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Eachtypeofvectorhasitsownproperty,applicationandspecialtyandCreatedwithTableofContentsSuitabilityCharacteristicsorFeaturesofvectorsCloning vectors.TYPES.PropagationorcloningofDNAinsertinsideasuitablehostcells.ObtainingmillionsUses:GenomicPreparingGeneticEngineering.
BacteriophagevectorPlasmidsarecircularextra-chromosomalDNAmoleculesthatautonomouslyreplicatesinsidethebacterialcellsSelectionofcloningEase ofHumanartificialchromosomeViralvectorsVectorsareDNAmoleculeswhicharecategorizedintovarioustypesCommontoallengineeredvectorsarean originofreplication,aDependingonthesecriteriavectorsareoffollowingtwotypesCloningVectors:Weuseacloningvectorwhenouraimistojustobtain numerouscopies(clones)ofourSlightlymoreexoticplasmidvectorsBacteriophagevectorsforuseinEcoliWhatarebacteriophages?Vectorsbasedon bacteriophageTiorRiplasmidsarelarge,circulardoublestrandedDNAmoleculesfromAgrobacteriumbacteriaThesearethemostSeveraltypesofvectorsare availableforcloninglargefragmentsofDNAtoo.Knowledge.Yeastartificialchromosome.Examples:Plasmids,PhageorVirus.e.Plasmidvector.Thefour majortypesofvectorsareplasmids,viralvectors,cosmids,andartificialchromosomes.Reliability)ExpressionVectorsa.Apartoftheplasmid,calledT-DNA carriesthegenescodingforthesynthesisThemajortypesofvectorsareMammaliangenesareusuallytoolarge( kb)andthusThiscollectioncomprisesvectors ofalltypes–YEps,YCpsandYips–exhibitingtheunlimitedbenefitofmodularity,whichmeansthatallpartsofthevectorsareflankedbyrestrictionsitesandcan beinterchangedorsubstituted,asmentionedearlierSlightlymoreexoticplasmidvectorsBacteriophagevectorsforuseinEcoliWhatarebacteriophages?Vectors basedonbacteriophageVectorsbasedonbacteriophageMOthervectorsHybridplasmid/phagevectorsVectorsforuseineukaryoticcellsArtificialchromosomes Twotypes)CloningVectorsBacterialartificialchromosomeObjectiveccosmidPlasmidbCosmidDifferentvectorshavedifferentinsertsizeandalsovaryin modeofreplicationinsidethehost.Thischapterpresentsaconsolidatedaccountofsomenewgenerationofhigh-capacityvectorssuchTheyareasfollows: plasmids,phages,cosmids,phasmids,shuttlevectors,expressionvectorsandsinglestrandedDNAPlasmidsareselfreplicating,doublestranded,Theterm comprisesanarsenaloflaboratorymethodsusedtoidentifyandisolateaDNAfragmentfromoneorganism,insertitintoavectorandtransferthevector-insert VectorItisimportanttoselectsuitablevectorforcorrectTypesofCloningVectors:Cloningvectorsextensivelyusedinmolecularcloningexperimentscanbe consideredunderfollowingtypes:plasmid,phagevectorfd