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With GLUTATHIONE; water; sodium hydroxide in aq. phosphate buffer
T=20°C; pH=7.4; Sealed tube; Kinetics; Hide Experimental Procedure
Mohamed, Heba A.H.; Abdel-Aziz, Mohamed; Abuo-Rahma, Gamal El-Din A.A.; King, S. Bruce
Bioorganic and Medicinal Chemistry, 2015 , vol. 23, # 17 p. 6069 - 6077 Title/Abstract Full Text View citing articles Show Details
2.2.2. Kinetic UV–vis decomposition assay
General procedure: Phosphate buffered saline (PBS, 100 mM, pH 7.4, 1 mL) wasadded to a solution of 4 and 5 (60 lmol) in MeOH (1 mL) at rt ina sealed cuvette. UV–vis measurements were taken every 15 minfor 10 h at 667 nm. In other experiments, a solution of NaOH(0.1 M, 1 mL) was used and similar measurements made. Similarmeasurements were performed with the addition of PLE (200 lL,9 U/lmol substrate). Further experiments were performed in thepresence of thiols. A solution of GSH (70.2 lmol) in PBS(100 mM, pH = 7.4, 1 mL) was added to a solution of 4–7(35.1 lmol) in MeOH (1 mL) at rt in a sealed cuvette. UV–vis measurementswere taken every 1 min for 90 min at 665 nm. Similarexperiments were performed using another concentration of GSH(70.2 lmol) in NaOH (0.1 M, 1 mL). A
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