10. Antibodies for the detection of Tetanus toxin inhibitory impulses by interfering with the release of neurotransmitters. This leads to unopposed muscle contraction and spasm. Seizures may occur, and the autonomic nervous system may also be affected.
Tetanus toxin (tetanospasmin, TeNT) is the neurotoxin produced by the vegetative spore of Clostridium tetani in anaerobic conditions, causing tetanus – a medical condition that is characterized by a prolonged contraction of skeletal muscle fibers, which can eventually cause respiratory failure.
The peptide tetanospasmin has a molecular weight of 150kDa. It is made up of two parts: a 100 kDa heavy or B-chain and a 50 kDa light or A-chain. The chains are connected by a disulfide bond. The B-chain binds to disialogangliosides (GD2 and GD1b) on the neurone membrane. The A-chain, a zinc endopeptidase, attacks the vesicle-associated membrane protein. The chains are non-toxic after separation.
Tetanospasmin is one of the most potent toxins known: the estimated minimum human lethal dose is 2.5 nanograms per kilogram of body weight, or 175 nanograms in a 70 kg human. The toxin acts at several sites within the central nervous system, including peripheral nerve terminals, the spinal cord, and brain, and within the sympathetic nervous system. The clinical manifestations of tetanus are caused when tetanus toxin blocks
Using supernatant from C. tetani we managed to produce three monoclonal antibodies specific to tetanus toxin having toxin neutralization activity (in vivo assay) and suitable for EIA and WB applications.
Ordering information: Product
Cat. #
MAb
Isotype
Remarks
Anti-Tetanus Toxin Anti-Tetanus Toxin Anti-Tetanus Toxin
2TE8 2TE8 2TE8
TetE3 TetG2 TetH6
IgG1 IgG2a IgG1
EIA, WB EIA, WB EIA, WB
11. Antibodies for the detection of Aflatoxin from Aspergillus flavus Aspergillus flavus is a plant, animal, and human pathogen that produces the carcinogen, aflatoxin. Aflatoxins are potent toxic, carcinogenic, mutagenic and immunosuppressive agents, produced as secondary metabolites by the fungus Aspergillus flavus and A. parasiticus on variety of food products. Among 18 different types of aflatoxins identified, major members are aflatoxin B1, B2, G1 and G2. Aflatoxins M1 and M2 are major metabolites of aflatoxin
B1 and B2 respectively, found in milk of animals that have consumed feed contaminated with aflatoxins. We used aflatoxin from Aspergillus flavus conjugated to BSA as immunogen to produce monoclonal antibody against aflatoxin. MAb ATB recognizes free aflatoxins B1 and B2. No cross-reactivity with G1, G2 and M1 was observed.
Ordering information: Product
Cat.#
MAb
Isotype
Remarks
Anti-Aflatoxin
3Af27
ATB
IgG1
EIA
58
INFECTIOUS DISEASE REAGENTS