI ID

rat

Organs/Tissues

forebrain

Cells/Cell Lines

nerve cell

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-Muscimol

Substance Dose

2 nM

Measurement Parameter

Bmax

Unit

fmol/mg

Quantitative value

70

Deviation

10

Concomitants: Compound RN

759557

Concomitants: Compound name

Clonazepam

Concomitants: Compound role

NSB

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Biological Species/NCBI ID

rat

Organs/Tissues

forebrain

Cells/Cell Lines

nerve cell

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-Muscimol

Substance Dose

2 nM

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Quantitative value

25

Deviation

6.10000

Measurement pX

7.6

Concomitants: Compound RN

1982052; 759557

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

35/94

2018-08-03 20:32:29

47 of 189

48 of 189

Concomitants: Compound name

Honokiol; Clonazepam

Concomitants: Compound role

COM; NSB

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Biological Species/NCBI ID

rat

Organs/Tissues

forebrain

Cells/Cell Lines

nerve cell

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-Muscimol

Substance Dose

2 nM

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Quantitative value

24

Deviation

3

Measurement pX

7.62

Concomitants: Compound RN

1982224; 759557

Concomitants: Compound name

Magnolol; Clonazepam

Concomitants: Compound role

COM; NSB

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

36/94

2018-08-03 20:32:29

49 of 189

50 of 189

Biological Species/NCBI ID

rat

Organs/Tissues

forebrain

Cells/Cell Lines

nerve cell

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-Muscimol

Substance Dose

2 nM

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Quantitative value

21

Deviation

3.30000

Measurement pX

7.68

Concomitants: Compound RN

759557

Concomitants: Compound name

Clonazepam

Concomitants: Compound role

NSB

Target Name

Gamma-aminobutyric acid receptor subunit alpha-6 [rat];+Gamma-aminobutyric acid receptor subunit gamma-2 [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat]

Target Synonyms

gaba(a) receptor subunit alpha-6; gabra-6; gabra6; gamma-aminobutyric acid receptor subunit alpha-6 + gaba(a) receptor subunit gamma-2; gabrg2; gamma-aminobutyric acid receptor subunit gamma-2 + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2

Target Uniprot ID

p30191 + p18508 + p63138

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-6 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit gamma-2 [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit alpha-6 [rat]:Wild + Gamma-aminobutyric acid receptor subunit gamma-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Name

Binding

Cells/Cell Lines

HEK293 cell line

Cell Fraction

Membrane

Substance RN

774694View in Reaxys

Substance Name

[3H]-muscimol

Measurement Parameter

Kd (dissociation constant)

Unit

nM

Quantitative value

5

Deviation

0.500000

Measurement pX

8.3

Target Name

Gamma-aminobutyric acid receptor subunit delta [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat];+Gamma-aminobutyric acid receptor subunit alpha-1 [rat]

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

37/94

2018-08-03 20:32:29

51 of 189

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p18506 + p63138 + p62813

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit delta [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Wild

Target Species (Bioactivity)

rat

Substance Action on Target

Inhibitor

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Inhibitory concentration of the compound against GABA A receptor in rat cerebral cortex using [3H]muscimol (5 nM) as radioligand upon incubation for 10 min at 4 degree C

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Measurement Parameter

IC50

Unit

nM

Qualitative value

=

Quantitative value

16

Measurement pX

7.8

Concomitants: Compound name

[3H]Muscimol

Concomitants: Compound role

RAD

Target Name

Gamma-aminobutyric acid receptor subunit delta;+Gamma-aminobutyric acid receptor subunit beta-2;+Gamma-aminobutyric acid receptor subunit alpha-1

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit delta

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta:Wild + Gamma-aminobutyric acid receptor subunit beta-2:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Binding affinity of the compound towards GABAA receptor using [3H]GABA as radioligand

Substance RN

774694View in Reaxys

Measurement Parameter

Ki (inhibition constant)

Unit

nM

Qualitative value

=

Quantitative value

2.6

Measurement pX

8.59

Concomitants: Compound name

[3H]GABA

Concomitants: Compound role

RAD

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

38/94

2018-08-03 20:32:29

52 of 189

53 of 189

Target Name

Gamma-aminobutyric acid receptor subunit delta;+Gamma-aminobutyric acid receptor subunit beta-2;+Gamma-aminobutyric acid receptor subunit alpha-1

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit delta

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta:Wild + Gamma-aminobutyric acid receptor subunit beta-2:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Percent specific binding of the compound towards GABAA receptor using [3H]GABA as radioligand

Substance RN

774694View in Reaxys

Measurement Parameter

Activity

Unit

%

Qualitative value

=

Quantitative value

90

Concomitants: Compound name

[3H]GABA

Concomitants: Compound role

RAD

Bioassay Category

In Vivo (Animal models)

Bioassay Details

In vivo percent slow oscillation (OS) of firing rate of DA neurons upon 2 mg/kg of intravenous adminitration with compound; n=16

Biological Species/NCBI ID

rat

Cells/Cell Lines

nerve cell

Substance RN

774694View in Reaxys

Substance Route of Adm.

intravenous administration

Qualitative Results

Reduced from 4.45 +/- 0.37 %

Measurement Parameter

percentage

Unit

%

Measurement Object

firing rate

Qualitative value

=

Quantitative value

2.18

Deviation

0.4

Xu, Dan; Karain, Bradley; Brantley, Eileen; Shi, Wei-Xing; Journal of Pharmacology and Experimental Therapeutics; vol.

337; nb. 2; (2011); p. 533 - 539, View in Reaxys 54 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

In vivo effect on cell Firing rate of DA neurons in rat upon 2 mg/kg of intravenous adminitration with compound

Biological Species/NCBI ID

rat

Cells/Cell Lines

nerve cell

Substance RN

774694View in Reaxys

Substance Route of Adm.

intravenous administration

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

39/94

2018-08-03 20:32:29

Qualitative Results

Increased from 4.83 +/- 0.40 spikes/s

Measurement Parameter

qualitative

Measurement Object

firing rate

Xu, Dan; Karain, Bradley; Brantley, Eileen; Shi, Wei-Xing; Journal of Pharmacology and Experimental Therapeutics; vol.

337; nb. 2; (2011); p. 533 - 539, View in Reaxys 55 of 189

Target Name

Gamma-aminobutyric acid receptor subunit delta [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat];+Gamma-aminobutyric acid receptor subunit alpha-1 [rat]

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p18506 + p63138 + p62813

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit delta [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Wild

Target Species (Bioactivity)

rat

Substance Action on Target

Inhibitor

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

In vitro inhibitory concentration of compound against GABA receptor binding to membrane isolated from rat brain

Biological Species/NCBI ID

rat

Cells/Cell Lines

brain cell

Substance RN

774694View in Reaxys

Measurement Parameter

IC50

Unit

ÂľM

Qualitative value

=

Quantitative value

0.024

Deviation

0.003

Measurement pX

7.62

Patent; H. Lundbeck and Co. A/S; US4278676; (1981); (A) English, View in Reaxys 56 of 189

Bioassay Category

Toxicity/Safety Pharmacology

Bioassay Details

Lethal dose of the compound was determined in mice upon intravenous administration of compound

Biological Species/NCBI ID

mouse

Substance RN

774694View in Reaxys

Substance Route of Adm.

intravenous administration

Measurement Parameter

LD50

Unit

mg/kg

Qualitative value

=

Quantitative value

7

Patent; H. Lundbeck and Co. A/S; US4278676; (1981); (A) English, View in Reaxys 57 of 189

Bioassay Category

Toxicity/Safety Pharmacology

Bioassay Details

Lethal dose of the compound was determined in mice upon oral administration of compound

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

40/94

2018-08-03 20:32:29

Biological Species/NCBI ID

mouse

Substance RN

774694View in Reaxys

Substance Route of Adm.

oral administration

Measurement Parameter

LD50

Unit

mg/kg

Measurement Object

Lethal dose of the compound in mouse

Qualitative value

=

Quantitative value

22

Patent; H. Lundbeck and Co. A/S; US4278676; (1981); (A) English, View in Reaxys 58 of 189

Bioassay Category

Toxicity/Safety Pharmacology

Bioassay Details

Lethal dose of the compound was determined in mice upon intraperitonial administration of compound

Biological Species/NCBI ID

mouse

Substance RN

774694View in Reaxys

Substance Route of Adm.

intraperitoneal administration

Measurement Parameter

LD50

Unit

mg/kg

Qualitative value

=

Quantitative value

12

Patent; H. Lundbeck and Co. A/S; US4278676; (1981); (A) English, View in Reaxys 59 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

In vivo percent slow oscillation (OS) of firing rate of DA neurons upon 2 mg/kg of intravenous adminitration with compound

Biological Species/NCBI ID

rat

Cells/Cell Lines

nerve cell

Substance RN

774694View in Reaxys

Substance Route of Adm.

intravenous administration

Qualitative Results

Decreased from 0.23 +/- 0.03 %

Measurement Parameter

percentage

Unit

%

Measurement Object

firing rate

Qualitative value

=

Quantitative value

0.07

Deviation

0.01

Xu, Dan; Karain, Bradley; Brantley, Eileen; Shi, Wei-Xing; Journal of Pharmacology and Experimental Therapeutics; vol.

337; nb. 2; (2011); p. 533 - 539, View in Reaxys 60 of 189

Target Name

Gamma-aminobutyric acid receptor subunit delta;+Gamma-aminobutyric acid receptor subunit beta-2;+Gamma-aminobutyric acid receptor subunit alpha-1

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit delta

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

41/94

2018-08-03 20:32:29

Target Mutant/Chimera Details

61 of 189

62 of 189

Gamma-aminobutyric acid receptor subunit delta:Wild + Gamma-aminobutyric acid receptor subunit beta-2:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Percent inhibition of GABA A (agonist site) at a compound concentration of 0.00001 mol/L was determined

Substance RN

774694View in Reaxys

Measurement Parameter

inhibition rate

Unit

%

Qualitative value

=

Quantitative value

100

Target Name

Solute carrier family 22 member 1 [rat]

Target Synonyms

oct1; organic cation transporter 1; roct1; slc22a1; solute carrier family 22 member 1

Target Uniprot ID

q63089

Target, Subunit, Species

Solute carrier family 22 member 1 [rat]

Target Mutant/Chimera Details

Solute carrier family 22 member 1 [rat]:Wild

Target Species (Bioactivity)

rat

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Percent inhibition of 1 uM [3H]MPP+ (1-methyl-4-phenylpyridinium) uptake mediated by Organic cation transporter 1 into cryopreserved rat hepatocytes upon incubation at 37 degree C for 5 min with compound at a concentration of 20 uM

Biological Species/NCBI ID

rat

Cells/Cell Lines

liver cell

Substance RN

774694View in Reaxys

Measurement Parameter

inhibition rate

Unit

%

Measurement Object

[3H]MPP+ uptake

Qualitative value

=

Quantitative value

35

Deviation

15

Concomitants: Compound name

[3H]MPP+

Concomitants: Compound role

SUB

Target Name

Solute Carrier Organic Anion Transporter Family, Member 1B1 - Solute Carrier Organic Anion Transporter Family, Member 1B3 [rat]

Target Synonyms

solute carrier organic anion transporter family, member 1b1 - solute carrier organic anion transporter family, member 1b3

Target, Subunit, Species

Solute Carrier Organic Anion Transporter Family, Member 1B1 - Solute Carrier Organic Anion Transporter Family, Member 1B3 [rat]

