Earthwatch_Book by Vânia Lopes

Seed Bank of two species in danger of extinction

“Peucedanum schottii & Silene sennenii”

Earthwatch Project

Project title:

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project INDEX Project Overview The Botanic Garden Project Staff General Description of the project Total Budget Research Species: Silene sennenii Research Species: Peucedanum schottii Bess. ex DC. (Apiaceae) Decription of a Seed Bank Field Force_Silene sennenii Field Force_Peucedanum schottii Germination testing of the protected species in Catalunya

APPENDIX DECREE LAW (Catalan language) RECORDS DATABASE (Catalan language) PRESS RELEASE NEWSPAPER

Project title:

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project Project Overview After very intensive research with Vânia Lopes (Landscape Architect), the selection was the “Fundació Carl Faust”. This Institution have been managed The Botanic Garden Marimurtra from Blanes, Girona. This Garden is the most interesting botanic garden from the landscape point of view. This non-governmental organization has been focusing the work on biology, conservation and communication. Due to the fact, nowadays, we are observing an increase in the numbers of species in danger of extinction, the contribution of the botanic gardens is very important for the conservation of the global biodiversity. The Botanic Garden Marimurtra is the first garden to have a “Germoplasma” security system in Catalunya, this means they have more than one freezen to prevent possible issues. (www.marimurtra.cat). They organized “Les primers jornadas catalanes de conservació de flora Catalana” on 3rd and 4th of June 2008. They comented about the “Cataleg de Flora amenaçada de Catalunya” that was drafted by the “Govern de la Generalitat”. In this booklet, 59 species in danger of extinction and 123 “vulnerable” species have been included. In the last legislation there only had 5 protected species. This year, and because this project is expensive and they have a limited grant from “Fundació, Territori i Paisatge, Caixa Catalunya” and “DMAH” (Departament de Medi Ambient i Habitatge), they have collected 25 species. With my project we are going to collect two more species and increase to 27 species in the initial project. And the most interesting thing is that these species should be collected in September, so we could join the scientific group. The person responsible for this collection is Cesar Project title:

Alt Empordá

Blanché, “Catedràtic de Botànica de la Facultat de Farmàcia, UB.” Jordi Masbernat, the Director of Carl Faust Fundation, was very friendly with us and we hit it off in our first meeting, so I am sure that we will do an interesting work. The most important thing is that maybe we can preserve species that are also important to the biodiversity in general and specially in Catalunya, because indirectly they are affected by the changes in the new uses of water and land. “I would like to share with all of you this wonderful project.”

Blanes

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project The Botanic Garden: The “Fundació” and his team have an ambitious Project especially in the conservation of the biology, the fieldwork and the divulgation. At a time when we realize the accelerated process of species in danger of extinction, due to entropic factors, the botanic gardens have an important role to maintain the biodiversity. It is essential to combat climate change and the conservation of the species in danger of extinction. Fully aware of this, the “Fundació Carl Faust” proposes that the Botanic Garden Marimurtra change to a centre for the conservation of species in danger of extinction and for scientific work, especially in the Mediterranean area, seeking to promote civic awareness about the urgency of preserving the green layer of the earth, irreplaceable source of life and health. The implication of the Botanic Gardens of S.XXI the conservation of the diversity: After the II World Congress of Botanic Gardens held in Barcelona in 2004, there is no doubt about the main goal of these institutions, as conservationists of the biology diversity, working in international cooperation (CBCN Newsletter, Vol. 8 number1). The new proposal from the “Comisió Horitzo 2006”, for the JBMiM, established as the strategic orientation, his implication as a garden for the conservation. This new concept of garden is in line with the main cause for the crisis of the biodiversity, i.e., Global Change, as is demanded by the World institutions (United Nations) and Continent Institutions (European Union – EEA-European Environmental Agency). Because of this, instead of the orientation for the conservation, the future activities of the garden will contribute to the study of the consequences about Project title:

the biodiversity and measures of the effects of climate change, in line with the social environment concerns for the future of the planet. This starting point will begin with the design and the role of a modern garden. Some examples of this show us the new trend in the botanic gardens, such as the Climate Change Observations in Botanic Gardens around the Globe Global, http://www.bgci.org/ conservation/news/0323/, Jardin Botanico Canario 2006 (download the Gran Canaria declaration on Climate Change as a PDF).

The Botanic Garden

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project Project Staff: Viviana Lopes, Planning & Reporting Executive from BAT

Alberto del Hoyo, Staff of the Botanic Garden Marimurtra

IBERIA

Bet Figueras, Landscape Architect office from Barcelona.

Vania Lopes, Director of OPERA Landscape of idOPERA,

Rosa Merlo, Staff of the Botanic Garden Marimurtra

Mª Carmen Martinell Andreu, Staff of the University from

Architecture Office, Lda

Barcelona

Jordi Masbernat, Director of the Botanic Garden Marimurtra

Nuria Membrives, Staff of the Botanic Garden Marimurtra

Cèsar Blanché, Catedràtic de Botànica de la Facultat de Farmàcia, UB

Project title:

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project Description of the project: 1- Study of the best area for the collection, assigned by a scientific of the University from Barcelona……………………………………….………………………......................................….………August

2-Seed Collection…………………….…………………………………................…….…....September

3- Conservation Process (dry, deshidratation and frozen)…………….....................…………starting in September and it will be finished depending on seed characteristics

4- Gene Test to evaluate the potential of the seed………..…..…....................…after seed processing

5- Plant the seed in the JB Marimurtra……………………………....................…….………next spring

6- Analyze the evaluation of these species ………………………......................…during whole project

Total Budget: Description of item/service required

Cost

All the process until frozen step of the “ Silene sennenii”

475€

All the process until frozen step of the “ Peucedanum schottii”

375€

Gene test and plantation

350€

Vania’ s travel costs to Marimurtra

375€

Viviana’ s travel costs to Barcelona and Marimurtra

400€

Final Book of the Project Total cost:

Project title:

0€ 1975€

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project Research Species: Silene sennenii Overview: Endangered Description: Family: Caryophyllaceae Herbs annual or perennial, rarely subshrubs or shrub. Stems and branches usually swollen at nodes. Leaves opposite, decussate, rarely alternate or verticillate , simple , entire , usually connate at base ; stipules scarious , bristly , or often absent. Inflorescence of cymes or cymose panicles, rarely flowers solitary or few in racemes, capitula, pseudoverticillasters, or umbels. Flowers actinomorphic , bisexual , rarely unisexual , occasionally cleistogamous . Sepals (4 or) 5, free, imbricate, or connate into a tube , leaflike or scarious, persistent , sometimes bracteate below calyx. Petals (4 or) 5, rarely absent, free, often comprising claw and limb; limb entire or split, usually with coronal scales at juncture of claw and limb. Stamens (2--) 5--10, in 1 or 2 series. Pistil 1; carpels 2--5, united into a compound ovary. Ovary superior, 1-loculed or basally imperfectly 2--5-loculed. Gynophore present or absent. Placentation free, central, rarely basal; ovules (1 or) few or numerous, campylotropous. Styles (1 or) 2--5, sometimes united at base. Fruit usually a capsule, with pericarp crustaceous, scarious, or papery, dehiscing by teeth or valves 1 or 2 Ã? as many as styles, rarely berrylike with irregular dehiscence or an achene. Seeds 1 to numerous, reniform , ovoid , or rarely dorsiventrally compressed , abaxially grooved , blunt , or sharply pointed , rarely fimbriate-pectinate; testa granular , striate or tuberculate , rarely smooth or spongy ; embryo strongly curved and surrounding perisperm or straight but eccentric ; perisperm mealy. Between 75 and 80 genera and ca. 2000 species: Project title:

Flower during its first night of anthesis (masculine period)

widespread but mainly of temperate or warmtemperate occurrence in the N hemisphere, with principal centers of distribution in the Mediterranean region and W Asia to W China and the Himalayas, fewer species in Africa S of the Sahara, America, and Oceania; 30 genera (two endemic) and 390 species (193 endemic) in China. Arenaria, Silene, and Stellaria contain over half the species in the family in China. They are mostly concentrated in the Qinghai-Xizang plateau, and are especially rich from the Hengduan Mountains to the Himalayas. The main uses of this family are medicinal and ornamental. Dianthus superbus, Pseudostellaria heterophylla, Stellaria dichotoma var. lanceolata, and Vaccaria hispanica are commonly used in traditional Chinese medicine. Some species of Arenaria, Dianthus, Gypsophila, Psammosilene, and Silene are used as medicinal herbs among the people or are habitually used in local Chinese medicine. Many species of Dianthus, Gypsophila, Lychnis, Saponaria, and Silene are grown as ornamentals. Atocion armeria

