Posterpreis – Jury WissenschaftsjournalistInnen
2. Platz: Dr. Sabina Essler (Einreichende, Institut für Immunologie), Mag. Werner Ertl, Dr. Julia Deutsch, Dr. Barbara Rütgen, Sandra Groiss, Maria Stadler, Bhuma Wysoudil, MSc, DI Dr. Wilhelm Gerner, Chak-Sum Ho, PhD und Univ.Prof. Dr. Armin Saalmüller: Molecular characterization of swine leukocyte antigen gene diversity in purebred pietrain pigs
1. Platz, ex aequo: Mag. Nora Mareike Biermann (Einreichende, Klinische Abteilung für Pferdechirurgie), Mag. Nora Rindler, Ao.Univ.Prof. Dr. Heinz Buchner: The effect of pulsed electromagnetic fields on back pain in polo ponies evaluated by pressure algometry and flexion testing
3. Platz:
Mag. Nora Rindler (Einreichende, Klinische Abteilung für Pferdechirurgie), Mag. Nora Mareike Biermann, Dr. Simone Westermann und Ao.Univ.Prof. Dr. Heinz Buchner: The effect of pulsed electromagnetic fields on surface temperature at the back of sporthorses – a thermographic study
Dr. Gabrielle Stalder, Dr. Igor Loncaric (Einreichende, Forschungsinstitut für Wildtierkunde und Ökologie bzw. Institut für Bakteriologie, Mykologie und Hygiene) und Univ. Prof. Dr. Chris Walzer: They slime, they ooze, they zoonooze – characterization of enterobacteriaceae with zoonotic potential isolated from the faeces of the spanish slug (Arion lusitanicus)
Molecular characteri zation of swine leukocyte antigen gene diversity in purebred Pietrain pigs
contact: sabine.essler@vetme duni.ac.at
S.E. Essler1, W. Ertl1 , J. Deutsc 1 C.-S. Ho3 and A. Saalmü 1 h , B.C. Rütgen2, S. Groiss1, M. Stadler1 , B. Wysou ller
dil1
, W. Gerner1, of Immunology and 2 Clinical Pathology 3Gift Platform, Departme of Life Michigan, Histocomp nt of Pathobiology, atibility Lab, Ann Arbor, University of Veterinary Michigan, USA Medicine Vienna, Austria 1Institute
SLA class II
Genomic organizatio n of antigen (SLA) complex the swine leukocyte class I and class region. II
SLA class I
Background >> The swine leukocyte antigen harbours the SLA (SLA) complex, class I and II gene
clusters. These genes highly polymorphic are and encode cell responsible for antigen surface glycoproteins presentation with high the adaptive immune response against vaccine importance e.g. in antigens.
Collect Blood
Extract DNA 30 min
Work flow for the PCR-SS
Prepare PCR 30 min
Run PCR 60 min
Run Gel 30 min
P > The assessme genotype can be accomplis nt hed in less than 3 hours of the individual SLA after blood sampling .
SLA class I
E1: SLA1*04XX
SLA class II
G5: SLA2*04XX A3: SLA3*04XX
Modified after Lunney et al., Dev. Comp. 33:362-74. Note: Not Immunol. 2009 all the genes are shown here and the scale is approximate.
