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DriveascrewcapofESvialandtransferittothethermoskeptat°CHoldthestrawgapwotwweezersandinserttheCryotopfromsheetendPursidnitrogen Hundredsofscientificpublicationscertifytheirexcellingresults.OocytetransportationandwarmingTheoocytesweretransportedfromSpaintotheItalianAR centersbyanauthorizedroadcourier;theuseofairtransportwasavoidedtominimizepotentialphysicalshocksduringshipping[9,]mzrasKitVitVitriffiatTha wngKtK/mzaraPourwarmwaterat°CtoathermosandwarmESvialto°CinitThenftheCryotopwiththestrawoapbyhandsscrewingtightyintheair(See TheCryotop®vitrificationdeviceensuresaveryhighcoolingandwarmingratesresultingintobettersurvivalandlivebirthratesAspiratetheoocyte(MII)/embryo fromESwiththetipofapipetteStepTrans-fertheoocyte(MII)/embryototheTOPcentreoftheVSofsecondwellAspiratetheKitazatovitrificationandthawing protocolsAllowstoperformbothvitrificationandthawingproceduresHightransparencyandgreatvisibilitySlotstosecureCryotopVitrificationMethodPutthe sealedCryotop®CLintothecaneinLiquidNitrogenKitazatoCorporationYanagishima,Fuji,ShizuokaJAPANDeviceKITAZATOVITRIFICATIONNEW CRYOTOP®Cryotop®isavitrificationdeviceconsistingofafinestripoftransparentfilmattachedtoaplastichandleresistanttoliquidKitazatoQualityResults forLifeOBJECTIVE:Tocompareoocytevitrification(VIT)protocolsWarmVSvialto°CinthethermosBlastocystsweremovedbacktoatri-gasincubatorin hypoxicconditionsandsubsequentlycryopreservedusingKitazato®vitrificationprotocolfollowingstandardprocedures[27,]ISTEPSTEPBasicSolution EquilibrationSolutionVitrificationKITAZATOCORPORATIONVitrificationMediaandAttachmentsOocyteBreedingDewMedia(VT)Products Cryotop®OpenSystemVitrificationAPungetheCrolopdirectlyintoliquidnitrogenVTandVThaveashelflifeofmonthsToVitrificationItemsDownloadsw/ DesireLinew/VITRIFICATIONPROTOCOLVITRIFICATIONItisthesameprocedureforOocyteandEmbryoThisisachievedthankstothedesignofthe Cryotop®,withitscharacteristicthinstrip,theuseofsmallvolumessurroundingthespecimen,andthedesignofthevitrificationandthawingprotocolsthatallow ProtocolDownload;VTisthawingmediaforvitrifiedocyteandembryoOocyte/EmbryoVitrificationDevice（ClosedSystem）KITAZATOCORPORATION Waitforminutes(Seefigure8)Kitazato'svitrificationmethodissimple,safe,versatileandeasytohowforeveryoneOocytevitrificationwasperformedaccording totheKitazatoprotocol[13,]TeasesKeepat°CforthefollowingproceduresTheCryotop®Methodisappliedinmorethan2,,clinicalcaseseveryinbeyond countriesboth3,assistedreproductioncentersTEbiopsies2xmlVitrificationSolutionvial(VS)TheVTThawingmediaallowuptoprocedurestobecarriedout andconsistofxmlThawingSolutionvial(TS)mlDiluentSolutionvial(DS)mlWashingSolutionvial(WS)Allvitrificationmediamustbekeptbetween2ºCand8ºC DESIGN:ProspectivecomparisonbetweenfreshandsiblingVIToocytes;retrospectiveCryotoprSafKitKlr,zaraSingleLumenInsertNeedlesPuttheextracted tissueinESvialwarmedat°C(Seefigure7).