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project (Linnaeus) Rafinesque ( Silene armeria Linnaeus), native to Russia and Europe, is also cultivated in China. It differs from Silene in having a corymbose inflorescence and obscure calyx veins. Wu Cheng-yih, Ke Ping, Zhou Li-hua, Tang Chang-lin & Lu De-quan. 1996. Caryophyllaceae. In: Tang Chang-lin, ed., Fl. Reipubl. Popularis Sin. 26: 47â??449.1 Genus Silene Herbs, annual, biennial, or perennial, often decumbent at base or sometimes cespitose. Taproots slender or often stout, deep, branched caudex often present, some species stoloniferous or rhizomatous. Stems simple or branched, terete or sometimes angular. Leaves opposite or occasionally whorled, connate proximally, petiolate (basal leaves) or sessile (most cauline leaves); blade 1-5-veined, linear to obovate or spatulate, herbaceous, apex acute to obtuse. Inflorescences terminal or sometimes axillary, simple or branched, sometimes condensed cymes, frequently flowers few or solitary, frequently glandular-pubescent and viscid; bracts paired, herbaceous or scarious, or absent; involucel bracteoles absent. Pedicels erect, rarely flowers sessile or subsessile. Flowers bisexual , sometimes unisexual (rarely so on separate plants ) ; sepals connate proximally into tube , (4-) 1028(-40) mm; tube green, whitish, and/or purplish, 10-30-veined, cylindric to campanulate , urceolate , or clavate , terete, frequently inflated , membranous or more rarely herbaceous, commissures between sepals 1-veined, herbaceous; lobes green or purplish, 1-5-veined, broadly triangular to lance-oblong or linear, usually shorter than tube, margins whitish, scarious, apex acute to obtuse; petals 5, white, pink, scarlet, dusky purple, or off-white tinged with purple, clawed, claw usually conspicuous , sometimes small, rarely absent, auricles 2, coronal appendages 2, variously shaped or dissected ; limb usually exserted and Project title:

During its last night (femenine period)

conspicuous, oblanceolate to obovate, apex 2-lobed, sometimes dissected into 1-4 linear lobes or irregular teeth, or fimbriate, rarely entire ; nectaries at filament bases; stamens 10, rarely fewer or absent, frequently dimorphic with longer opposite petals, arising with petals from carpophore; filaments distinct nearly to base; staminodes absent (rarely to 10 in pistillate flowers, arising with petals from carpophore, filiform ) ; ovary 1- or 3-5-locular; styles 3 or 5, occasionally 4 (absent in staminate flowers ), filiform, 1.5-20 mm, glabrous proximally; stigmas 3 or 5, occasionally 4, linear along adaxial surface of styles, papillate (30×) . Capsules ovoid to globose , opening along sutures into 3-5 valves , frequently splitting into 6-10 equal teeth; carpophore usually present. Seeds ca. (5-) 15-100(-

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project 500+), reddish to gray or black, reniform to globose, usually tuberculate or papillate, papillae around margins sometimes larger and inflated, marginal wing sometimes present, appendage absent; embryo peripheral, curved . x = (10) 12. Species ca. 700: mainly Northern Hemisphere. Silene includes several important weeds and some very beautiful horticultural plants. In addition to the species described in this account, several others have occurred in the flora area as chance introductions or garden escapes, but they have not become established and most have not been seen recently. They include S. coeli-rosa (Linnaeus) Godron, S. cretica Linnaeus, S. (Lychnis) fulgens (Fischer) E. H. L. Krause, S. italica Persoon, and S. nutans Linnaeus. In this account, Lychnis, Melandrium, and Viscaria have been included in Silene, their previous recognition as distinct genera having resulted in a great deal of confusion in both nomenclature and taxonomy. I have not presented an infrageneric classification of Silene because existing systems either do not include those other genera (e.g., P. K. Chowdhuri 1957) or do not deal with most of our native North American taxa [e.g., W. Greuter (1995) and the molecular studies by Oxelman and coworkers (e.g., B . Oxelman et al. 1997, 2000). The recent molecular study by J. G. Burleigh and T. P. Holtsford (2003) provides little

support for existing morphologically based sectional classifications within Silene insofar as they relate to endemic North American taxa. However, it does indicate the distinctness of our arctic alpine species (S. involucrata??as S. furcata, and S. acaulis) that are circumpolar in their distribution.2

Physical Description Habit: Clumping perennial. evergreen. Flowers: Single pink or pale pink flowers in April, May, June, July. Attracts butterflies and hummingbirds. Petals 5, clawed. Limb crimson, 2cm long, 6mm broad, notched at apex, glabrous. Claw -2cm long, mostly scarious but reddish near apex, glabrous. Fornices 2, 3-4mm long, erect, red. Stamens 10, half adnate at base of petals, half not adnate to petals, exserted. Filaments 2.4cm long, glabrous, greenishwhite below, reddish near apex. Anthers 3mm long, 2-lobed, greyish-green. Ovary on small gynophore(to 1.5mm long), cylindric , yellow-green, 6mm long, -2mm in diameter. Placentation free-central. Ovules many. Styles 3, white below, red above, -2cm long. Calyx tube to 1.7cm long, 5-lobed, densely glandular pubescent , often with a reddish tinge, 10-nerved, glabrous internally. Lobes acute, triangular, 4mm long. Foliage: Narrow, strap-like lanceolate leaves. Stems and the bases of flowers are covered with sticky hairs.

During its last night (femenine period) Project title:

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project Leaves of basal rosette spatulate , petiolate , to +/15cm long (with petiole ), 2cm broad, acute, entire . Blades mostly glabrous . Margins ciliate , especially on petiole. Cauline leaves opposite, becoming sessile, lanceolate to lance-linear, entire, viscid glandular pubescent , acute, reduced upward, typically less than 8 pairs on a stem.

Size/Age/Growth Size: 12-18” tall.

Landscaping Landscape Uses: Rock gardens. Wildflower gardens. Shade gardens. • Care: Tolerates coastal conditions.

Habitat Ecology: This perennial herb generally grows in primary or secondary habitats dominated by Brachypodietum phoenicoidis. (Ref. 83280)

Biology

Growth: Culture: Space 15-18” apart. Soil: Moderately fertile , well-drained soil. Sunlight: Sun Exposure: Sun to partial shade. Likes sun. Moisture: Water Requirements: Drought tolerant . Temperature: Cold Hardiness: 3a, 3b, 4a, 4b, 5a, 5b, 6a, 6b, 7a, 7b, 8a, 8b, 9a, 9b, 10a, 10b, 11. (map)

During its last night (femenine period) Project title:

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project Research Species: Peucedanum schottii Bess. ex DC. Overview: Critically endangered Description: Family Apiaceae Herbs, annual or perennial, rarely woody at base. Caulescent or acaulescent , stem hollow or solid. Leaves alternate, rarely opposite or basal; petiole usually sheathing at base; stipules absent (except in subfam. Hydrocotyloideae) ; leaf blade compound or sometimes simple , usually much incised or divided , pinnatifid to pinnatisect , or ternate-pinnately decompound . Flowers epigynous , small, bisexual or staminate (unisexual male), regular, in simple or compound umbels; umbellules few to manyflowered; rays often subtended by bracts forming a involucre; umbellules (sometimes called umbellets ) usually subtended by bracteoles forming an involucel . Pedicels long, short or obsolete (then forming a capitate umbellule). Calyx tube wholly adnate to the ovary; calyx teeth (sometimes called sepals) small or obsolete, forming a ring around the top of the ovary. Ovary inferior, 2-celled, with one anatropous ovule in each locule. Styles 2, usually swollen at the base forming a stylopodium which often secretes nectar. Fruit dry, of two mericarps united by their faces (commissure ), and usually attached to a central axis (carpophore), from which the mericarps separate at maturity; mericarps are variously flattened dorsally , laterally or terete ; each mericarp has 5 primary ribs , one down the back (dorsal rib), two on the edges near the commissure (lateral ribs), and two between the dorsal and lateral ribs (intermediate ribs), occasionally with four secondary ribs alternating with the primary, the ribs filiform to broadly winged , thin or corky; Project title:

Peucedanum schottii

vittae (oil-tubes) usually present in the furrow (intervals between the ribs sometimes called the valleculae) and on the commissure face, rarely also in the pericarp, sometimes obscure . Each mericarp 1-seeded, splitting apart at maturity. Seed face (commissural albumen) plane, concave to sulcate . Between 250 and 440( 455) genera and 3300 3700 species: widely distributed in the temperate zone of both hemispheres, mainly in Eurasia and especially in C Asia; 100 genera (ten endemic) and 614 species (340 endemic) in China. Genus Peucedanum Herbs perennial. Rootstock short, crown usually bearing remnant leaf sheaths. Stem finely striate, dichotomously branched above. Leaves petiolate; petioles sheathing. Umbels loosely compound, terminal and lateral; bracts numerous or absent; rays numerous or few; bracteoles numerous, rarely few or absent. Calyx teeth short or obsolete. Petals usually white, occasionally pinkish or purplish, rarely pale yellow, orbicular to obovate, with a narrow inflexed

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project

Peucedanum schottii

apex. Stylopodium low-conic. Fruit ellipsoid , oblong to suborbicular , dorsally compressed ; dorsal ribs filiform , slightly prominent , lateral ribs thickened and narrowly winged , wings closely appressed to one another; vittae 1 to several in each furrow, 2 to several on commissure . Seed face plane or slightly concave. Carpophore bipartite. Between 100 and 200 species: Africa, Asia, Europe; 40 species (33 endemic) in China. The taxonomy of this widespread, heterogeneous genus has long been problematic. The broad circumscription of Peucedanum adopted here includes some 100120 species from many parts of the Old World that are weakly united by basic (especially external) fruit structure. These fruit characters are undoubtedly the result of several lines of convergent evolution, and some authors prefer to recognize several segregate genera and reduce Peucedanum to only 810 Eurasian species based around the nomenclatural type, P. officinale Linnaeus. Project title:

Hábitat

Ecology: Meadows of Festuca gautieri, in high and shady locations, approximately at 1100m.

Population Fluctuating number of copies reproducing over the years. Part of the population does not reproduce due to the intense grazing in the area. Data base of 2007: 97 ind. widgets y 157 ind. with stalks eaten by livestock. Occupation taxon: 0.4 km2. Unique population in the Iberia region.

Diagnosis Plant very rare and not very abundant in the locality, and difficult to find seeing as it can be confused with other species of the same family (Seseli montanum) present in the area. We must carry out prospecting in the fruit-bearing period, because it is easier to differentiate from similar taxa. With major fluctuations in the number of breeders, fruit bearing strems, and suffers an intense predation (sheep and goats).

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project Period prospecting

Political Regions

Second half of September and first half of October.

Parameters to follow Number of copies players, number of non-breeding copies, number of stem, number of copies with impact (eaten or stunted), area of occupancy and rate by coating sectors.

Observations All indicators that have been registered, although the record is not very long, show the worrying situation of the town: • few breeders, with significant fluctuations and with a tendency to decrease. • a low proportion of branches that produce fruitful results, a high percentage of stunted stalks and especially eate

Impacts and current threats Grazing (sheep and goats) with intense predation on the plant, dumping of construction debris, trampling and habitat degradation. In addition, vehicle traffic and possible reforms of nearby buildings.

Recommendations Do not alter the pastures where it grows. Increase their habitat through clearing rubble and restoring degraded areas where there are still some exemples. These actions could increase the number of individuals. Priority Action: dramatically reduce the pressure of livestock (elimination of goats and installation of an electrified fence to prevent access of sheep throughout in all potential areas at the time of development of the plant). Regulation of public and vehicle transit in certain areas. Surveys in similar environments in the area.

Figueres

Mare de Deu del Mont, Segueró, Alt Empordá Project title:

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project General description of a Seed Bank: It is a collection of seeds conserved in special conditions which ensure the viability for a long period of time. 1- Preview selection of the species to be conserved 2- A review of the bibliography information 3- Plan of the expedition for the recollection 4- Recollection of the fruits and seeds 5- Registration in the database This includes: Taxonomic information (family, genre, specie and author) Recollection date Local of recollection (town, UTM and high) Information of the recollection 6- Creation of the sheet (testimony of the recollection) 7- Cleaning of the seeds 8- Dehydration (humidity of 3-7%) 9- Airtight bottle 10- Conservation of the seeds: • Medium and long term (> 3 years) – fridge at – 18 degrees • Short term (< 3 years) – freeze at 4 degrees 11- Control of the viability: germination test • Before conservation • Every 5 – 10 years to ensure the viability with time

Seeds

Sheet

Bags

Airtight bottle

Conservation Sample conservation

But…: The seed bank must never be an excuse for substituting the natural population. It should act as a reserve to ensure the future of this population in CASE OF NECESSITY.

Sample conservation Project title:

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project Field Force_Silene sennenii 1St Population - Castell de Sant Ferran, Figueres

2nd Population - Riu Manol, Figueres

3th Population - Far, Figueres

4th Population - Baseia, Figueres

Project title:

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project Field Force_Peucedanum schottii 1St Population - Mare de Deu del Mont, Segueró

Project title:

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project Lab’s Inauguration_19th September 2008

Project title:

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project Process of.... Removing dirt, dehydration and weighing to estimate number of the seeds in sample. The seeds required for testing are separated and the rest are stored in airtight tubes at -18ºC.

Methodology

Project title:

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project Germination testing of the protected species in Catalunya Peucedanum schottii & Silene sennenii For the germination test we used plate of “Petri” with “agar” farming at 1% The testing was carried out with 25 seeds of Peucedanum schottii and 100 seeds for Silene sennenii. Methodology 1) Disinfection with NaOC1 at 1% for 2 minutes (date: 17/04/2009) 2) Soek in distilled water (minimum 48 hours) (date: 17/04/2009) 3) Preparation in plate for part of the sample (date: 20/04/2009 a. Peucedanum schottii (10 seeds): direct seedtime in plate b. Silene sennenii (50 seeds): direct seedtime in plate 4) Pre-treatment with “giberalinas” (date: 20/04/2009) 5) Preparation in plate of the rest of the sample (date: 21/04/2009) a. Peucedanum schottii (15 seeds): direct seedtime in plate b. Silene sennenii (50 seeds): direct seedtime in plat

Methodology

The samples are introduced in the seed bank in conditions of 12 hours with light of 20ºC and 12 hours in the dark at 15ºC.

Project title:

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project Process of preparing for the seedtime in a seed bank in the middle of “agar” farming (1%). Realization of the pretreatment selected.

Methodology

Project title:

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project Germination_Silene sennenii Germination in the agar plate, seed transfer and transplantation in pots in the investigation nursery garden from the Botanic Garden Marimurtra.

Firts sign of germination

Firts germination

1st Plantation_Silene sennenii Peaked

Germination date: April 2009 Plantation date: June 2009 Plant status: leaves forming a basal rose Nº of plants used: 10 Substratum used: “Sauló” Year to date results: the 10 plants survive but they didn’t thrive with the same intensity as the same ones reserved in the nursery garden and it is possible that some may end up dying.

Plantation process

Developing seedling

Greenhouse

Potted plant

Project title:

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

1st Plantaction

Earthwatch Project 2nd Plantation_Silene sennenii

were in flower.

Germination date: April 2009 Plantation date: October 2009 Plant status: samples with flowers Nº of plants used: 10 Substratum used: “Sauló” with terracotem

Observations of the 1st plantation: the species lives in clay soils, consequently less drained. To increase the water retention, we include in the base of the plantation a product that retains the humidity in the

Silene sennenii plantation in the botanic garden collections in the silicícola thicket zone. The transplantation was done in two phases. The first one when the plants were on the “roseta” base and the second when the species

Potted plant

Planting

Flower

Planting Project title:

Team

Planting

Description

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project Actual Status of the Silenni sennenii Despite of the hard winter that we had this year, currently the plants are in very good condition as you can see in the pictures. Summary The project is finished. It is a shame that we couldn’t achieve our total goal, one of the species Peucedanum didn’t survive the germination and couldn’t be planted in the garden together with the “Silenni”. But the most important thing is that we tried and we did all that was up to us. The project was very interesting and gratifying. It was an honour to have the opportunity to work with such a wonderful and committed team. I would like to thank the Botanic Garden of Marimurtra for the kindly reception and the chance to cooperate with their project and also to thank the Earthwatch Institute for the budget needed for this project.

Silenni sennenii Project title:

Silenni sennenii

Silenni sennenii “Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project With Peucedanum we did some more tests but they didn’t work out. Some of them were germinated, but they didn’t survive transplantation. Actually the “umbelíferas” (family of Peucedanum species) are usually too difficult to germinate.