Target Mutant/Chimera Details

Solute Carrier Organic Anion Transporter Family, Member 1B1 - Solute Carrier Organic Anion Transporter Family, Member 1B3 [rat]:Wild

Target Species (Bioactivity)

rat

Bioassay Category

In Vitro (Efficacy)

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

42/94

2018-08-03 20:32:29

63 of 189

64 of 189

Bioassay Details

Percent inhibition of 1 uM [3H]Estradiol-17beta-glucuronide (E217G) uptake mediated by OATP1B1/3 into cryopreserved rat hepatocytes upon incubation at 37 degree C for 5 min with compound at a concentration of 20 uM

Biological Species/NCBI ID

rat

Cells/Cell Lines

liver cell

Substance RN

774694View in Reaxys

Measurement Parameter

inhibition rate

Unit

%

Measurement Object

[3h]Estradiol-17beta-Glucuronide Uptake

Qualitative value

=

Quantitative value

47

Deviation

26

Concomitants: Compound name

[3H]ESTRADIOL-17BETA-GLUCURONIDE

Concomitants: Compound role

SUB

Target Name

Gamma-aminobutyric acid receptor subunit delta [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

o14764 + p47870 + p14867

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit delta [human]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild

Target Species (Bioactivity)

human

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Inhibitory concentration of the compound (agonist radioligand) against Human GABAA RECEPTOR ALPHA1BETA2GAMMA2 was determined

Biological Species/NCBI ID

human

Substance RN

774694View in Reaxys

Measurement Parameter

IC50

Unit

M

Qualitative value

=

Quantitative value

1.4E-08

Measurement pX

7.85

Target Name

Gamma-aminobutyric acid receptor subunit delta [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

o14764 + p47870 + p14867

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit delta [human]

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

43/94

2018-08-03 20:32:29

65 of 189

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild

Target Species (Bioactivity)

human

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Inhibition constant of the compound (agonist radioligand) against Human GABAA RECEPTOR ALPHA1BETA2GAMMA2 was determined

Biological Species/NCBI ID

human

Substance RN

774694View in Reaxys

Measurement Parameter

Ki (inhibition constant)

Unit

M

Qualitative value

=

Quantitative value

9.6E-09

Measurement pX

8.02

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Effect of the compound on percent rate of nonresponse (NR) task in rat after intraperitoneal dose of 3 mg/kg; n=6, Vehicle= 0.0+/-0.0

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Substance Route of Adm.

intraperitoneal administration

Measurement Parameter

Activity

Unit

%

Measurement Object

Effect

Qualitative value

=

Quantitative value

93.9

Deviation

3

Mishima, Kenichi; Fujii, Megumi; Aoo, Naoya; Yoshikawa, Tetsuya; Fukue, Yoshihiko; Honda, Yoko; Egashira, Nobuaki; Iwasaki, Katsunori; Shoyama, Yukihiro; Fujiwara, Michihiro; Biological and pharmaceutical bulletin; vol. 25; nb. 12; (2002); p. 1570 - 1576, View in Reaxys 66 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Effect of the compound on percent rate of nonresponse (NR) task in rat after intraperitoneal dose of 1 mg/kg; n=4, Vehicle= 0.0+/-0.0

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Substance Route of Adm.

intraperitoneal administration

Measurement Parameter

Activity

Unit

%

Measurement Object

Effect

Qualitative value

=

Quantitative value

0.8

Deviation

0.8

Mishima, Kenichi; Fujii, Megumi; Aoo, Naoya; Yoshikawa, Tetsuya; Fukue, Yoshihiko; Honda, Yoko; Egashira, Nobuaki; Iwasaki, Katsunori; Shoyama, Yukihiro; Fujiwara, Michihiro; Biological and pharmaceutical bulletin; vol. 25; nb. 12; (2002); p. 1570 - 1576, View in Reaxys

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

44/94

2018-08-03 20:32:29

67 of 189

Bioassay Category

In Vivo (Animal models)

Bioassay Details

Effect of compound (0.2 mg/kg) on number of mice falling asleep when treated by sub-hypnotic dosage of pentobarbital (28 mg/kg) administered intraperitoneally; n=15

Biological Species/NCBI ID

mouse

Substance RN

774694View in Reaxys

Substance Route of Adm.

intravenous administration

Qualitative Results

No. of mice not calculated

Measurement Parameter

qualitative

Measurement Object

No. of mice

Concomitants: Compound name

pentobarbital (28 mg/kg)

Concomitants: Compound role

COM

Ma, Yuan; Han, Huishan; Eun, Jae Soon; Kim, Hyung-Chun; Hong, Jin-Tae; Oh, Ki-Wan; Biological and Pharmaceutical Bulletin; vol. 30; nb. 9; (2007); p. 1748 - 1753, View in Reaxys 68 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Effect of the compound on percent rate of choice reactiontime (CRT) task in rat after intraperitoneal dose of 1 mg/kg; n=4, Vehicle= 1.3+/-0.2 s

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Substance Route of Adm.

intraperitoneal administration

Measurement Parameter

Activity

Unit

second

Qualitative value

=

Quantitative value

1.4

Deviation

0.1

Mishima, Kenichi; Fujii, Megumi; Aoo, Naoya; Yoshikawa, Tetsuya; Fukue, Yoshihiko; Honda, Yoko; Egashira, Nobuaki; Iwasaki, Katsunori; Shoyama, Yukihiro; Fujiwara, Michihiro; Biological and pharmaceutical bulletin; vol. 25; nb. 12; (2002); p. 1570 - 1576, View in Reaxys 69 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Effect of the compound on percent rate of error response (ER) task in rat after intraperitoneal dose of 1 mg/kg; n=4, Vehicle= 0.2+/-0.2

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Substance Route of Adm.

intraperitoneal administration

Measurement Parameter

Activity

Unit

%

Measurement Object

Effect

Qualitative value

=

Quantitative value

0

Deviation

0

Mishima, Kenichi; Fujii, Megumi; Aoo, Naoya; Yoshikawa, Tetsuya; Fukue, Yoshihiko; Honda, Yoko; Egashira, Nobuaki; Iwasaki, Katsunori; Shoyama, Yukihiro; Fujiwara, Michihiro; Biological and pharmaceutical bulletin; vol. 25; nb. 12; (2002); p. 1570 - 1576, View in Reaxys 70 of 189

Bioassay Category

In Vitro (Efficacy)

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

45/94

2018-08-03 20:32:29

Bioassay Details

Number of mice falling asleep after administration of 10 mg/kg of compound was determined; n=15

Biological Species/NCBI ID

mouse

Substance RN

774694View in Reaxys

Qualitative Results

No. of mice not calculated

Measurement Parameter

qualitative

Measurement Object

No. of mice

Ma, Yuan; Han, Huishan; Eun, Jae Soon; Kim, Hyung-Chun; Hong, Jin-Tae; Oh, Ki-Wan; Biological and Pharmaceutical Bulletin; vol. 30; nb. 9; (2007); p. 1748 - 1753, View in Reaxys 71 of 189

Bioassay Category

In Vivo (Animal models)

Bioassay Details

Effect of the compound on percent rate of choice reactiontime (CRT) task in rat after intraperitoneal dose of 3 mg/kg; n=6, Vehicle= 1.3+/-0.2 s

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Substance Route of Adm.

intraperitoneal administration

Measurement Parameter

Activity

Unit

second

Qualitative value

=

Quantitative value

3.3

Deviation

1.2

Mishima, Kenichi; Fujii, Megumi; Aoo, Naoya; Yoshikawa, Tetsuya; Fukue, Yoshihiko; Honda, Yoko; Egashira, Nobuaki; Iwasaki, Katsunori; Shoyama, Yukihiro; Fujiwara, Michihiro; Biological and pharmaceutical bulletin; vol. 25; nb. 12; (2002); p. 1570 - 1576, View in Reaxys 72 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Activity of the compound in rat after intraperitoneal dose of 3 mg/kg after 30 trials; n=6, Vehicle= 598.0+/-22.5

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Substance Route of Adm.

intraperitoneal administration

Measurement Parameter

Activity

Quantitative value

175.5

Mishima, Kenichi; Fujii, Megumi; Aoo, Naoya; Yoshikawa, Tetsuya; Fukue, Yoshihiko; Honda, Yoko; Egashira, Nobuaki; Iwasaki, Katsunori; Shoyama, Yukihiro; Fujiwara, Michihiro; Biological and pharmaceutical bulletin; vol. 25; nb. 12; (2002); p. 1570 - 1576, View in Reaxys 73 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Effect of the compound on total number of lever pressings in rat upon intraperitoneal administration at 0.3 mg/Kg dosage; n=6, Vehicle= 6.7+/-1.6

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Substance Route of Adm.

intraperitoneal administration

Measurement Parameter

Activity

Quantitative value

7.2

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

46/94

2018-08-03 20:32:29

Mishima, Kenichi; Fujii, Megumi; Aoo, Naoya; Yoshikawa, Tetsuya; Fukue, Yoshihiko; Honda, Yoko; Egashira, Nobuaki; Iwasaki, Katsunori; Shoyama, Yukihiro; Fujiwara, Michihiro; Biological and pharmaceutical bulletin; vol. 25; nb. 12; (2002); p. 1570 - 1576, View in Reaxys 74 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Effect of compound (0.2 mg/kg) on sleeping time of mice falling asleep when treated by sub-hypnotic dosage of pentobarbital (28 mg/kg) administered intraperitoneally; n=15

Biological Species/NCBI ID

mouse

Substance RN

774694View in Reaxys

Substance Route of Adm.

intravenous administration

Measurement Parameter

sleep time

Unit

minute

Measurement Object

Sleeping time

Qualitative value

=

Quantitative value

55.4

Deviation

11.4

Concomitants: Compound name

pentobarbital (28 mg/kg)

Concomitants: Compound role

COM

Ma, Yuan; Han, Huishan; Eun, Jae Soon; Kim, Hyung-Chun; Hong, Jin-Tae; Oh, Ki-Wan; Biological and Pharmaceutical Bulletin; vol. 30; nb. 9; (2007); p. 1748 - 1753, View in Reaxys 75 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Effect of the compound on percent rate of error response (ER) task in rat after intraperitoneal dose of 0.3 mg/kg; n=6, Vehicle= 0.2+/-0.2

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Substance Route of Adm.

intraperitoneal administration

Measurement Parameter

Activity

Unit

%

Measurement Object

Effect

Qualitative value

=

Quantitative value

0.3

Deviation

0.2

Mishima, Kenichi; Fujii, Megumi; Aoo, Naoya; Yoshikawa, Tetsuya; Fukue, Yoshihiko; Honda, Yoko; Egashira, Nobuaki; Iwasaki, Katsunori; Shoyama, Yukihiro; Fujiwara, Michihiro; Biological and pharmaceutical bulletin; vol. 25; nb. 12; (2002); p. 1570 - 1576, View in Reaxys 76 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Effect of the compound on percent rate of correct response (CR) task in rat after intraperitoneal dose of 1 mg/kg; n=4, Vehicle= 99.5+/-0.6

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Substance Route of Adm.