Objectives >>
In Austria, the majority of commercial pigs descendants of F1 are F2 Large White/Landrace hybrids paired with Pietrain boars. The repertoire of SLA alleles and haplotypes present in Pietrain pigs thus has an important influence on that descendants. In this of their study, we SLA-gen otyped 27 purebred pigs with a low-reso Pietrain lution PCR-based method using allele-gr sequence-specific oup primers (PCR-SSP). SLA Class II SLA allele specificity haplotype DRB1 DQB1 DQA 01XX
Lr-0.01 Lr-0.06 Lr-0.12 Lr-0.13 Lr-0.14 Lr-0.15a Lr-0.19a Lr-0.20 Lr-0.23 Lr-0.24 Lr-0.25 Lr-Pie-0.2 Lr-Pie-0.1
01XX 01XX 05XX 08XX 01XX 06XX 07XX 01XX 04XX 03XX 02XX/ka01 09XX/La02 08XX 03XX 04XX 02XX 02XX 04XX 07XX 03XX 06XX 03XX 01XX 10XX/er01 06XX/zs12 01XX 07XX 02XX/zs13 02XX 13XX 09XX 04XX+05XX 06XX 03XX 03XX 01XX/be01/ha04 05XX blank
E7: DRB1*02XX D10: DQB1*04XX F12: DQA*02XX
C4: SLA3*04XX
haplotype frequency 5.56% 1.85% 7.41% 7.41% 25.93% 5.56% 16.67% 3.70% 9.26% 1.85% 11.11% 1.85% 1.85%
SLA Class I SLA allele specificity haplotype SLA-1 SLA-3 SLA-2
Lr-01.0 Lr-11.0 Lr-24.0 Lr-25.0 Lr-26.0 Lr-28.0 Lr-29.0 Lr-32.0 Lr-35.0 Lr-39.0 Lr-40.0 Lr-43.0 Lr-55.0 Lr-64.0 Lr-67.0
Lr-4.0 Lr-0.4
Application of PCR-SS P
> This homozygous PCR-based approach pig has been typed for the low-resolution by this and Lr-0.4 (SLA class haplotypes Lr-4.0 (SLA class I) II).
SLA-typing results for
01XX 01XX 01XX 09XX 07XX 05XX blank 04XX/hb06 06XX 11XX 03XX 07XX 08XX 05XX 10XX 09XX, 15XX 07XX 05XX blank 05XX w09XX 07XX 04XX/hb06 02XX 12XX, 13XX 05XX 10XX blank 05XX 10XX 16XX 05XX 10XX 11XX 04XX/hb06 04XX 15XX 04XX/hb06 es22 14XX 05XX 10XX 15XX 05XX 10XX
haplotype frequency 5.56% 1.85% 16.67% 7.41% 7.41% 3.70% 1.85% 1.85% 7.41% 1.85% 1.85% 20.37% 11.11% 5.56% 1.85%
haplotypes were identified 27 Pietrain pigs > 15 class I and 13 class II in the studied cohort. never been reported 2 class II haplotype in other pig populatio s ns, suggesting a breed-spehave constriction of SLA gene diversity in Pietrain cific pig.
Outloo
k >> PCR-based SLA-hap lotyping will facilitate of SLA influence the study in various immuno logical conditions contribute to the improve and may ment of swine health. DQB1 08XX
DQA 03XX
DRB1 09XX
Centromere
SLA-2 SLA-3 SLA-1 04XX
04XX
Innate
11XX Immune
0.14
Viral, bacterial and parasitic system
Adaptive
Definition of SLA
infections
Identification of T-cell epitopes
43.0
Lr-43.14
haplotypes >
The most frequent Lr-43.14 (18.5%; overlap haplotype associatio of 20.37% for Lr-43.0 n has been found and 25.93% for Lr-0.14) among that demonstrate the definition the studied Pietrain pigs is shown here to of SLA haplotype typing assay. s in swine by the PCR-based
MHC = SLA - swine leucocyte antigens
SLA-typed pigs in Austria/EU
improved breeding strategies prophylaxis
improved and novel vaccines prophylaxis
Consumer Protection Consumer Acceptance
Acknowledgements >> For the collaborati on by providing samples work is part of a start-up for project (“Profillinie 2”) funded by the University this study we are indebted to DI Christian Draxl of Veterinary Medicine Vienna, Austria. Reference (“Österreichische Schweineprüfanstalt”, Streitdorf, Austria). : Essler SE et al. Anim This Genet. 2012 May 16. PMID: 22587706.
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