Project title:

Fruit of Peucedanum

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project

Project title:

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project

APPENDIX

Project title:

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project DECREE LAW (Catalan language) 'LDUL 2¿FLDO GH OD *HQHUDOLWDW GH &DWDOXQ\D 1~P ±

%&1"35".&/5 %& .&%* ".#*&/5 * )"#*5"5(& DECRET 172/2008, de 26 d’agost, de creació del Catàleg de flora amenaçada de Catalunya. /¶DUWLFOH GH O¶(VWDWXW G¶DXWRQRPLD GH &DWDOXQ\D UHJXOD OHV FRPSHWqQFLHV GH OD *HQHUDOLWDW GH &DWDOXQ\D HQ PDWqULD GH 0HGL $PELHQW L HVWDEOHL[ TXH FRUUHVSRQHQ D OD *HQHUDOLWDW GH &DWDOXQ\D OD FRPSHWqQFLD FRPSDUWLGD HQ PDWqULD GH PHGL DPELHQW L OD FRPSHWqQFLD SHU D O¶HVWDEOLPHQW GH QRUPHV DGGLFLRQDOV GH SURWHFFLy /D FRPSHWqQFLD FRPSDUWLGD LQFORX HQWUH G¶DOWUHV OD UHJXODFLy GHOV UHFXUVRV QDWXUDOV GH OD ÀRUD L GH OD IDXQD L GH OHV PHVXUHV GH SURWHFFLy GH OHV VHYHV HVSqFLHV HQ HO PDUF GH OHV EDVHV TXH ¿[L O¶(VWDW FRP D SULQFLSLV R PtQLP FRP~ QRUPDWLX /D 'LUHFWLYD &(( GHO &RQVHOO GH GH PDLJ GH UHODWLYD D OD FRQVHUYDFLy GHOV KjELWDWV QDWXUDOV L GH OD IDXQD L ÀRUD VLOYHVWUH HVWDEOHL[ OHV HVSqFLHV SURWHJLGHV L DPE SUREOHPHV GH FRQVHUYDFLy D (XURSD (O 5HLDO GHFUHW GH GH GHVHPEUH PRGL¿FDW SHO 5HLDO GHFUHW GH GH MXQ\ SHO TXDO V¶HVWDEOHL[HQ PHVXUHV SHU D FRQWULEXLU D JDUDQWLU OD ELRGLYHUVLWDW PLWMDQoDQW OD FRQVHUYDFLy GHOV KjELWDWV QDWXUDOV L GH OD IDXQD L ÀRUD VLOYHVWUH pV HO PDUF QRUPDWLX EjVLF YLJHQW /D /OHL HVWDWDO GH GH GHVHPEUH GHO SDWULPRQL QDWXUDO L GH OD ELRGLYHUVLWDW FRQVWLWXHL[ HO PDUF MXUtGLF EjVLF SHU D OD FRQVHUYDFLy ~V VRVWHQLEOH L PLOORUD GHO SDWULPRQL QDWXUDO L GH OD ELRGLYHUVLWDW /¶DUWLFOH G¶DTXHVWD /OHL HVWDEOHL[ TXH OHV FRPXQLWDWV DXWzQRPHV DGRSWDUDQ OHV PHVXUHV QHFHVVjULHV SHU JDUDQWLU OD FRQVHUYDFLy GH OD ELRGLYHUVLWDW TXH YLX HQ HVWDW VLOYHVWUH DWHQHQW SUHIHUHQWPHQW D OD SUHVHUYDFLy GHOV VHXV KjELWDWV L KD G¶HVWDEOLU UqJLPV HVSHFt¿FV GH SURWHFFLy SHU D DTXHOOHV HVSqFLHV VLOYHVWUHV OD VLWXDFLy GH OHV TXDOV KR UHTXHUHL[L PLWMDQoDQW OD VHYD LQFOXVLy HQ DOJXQD GH OHV FDWHJRULHV HVWDEOHUWHV D O¶DUWLFOH GH OD /OHL /¶HVPHQWDW SUHFHSWH HVWDEOHL[ HO &DWjOHJ HVSDQ\RO G¶HVSqFLHV DPHQDoDGHV L UHJXOD OHV FDWHJRULHV HQ TXq KDQ GH VHU FODVVL¿FDWV HOV Wj[RQV R SREODFLRQV GH OD ELRGLYHUVLWDW DPHQDoDGD G¶DFRUG DPE OD LQIRUPDFLy WqFQLFD R FLHQWt¿FD TXH DL[t KR DFRQVHOOL L GLVSRVD TXH OHV FRPXQLWDWV DXWzQRPHV SRGUDQ HVWDEOLU FDWjOHJV G¶HVSqFLHV DPHQDoDGHV HQ TXq HVWDEOHL[LQ D PpV GH OHV FDWHJRULHV UHODFLRQDGHV HQ DTXHVW DUWLFOH DOWUHV G¶HVSHFt¿TXHV L GHWHUPLQLQ OHV SURKLELFLRQV L DFWXDFLRQV QHFHVVjULHV SHU D OD VHYD SUHVHUYDFLy /¶$WODV GH OD )ORUD 9DVFXODU $PHQDoDGD L OD /OLVWD 9HUPHOOD GH OD )ORUD 9DVFXODU HVWDWDOV DL[t FRP HOV LQIRUPHV GHOV 3DUFV 1DWXUDOV GH &DWDOXQ\D KDQ SHUPqV FRQqL[HU OHV HVSqFLHV TXH DFWXDOPHQW UHTXHUHL[HQ PHVXUHV XUJHQWV GH FRQVHUYDFLy L HV FRQVWDWD TXH GLYHUVHV G¶DTXHVWHV HVSqFLHV VyQ D SXQW G¶H[WLQJLU VH SHU OD TXDO FRVD UHVXOWD LPSUHVFLQGLEOH UHJXODU OHV L DGDSWDU OHV D OHV FDWHJRULHV H[LVWHQWV D OD QRUPDWLYD EjVLFD HVWDWDO &RP D FRQVHT qQFLD G¶DL[z L DWHVD O¶H[LVWqQFLD G¶XQD VqULH G¶HVSqFLHV L VXEVSqFLHV GH OD ÀRUD TXH HV WUREHQ DPHQDoDGHV DO WHUULWRUL GH &DWDOXQ\D L TXH UHTXHUHL[HQ PHVXUHV GH FRQVHUYDFLy FDO DGRSWDU OHV PHVXUHV QHFHVVjULHV SHU WDO GH SURWHJLU DTXHVWHV HVSqFLHV L VXEVSqFLHV G¶DFRUG DPE HOV YDORUV HFROzJLFV L DPELHQWDOV TXH SRVVHHL[HQ $PE DTXHVWD ¿QDOLWDW DTXHVW 'HFUHW FUHD HO &DWjOHJ GH ÀRUD DPHQDoDGD GH &DWDOXQ\D L FDWDORJD DTXHOOHV HVSqFLHV L VXEVSqFLHV DPHQDoDGHV TXH HV FODVVL¿TXHQ HQ OHV FDWHJRULHV GH ³HQ SHULOO G¶H[WLQFLy´ R GH ³YXOQHUDEOHV´ HQ IXQFLy GHO VHX HVWDWXV GH FRQVHUYDFLy L JUDX G¶DPHQDoD L HVWDEOHL[ OHV PHVXUHV GH FRQVHUYDFLy GH OHV HVSqFLHV L VXEVSqFLHV FDWDORJDGHV L HO UqJLP G¶H[FHSFLRQV GH OHV SURKLELFLRQV UHJXODGHV DO 'HFUHW $ PpV V¶HVWDEOHL[ HO SURFHGLPHQW GH FDWDORJDFLy GHVFDWDORJDFLy L GH FDQYL GH FDWHJRULD GH OHV HVSqFLHV L VXEVSqFLHV )LQDOPHQW V¶HVWDEOHL[ HO UqJLP VDQFLRQDGRU DSOLFDEOH D OHV LQIUDFFLRQV L OD YDORUDFLy HFRQzPLFD GH OHV HVSqFLHV L VXEVSqFLHV REMHFWH GH UHJXODFLy

%JTQPTJDJPOT

Project title:

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project RECORDS DATABASE (Catalan language) Far FITXA RECOL·LECCIÓ GERMOPLASMA N.