intraperitoneal administration

Measurement Parameter

Activity

Unit

%

Measurement Object

Effect

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

47/94

2018-08-03 20:32:29

Qualitative value

=

Quantitative value

99.2

Deviation

0.8

Mishima, Kenichi; Fujii, Megumi; Aoo, Naoya; Yoshikawa, Tetsuya; Fukue, Yoshihiko; Honda, Yoko; Egashira, Nobuaki; Iwasaki, Katsunori; Shoyama, Yukihiro; Fujiwara, Michihiro; Biological and pharmaceutical bulletin; vol. 25; nb. 12; (2002); p. 1570 - 1576, View in Reaxys 77 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Activity of the compound in rat after intraperitoneal dose of 1 mg/kg after 30 trials; n=4, Vehicle= 598.0+/-22.5

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Substance Route of Adm.

intraperitoneal administration

Measurement Parameter

Activity

Quantitative value

644.5

Mishima, Kenichi; Fujii, Megumi; Aoo, Naoya; Yoshikawa, Tetsuya; Fukue, Yoshihiko; Honda, Yoko; Egashira, Nobuaki; Iwasaki, Katsunori; Shoyama, Yukihiro; Fujiwara, Michihiro; Biological and pharmaceutical bulletin; vol. 25; nb. 12; (2002); p. 1570 - 1576, View in Reaxys 78 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Activity of the compound in rat after intraperitoneal dose of 0.3 mg/kg after 30 trials; n=6, Vehicle= 598.0+/-22.5

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Substance Route of Adm.

intraperitoneal administration

Measurement Parameter

Activity

Quantitative value

604

Mishima, Kenichi; Fujii, Megumi; Aoo, Naoya; Yoshikawa, Tetsuya; Fukue, Yoshihiko; Honda, Yoko; Egashira, Nobuaki; Iwasaki, Katsunori; Shoyama, Yukihiro; Fujiwara, Michihiro; Biological and pharmaceutical bulletin; vol. 25; nb. 12; (2002); p. 1570 - 1576, View in Reaxys 79 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Effect of the compound on total number of lever pressings in rat upon intraperitoneal administration at 1 mg/Kg dosage; n=4, Vehicle= 6.7+/-1.6

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Substance Route of Adm.

intraperitoneal administration

Measurement Parameter

Activity

Quantitative value

6.5

Mishima, Kenichi; Fujii, Megumi; Aoo, Naoya; Yoshikawa, Tetsuya; Fukue, Yoshihiko; Honda, Yoko; Egashira, Nobuaki; Iwasaki, Katsunori; Shoyama, Yukihiro; Fujiwara, Michihiro; Biological and pharmaceutical bulletin; vol. 25; nb. 12; (2002); p. 1570 - 1576, View in Reaxys 80 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Effect of the compound on percent rate of correct response (CR) task in rat after intraperitoneal dose of 0.3 mg/kg; n=6, Vehicle= 99.5+/-0.6

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Substance Route of Adm.

intraperitoneal administration

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

48/94

2018-08-03 20:32:29

Measurement Parameter

Activity

Unit

%

Measurement Object

Effect

Qualitative value

=

Quantitative value

96.1

Deviation

1.6

Mishima, Kenichi; Fujii, Megumi; Aoo, Naoya; Yoshikawa, Tetsuya; Fukue, Yoshihiko; Honda, Yoko; Egashira, Nobuaki; Iwasaki, Katsunori; Shoyama, Yukihiro; Fujiwara, Michihiro; Biological and pharmaceutical bulletin; vol. 25; nb. 12; (2002); p. 1570 - 1576, View in Reaxys 81 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Sleeping time of the mice after administration of 10 mg/kg of compound was determined

Biological Species/NCBI ID

mouse

Substance RN

774694View in Reaxys

Measurement Parameter

sleep time

Unit

minute

Measurement Object

Sleeping time

Qualitative value

=

Quantitative value

0

Ma, Yuan; Han, Huishan; Eun, Jae Soon; Kim, Hyung-Chun; Hong, Jin-Tae; Oh, Ki-Wan; Biological and Pharmaceutical Bulletin; vol. 30; nb. 9; (2007); p. 1748 - 1753, View in Reaxys 82 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Effect of the compound on percent rate of correct response (CR) task in rat after intraperitoneal dose of 3 mg/kg; n=6, Vehicle= 99.5+/-0.6

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Substance Route of Adm.

intraperitoneal administration

Measurement Parameter

Activity

Unit

%

Measurement Object

Effect

Qualitative value

=

Quantitative value

3.3

Deviation

2.1

Mishima, Kenichi; Fujii, Megumi; Aoo, Naoya; Yoshikawa, Tetsuya; Fukue, Yoshihiko; Honda, Yoko; Egashira, Nobuaki; Iwasaki, Katsunori; Shoyama, Yukihiro; Fujiwara, Michihiro; Biological and pharmaceutical bulletin; vol. 25; nb. 12; (2002); p. 1570 - 1576, View in Reaxys 83 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Effect of the compound on percent rate of error response (ER) task in rat after intraperitoneal dose of 3 mg/kg; n=6, Vehicle= 0.2+/-0.2

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Substance Route of Adm.

intraperitoneal administration

Measurement Parameter

Activity

Unit

%

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

49/94

2018-08-03 20:32:29

Measurement Object

Effect

Qualitative value

=

Quantitative value

2.2

Deviation

1.4

Mishima, Kenichi; Fujii, Megumi; Aoo, Naoya; Yoshikawa, Tetsuya; Fukue, Yoshihiko; Honda, Yoko; Egashira, Nobuaki; Iwasaki, Katsunori; Shoyama, Yukihiro; Fujiwara, Michihiro; Biological and pharmaceutical bulletin; vol. 25; nb. 12; (2002); p. 1570 - 1576, View in Reaxys 84 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Effect of the compound on total number of lever pressings in rat upon intraperitoneal administration at 1 mg/Kg dosage; n=6, Vehicle= 6.7+/-1.6

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Substance Route of Adm.

intraperitoneal administration

Measurement Parameter

Activity

Quantitative value

7.5

Mishima, Kenichi; Fujii, Megumi; Aoo, Naoya; Yoshikawa, Tetsuya; Fukue, Yoshihiko; Honda, Yoko; Egashira, Nobuaki; Iwasaki, Katsunori; Shoyama, Yukihiro; Fujiwara, Michihiro; Biological and pharmaceutical bulletin; vol. 25; nb. 12; (2002); p. 1570 - 1576, View in Reaxys 85 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Effect of the compound on percent rate of choice reactiontime (CRT) task in rat after intraperitoneal dose of 0.3 mg/kg; n=6, Vehicle= 1.3+/-0.2 s

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Substance Route of Adm.

intraperitoneal administration

Measurement Parameter

Activity

Unit

second

Qualitative value

=

Quantitative value

1.3

Deviation

0.2

Mishima, Kenichi; Fujii, Megumi; Aoo, Naoya; Yoshikawa, Tetsuya; Fukue, Yoshihiko; Honda, Yoko; Egashira, Nobuaki; Iwasaki, Katsunori; Shoyama, Yukihiro; Fujiwara, Michihiro; Biological and pharmaceutical bulletin; vol. 25; nb. 12; (2002); p. 1570 - 1576, View in Reaxys 86 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Effect of the compound on percent rate of nonresponse (NR) task in rat after intraperitoneal dose of 0.3 mg/kg; n=6, Vehicle= 0.0+/-0.0

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Substance Route of Adm.

intraperitoneal administration

Measurement Parameter

Activity

Unit

%

Measurement Object

Effect

Qualitative value

=

Quantitative value

0.8

Deviation

0.5

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

50/94

2018-08-03 20:32:29

Mishima, Kenichi; Fujii, Megumi; Aoo, Naoya; Yoshikawa, Tetsuya; Fukue, Yoshihiko; Honda, Yoko; Egashira, Nobuaki; Iwasaki, Katsunori; Shoyama, Yukihiro; Fujiwara, Michihiro; Biological and pharmaceutical bulletin; vol. 25; nb. 12; (2002); p. 1570 - 1576, View in Reaxys 87 of 189

88 of 189

Target Name

Gamma-aminobutyric acid receptor subunit delta [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat];+Gamma-aminobutyric acid receptor subunit alpha-1 [rat]

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p18506 + p63138 + p62813

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit delta [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Wild

Target Species (Bioactivity)

rat

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Percent displacement of [3H]GABA from GABA receptor in rat brain membranes at a compound concentration of 0.01 uM

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Substance Dose

0.01 Âľm

Measurement Parameter

percentage

Unit

%

Measurement Object

Displacement

Qualitative value

=

Quantitative value

86

Deviation

3

Concomitants: Compound name

[3H]GABA

Concomitants: Compound role

RAD

Target Name

Gamma-aminobutyric acid receptor subunit delta [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat];+Gamma-aminobutyric acid receptor subunit alpha-1 [rat]

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p18506 + p63138 + p62813

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit delta [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Wild

Target Species (Bioactivity)

rat

Substance Action on Target

Inhibitor

Bioassay Category

In Vitro (Efficacy)

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

51/94

2018-08-03 20:32:29

89 of 189

Bioassay Details

Inhibitory concentration of the compound against binding of [3H]GABA (8.0 nM) to rat brain tissue sodium-independent Gamma-aminobutyric-acid receptor upon incubation in 0.05 M Tris-citrate buffer ,pH 7.1 for 5 minutes at 4 degree C

Biological Species/NCBI ID

rat

Cells/Cell Lines

brain cell

Substance RN

774694View in Reaxys

Measurement Parameter

IC50

Unit

M

Qualitative value

=

Quantitative value

3.2E-09

Deviation

0.0000000001

Measurement pX

8.49

Concomitants: Compound name

[3H]GABA

Concomitants: Compound role

RAD

Target Name

Gamma-aminobutyric acid receptor subunit delta [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat];+Gamma-aminobutyric acid receptor subunit alpha-1 [rat]

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p18506 + p63138 + p62813

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit delta [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Wild

Target Species (Bioactivity)

rat

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Binding affinity of compound towards GABA receptor in rat whole brain using 4e-9 M [3H]gamma-amino butyric acid as radioligand

Biological Species/NCBI ID

rat

Organs/Tissues

brain

Substance RN

774694View in Reaxys

Measurement Parameter

Ki (inhibition constant)

Unit

ÂľM

Qualitative value

=

Quantitative value

0.004

Deviation

0.001

Measurement pX

8.4

Concomitants: Compound name

[3H]GABA

Concomitants: Compound role

RAD

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

52/94

2018-08-03 20:32:29

Kaplan; Raizon; Desarmenien; Feltz; Headley; Worms; Lloyd; Bartholini; Journal of Medicinal Chemistry; vol. 23; nb. 6; (1980); p. 702 - 704, View in Reaxys 90 of 189

Target Name

Gamma-aminobutyric acid receptor subunit delta [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

o14764 + p47870 + p14867

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit delta [human]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild

Target Species (Bioactivity)

human

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Binding affinity was evaluated by displacement of [3H]gamma-amino butyric acid form GABA receptor in human cerebellar cortex preparation

Biological Species/NCBI ID

human

Cells/Cell Lines

Brain cerebrocortical cell

Substance RN

774694View in Reaxys

Measurement Parameter

Ki (inhibition constant)

Unit

ÂľM

Qualitative value

=

Quantitative value

0.01

Deviation

0.002

Measurement pX

8

Concomitants: Compound name

[3H]GABA

Concomitants: Compound role

RAD

Kaplan; Raizon; Desarmenien; Feltz; Headley; Worms; Lloyd; Bartholini; Journal of Medicinal Chemistry; vol. 23; nb. 6; (1980); p. 702 - 704, View in Reaxys 91 of 189

