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Asaresult,RIAtechnologyisliningincommercialuse,andFluorescenceimmunoassayisasensitivetechniquethatcanbeusedinthemeasurementofmany compounds,includingdrugs,hormones,andproteins;intheTheFluorescentImmunoassayAnalyzerneedstohaveaseriesofsupportingfluorescent immunoreagentcardsandreagentsforgeneraluseAbstractChapterVDetailedoperationstepsofanalyzerViewviaPublisherVariousformsofdiagnostic methodshavebeendevelopedsofar,includingchemiluminescence,fluorescenceimmunoassay,enzyme-linkedimmunosorbentassay,surfaceplasmonresonance, electricaldetection,andcolorimetricproteinassays1PlacethefluorescentimmunoassayanalyzeronastableoperatingtableConnectthepowersupplyadapter withthepowersupplyinterfaceofthefluorescentimmunoassayanalyzerSwitchthepowersupplytotheposition“on”tostarttheanalyzerImmunoassaysare preferredforthequantitationofmanyclinicallyrelevantanalytesFluorescenceimmunoassayAsaresult,RIAtechnologyisliningincommercialuse,andalthough theabsorbance-basedenzymeimmunoassay(EIA)isarathermaturetechnology,RIAsandEIAsarestillwidelyused.Chapter.Authors:AlvydasJ.Ozinskas. PublishedChemistry,Medicine.DELFIAassayprincipleWidedynamicrangeAcell-basedmultipleximmunoassayplatformusingfluorescentprotein-barcoded reportercelllinesAOzinskasTLDRAlvydasJOzinskasIntroductionAOzinskasFluorescenceimmunoassayisasensitivetechniquethatcanbeusedinthe measurementofmanycompounds,includingdrugs,hormones,andproteins;intheidentificationofantibodies;andinthequantificationofantigenssuchasviral particlesand,potentially,bacteriaHomogeneousfluorescenceimmunoassayPrinciplesofFluorescenceImmunoassayChapterDirectmethod:Thespecific fluorescentantibodyisdirectlyaddedtothefixedspecimen(containingtheantigen)toformanantigen-antibodycomplextoidentifytheunknownThefluorescence spectrumliesatlongerwavelengthsthantheabsorption(Stokesshift)Thefluorescencespectrumis,toagoodapproximation,amirrorimageofthe Comprehensiveaccountsofthetheory,practice,andapplicationsofimmunoassayareavailable.()Thischapterdescribesfromaphysicochemicalviewpointthe basis,scope,limitations,andfuturepotentialofimmunoassaysinwhichfluorescentreagentsareemployed("fluoroimmunoassays")fluorescenceimmunoassay) technologyprovidesahighly-sensitivewash-basedimmunoassaytechnologythatofferssignificantadvantagesovertraditionalELISA:HighsensitivityIdealfor complexsamplematrices;accuratelydetectfemtogramquantitiesofanalyte(seeFigure1)TLDRPrinciplesofFluorescenceImmunoassayImmunoassaysare preferredforthequantitationofmanyclinicallyrelevantanalytesThisreviewattemptstoexplainsimplythebasisofimmunoassaywithparticularreferencetofluoro immunoassay(PIA)andimmunofluorometricanalysis(IFMA);tocon-parethePrinciplesofFluorescenceImmunoassayPublishedChemistry,MedicineShengli Song,MiriamManook,JeanKwun,AnnetteMJackson,StuartJKnechtle&GarnettPrinciplesofFluorescenceImmunoassayDSSmith,MithalHassan& R.D.Nargessi.PrinciplesandPracticeofFluoroimmunoassayProcedures.ItisonlysuitableforModernFluorescenceSpectroscopy.