ID progressiu:

Data ingrès:

Taxon: Silene sennenii

Operador:

Flora utilitzada per la determinació:

Dades poblacionals: Codi de població: Estat: Espanya Regió: Catalunya Província: Girona Comarca: Alt Empordà Localitat: Far de l'Empordà UTM: 4678100 499700 N ° ' '' E ° ' '' Instrument utilitzat: Precissió de les dades: Alçada: mitjana ( min max ) Inclinació: mitjana ( min max ) Exposició: mitjana ( de a Litologia: Superfície roques ( % ) : Superfície pedres ( % ) : Classif. pedològica:

Nom del recol·lector/codi: Entitat a la que pertany el recol·lector/codi: Projecte: Propietat del material: Finalitat de la recol·lecció: !X! Banc de germoplasma 100 % !__! Llavors actives _____ %

Horitzonts: Drenatge: 1 2 3 4 5 Bioclima: Termotipus: Ombrotipus: Tipus de vegetació:

!__! Altres: _____ % Especificar _________________________ Data i hora de recol·lecció: Temp. (T°C):

21/08/08 Tarda Humitat Relativa (%): P. atmosfèrica (hPa-mbar)

~25ºC ~60% Condicions meteorològiques:

!__! Camp. pH: _____ Coerenza: 1 2 3 4 5

Codi Corine:

Codi Hàbitat:

Sol amb núvols i bastant vent Mètode de mostreig:

Àrea mostrejada (m2): 7000 Àrea població (m2): 7000

!X! Regularment distribuït per tota la superfície

Macropoblació !__!

!__! Al centre de la població

N. Micropoblacions !__!

!__! Al llarg d'una línia transversal

Estructura de la població: !X! Individus aillats (1) !__! Petits grups (2) !__! Grups (3) !__! Colònies (4) !__! Població (5)

!__! Al llarg d'una línia marginal !__! Altres:

N. individus presents: 1-5!_! 10000!_! >10000!_!

6-10!_!

11- 50!_!

51-100!X!

101-250!_!

251-500!_!

)

Dimensions (m2): Fitxa Demogràfica: Si

501-1000!_!

Ref. cartográfica: SI No N. de lots d'aquesta recol·lecció:

1001-2500!_!

2501-5000!_!

5001-

Material recol·lectat:

N. de llavors/fruit:

!X! Llavors

!__! Bulbils

N. de llavors/individu:

% plantes joves:

!__! 000 sense flors

!__! Fruits

!__! Bulbs

N. de llavors col·lectades/individuo:

% plantes adultes:

!__! +00 només flors tancades

N. individu sobre els quals s'ha fet la recol·lecta:

% plantes mortes:

!__! ++0 flors tancades i obertes

Causa:

!__! +++ flors tancades, obertes i marcents

!__! Pol·len

!__! Rizomes

!__! Espores

!__! Tubercles

!__! Esqueixos !__! Altres: Estat de la llavor:

Estat fenològic predominant

% plàntules:

!__! 0++ flors obertes i marcents

% de població productora de llavors: Recollida de: Planta !X!

Humit !__!

Sec !X!

N. de llavors proba del tall:

Sòl !__!

Inflorescència o flor amb:

Altres: % èxit positiu:

Dades morfomètriques:

!__! 00+ només flors marcents

Floració simultània !__!

!__! 00+b con fiori appassiti e bacche acerbe

Floració escalonada !__!

!__! 00+fr flors marcents i fruits immadurs

!__! centrípeta

!__! 000b con bacche mature

!__! centrífuga

!X! 000fr fruits madurs

!__! basal

!X! 000sd semi dispersats !__! altres:

!__! apical Disseminació: Autocora !__! Barocora !X! Anemocora !__! Zoocora !__! Hidrocora !__! Pirocora !__! Igronastica !__! Balistocora !__! Atelecora !__! Policora !__! Altres !__!:

Tipus de material recol·lectat:

Situada en un espai protegit: SI !__! NO !X!

Planta en test:

Tipus:

Mostra d'herbari:

Si !__! No !X! Si !__! No !X!

Fruit test preliminar: Si !__! No !X! Condicions fitosanitàries de la població:

Estat de conservació de la població:

4 4

Mat. biologia molec.: Si !__! No !X!

Mat. cariologia:

Riscs i factors d'amenaça: Agricultura, urbanisme

Si !__! No !X!

Banc de llavors del sòl: Si !__! No !X! Altres: Nota:

Pre-tractaments:

!__! Sense recol·lecta !__! Necessària nova recol·lecta !__! Necessari cultiu per determinació taxonòmica

Pàgina 1

Project title:

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project RECORDS DATABASE (Catalan language) Castell Sant Ferran FITXA RECOL·LECCIÓ GERMOPLASMA N.

ID progressiu:

Data ingrès:

Taxon: Silene sennenii

Operador:

Flora utilitzada per la determinació:

Dades poblacionals: Codi de població: Estat: Espanya Regió: Catalunya Província: Girona Comarca:Alt Empordà Localitat: Castell de Sant Ferran (Figueres) UTM: 4680000 495600 N ° ' '' E ° ' '' Instrument utilitzat: Precissió de les dades: Alçada: mitjana ( min max ) Inclinació: mitjana ( min max ) Exposició: mitjana ( de a Litologia: Superfície roques ( % ) : Superfície pedres ( % ) : Classif. pedològica:

Horitzonts: Drenatge: 1 2 3 4 5 Bioclima: Termotipus: Ombrotipus: Tipus de vegetació:

!__! Altres: _____ % Especificar _________________________ Data i hora de recol·lecció: Temp. (T°C):

21/08/08 Matí Humitat Relativa (%): P. atmosfèrica (hPa-mbar)

~30ºC ~60-80% Condicions meteorològiques:

!__! Camp. pH: _____ Coerenza: 1 2 3 4 5

Codi Corine:

Codi Hàbitat:

Sol i vent Mètode de mostreig:

Àrea mostrejada (m2): 90000 Àrea població (m2): 300000

!X! Regularment distribuït per tota la superfície

Macropoblació !__!

!__! Al centre de la població

N. Micropoblacions !__!

!__! Al llarg d'una línia transversal

Estructura de la població: !__! Individus aillats (1) !X! Petits grups (2) !__! Grups (3) !__! Colònies (4) !__! Població (5)

!__! Al llarg d'una línia marginal !__! Altres:

N. individus presents: 1-5!_! 10000!_! >10000!_!

6-10!_!

11- 50!_!

51-100!_!

101-250!_!

251-500!_!

)

Dimensions (m2): Fitxa Demogràfica: Si

501-1000!_!

Ref. cartográfica: SI No N. de lots d'aquesta recol·lecció:

1001-2500!X!

2501-5000!_!

5001-

Material recol·lectat:

N. de llavors/fruit:

% plàntules:

!X! Llavors

!__! Bulbils

N. de llavors/individu:

% plantes joves:

!__! 000 sense flors

!__! Fruits

!__! Bulbs

N. de llavors col·lectades/individuo:

% plantes adultes:

!__! +00 només flors tancades

!__! Pol·len

!__! Rizomes

% plantes mortes:

!__! ++0 flors tancades i obertes

!__! Espores

!__! Tubercles

N. individu sobre els quals s'ha fet la recol·lecta:

Causa:

!__! +++ flors tancades, obertes i marcents

!__! Esqueixos !__! Altres: Estat de la llavor:

Estat fenològic predominant

!__! 0++ flors obertes i marcents

% de població productora de llavors: 90 Recollida de: Planta !X!

Humit !__!

Sec !X!

N. de llavors proba del tall:

Sòl !__!

Inflorescència o flor amb:

Altres: % èxit positiu:

Dades morfomètriques:

!__! 00+b con fiori appassiti e bacche acerbe

Floració escalonada !__!

!__! 00+fr flors marcents i fruits immadurs

!__! centrípeta

!__! 000b con bacche mature

!__! centrífuga

!X! 000fr fruits madurs

!__! basal

!X! 000sd semi dispersats

!__! apical Disseminació: Autocora !__! Barocora !X! Anemocora !__! Zoocora !__! Hidrocora !__! Pirocora !__! Igronastica !__! Balistocora !__! Atelecora !__! Policora !__! Altres !__!: Situada en un espai protegit: SI !__! NO !__!

!__! 00+ només flors marcents

Floració simultània !__!

Tipus:

!__! altres: Tipus de material recol·lectat: Mostra d'herbari: Planta en test:

Si !__! No !X! Si !__! No !X!

Fruit test preliminar: Si !__! No !X! Condicions fitosanitàries de la població:

Estat de conservació de la població:

4 4

Mat. biologia molec.: Si !__! No !X!

Mat. cariologia:

Riscs i factors d'amenaça: Caminants, obres públiques

Si !__! No !X!

Banc de llavors del sòl: Si !__! No !X! Altres: Nota:

Pre-tractaments:

!__! Sense recol·lecta !__! Necessària nova recol·lecta !__! Necessari cultiu per determinació taxonòmica

Pàgina 1

Project title:

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project RECORDS DATABASE (Catalan language) Baseia FITXA RECOL·LECCIÓ GERMOPLASMA N.