Target Name

Gamma-aminobutyric acid receptor subunit delta [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat];+Gamma-aminobutyric acid receptor subunit alpha-1 [rat]

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p18506 + p63138 + p62813

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit delta [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Wild

Target Species (Bioactivity)

rat

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

53/94

2018-08-03 20:32:29

92 of 189

Bioassay Details

Relative binding affinity of the compound against binding of [3H]GABA (8.0 nM) to rat brain tissue sodium-independent Gamma-aminobutyric-acid receptor upon incubation in 0.05 M Tris-citrate buffer ,pH 7.1 for 5 minutes at 4 degree C

Biological Species/NCBI ID

rat

Cells/Cell Lines

brain cell

Substance RN

774694View in Reaxys

Measurement Parameter

Activity

Quantitative value

6.3

Concomitants: Compound name

[3H]GABA

Concomitants: Compound role

RAD

Bioassay Category

Toxicity/Safety Pharmacology

Bioassay Details

Lethal dose which causes death in 50 of the mice within 48 hours of i.p. administration

Biological Species/NCBI ID

mouse

Substance RN

774694View in Reaxys

Substance Route of Adm.

intraperitoneal administration

Measurement Parameter

LD50

Unit

mg/kg

Qualitative value

=

Quantitative value

17

Deviation

17.3

Kaplan; Raizon; Desarmenien; Feltz; Headley; Worms; Lloyd; Bartholini; Journal of Medicinal Chemistry; vol. 23; nb. 6; (1980); p. 702 - 704, View in Reaxys 93 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Effective dose which decreases the 50 of the convulsions induced by bicuculline in mice

Biological Species/NCBI ID

mouse

Substance RN

774694View in Reaxys

Measurement Parameter

ED50

Unit

mg/kg

Qualitative value

=

Quantitative value

0.25

Deviation

0.1

Kaplan; Raizon; Desarmenien; Feltz; Headley; Worms; Lloyd; Bartholini; Journal of Medicinal Chemistry; vol. 23; nb. 6; (1980); p. 702 - 704, View in Reaxys 94 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Effective dose which decreases the 50 of the lethality induced by bicuculline in mice

Biological Species/NCBI ID

mouse

Substance RN

774694View in Reaxys

Measurement Parameter

ED50

Unit

mg/kg

Measurement Object

Lethality induced by bicuculline

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

54/94

2018-08-03 20:32:29

Qualitative value

>

Quantitative value

2

Kaplan; Raizon; Desarmenien; Feltz; Headley; Worms; Lloyd; Bartholini; Journal of Medicinal Chemistry; vol. 23; nb. 6; (1980); p. 702 - 704, View in Reaxys 95 of 189

96 of 189

Target Name

Gamma-aminobutyric acid receptor subunit delta [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

o14764 + p47870 + p14867

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit delta [human]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild

Target Species (Bioactivity)

human

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Inhibitory concentration of the compound against human GABAA RECEPTOR ALPHA1BETA2GAMMA2 expressed in CHO cells using 15 nM [3H]MUSCIMOL as radioligand upon incubation for 120 min at RT

Biological Species/NCBI ID

human

Cells/Cell Lines

CHO cell line

Substance RN

774694View in Reaxys

Measurement Parameter

IC50

Unit

M

Qualitative value

=

Quantitative value

7.2E-08

Measurement pX

7.14

Concomitants: Compound name

[3H]MUSCIMOL

Concomitants: Compound role

RAD

Target Name

Gamma-aminobutyric acid receptor subunit delta [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

o14764 + p47870 + p14867

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit delta [human]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild

Target Species (Bioactivity)

human

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Binding affinity of the compound towards human GABAA RECEPTOR ALPHA1BETA2GAMMA2 expressed in CHO cells using 15 nM [3H]MUSCIMOL as radioligand upon incubation for 120 min at RT

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

55/94

2018-08-03 20:32:29

Biological Species/NCBI ID

97 of 189

human

Cells/Cell Lines

CHO cell line

Substance RN

774694View in Reaxys

Measurement Parameter

Ki (inhibition constant)

Unit

M

Qualitative value

=

Quantitative value

4.8E-08

Measurement pX

7.32

Concomitants: Compound name

[3H]MUSCIMOL

Concomitants: Compound role

RAD

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

BMC-sensitive inhibitory effect of the compound on cat spinal neurons relative to that of GABA was expressed in vivo as relative potency; ---- = Greater activity

Biological Species/NCBI ID

cat

Cells/Cell Lines

spinal cord nerve cell

Substance RN

774694View in Reaxys

Qualitative Results

----

Measurement Object

Inhibitory effect

Krogsgaard-Larsen; Nielsen; Falch; Curtis; Journal of Medicinal Chemistry; vol. 28; nb. 11; (1985); p. 1612 - 1617, View in Reaxys 98 of 189

Target Name

Gamma-aminobutyric acid receptor subunit delta [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat];+Gamma-aminobutyric acid receptor subunit alpha-1 [rat]

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p18506 + p63138 + p62813

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit delta [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Wild

Target Species (Bioactivity)

rat

Substance Action on Target

Inhibitor

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

In vitro inhibitory activity against gamma-aminobutyric-acid receptor in rat brain synaptic membranes using [3H]P4S (0.005 uM) as radioligand upon incubation for 15 min at 4 degree C in 0.05 M Tris-citrate buffer (pH 7.1)

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Measurement Parameter

IC50

Unit

ÂľM

Qualitative value

=

Quantitative value

0.006

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

56/94

2018-08-03 20:32:29

Measurement pX

8.22

Concomitants: Compound name

[3H]P4S

Concomitants: Compound role

RAD

Krogsgaard-Larsen; Nielsen; Falch; Curtis; Journal of Medicinal Chemistry; vol. 28; nb. 11; (1985); p. 1612 - 1617, View in Reaxys 99 of 189

100 of 189

Target Name

Gamma-aminobutyric acid receptor subunit delta [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat];+Gamma-aminobutyric acid receptor subunit alpha-1 [rat]

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p18506 + p63138 + p62813

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit delta [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Wild

Target Species (Bioactivity)

rat

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Ability to displace 4 nM [3H] muscimol binding from GABA receptor in rat cerebellum membrane

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Measurement Parameter

IC50

Unit

ÂľM

Qualitative value

=

Quantitative value

0.004

Measurement pX

8.4

Concomitants: Compound name

[3H]Muscimol

Concomitants: Compound role

RAD

Target Name

Glutamine synthetase [rat]

Target Synonyms

glns; glul; glutamate decarboxylase; glutamate-ammonia ligase; glutamine synthetase; gs

Target Uniprot ID

p09606

Target, Subunit, Species

Glutamine synthetase [rat]

Target Mutant/Chimera Details

Glutamine synthetase [rat]:Wild

Target Species (Bioactivity)

rat

Substance Action on Target

Inhibitor

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Percent inhibition of glutamic acid decarboxylase of rat diencephalon membranes upon incubation with 50 mM phosphate buffer, pH 6.5 for 30 min at 37 degree C

Biological Species/NCBI ID

rat

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

57/94

2018-08-03 20:32:29

101 of 189

Substance RN

774694View in Reaxys

Qualitative Results

ND

Measurement Parameter

Activity

Measurement Object

Inhibition

Qualitative value

ND

Target Name

GAT [rat]

Target Synonyms

gat

Target, Subunit, Species

GAT [rat]

Target Mutant/Chimera Details

GAT [rat]:Wild

Target Species (Bioactivity)

rat

Substance Action on Target

Inhibitor

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

In vitro gamma-aminobutyric acid transporter activity was determined by the inhibition of GABA (0.05 uM) uptake in rat brain synaptic membranes

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Measurement Parameter

IC50

Unit

ÂľM

Qualitative value

=

Quantitative value

320

Measurement pX

3.49

Krogsgaard-Larsen; Nielsen; Falch; Curtis; Journal of Medicinal Chemistry; vol. 28; nb. 11; (1985); p. 1612 - 1617, View in Reaxys 102 of 189

Target Name

Gamma-aminobutyric acid receptor subunit delta [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat];+Gamma-aminobutyric acid receptor subunit alpha-1 [rat]

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p18506 + p63138 + p62813

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit delta [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Wild

Target Species (Bioactivity)

rat

Substance Action on Target

Inhibitor

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

In vitro inhibitory activity against gamma-aminobutyric-acid receptor in rat brain synaptic membranes using [3H]THIP (0.005 uM) as radioligand upon incubation for 15 min at 4 degree C in 0.05 M Tris-citrate buffer (pH 7.1)

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Measurement Parameter

IC50

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

58/94

2018-08-03 20:32:29

Unit

ÂľM

Qualitative value

=

Quantitative value

0.005

Measurement pX

8.3

Concomitants: Compound name

[3H]THIP

Concomitants: Compound role

RAD

Krogsgaard-Larsen; Nielsen; Falch; Curtis; Journal of Medicinal Chemistry; vol. 28; nb. 11; (1985); p. 1612 - 1617, View in Reaxys 103 of 189

104 of 189

Target Name

Peripheral-type benzodiazepine receptor-associated protein 1 [rat]

Target Synonyms

bzrap1; peripheral-type benzodiazepine receptor-associated protein 1; prax-1; rbp1; rim-bp1; rimsbinding protein 1; tspo-associated protein 1; tspoap1

Target Uniprot ID

q9jir0

Target, Subunit, Species

Peripheral-type benzodiazepine receptor-associated protein 1 [rat]

Target Mutant/Chimera Details

Peripheral-type benzodiazepine receptor-associated protein 1 [rat]:Wild

Target Species (Bioactivity)

rat

Substance Action on Target

Inhibitor

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Percent inhibition of 1.5 nM [3H] diazepam binding to benzodiazepine receptor in rat frontal cortex membranes; ND = no data

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Qualitative Results

ND

Measurement Parameter

Activity

Measurement Object

Inhibition

Qualitative value

ND

Concomitants: Compound name

[3H]Diazepam

Concomitants: Compound role

RAD

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Binding energy of the compound was determined by using the equation deltaG obsd = -RT ln KD

Substance RN

774694View in Reaxys

Qualitative Results

Binding energy not calculated

Measurement Parameter

qualitative

Measurement Object

Binding Energy

Andrews; Craik; Martin; Journal of Medicinal Chemistry; vol. 27; nb. 12; (1984); p. 1648 - 1657, View in Reaxys 105 of 189

Target Name

4-aminobutyrate aminotransferase, mitochondrial [rat]

Target Synonyms

(s)-3-amino-2-methylpropionate transaminase; 4-aminobutyrate aminotransferase, mitochondrial; abat; gaba aminotransferase; gaba transaminase; gaba-at; gaba-t; gabat; gamma-amino-n-butyrate transaminase; l-aibat

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

59/94

2018-08-03 20:32:29

106 of 189

Target Uniprot ID

p50554

Target, Subunit, Species

4-aminobutyrate aminotransferase, mitochondrial [rat]

Target Mutant/Chimera Details

4-aminobutyrate aminotransferase, mitochondrial [rat]:Wild

Target Species (Bioactivity)

rat

Substance Action on Target

Inhibitor

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Percent inhibition of Gamma-amino-N-butyrate transaminase in rat diencephalon membranes upon incubation with0.2 M Tris-Hcl buffer of pH 8.0 at 37 degree C for 30 min; ND = no data

