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Tissue distribution and excretion of deoxynivalenol in rainbow trout By Rui A. Gonçalves MSc, Scientist - Aquaculture, BIOMIN.
ADME mechanisms differ between animal species, the mechanisms specific to aquatic species should be studied. An experiment was therefore devised to assess the toxicokinetics of radiolabeled DON ([3H]-DON) in rainbow trout (Oncorhynchus mykiss), focusing on tissue distribution and excretion (Gonçalves et al., 2018).
Methodology
Fusarium mycotoxins are the most common mycotoxins found in aquafeeds, reflecting the type and inclusion levels of plant meals used in these diets. Deoxynivalenol (DON) is a metabolite produced by the genus Fusarium and the main mycotoxin found in small grain cereals.
The kinetics of DON absorption, distribution, metabolism and elimination (ADME) must be understood before it can be tackled in aquatic species. The toxic effects and toxicokinetics of DON have been comprehensively described in terrestrial farmed animals, but less is known in aquatic animals. As
Juvenile rainbow trout (Oncorhynchus mykiss) with a mean initial body weight of 7.72 ± 1.42 g were acclimatized in 40-liter cylindrical/conical fiberglass tanks for three weeks. During the acclimatization period, the fish were fed a mycotoxin-free diet at 1.5% of their body weight, four times a day by automatic feeders. Fish were kept in a freshwater recirculating system at 15 ± 1.0°C, with a 12 h light: 12 h dark photoperiod. Each feed pellet was labeled with 31.25 ng of the tracer, [3H]-DON