ID progressiu:

Data ingrès:

Taxon: Silene sennenii

Operador:

Flora utilitzada per la determinació:

Dades poblacionals: Codi de població: Estat: Espanya Regió: Catalunya Província: Girona Comarca: Alt Empordà Localitat: Baseia (Siurana) UTM: 4673200 501300 N ° ' '' E ° ' '' Instrument utilitzat: Precissió de les dades: Alçada: mitjana ( min max ) Inclinació: mitjana ( min max ) Exposició: mitjana ( de a Litologia: Superfície roques ( % ) : Superfície pedres ( % ) : Classif. pedològica:

Horitzonts: Drenatge: 1 2 3 4 5 Bioclima: Termotipus: Ombrotipus: Tipus de vegetació:

!__! Altres: _____ % Especificar _________________________ Data i hora de recol·lecció: Temp. (T°C):

21/08/08 Tarda Humitat Relativa (%): P. atmosfèrica (hPa-mbar)

~20ºC 60,00% Condicions meteorològiques:

!__! Camp. pH: _____ Coerenza: 1 2 3 4 5

Codi Corine:

Codi Hàbitat:

Sol i núvols amb vent Mètode de mostreig:

Àrea mostrejada (m2): 8000 Àrea població (m2): 13000

!X! Regularment distribuït per tota la superfície

Macropoblació !__!

!__! Al centre de la població

N. Micropoblacions !__!

!__! Al llarg d'una línia transversal

Estructura de la població: !X! Individus aillats (1) !__! Petits grups (2) !__! Grups (3) !__! Colònies (4) !__! Població (5)

!__! Al llarg d'una línia marginal !__! Altres:

N. individus presents: 1-5!_! 10000!_! >10000!_! Material recol·lectat:

6-10!_!

11- 50!_!

51-100!_!

101-250!_!

N. de llavors/fruit:

251-500!_!

Dimensions (m2): Fitxa Demogràfica: Si

501-1000!_!

Ref. cartográfica: SI No N. de lots d'aquesta recol·lecció:

1001-2500!X!

2501-5000!_!

!__! Bulbils

N. de llavors/individu:

% plantes joves:

!__! 000 sense flors

!__! Fruits

!__! Bulbs

N. de llavors col·lectades/individuo:

% plantes adultes:

!__! +00 només flors tancades

!__! Pol·len

!__! Rizomes

% plantes mortes:

!__! Espores

!__! Tubercles

N. individu sobre els quals s'ha fet la recol·lecta:

Estat de la llavor:

Humit !__!

Sec !X!

N. de llavors proba del tall:

!__! +++ flors tancades, obertes i marcents

Inflorescència o flor amb:

!__! 00+ només flors marcents

!__! 0++ flors obertes i marcents

Sòl !__!

Altres: % èxit positiu:

Dades morfomètriques:

Floració simultània !__!

!__! 00+b con fiori appassiti e bacche acerbe

Floració escalonada !__!

!__! 00+fr flors marcents i fruits immadurs

!__! centrípeta

!__! 000b con bacche mature

!__! centrífuga

!X! 000fr fruits madurs

!__! basal

!X! 000sd semi dispersats

!__! ++0 flors tancades i obertes

Causa:

% de població productora de llavors: Recollida de: Planta !X!

5001-

Estat fenològic predominant

% plàntules:

!X! Llavors

!__! Esqueixos !__! Altres:

)

Tipus:

!__! altres: Tipus de material recol·lectat: Mostra d'herbari: Planta en test:

Si !X! No !__! Si !__! No !X!

Fruit test preliminar: Si !__! No !X! Condicions fitosanitàries de la població:

Estat de conservació de la població:

Mat. biologia molec.: Si !__! No !X!

Mat. cariologia:

Riscs i factors d'amenaça: Urbanització i industrialització

Si !__! No !X!

Banc de llavors del sòl: Si !__! No !X! Altres: Nota:

Pre-tractaments:

!__! Sense recol·lecta !__! Necessària nova recol·lecta !__! Necessari cultiu per determinació taxonòmica

Pàgina 1

Project title:

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project RECORDS DATABASE (Catalan language) Riu Manol FITXA RECOL·LECCIÓ GERMOPLASMA N.

ID progressiu:

Data ingrès:

Taxon: Silene sennenii

Operador:

Flora utilitzada per la determinació:

Dades poblacionals: Codi de població: Estat: Espanya Regió: Catalunya Província: Girona Comarca: Alt Empordà Localitat: Riu Manol (Lladó?) UTM: 4679300 499900 N ° ' '' E ° ' '' Instrument utilitzat: Precissió de les dades: Alçada: mitjana ( min max ) Inclinació: mitjana ( min max ) Exposició: mitjana ( de a Litologia: Superfície roques ( % ) : Superfície pedres ( % ) : Classif. pedològica:

Horitzonts: Drenatge: 1 2 3 4 5 Bioclima: Termotipus: Ombrotipus: Tipus de vegetació:

!__! Altres: _____ % Especificar _________________________ Data i hora de recol·lecció: Temp. (T°C):

21/08/08 Tarda Humitat Relativa (%): P. atmosfèrica (hPa-mbar)

~25ºC ~60% Condicions meteorològiques:

!__! Camp. pH: _____ Coerenza: 1 2 3 4 5

Codi Corine:

Codi Hàbitat:

Sol i núvols amb força vent Mètode de mostreig:

Àrea mostrejada (m2): 3000 Àrea població (m2): 5000

!X! Regularment distribuït per tota la superfície

Macropoblació !__!

!__! Al centre de la població

N. Micropoblacions !__!

!__! Al llarg d'una línia transversal

Estructura de la població: !X! Individus aillats (1) !__! Petits grups (2) !__! Grups (3) !__! Colònies (4) !__! Població (5)

!__! Al llarg d'una línia marginal !__! Altres:

N. individus presents: 1-5!_! 10000!_! >10000!_!

6-10!_!

11- 50!_!

51-100!X!

101-250!_!

251-500!_!

)

Dimensions (m2): Fitxa Demogràfica: Si

501-1000!_!

Ref. cartográfica: SI No N. de lots d'aquesta recol·lecció:

1001-2500!_!

2501-5000!_!

5001-

Material recol·lectat:

N. de llavors/fruit:

!X! Llavors

!__! Bulbils

N. de llavors/individu:

% plantes joves:

!__! 000 sense flors

!__! Fruits

!__! Bulbs

N. de llavors col·lectades/individuo:

% plantes adultes:

!__! +00 només flors tancades

N. individu sobre els quals s'ha fet la recol·lecta:

% plantes mortes:

!__! ++0 flors tancades i obertes

Causa:

!__! +++ flors tancades, obertes i marcents

!__! Pol·len

!__! Rizomes

!__! Espores

!__! Tubercles

!__! Esqueixos !__! Altres: Estat de la llavor:

Estat fenològic predominant

% plàntules:

!__! 0++ flors obertes i marcents

% de població productora de llavors: Recollida de: Planta !X!

Humit !__!

Sec !X!

N. de llavors proba del tall:

Sòl !__!

Inflorescència o flor amb:

Altres: % èxit positiu:

Dades morfomètriques:

!__! 00+b con fiori appassiti e bacche acerbe

Floració escalonada !__!

!__! 00+fr flors marcents i fruits immadurs

!__! centrípeta

!__! 000b con bacche mature

!__! centrífuga

!X! 000fr fruits madurs

!__! basal !__! apical Disseminació: Autocora !__! Barocora !X! Anemocora !__! Zoocora !__! Hidrocora !__! Pirocora !__! Igronastica !__! Balistocora !__! Atelecora !__! Policora !__! Altres !__!: Situada en un espai protegit: SI !__! NO !X!

!__! 00+ només flors marcents

Floració simultània !__!

Tipus:

!X! 000sd semi dispersats !__! altres: Tipus de material recol·lectat: Mostra d'herbari: Planta en test:

Si !__! No !X! Si !__! No !X!

Fruit test preliminar: Si !__! No !X! Condicions fitosanitàries de la població:

Mat. biologia molec.: Si !__! No !X!

Estat de conservació de la població:

Mat. cariologia:

Riscs i factors d'amenaça: Obres freqüents, deixalles, llum artificial nocturna

Si !__! No !X!