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Qualitative Results

ND

Measurement Parameter

Activity

Measurement Object

Inhibition

Qualitative value

ND

Target Name

Gamma-aminobutyric acid receptor subunit delta [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat];+Gamma-aminobutyric acid receptor subunit alpha-1 [rat]

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p18506 + p63138 + p62813

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit delta [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Wild

Target Species (Bioactivity)

rat

Substance Action on Target

Inhibitor

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

In vitro inhibitory activity against gamma-aminobutyric-acid receptor in rat brain synaptic membranes using [3H]GABA (0.005 uM) as radioligand upon incubation for 15 min at 4 degree C in 0.05 M Tris-citrate buffer (pH 7.1)

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Measurement Parameter

IC50

Unit

ÂľM

Qualitative value

=

Quantitative value

0.006

Measurement pX

8.22

Concomitants: Compound name

[3H]GABA

Concomitants: Compound role

RAD

Krogsgaard-Larsen; Nielsen; Falch; Curtis; Journal of Medicinal Chemistry; vol. 28; nb. 11; (1985); p. 1612 - 1617, View in Reaxys

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

60/94

2018-08-03 20:32:29

107 of 189

108 of 189

109 of 189

Target Name

Gamma-aminobutyric acid receptor subunit delta;+Gamma-aminobutyric acid receptor subunit beta-2;+Gamma-aminobutyric acid receptor subunit alpha-1

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit delta

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta:Wild + Gamma-aminobutyric acid receptor subunit beta-2:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild

Substance Action on Target

Inhibitor

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Inhibitory activity of the compound against GABAA receptor

Substance RN

774694View in Reaxys

Measurement Parameter

Ki (inhibition constant)

Unit

nM

Qualitative value

=

Quantitative value

2.8

Measurement pX

8.55

Target Name

Gamma-aminobutyric acid receptor subunit delta [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat];+Gamma-aminobutyric acid receptor subunit alpha-1 [rat]

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p18506 + p63138 + p62813

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit delta [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Wild

Target Species (Bioactivity)

rat

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

In vitro Binding affinity of the compound towards GABAA receptor isolated from rat brain membrane using [3H]Muscimol as radioligand

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Measurement Parameter

IC50

Unit

ÂľM

Qualitative value

=

Quantitative value

0.01

Measurement pX

8

Concomitants: Compound name

[3H]Muscimol

Concomitants: Compound role

RAD

Target Name

Gamma-aminobutyric acid receptor subunit delta [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat];+Gamma-aminobutyric acid receptor subunit alpha-1 [rat]

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

61/94

2018-08-03 20:32:29

110 of 189

111 of 189

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p18506 + p63138 + p62813

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit delta [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Wild

Target Species (Bioactivity)

rat

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Binding affinity of the compound towards GABAA receptor by the displacement of [3H]GABA from rat brain membranes in 50 mM tris-citrate buffer of pH 7.1 upon incubation for 30 min at 37 degree C

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Measurement Parameter

IC50

Unit

ÂľM

Qualitative value

=

Quantitative value

0.01

Measurement pX

8

Concomitants: Compound name

[3H]GABA

Concomitants: Compound role

RAD

Target Name

Gamma-aminobutyric acid receptor subunit delta;+Gamma-aminobutyric acid receptor subunit beta-2;+Gamma-aminobutyric acid receptor subunit alpha-1

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit delta

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta:Wild + Gamma-aminobutyric acid receptor subunit beta-2:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild

Substance Action on Target

Inhibitor

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Inhibitory concentration against GABAA receptor

Substance RN

774694View in Reaxys

Measurement Parameter

IC50

Unit

nM

Qualitative value

=

Quantitative value

7.2

Measurement pX

8.14

Bioassay Category

In Vivo (Animal models)

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

62/94

2018-08-03 20:32:29

Bioassay Details

Ability of the compound to antagonize mercaptopropionic acid (32 mg/kg, intraperitoneally) induced convulsions in mouse for 10 min after administration of compound at 100 mg/kg subcutaneously

Biological Species/NCBI ID

mouse

Substance RN

774694View in Reaxys

Substance Route of Adm.

oral administration

Measurement Parameter

ED50

Unit

mg/kg

Qualitative value

=

Quantitative value

0.7

Sarges; Howard; Koe; Weissman; Journal of Medicinal Chemistry; vol. 32; nb. 2; (1989); p. 437 - 444, View in Reaxys 112 of 189

Bioassay Category

In Vivo (Animal models)

Bioassay Details

Ability to antagonize isoniazid (450 mg/kg, sc) induced cyclic GMP accumulation in mouse brain after i. p. administration of compound

Biological Species/NCBI ID

mouse

Organs/Tissues

brain

Substance RN

774694View in Reaxys

Substance Route of Adm.

intraperitoneal administration

Measurement Parameter

ED50

Unit

mg/kg

Qualitative value

=

Quantitative value

1.5

Sarges; Howard; Koe; Weissman; Journal of Medicinal Chemistry; vol. 32; nb. 2; (1989); p. 437 - 444, View in Reaxys 113 of 189

Bioassay Category

Toxicity/Safety Pharmacology

Bioassay Details

Toxicity of the compound was measured in mouse by subcutaneous administration

Biological Species/NCBI ID

mouse

Substance RN

774694View in Reaxys

Substance Route of Adm.

subcutaneous administration

Measurement Parameter

LD50

Unit

mg/kg

Measurement Object

Toxicity

Qualitative value

=

Quantitative value

4.2

Sarges; Howard; Koe; Weissman; Journal of Medicinal Chemistry; vol. 32; nb. 2; (1989); p. 437 - 444, View in Reaxys 114 of 189

Bioassay Category

Toxicity/Safety Pharmacology

Bioassay Details

Toxicity of the compound was measured in mouse by intraperitoneal administration; LD50 range = 3.2-10 mg/kg

Biological Species/NCBI ID

mouse

Substance RN

774694View in Reaxys

Substance Route of Adm.

intraperitoneal administration

Measurement Parameter

LD50

Unit

mg/kg

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

63/94

2018-08-03 20:32:29

Qualitative value

=

Quantitative value

10

Sarges; Howard; Koe; Weissman; Journal of Medicinal Chemistry; vol. 32; nb. 2; (1989); p. 437 - 444, View in Reaxys 115 of 189

Target Name

Gamma-aminobutyric acid receptor subunit delta [mouse];+Gamma-aminobutyric acid receptor subunit beta-2 [mouse];+Gamma-aminobutyric acid receptor subunit alpha-1 [mouse]

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p22933 + p63137 + p62812

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [mouse]; Gamma-aminobutyric acid receptor subunit beta-2 [mouse]; Gamma-aminobutyric acid receptor subunit delta [mouse]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta [mouse]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [mouse]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [mouse]:Wild

Target Species (Bioactivity)

mouse

Bioassay Category

In Vivo (Animal models)

Bioassay Details

In vivo binding of [3H]Flunitrazepam (200 uCi/kg, iv) binding to Gamma-aminobutyric acid receptor in mouse brain when compound administered at a concentration of 320 umol/kg, s. c. 60 min prior to radioligand

Biological Species/NCBI ID

mouse

Organs/Tissues

brain

Substance RN

774694View in Reaxys

Substance Dose

= 320 Âľmol/kg

Substance Route of Adm.

subcutaneous administration

Measurement Parameter

percentage

Unit

%

Measurement Object

[3H]FNP bound

Qualitative value

=

Quantitative value

124

Deviation

6

Concomitants: Compound name

[3H]Flunitrazepam

Concomitants: Compound role

RAD

Sarges; Howard; Koe; Weissman; Journal of Medicinal Chemistry; vol. 32; nb. 2; (1989); p. 437 - 444, View in Reaxys 116 of 189

Target Name

Gamma-aminobutyric acid receptor subunit delta [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

o14764 + p47870 + p14867

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit delta [human]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild

Target Species (Bioactivity)

human

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

64/94

2018-08-03 20:32:29

117 of 189

118 of 189

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Effective concentration of the compound required to measure membrane potentials in tsA201 cells expressing human GAMMA-AMINOBUTYRIC ACID A RECEPTOR RHO1 upon incubation for 30 minutes at 37 degree C determined by FLIPR MEMBRANE POTENTIAL (FMP) BLUE ASSAY

Biological Species/NCBI ID

human

Cells/Cell Lines

tsA201 cell line

Substance RN

774694View in Reaxys

Measurement Parameter

EC50

Unit

µM

Measurement Object

EC50

Quantitative value

0.54

Measurement pX

6.27

Target Name

Gamma-aminobutyric acid receptor subunit delta [rat];+Gamma-aminobutyric acid receptor subunit beta-2 [rat];+Gamma-aminobutyric acid receptor subunit alpha-1 [rat]

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p18506 + p63138 + p62813

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [rat]; Gamma-aminobutyric acid receptor subunit beta-2 [rat]; Gamma-aminobutyric acid receptor subunit delta [rat]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta [rat]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [rat]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [rat]:Wild

Target Species (Bioactivity)

rat

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Binding affinity of compound towards GAMMA-AMINOBUTYRIC ACID (GABA) A RECEPTOR in rat brain synaptic membranes of cortex and the central hemispheres using 5 nM [3H]muscimol radio ligand upon incubation for 60 minutes at 0 degree C

Biological Species/NCBI ID

rat

Substance RN

774694View in Reaxys

Measurement Parameter

Ki (inhibition constant)

Unit

µM

Qualitative value

=

Quantitative value

0.007

Measurement pX

8.15

Concomitants: Compound name

[3H]MUSCIMOL

Concomitants: Compound role

RAD

Target Name

Gamma-aminobutyric acid receptor subunit delta [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

o14764 + p47870 + p14867

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit delta [human]

Copyright © 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

65/94

2018-08-03 20:32:29

119 of 189

120 of 189

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild

Target Species (Bioactivity)

human

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Effective concentration of the compound required to measure membrane potentials in tsA201 cells expressing human GABA-A RECEPTOR ALPHA5BETA2GAMMA2S upon incubation for 30 minutes at 37 degree C determined by FLIPR MEMBRANE POTENTIAL (FMP) BLUE ASSAY

Biological Species/NCBI ID

human

Cells/Cell Lines

tsA201 cell line

Substance RN

774694View in Reaxys

Measurement Parameter

EC50

Unit

µM

Measurement Object

MEMBRANE POTENTIALS

Qualitative value

=

Quantitative value

0.054

Measurement pX

7.27

Target Name

Gamma-aminobutyric acid receptor subunit delta [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

o14764 + p47870 + p14867

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit delta [human]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild

Target Species (Bioactivity)

human

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Effective concentration of the compound required to measure membrane potentials in tsA201 cells expressing human GABAA RECEPTOR ALPHA2BETA2GAMMA2S upon incubation for 30 minutes at 37 degree C determined by FLIPR MEMBRANE POTENTIAL (FMP) BLUE ASSAY

Biological Species/NCBI ID

human

Cells/Cell Lines

tsA201 cell line

Substance RN

774694View in Reaxys

Measurement Parameter

EC50

Unit

µM

Measurement Object

MEMBRANE POTENTIALS

Qualitative value

=

Quantitative value

0.41

Measurement pX

6.39

Target Name

Gamma-aminobutyric acid receptor subunit delta [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]

Copyright © 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

66/94

2018-08-03 20:32:29

121 of 189

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

o14764 + p47870 + p14867

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit delta [human]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild

Target Species (Bioactivity)

human

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Effective concentration of the compound required to measure membrane potentials in tsA201 cells expressing human GABAA RECEPTOR ALPHA3BETA2GAMMA2S upon incubation for 30 minutes at 37 degree C determined by FLIPR MEMBRANE POTENTIAL (FMP) BLUE ASSAY

Biological Species/NCBI ID

human

Cells/Cell Lines

tsA201 cell line

Substance RN

774694View in Reaxys

Measurement Parameter

EC50

Unit

µM

Measurement Object

MEMBRANE POTENTIALS

Qualitative value

=

Quantitative value

0.21

Measurement pX

6.68

Target Name

Gamma-aminobutyric acid receptor subunit delta [human];+Gamma-aminobutyric acid receptor subunit beta-2 [human];+Gamma-aminobutyric acid receptor subunit alpha-1 [human]

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

o14764 + p47870 + p14867

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [human]; Gamma-aminobutyric acid receptor subunit beta-2 [human]; Gamma-aminobutyric acid receptor subunit delta [human]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta [human]:Wild + Gamma-aminobutyric acid receptor subunit beta-2 [human]:Wild + Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild

Target Species (Bioactivity)

human

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Effective concentration of the compound required to measure membrane potentials in tsA201 cells expressing human GAMMA-AMINOBUTYRIC ACID A RECEPTOR RHO1 upon incubation for 30 minutes at 37 degree C determined by FLIPR MEMBRANE POTENTIAL (FMP) BLUE ASSAY

Biological Species/NCBI ID

human

Cells/Cell Lines

tsA201 cell line

Substance RN

774694View in Reaxys

Measurement Parameter

EC50

Unit

µM

Measurement Object

EC50

Copyright © 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

67/94

2018-08-03 20:32:29

122 of 189

123 of 189

124 of 189

Quantitative value

0.7

Measurement pX

6.15

Target Name

adenosine receptor a3 [human]

Target Synonyms

adenosine receptor a3; adora3

Target Uniprot ID

p0dms8

Target PDB ID

1oea; 1r7n

Target, Subunit, Species

adenosine receptor a3 [human]

Target Mutant/Chimera Details

adenosine receptor a3 [human]:Wild

Target Species (Bioactivity)

human

Substance Action on Target

Inhibitor

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Inhibitory concentration against human Adenosine A3 receptor expressed in HEK-293 cells upon incubation at 22 degree C for 90 min and pH 7.4 using (0.1 nM) [3H]AB-MECA

Biological Species/NCBI ID

human

Cells/Cell Lines

HEK293 cell line

Substance RN

774694View in Reaxys

Measurement Parameter

IC50

Unit

nM

Qualitative value

=

Quantitative value

16

Measurement pX

7.8

Concomitants: Compound name

[3H]AB-MECA

Concomitants: Compound role

RAD

Target Name

Gamma-aminobutyric acid receptor subunit delta;+Gamma-aminobutyric acid receptor subunit beta-2;+Gamma-aminobutyric acid receptor subunit alpha-1

Target Synonyms

gaba(a) receptor subunit delta; gabrd; gamma-aminobutyric acid receptor subunit delta + gaba(a) receptor subunit beta-2; gabrb-2; gabrb2; gamma-aminobutyric acid receptor subunit beta-2 + gaba(a) receptor subunit alpha-1; gabra-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1; Gamma-aminobutyric acid receptor subunit beta-2; Gamma-aminobutyric acid receptor subunit delta

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit delta:Wild + Gamma-aminobutyric acid receptor subunit beta-2:Wild + Gamma-aminobutyric acid receptor subunit alpha-1:Wild

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Binding activity of the compound towards GABAA receptor in mammalian tissue; Active

Substance RN

774694View in Reaxys

Measurement Parameter

Activity

Qualitative value

ND

Substance Action on Target

Inhibitor

Bioassay Category

In Vitro (Efficacy)

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

68/94

2018-08-03 20:32:29

Bioassay Details

The compound was tested for its ability to inhibit GABA receptor evoked release of 3H-GABA; Active

Substance RN

774694View in Reaxys

Qualitative Results

Acitve

Measurement Parameter

Activity

Qualitative value

@

Pieter B. M. W. M. Tiumermans; Annual reports in medicinal chemistry; vol. 19; (1984); p. 51 - 60, View in Reaxys 125 of 189

126 of 189

127 of 189

Target Name

chloride channel

Target Synonyms

chloride channel; chloride channel activity

Target, Subunit, Species

chloride channel

Target Mutant/Chimera Details

chloride channel:Wild

Bioassay Category

In Vitro (Efficacy)

Bioassay Details

Percent response (agonist) elicited with 1 mM of glutamate mediated by glutamate gated chloride channels; NR=no response

Substance RN

774694View in Reaxys

Qualitative Results

NR

Measurement Parameter

Activity

Qualitative value

@

Target Name

GABA-A receptor

Target Synonyms

gaba-a receptor; gaba-a receptor activity; ionotropic gaba receptor

Target, Subunit, Species

GABA-A receptor

Target Mutant/Chimera Details

GABA-A receptor:Wild

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Cells/Cell Lines

CHO-K1 cell line

Cell Fraction

Membrane: homogenate

Substance RN

774694View in Reaxys

Substance Name

muscimol

Measurement Parameter

inhibition rate

Unit

%

Quantitative value

93.53

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Substance Action on Target

Inhibitor

Bioassay Category

In Vitro (Efficacy)

Substance RN

774694View in Reaxys

Substance Name

muscimol

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

69/94

2018-08-03 20:32:29

128 of 189

Substance Dose

10 μM

Measurement Parameter

inhibition rate

Unit

%

Quantitative value

100

Measurement pX

6.28

Concomitants: Compound RN

8177147

Concomitants: Compound role

RAD

Substance Effect

Antiulcerative

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Swiss Webster mouse

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.1 mg/kg

Substance Route of Adm.

intraperitoneal administration

Substance Dosing Regimen

Single

Measurement Parameter

inhibition rate

Measurement Object

GASTRIC LESION AREA

Qualitative value

Active

Statistical sgnificance (Qualitative)

S

Viana, Ana Flávia Seraine Custódio; da Silva, Francilene Vieira; Fernandes, Hélio de Barros; Oliveira, Irisdalva Sousa; Braga, Milena Aguiar; Nunes, Paulo Iury Gomes; Viana, Daniel de Araújo; de Sousa, Damião Pergentino; Rao, Vietla Satyanarayana; Oliveira, Rita de Cássia Meneses; Almeida Santos, Flávia; Journal of Pharmacy and Pharmacology; (2016); p. 1085 - 1092, View in Reaxys 129 of 189

Target Name

GABA-A receptor [Swiss Webster mouse]

Target Synonyms

gaba-a receptor

Target, Subunit, Species

GABA-A receptor [Swiss Webster mouse]

Target Mutant/Chimera Details

GABA-A receptor [Swiss Webster mouse]:Wild

Target Species (Bioactivity)

Swiss Webster mouse

Target Transfection

Non Transfected

Substance Effect

Antiulcerative

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Swiss Webster mouse

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.1 mg/kg

Substance Route of Adm.

intraperitoneal administration

Substance Dosing Regimen

Single

Measurement Parameter

inhibition rate

Copyright © 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

70/94

2018-08-03 20:32:29

Measurement Object

GASTRIC LESION AREA

Qualitative value

Not active

Measurement pX

1

Concomitants: Compound RN

4763661

Concomitants: Compound name

flumazenil

Concomitants: Compound role

ANT

Viana, Ana Flávia Seraine Custódio; da Silva, Francilene Vieira; Fernandes, Hélio de Barros; Oliveira, Irisdalva Sousa; Braga, Milena Aguiar; Nunes, Paulo Iury Gomes; Viana, Daniel de Araújo; de Sousa, Damião Pergentino; Rao, Vietla Satyanarayana; Oliveira, Rita de Cássia Meneses; Almeida Santos, Flávia; Journal of Pharmacy and Pharmacology; (2016); p. 1085 - 1092, View in Reaxys 130 of 189

Substance Effect

Antiulcerative

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Swiss Webster mouse

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

20 mg/kg

Substance Route of Adm.

intraperitoneal administration

Substance Dosing Regimen

Single

Measurement Parameter

inhibition rate

Measurement Object

GASTRIC LESION AREA

Qualitative value

Not active

Measurement pX

1

Concomitants: Compound RN

1714622

Concomitants: Compound name

NG-nitro-L-arginine methyl ester

Concomitants: Compound role

COM

Viana, Ana Flávia Seraine Custódio; da Silva, Francilene Vieira; Fernandes, Hélio de Barros; Oliveira, Irisdalva Sousa; Braga, Milena Aguiar; Nunes, Paulo Iury Gomes; Viana, Daniel de Araújo; de Sousa, Damião Pergentino; Rao, Vietla Satyanarayana; Oliveira, Rita de Cássia Meneses; Almeida Santos, Flávia; Journal of Pharmacy and Pharmacology; (2016); p. 1085 - 1092, View in Reaxys 131 of 189

Target Name

Gamma-aminobutyric acid receptor subunit alpha-1 [human]

Target Synonyms

gaba(a) receptor subunit alpha-1; gabra1; gamma-aminobutyric acid receptor subunit alpha-1

Target Uniprot ID

p14867

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-1 [human]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit alpha-1 [human]:Wild

Target Species (Bioactivity)

human

Target Transfection

Transfected

Substance Action on Target

Agonist

Bioassay Category

In Vitro (Efficacy)

Copyright © 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

71/94

2018-08-03 20:32:29

132 of 189

133 of 189

Cells/Cell Lines

CHO cell line

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

3 nM - 10 μM

Measurement Parameter

EC50

Unit

μM

Quantitative value

4

Measurement pX

5.4

Concomitants: Compound RN

98786

Concomitants: Compound name

bicuculline

Concomitants: Compound role

ANT

Target Name

Gamma-aminobutyric acid receptor subunit alpha-2 [human]

Target Synonyms

gaba(a) receptor subunit alpha-2; gabra2; gamma-aminobutyric acid receptor subunit alpha-2

Target Uniprot ID

p47869

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-2 [human]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit alpha-2 [human]:Wild

Target Species (Bioactivity)

human

Target Transfection

Transfected

Substance Action on Target

Agonist

Bioassay Category

In Vitro (Efficacy)

Cells/Cell Lines

CHO cell line

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

3 nM - 10 μM

Measurement Parameter

EC50

Unit

nM

Quantitative value

675

Measurement pX

6.17

Concomitants: Compound RN

98786

Concomitants: Compound name

bicuculline

Concomitants: Compound role

ANT

Target Name

Gamma-aminobutyric acid receptor subunit alpha-3 [human]

Target Synonyms

gaba(a) receptor subunit alpha-3; gabra3; gamma-aminobutyric acid receptor subunit alpha-3

Target Uniprot ID

p34903

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-3 [human]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit alpha-3 [human]:Wild

Copyright © 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

72/94

2018-08-03 20:32:29

134 of 189

135 of 189

Target Species (Bioactivity)

human

Target Transfection

Transfected

Substance Action on Target

Agonist

Bioassay Category

In Vitro (Efficacy)