Banc de llavors del sòl: Si !__! No !X! Altres:

Nota:

Pre-tractaments:

!__! Sense recol·lecta !__! Necessària nova recol·lecta !__! Necessari cultiu per determinació taxonòmica

Pàgina 1

Project title:

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project RECORDS DATABASE (Catalan language) Mare de Déu del Mont FITXA RECOL·LECCIÓ GERMOPLASMA

ID progressiu:

Data ingrès:

Taxon: Peucedanum schottii

Operador:

Flora utilitzada per la determinació:

Dades poblacionals: Codi de població: Estat: Espanya Regió: Catalunya Província: Girona Comarca:Alt Empordà Localitat: Albanyà, Mare de Déu del Mont UTM: 475850E 4678514N N ° ' '' E ° ' '' Instrument utilitzat: Precissió de les dades: 10m Alçada: mitjana 1124 ( min max ) Inclinació: mitjana ( min max ) Exposició: mitjana N ( de a ) Litologia: Superfície roques ( % ) : Superfície pedres ( % ) : Classif. pedològica: Calcari

Horitzonts: Drenatge: 1 2 3 4 5 Bioclima: Termotipus: Ombrotipus: Tipus de vegetació:

!__! Altres: _____ % Especificar _________________________ Data i hora de recol·lecció: Temp. (T°C):

Humitat Relativa (%):

12:30 – 13:15 P. atmosfèrica (hPa-mbar)

!__! Camp. pH: _____ Coerenza: 1 2 3 4 5

Codi Corine:

Condicions meteorològiques:

Sol i núvol Mètode de mostreig:

Codi Hàbitat:

Àrea mostrejada (m2):

!X! Regularment distribuït per tota la superfície

Macropoblació !__!

!__! Al centre de la població

N. Micropoblacions !__!

!__! Al llarg d'una línia transversal

Estructura de la població: !X! Individus aillats (1) !X! Petits grups (2) !__! Grups (3) !__! Colònies (4) !__! Població (5)

!__! Al llarg d'una línia marginal !__! Altres:

N. individus presents: 1-5!_! 6-10!_! 10000!_! >10000!_! Uns 270 Material recol·lectat:

11- 50!_!

51-100!_!

101-250!_!

N. de llavors/fruit: 2

251-500!X!

Àrea població (m2): Dimensions (m2): Fitxa Demogràfica: Si

501-1000!_!

Ref. cartográfica: SI No N. de lots d'aquesta recol·lecció:

1001-2500!_!

2501-5000!_!

5001-

Estat fenològic predominant

% plàntules:

!__! 000 sense flors

!X! Llavors

!__! Bulbils

N. de llavors/individu:

% plantes joves:

!__! Fruits

!__! Bulbs

N. de llavors col·lectades/individuo:

% plantes adultes:

N. individu sobre els quals s'ha fet la recol·lecta:

% plantes mortes:

!__! ++0 flors tancades i obertes

Causa:

!__! +++ flors tancades, obertes i marcents

Inflorescència o flor amb:

!__! 00+ només flors marcents

!__! Pol·len

!__! Rizomes

!__! Espores

!__! Tubercles

!__! Esqueixos !__! Altres: Estat de la llavor:

100

!__! 0++ flors obertes i marcents

% de població productora de llavors: Recollida de: Planta !X!

Humit !__!

Sec !X!

N. de llavors proba del tall:

!__! +00 només flors tancades

Sòl !__!

Altres: % èxit positiu:

Dades morfomètriques:

Floració simultània !X!

!__! 00+b con fiori appassiti e bacche acerbe

Floració escalonada !__!

!__! 00+fr flors marcents i fruits immadurs

!__! centrípeta

!X! 000b con bacche mature

!__! centrífuga

!X! 000fr fruits madurs

!__! basal

!__! 000sd semi dispersats !__! altres:

!__! apical Disseminació: Autocora !__! Barocora !X! Anemocora !__! Zoocora !__! Hidrocora !__! Pirocora !__! Igronastica !__! Balistocora !__! Atelecora !__! Policora !__! Altres !__!:

Tipus de material recol·lectat:

Situada en un espai protegit: SI !__! NO !X!

Planta en test:

Tipus:

Mostra d'herbari:

Si !__! No !X! Si !__! No !X!

Fruit test preliminar: Si !__! No !X! Condicions fitosanitàries de la població:

Estat de conservació de la població:

3 3

4 4

Mat. biologia molec.: Si !__! No !X!

Mat. cariologia:

Riscs i factors d'amenaça: Depredació per part del insectes i freqüentació de les persones

Si !__! No !X!

Banc de llavors del sòl: Si !__! No !X! Altres:

Nota:

Pre-tractaments:

!__! Sense recol·lecta !__! Necessària nova recol·lecta !__! Necessari cultiu per determinació taxonòmica

Pàgina 1

Project title:

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project Generalitat de Catalunya Departament de Medi Ambient i Habitatge COMMUNICATION & PRESS PRESS RELEASE (Catalan language) Medi Ambient amplia de 5 a 182 les espècies de flora en perill d’extinció i vulnerables a Catalunya La direcció general del Medi Natural ha presentat avui a Blanes el Catàleg de Flora Amenaçada de Catalunya Implica un salt quantitatiu molt important, ja que amb l’anterior legislació només hi havia cinc espècies protegides en tot el territori català El Departament de Medi Ambient i Habitatge ha presentat avui, en el marc de les Primeres Jornades Catalanes de Conservació de Flora que es fan a Blanes (la Selva), el Catàleg de Flora Amenaçada de Catalunya, el decret del qual serà aprovat a curt termini pel govern de la Generalitat. A la presentació hi ha assistit la directora general del Medi Natural, Núria Buenaventura. El catàleg inclou 59 espècies “en perill” i 123 espècies “vulnerables”, un fet destacat ja que amb l’anterior legislació només hi havia protegides a tot el territori català 5 espècies. El catàleg inclou, entre altres, espècies pertanyents al grup de les orquídees, de les falgueres, espècies com la flor de neu (Leontopodium alpinum), l’estàquia marítima (Stachys maritima), algunes espècies de lliris i de la família de les gencianes. El decret que avui s’ha presentat i que crea el primer Catàleg de Flora Amenaçada de Catalunya recull les espècies i subespècies amenaçades que es classifiquen en les categories de “en perill d’extinció” o de “vulnerables” en funció del seu estatus de conservació i grau d’amenaça. El decret estableix que la direcció general del Medi Natural ha d’elaborar i aprovar plans de recuperació per a les espècies “en perill d’extinció” i dur a terme plans de conservació per a les espècies “vulnerables”. Project title:

La catalogació com a espècie o subespècie amenaçada comporta la prohibició de dur a terme diverses actuacions. Amb les espècies catalogades està prohibida qualsevol actuació no autoritzada amb el propòsit o resultat de destruirles, mutilar-les, tallar-les o arrancar-les, així com la recol. lecció de les seves llavors, pol.len o espores. Tampoc no es permet posseir-les, transportar-les, comercialitzar-les, exposar-les a la venda o importar-les, tant si es tracta d’exemplars vius o morts, així com els seus propàguls o restes. Així mateix, no està permès alterar-ne l’hàbitat afectant negativament les seves poblacions. Les espècies catalogades també s’han de tenir en compte en els tràmits d’avaluació d’impacte ambiental de qualsevol infrastructura o procés de transformació del territori que pugui tenir incidència en la conservació de l’espècie. S’ha de tenir en consideració l’impacte sobre les espècies o subspècies del catàleg i evitar-ne l’impacte o adoptar les mesures correctores. Quan aquestes no siguin possibles, cal adoptar les mesures de compensació que siguin adients, de forma que l’estat de conservació de l’espècie o subspècie sigui igual o superior a la situació inicial. Les infraccions seran sancionades d’acord amb el que estableix la Llei estatal 42/2007, de 13 de desembre, del Patrimoni Natural i de la Biodiversitat, sense perjudici de l’obligació de rescabalament dels danys i perjudicis a càrrec de qui en sigui responsable. En el termini de sis mesos a comptar des de l’entrada en vigor d’aquest Decret, els productors o particulars que tinguin material vegetal viu de les espècies o subespècies catalogades ho han de notificar a la Direcció General del Medi Natural. D’aquesta manera, la Direcció podrà pot autoritzar la seva tinença sempre i quan no s’afecti la conservació de l’espècie. Les col.leccions científiques adscrites a museus, centres de recerca o universitats, que incloguin material viu o exemplars d’herbari de les espècies incloses als annexos del present Decret també ho han de notificar a la Direcció General del Medi Natural en el termini d’un any per tal que pugui autoritzar-ne la seva tinença.