Cells/Cell Lines

CHO cell line

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

3 nM - 10 μM

Measurement Parameter

EC50

Unit

nM

Quantitative value

367

Measurement pX

6.44

Concomitants: Compound RN

98786

Concomitants: Compound name

bicuculline

Concomitants: Compound role

ANT

Target Name

Gamma-aminobutyric acid receptor subunit alpha-5 [human]

Target Synonyms

gaba(a) receptor subunit alpha-5; gabra5; gamma-aminobutyric acid receptor subunit alpha-5

Target Uniprot ID

p31644

Target, Subunit, Species

Gamma-aminobutyric acid receptor subunit alpha-5 [human]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor subunit alpha-5 [human]:Wild

Target Species (Bioactivity)

human

Target Transfection

Transfected

Substance Action on Target

Agonist

Bioassay Category

In Vitro (Efficacy)

Cells/Cell Lines

CHO cell line

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

3 nM - 10 μM

Measurement Parameter

EC50

Unit

nM

Quantitative value

80

Measurement pX

7.1

Concomitants: Compound RN

98786

Concomitants: Compound name

bicuculline

Concomitants: Compound role

ANT

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Copyright © 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

73/94

2018-08-03 20:32:29

136 of 189

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Effect

Orexigenic

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Sprague Dawley rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

50 ng

Substance Route of Adm.

intracerebroventricular administration

Measurement Parameter

% Increase

Measurement Object

in satiated state animals

Qualitative value

Active

Statistical sgnificance (Qualitative)

S

Concomitants: Compound RN

97276

Concomitants: Compound name

yohimbine

Concomitants: Compound role

COM

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Effect

Orexigenic

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Sprague Dawley rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

100 ng

Substance Route of Adm.

intracerebroventricular administration

Measurement Parameter

% Increase

Unit

%

Measurement Object

in satiated state animals

Quantitative value

101

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

74/94

2018-08-03 20:32:29

137 of 189

138 of 189

139 of 189

Statistical sgnificance (Qualitative)

S

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Effect

Orexigenic

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Sprague Dawley rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

50 ng

Substance Route of Adm.

intracerebroventricular administration

Measurement Parameter

% Increase

Measurement Object

in satiated state animals

Qualitative value

Active

Statistical sgnificance (Qualitative)

S

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Effect

Orexigenic

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Sprague Dawley rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

25 ng

Substance Route of Adm.

intracerebroventricular administration

Measurement Parameter

% Increase

Measurement Object

in satiated state animals

Qualitative value

Not active

Measurement pX

1

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

75/94

2018-08-03 20:32:29

140 of 189

141 of 189

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Effect

Orexigenic

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Sprague Dawley rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

25 ng

Substance Route of Adm.

intracerebroventricular administration

Measurement Parameter

% Increase

Measurement Object

in satiated state animals

Qualitative value

Active

Statistical sgnificance (Qualitative)

S

Concomitants: Compound RN

883415

Concomitants: Compound name

clonidine

Concomitants: Compound role

COM

Target Name

Gamma-aminobutyric acid receptor lgc-35 [Caenorhabditis elegans]

Target Synonyms

gamma-aminobutyric acid receptor lgc-35

Target, Subunit, Species

Gamma-aminobutyric acid receptor lgc-35 [Caenorhabditis elegans]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor lgc-35 [Caenorhabditis elegans]:Wild

Target Species (Bioactivity)

Caenorhabditis elegans

Target Transfection

Transfected

Substance Action on Target

Agonist

Bioassay Category

In Vitro (Efficacy)

Biological Species/NCBI ID

Xenopus laevis

Cells/Cell Lines

oocyte

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

1-3000 ÎźM

Measurement Parameter

nH (Hill coefficient)

Unit

no unit

Quantitative value

2.1

Target Name

Gamma-aminobutyric acid receptor exp-1 [Caenorhabditis elegans]

Target Synonyms

exp-1; expulsion defective protein 3; gaba(a) receptor; gamma-aminobutyric acid receptor exp-1

Target Uniprot ID

g5ecj0

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

76/94

2018-08-03 20:32:29

142 of 189

143 of 189

Target, Subunit, Species

Gamma-aminobutyric acid receptor exp-1 [Caenorhabditis elegans]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor exp-1 [Caenorhabditis elegans]:Wild

Target Species (Bioactivity)

Caenorhabditis elegans

Target Transfection

Transfected

Substance Action on Target

Agonist

Bioassay Category

In Vitro (Efficacy)

Biological Species/NCBI ID

Xenopus laevis

Cells/Cell Lines

oocyte

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

1-3000 μM

Measurement Parameter

EC50

Unit

μM

Measurement Object

Current amplitude

Quantitative value

250

Measurement pX

3.6

Target Name

Gamma-aminobutyric acid receptor exp-1 [Caenorhabditis elegans]

Target Synonyms

exp-1; expulsion defective protein 3; gaba(a) receptor; gamma-aminobutyric acid receptor exp-1

Target Uniprot ID

g5ecj0

Target, Subunit, Species

Gamma-aminobutyric acid receptor exp-1 [Caenorhabditis elegans]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor exp-1 [Caenorhabditis elegans]:Wild

Target Species (Bioactivity)

Caenorhabditis elegans

Target Transfection

Transfected

Substance Action on Target

Agonist

Bioassay Category

In Vitro (Efficacy)

Biological Species/NCBI ID

Xenopus laevis

Cells/Cell Lines

oocyte

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

1-3000 μM

Measurement Parameter

nH (Hill coefficient)

Unit

no unit

Quantitative value

1.2

Target Name

Gamma-aminobutyric acid receptor lgc-35 [Caenorhabditis elegans]

Target Synonyms

gamma-aminobutyric acid receptor lgc-35

Target, Subunit, Species

Gamma-aminobutyric acid receptor lgc-35 [Caenorhabditis elegans]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor lgc-35 [Caenorhabditis elegans]:Wild

Copyright © 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

77/94

2018-08-03 20:32:29

144 of 189

145 of 189

Target Species (Bioactivity)

Caenorhabditis elegans

Target Transfection

Transfected

Substance Action on Target

Agonist

Bioassay Category

In Vitro (Efficacy)

Biological Species/NCBI ID

Xenopus laevis

Cells/Cell Lines

oocyte

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

1-3000 μM

Measurement Parameter

EC50

Unit

μM

Measurement Object

Current amplitude

Quantitative value

348

Measurement pX

3.46

Target Name

Gamma-aminobutyric acid receptor exp-1 [Caenorhabditis elegans]

Target Synonyms

exp-1; expulsion defective protein 3; gaba(a) receptor; gamma-aminobutyric acid receptor exp-1

Target Uniprot ID

g5ecj0

Target, Subunit, Species

Gamma-aminobutyric acid receptor exp-1 [Caenorhabditis elegans]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor exp-1 [Caenorhabditis elegans]:Wild

Target Species (Bioactivity)

Caenorhabditis elegans

Target Transfection

Transfected

Substance Action on Target

Agonist

Bioassay Category

In Vitro (Efficacy)

Biological Species/NCBI ID

Xenopus laevis

Cells/Cell Lines

oocyte

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

1-3000 μM

Measurement Parameter

Emax

Unit

%

Measurement Object

Current amplitude

Quantitative value

79

Target Name

Gamma-aminobutyric acid receptor lgc-35 [Caenorhabditis elegans]

Target Synonyms

gamma-aminobutyric acid receptor lgc-35

Target, Subunit, Species

Gamma-aminobutyric acid receptor lgc-35 [Caenorhabditis elegans]

Target Mutant/Chimera Details

Gamma-aminobutyric acid receptor lgc-35 [Caenorhabditis elegans]:Wild

Target Species (Bioactivity)

Caenorhabditis elegans

Copyright © 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

78/94

2018-08-03 20:32:29

146 of 189

147 of 189

Target Transfection

Transfected

Substance Action on Target

Agonist

Bioassay Category

In Vitro (Efficacy)

Biological Species/NCBI ID

Xenopus laevis

Cells/Cell Lines

oocyte

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

1-3000 ÎźM

Measurement Parameter

Emax

Unit

%

Measurement Object

Current amplitude

Quantitative value

62

Target Name

ionotropic gamma aminobutyric acid a receptor [rat]

Target Synonyms

ionotropic gamma aminobutyric acid a receptor

Target, Subunit, Species

ionotropic gamma aminobutyric acid a receptor [rat]

Target Mutant/Chimera Details

ionotropic gamma aminobutyric acid a receptor [rat]:Wild

Target Species (Bioactivity)

rat

Target Transfection

Non Transfected

Substance Action on Target

Radioligand (/ligand)

Bioassay Category

In Vitro (Efficacy)

Biological Species/NCBI ID

rat

Organs/Tissues

cerebellum

Substance RN

774694View in Reaxys

Measurement Parameter

inhibition rate

Unit

%

Quantitative value

97.89

Concomitants: Compound RN

774694

Concomitants: Compound role

RAD

Substance Effect

Antiaddictive

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

1-4 mg/kg

Substance Route of Adm.

intraperitoneal administration

Substance Dosing Regimen

Single

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

79/94

2018-08-03 20:32:29

Measurement Parameter

% Decrease

Measurement Object

cocaine self administration

Qualitative value

Active

Concomitants: Compound RN

91034

Concomitants: Compound name

cocaine

Concomitants: Compound role

IND

Patent; EMBERA NEUROTHERAPEUTICS, INC.; DETKE, Michael; GLOFF, Carol; STRAUB, Julie; (80 pag.); WO2017/143034; (2017); (A1) English, View in Reaxys 148 of 189

Substance Effect

Antiaddictive

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.5-2 mg/kg

Substance Route of Adm.

intraperitoneal administration

Substance Dosing Regimen

Single

Measurement Parameter

% Decrease

Measurement Object

cocaine self administration

Qualitative value

Not active

Measurement pX

1

Concomitants: Compound RN

91034

Concomitants: Compound name

cocaine

Concomitants: Compound role

IND

Patent; EMBERA NEUROTHERAPEUTICS, INC.; DETKE, Michael; GLOFF, Carol; STRAUB, Julie; (80 pag.); WO2017/143034; (2017); (A1) English, View in Reaxys 149 of 189

Substance Effect

Toxic

Bioassay Category

Toxicity/Safety Pharmacology

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

1-4 mg/kg

Substance Route of Adm.

intraperitoneal administration

Substance Dosing Regimen

Single

Measurement Parameter

% Change

Measurement Object

food maintained responding

Qualitative value

Not active

Measurement pX

1

Copyright © 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

80/94

2018-08-03 20:32:29

Concomitants: Compound RN

91034

Concomitants: Compound name

cocaine

Concomitants: Compound role

IND

Patent; EMBERA NEUROTHERAPEUTICS, INC.; DETKE, Michael; GLOFF, Carol; STRAUB, Julie; (80 pag.); WO2017/143034; (2017); (A1) English, View in Reaxys 150 of 189

Substance Effect

Toxic

Bioassay Category

Toxicity/Safety Pharmacology

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.5-2 mg/kg

Substance Route of Adm.

intraperitoneal administration

Substance Dosing Regimen

Single

Measurement Parameter

% Change

Measurement Object

food maintained responding

Qualitative value

Not active

Measurement pX

1

Concomitants: Compound RN

91034

Concomitants: Compound name

cocaine

Concomitants: Compound role

IND

Patent; EMBERA NEUROTHERAPEUTICS, INC.; DETKE, Michael; GLOFF, Carol; STRAUB, Julie; (80 pag.); WO2017/143034; (2017); (A1) English, View in Reaxys 151 of 189