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project APPENDIX 1 Espècies catalogades com a “en perill d’extinció”. PTERIDÒFITS Asplenium marinum Asplenium sagittatum [=Phyllitis sagittata] Botrychium matricariifolium Marsilea quadrifolia Marsilea strigosa Pellaea calomelanos Thelypteris palustris Woodsia pulchella [=Woodsia glabella subsp. pulchella] ESPERMATÒFITS ANGIOSPERMES Luronium natans Sternbergia colchiciflora Hydrocotyle vulgaris Peucedanum schottii Seseli farrenyii Anthericum ramosum Simethis mattiazzi Sonchus crassifolius Taraxacum stenospermum Cerinthe glabra Lappula deflexa Boleum asperum Rorippa amphibia Gypsophila tomentosa [=Gypsophila perfoliata subsp. ilerdensis] Silene sennenii [=Silene italica subsp. sennenii] Maianthemum bifolium Erica vagans Phyllodoce caerulea Gentiana angustifolia subsp. angustifolia [=Gentiana acaulis subsp. angustifolia] Erodium sanguis-christi Erodium foetidum [=Erodium foetidum subsp. crispum] Hypericum elodes Hydrocharis morsus-ranae Project title:

Hippuris vulgaris Dracocephalum austriacum Scutellaria galericulata Stachys maritima Gagea pratensis Kosteletzkya pentacarpa Corallorhiza trifida Cypripedium calceolus Epipogium aphyllum Orchis spitzelii Plantago cornuti Limonium catalaunicum Limonium costae Limonium vigoi Oplismenus undulatifolius Polygonum romanum subsp. gallicum Microcnemum coralloides Aquilegia paui [=A. vulgaris subsp. paui] Delphinium bolosii [=Delphinium fissum subsp. bolosii] Ranunculus lingua Ranunculus nodiflorus Reseda jacquinii subsp. litigiosa [=Reseda phyteuma subsp. jacquinii] Spiraea crenata subsp. parvifolia [=Spiraea crenata] Salix foetida [=Salix arbuscula subsp. foetida] Chrysosplenium alternifolium Daphne alpina Verbena supina Zygophyllum album

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project APPENDIX 2 Espècies catalogades com a “vulnerables” PTERIDÒFITS Asplenium obovatum subsp. obovatum Asplenium seelosii subsp. catalaunicum Dryopteris remota [=Dryopteris carthusiana subsp. remota] Dryopteris mindshelkensis [=Dryopteris villarii subsp. submontana] Equisetum fluviatile Cosentinia vellea [=Cheilanthes vellea] Isoetes setaceum [=Isoetes setacea] Isoetes velatum subsp. velatum [=Isoetes velata subsp. velata] Isoetes durieui Lycopodium clavatum Diphasiastrum alpinum [=Lycopodium alpinum] Woodsia alpina ESPERMATÒFITS ANGIOSPERMES Allium pyrenaicum Ferula loscosii Pimpinella tragium Silaum silaus Arisarum simorrhinum [=Arisarum vulgare subsp. simorrhinum] Centaurea podospermifolia [=Centaurea lagascana subsp. podospermifolia] Hieracium queraltense Hieracium recoderi Hieracium vinyasianum Leontopodium alpinum subsp. alpinum Scorzonera humilis Senecio auricula subsp. sicoricus [=Senecio auricula var. sicoricus] Senecio aquaticus Berberis vulgaris subsp. seroi Lithodora oleifolia [=Lithospermum oleifolium] Project title:

Rochelia disperma subsp. retorta Arabis soyeri subsp. soyeri Cardamine parviflora Cochlearia pyrenaica [=Cochlearia officinalis subsp. pyrenaica] Draba fladnizensis Maresia nana Matthiola valesiaca [=M. fruticulosa subsp. valesiaca] Moricandia moricandioides subsp. cavanillesiana Succowia balearica Callitriche platycarpa Lonicera biflora Arenaria conimbricensis subsp. viridis Arenaria fontqueri subsp. cavanillesiana Minuartia villarii Petrocoptis pardoi [=Petrocoptis crassifolia subsp. montsicciana = Petrocoptis montsicciana] Saponaria bellidifolia Silene ramosissima Silene sedoides Halimium halimifolium Cneorum tricoccon Carex grioletii Carex lasiocarpa Carex limosa Scirpus sylvaticus Drosera longifolia Elatine alsinastrum Elatine macropoda [=Elatine hydropiper subsp. macropoda] Erica tetralix Antirrhinum pertegasii [=Antirrhinum sempervirens subsp. pertegasii] Chaenorhinum reyesii [=Chaenorhinum rubrifolium subsp. reyesii] Linaria oblongifolia subsp. aragonensis Melampyrum catalaunicum [=Melampyrum nemorosum subsp. catalaunicum] Pedicularis comosa subsp asparagoides Pedicularis tuberosa Scrophularia pyrenaica Veronica scutellata Euphorbia dendroides Euphorbia palustris Astragalus penduliflorus

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project Ononis ornithopodioides Centaurium favargeri [=Centaurium quadrifolium subsp. parviflorum] Gentiana pneumonanthe Erodium celtibericum [=Erodium foetidum subsp. celtibericum] Erodium lucidum [=Erodium foetidum subsp. lucidum] Erodium neuradifolium [=E. malacoides subsp. aragonense] Erodium rupestre [=Erodium foetidum subsp. rupestre] Geranium lanuginosum [=Geranium bohemicum subsp. lanuginosum] Hypericum linariifolium Iris xiphium Juncus pyrenaeus [=Juncus balticus subsp. pyrenaeus] Triglochin bulbosum subsp. barrelieri Sideritis bubanii [=Sideritis hirsuta subsp. bubanii] Thymus loscosii Thymus willkommii Pinguicula dertosensis [=Pinguicula grandiflora subsp. dertosensis] Utricularia australis Utricularia minor Menyanthes trifoliata Najas marina Gymnadenia odoratissima Orchis palustris [=Orchis laxiflora subsp. palustris] Spiranthes aestivalis Plantago loeflingii Armeria fontqueri [=Armeria maritima subsp. fontqueri] Limoniastrum monopetalum Limonium bellidifolium Limonium latebracteatum Limonium densissimum Limonium geronense Limonium gibertii Limonium girardianum Limonium revolutum Limonium tournefortii Limonium tremolsii Polygala vayredae Rumex hydrolapathum Potamogetum praelongus Salsola soda Project title:

Salicornia emerici Sarcocornia perennis subsp. alpini [=Arthrocnemum perenne subsp. alpini] Delphinium montanum [=Delphinium elatum subsp. montanum] Thalictrum maritimum [=Thalictrum morisonii subsp. maritimum] Alchemilla pentaphyllea Prunus prostrata Callipeltis cucullaria Galium scabrum Salix daphnoides Salix lapponum Salix tarraconensis Saxifraga aretioides Saxifraga catalaunica [=Saxifraga callosa subsp. catalaunica] Saxifraga fragosoi [=Saxifraga hypnoides subsp. continentalis] Saxifraga genesiana [=Saxifraga geranioides subsp. genesiana] Saxifraga longifolia subsp. aitanica [=Saxifraga longifolia subsp. longifolia var. aitanica] Saxifraga vayredana Atropa baetica Tamarix boveana

3 de juny de 2008

Comunicació i Premsa Departament de Medi Ambient i Habitatge PREMSA.DMAH@GENCAT.NET Telèfon de contacte 93 444 51 30

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project NEWSPAPER El Punt, Països Catalans

NEWSPAPER Diari de Gerona

Lab’s Inauguration (Catalan language)

Project title:

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project Status

A) How will you carry out your project? Get down to detail: think about how you are going to plan, implement and develop your project. You may like to use a timetable outlining actions, responsibilities, targets and timing.

Status

1- Study of the best area for the collection, pending to assigned by a scientific of the University from Barcelona

August

Done

2- Seed Collection

September

Done

3- Conservation Process (dry, deshidratation and frozen)

starting in September and it will be finished depending on seed characteristics

Done

4- Gene Test to evaluate the potential of the seed

after seed processing

Done

5- Plant the seed in the JB Marinurtra

next spring.

Done

6- Analyze the evaluation of these species

during whole project

Description of item/service required All the process until frozen step of the “ Silene sennenii”

Only "Silene Sennenii"

Done

Status

Budget

Cost

Available

475 €

0€

Done

All the process until frozen step of the “ Peucedanum Schottii”

375 €

0€

Done

Gene test and plantation

350 €

0€

Done

Vania’ s travel costs to Marimurtra

375 €

111 €

264 €

Viviana’ s travel costs to Barcelona and Marimurta

400 €

548 €

-148 €

0€

40 €

-40 €

1,975 €

1,899 €

76 €

Final Book of the Project

Total

Project title:

“Seed Bank of two species in danger of extinction – “Peucedanum schottii & Silene sennenii”

Earthwatch Project by Viviana Lopes