Substance Effect

Anti-Alzheimer

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.05 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

escape latency decrease

Measurement Object

mean escape latency

Qualitative value

Active

Statistical sgnificance (Qualitative)

S

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 152 of 189

Substance Effect

Anti-Alzheimer

Bioassay Category

In Vivo (Animal models)

Copyright © 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

81/94

2018-08-03 20:32:29

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.01 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

escape latency decrease

Measurement Object

mean escape latency

Qualitative value

Active

Statistical sgnificance (Qualitative)

S

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 153 of 189

Substance Effect

Anti-Alzheimer

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.05 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% increase

Measurement Object

number of platform crossing

Qualitative value

Active

Statistical sgnificance (Qualitative)

S

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 154 of 189

Substance Effect

Anti-Alzheimer

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.01 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% increase

Measurement Object

number of platform crossing

Qualitative value

Active

Statistical sgnificance (Qualitative)

S

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 155 of 189

Substance Effect

Anti-Alzheimer

Bioassay Category

In Vivo (Animal models)

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

82/94

2018-08-03 20:32:29

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.01 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

time spent in target quadrant increase

Measurement Object

Time spent in the target quadrant

Qualitative value

Active

Statistical sgnificance (Qualitative)

S

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 156 of 189

Substance Effect

Anti-Alzheimer

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.05 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

time spent in target quadrant increase

Measurement Object

Time spent in the target quadrant

Qualitative value

Active

Statistical sgnificance (Qualitative)

S

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 157 of 189

Substance Effect

Anti-Alzheimer

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.05 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

swimming speed change

Measurement Object

Swimming speed

Qualitative value

Not active

Measurement pX

1

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 158 of 189

Substance Effect

Anti-Alzheimer

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Wistar rat

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

83/94

2018-08-03 20:32:29

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.01 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

swimming speed change

Measurement Object

Swimming speed

Qualitative value

Not active

Measurement pX

1

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 159 of 189

Substance Effect

Anti-Alzheimer

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.05 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% decrease

Qualitative value

Active

Statistical sgnificance (Qualitative)

S

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 160 of 189

Substance Effect

Anti-Alzheimer

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.01 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% decrease

Qualitative value

Active

Statistical sgnificance (Qualitative)

S

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 161 of 189

Substance Effect

Anti-Alzheimer

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.05 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

84/94

2018-08-03 20:32:29

Measurement Parameter

% decrease

Qualitative value

Active

Statistical sgnificance (Qualitative)

S

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 162 of 189

Substance Effect

Anti-Alzheimer

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.01 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% decrease

Qualitative value

Active

Statistical sgnificance (Qualitative)

S

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 163 of 189

Substance Effect

Anti-Alzheimer

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.05 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% decrease

Qualitative value

Active

Statistical sgnificance (Qualitative)

S

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 164 of 189

Substance Effect

Anti-Alzheimer

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.01 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% decrease

Qualitative value

Active

Statistical sgnificance (Qualitative)

S

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

85/94

2018-08-03 20:32:29

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 165 of 189

Substance Effect

Anti-Alzheimer

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.05 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% decrease

Qualitative value

Active

Statistical sgnificance (Qualitative)

S

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 166 of 189

Substance Effect

Anti-Alzheimer

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.01 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% decrease

Qualitative value

Active

Statistical sgnificance (Qualitative)

S

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 167 of 189

Substance Effect

Anti-Alzheimer

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.05 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% increase

Qualitative value

Active

Statistical sgnificance (Qualitative)

S

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 168 of 189

Substance Effect

Anti-Alzheimer

Bioassay Category

In Vivo (Animal models)

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

86/94

2018-08-03 20:32:29

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.01 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% increase

Qualitative value

Active

Statistical sgnificance (Qualitative)

S

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 169 of 189

Substance Effect

Anti-Alzheimer

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.05 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% increase

Qualitative value

Active

Statistical sgnificance (Qualitative)

S

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 170 of 189

Substance Effect

Anti-Alzheimer

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.01 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% increase

Qualitative value

Active

Statistical sgnificance (Qualitative)

S

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 171 of 189

Substance Effect

Toxic

Bioassay Category

Toxicity/Safety Pharmacology

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

87/94

2018-08-03 20:32:29

Substance Dose

0.05 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% change

Qualitative value

Not active

Measurement pX

1

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 172 of 189

Substance Effect

Toxic

Bioassay Category

Toxicity/Safety Pharmacology

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.01 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% change

Qualitative value

Not active

Measurement pX

1

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 173 of 189

Substance Effect

Toxic

Bioassay Category

Toxicity/Safety Pharmacology

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.05 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% change

Qualitative value

Not active

Measurement pX

1

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 174 of 189

Substance Effect

Toxic

Bioassay Category

Toxicity/Safety Pharmacology

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.01 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% change

Qualitative value

Not active

Measurement pX

1

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

88/94

2018-08-03 20:32:29

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 175 of 189

Substance Effect

Toxic

Bioassay Category

Toxicity/Safety Pharmacology

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.05 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% change

Qualitative value

Not active

Measurement pX

1

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 176 of 189

Substance Effect

Toxic

Bioassay Category

Toxicity/Safety Pharmacology

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.01 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% change

Qualitative value

Not active

Measurement pX

1

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 177 of 189

Substance Effect

Toxic

Bioassay Category

Toxicity/Safety Pharmacology

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.05 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% change

Qualitative value

Not active

Measurement pX

1

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 178 of 189

Substance Effect

Toxic

Bioassay Category

Toxicity/Safety Pharmacology

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

89/94

2018-08-03 20:32:29

Substance Name

muscimol

Substance Dose

0.01 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% change

Qualitative value

Not active

Measurement pX

1

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 179 of 189

Substance Effect

Toxic

Bioassay Category

Toxicity/Safety Pharmacology

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.05 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% change

Qualitative value

Not active

Measurement pX

1

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 180 of 189

Substance Effect

Toxic

Bioassay Category

Toxicity/Safety Pharmacology

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.01 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% change

Qualitative value

Not active

Measurement pX

1

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 181 of 189

Substance Effect

Toxic

Bioassay Category

Toxicity/Safety Pharmacology

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.05 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% change

Qualitative value

Not active

Measurement pX

1

Copyright Š 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

90/94

2018-08-03 20:32:29

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 182 of 189

Substance Effect

Toxic

Bioassay Category

Toxicity/Safety Pharmacology

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.01 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% change

Qualitative value

Not active

Measurement pX

1

Pilipenko, Vladimirs; Narbute, Karina; Beitnere, Ulrika; Rumaks, Juris; Pupure, Jolanta; Jansone, Baiba; Klusa, Vija; European Journal of Pharmacology; vol. 818; (2018); p. 381 - 399, View in Reaxys 183 of 189

Substance Effect

Antiaddictive

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

1- 4 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% decrease

Unit

%

Measurement Object

cocaine self-administration

Qualitative value

<

Quantitative value

50

Patent; EMBERA NEUROTHERAPEUTICS, INC.; DETKE, Michael; GLOFF, Carol; STRAUB, Julie; (87 pag.); WO2017/152022; (2017); (A1) English, View in Reaxys 184 of 189

Substance Effect

Antiaddictive

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.5- 2 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% Change

Unit

%

Qualitative value

<

Quantitative value

10

Patent; EMBERA NEUROTHERAPEUTICS, INC.; DETKE, Michael; GLOFF, Carol; STRAUB, Julie; (87 pag.); WO2017/152022; (2017); (A1) English, View in Reaxys 185 of 189

Bioassay Category

Toxicity/Safety Pharmacology

Copyright © 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

91/94

2018-08-03 20:32:29

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

1- 4 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% Change

Qualitative value

Not active

Measurement pX

1

Patent; EMBERA NEUROTHERAPEUTICS, INC.; DETKE, Michael; GLOFF, Carol; STRAUB, Julie; (87 pag.); WO2017/152022; (2017); (A1) English, View in Reaxys 186 of 189

Bioassay Category

Toxicity/Safety Pharmacology

Biological Species/NCBI ID

Wistar rat

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

0.5- 2 mg/kg

Substance Route of Adm.

intraperitoneal drug administration

Measurement Parameter

% Change

Qualitative value

Not active

Measurement pX

1

Patent; EMBERA NEUROTHERAPEUTICS, INC.; DETKE, Michael; GLOFF, Carol; STRAUB, Julie; (87 pag.); WO2017/152022; (2017); (A1) English, View in Reaxys 187 of 189

188 of 189

Target Name

GABAA receptor [zebra fish]

Target Synonyms

gabaa receptor

Target, Subunit, Species

GABAA receptor [zebra fish]

Target Mutant/Chimera Details

GABAA receptor [zebra fish]:Wild

Target Species (Bioactivity)

zebra fish

Target Transfection

Non Transfected

Bioassay Category

In Vivo (Animal models)

Biological Species/NCBI ID

zebra fish

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

1 - 1000 μM

Measurement Parameter

% change

Measurement Object

Activity

Qualitative value

Not active

Measurement pX

1

Target Name

GABA transporter 1

Target Synonyms

atgat1; bat1; bidirectional amino acid transporter 1; gaba transporter 1; gat1

Target, Subunit, Species

GABA transporter 1

Target Mutant/Chimera Details

GABA transporter 1:Wild

Copyright © 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

92/94

2018-08-03 20:32:29

189 of 189

Target Transfection

Transfected

Substance Action on Target

Inhibitor

Bioassay Category

In Vitro (Efficacy)

Biological Species/NCBI ID

mouse

Cells/Cell Lines

Brain cerebrocortical astrocyte

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

100 μM

Measurement Parameter

inhibition rate

Qualitative value

Not active

Measurement pX

1

Concomitants: Compound RN

906818

Concomitants: Compound name

4-aminobutyric acid

Concomitants: Compound role

SUB

Target Name

GABA transporter 1

Target Synonyms

atgat1; bat1; bidirectional amino acid transporter 1; gaba transporter 1; gat1

Target, Subunit, Species

GABA transporter 1

Target Mutant/Chimera Details

GABA transporter 1:Wild

Target Transfection

Transfected

Substance Action on Target

Inhibitor

Bioassay Category

In Vitro (Efficacy)

Biological Species/NCBI ID

mouse

Cells/Cell Lines

neuronal cell line

Substance RN

774694View in Reaxys

Substance Name

muscimol

Substance Dose

200 μM

Measurement Parameter

inhibition rate

Qualitative value

Not active

Measurement pX

1

Concomitants: Compound RN

906818

Concomitants: Compound name

4-aminobutyric acid

Concomitants: Compound role

SUB

Reaxys ID 14819161 View in Reaxys

Copyright © 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

2/2

93/94

2018-08-03 20:32:29

O

CAS Registry Number: 305808-13-3 Chemical Name: muscimol hydrate Linear Structure Formula: C4H6N2O2*H2O Molecular Formula: C4H6N2O2*H2O Molecular Weight: 132.119 InChI Key: QYGRQAWQDLPUDK-UHFFFAOYSA-N Note:

NH O

H 2O

NH 2

Druglikeness (1) 1 of 1

H Bond Donors

3

H Bond Acceptors

3

Rotatable Bonds

1

TPSA

64.35

Lipinski Number

3

Veber Number

2

Copyright © 2018 Elsevier Life Sciences IP Limited except certain content provided by third parties. Reaxys is a trademark of Elsevier Life Sciences IP Limited.

94/94

2018-08-03 20:32:29