SUNSHELL
SunShell C18, 5 μm SunShell C18, 5 μm Characteristics of SunShell C18, 5 μm Characteristics of SunShell C18, 5 μm C18 - 5 µm Core shell silica
Particle Pore Core shell silica
size diameter Particle Pore Specific 4.6 µmsurface 9 nm SunShell C18 size diameter area
SunShell C18
4.6 µm
90 m2/g
9 nm
C18 (USP L1)
Specific surface area Carbon 90 m2/g content
5.5 %
Carbon content Bonded 5.5 % phase
Bonded Maximum operating C18 (USP L1) End-capping phase pressure Maximum operating Available pH End-capping C18 Sunniest End-capping pressure 60 MPa or 8,570 range psi
C18
Sunniest End-capping
Available pH range
1.5 - 10
1.5 - 10
60 MPa or 8,570 psi
Comparison Can of retention using HPLC be used inand anyplate L1 method - but with improved performance. Comparison of retention and plate using HPLC 1 1
1
2
SunShell C18 5 µm
Column size: 150 x 4.6 mm
N6=20,000
Column size: 150Mobile x 4.6phase: mmCH OH/H O=75/25 3 2 Column size: 150 x 4.6 mm N 5 6=20,000 Mobile phase: CH3OH/H2O=75/25 Flow rate: 1.0 mL/min 4 Mobile phase: CH3OH/H2O=75/25 6 7 Temperature: 40 ºC Flow Flow rate:rate: 1.01.0 mL/min 5 mL/min 3 4 Sample: 1 = Uracil 6 Temperature: 40 ºC Temperature: 40°ºC N6=31,000 2 = Caffeine SunShell C18 2.6 µm Sample: 1 = Uracil 7 Sample: 1 = Uracil 3 = Phenol 2 5 N =31,000 2 = Caffeine 6 21 4 SunShell 2.6MPa µm 1 C18 4 = Butylbenzene 76 3 2 = Caffeine 3 = Phenol 2 5 5 = o-Terphenyl 21 MPa 4 6 4 = Butylbenzene 3 = Phenol 3 6 = Amylbenzene 5 = o-Terphenyl 4 = Butylbenzene 7 = Triphenylene N6=14,000 6 = Amylbenzene 6 MPa HPLC: Hitachi LaChrom ELITE 5 = o-Terphenyl 7 = Triphenylene (Tubing, 0.25 mm i.d.) =14,000 N 6 6 MPa HPLC: LaChrom ELITE 6 =Hitachi Amylbenzene (Tubing, 0.25 mm i.d.) 7 = Triphenylene HPLC: Hitachi ELITE (Tubing, 0.25 mm i.d.) Totally porous silica Core shell silica Core shellLaChrom silica 8 MPa 2 SunShell C18 5 µm 8 MPa 3
7
Sunniest C18, 5 μm SunShell C18, 2.6 μm SunShell C18, 5 μm Totally porous silica Core shell silica Core shell silica There is a little 2/g Specific surface area Sunniest Totally porous shell silica Core 150 m2/g 90shell m2/gsilica C18, 5silica μm 340 mSunShell C18,Core 2.6 μm SunShell C18, 5 μm difference of k between Sunniest C18,Retention 5 µm SunShell C18, 2.6 µm SunShell C18, 5 µm There is a little Retention Retention Retention Specific surface area 150 m2/gRetention 90 m2/gRetention 340 m2/g totally porous and core factor (k) time (tR) factor (k) time (t ) factor (k) difference of k between 2 time (tR) 2 2 R 90 m /g Specific surface area 340 m /g 150 m /g Retention Retention Retention Retention Retention Retention shell particles. There is a small difference 1) Uracil 1.70 0 1.34 1.30(k) 0totally porous and core time (tR) factor (k) time (tR) factor (k) time0(tR) factor Retention Retention Retention Retention Retention Retention of k between totally porous shell particles. 6) Amylbenzene time1.70 19.96 10.74 time (tR) 16.56 11.36 13.43 9.33 (k) (tR) factor (k) factor (k) time factor 1) Uracil 0 1.34 0 1.30 0 (tR) 6) AmylbenzeneRelative value of Amylbenzene 19.96 1) Uracil 1.70
100% 10.74 0
100% 83% 16.56 1.34 11.36
106% 13.43 0
67% 9.33 1.30
value of Amylbenzene 6)Relative Amylbenzene
100% 10.74
83%
67% 11.36
87% 13.43
9.33
67%
87%
100% 19.96
16.56 106%
Comparison between normal and semi-micro HPLC Comparison between normal and semi-micro35,150 HPLC Relative value of Amylbenzene
100%
100%
ORDERING INFO OF SUNSHELL
Flow cell Flow cell Normal Semi-micro
42,053 Response Sampling Tubing ID
Normal Sampling 0.1 secTubing 0.4 ID sec Response Sunshell C18, µm0.4 0.1 sec50.25 Semi-micro 0.1 sec 0.4 sec mmsec
Semi-micro 0.1 sec 0.40.05 sec sec0.130.05 mmsec
Semi-micro 0.05 sec
0.05 sec
0.13 mm 0 2
34,462 2.1 4.6 USP category 40,3203.0 30,564 34,462 41,043 34,052 40,320 30,564 Catalog no40,139 Catalog no Catalog no Catalog no Catalog no 41,043 34,05237,854 40,139 41,610 37,854 39,255CB3471 ----CB3371 41,610 L1 39,255 ----CB3381 CB3481
150 250
0.13 mm
1
106%
Inner diameter 42,053 35,150 (mm) 13,993 Length (mm)
0.25 mm
0.13 mm 0
83%
1 3
and core shell particles.
87% 0
2 4
13,993 1.0
3 5
4 6
5 6 7 Retention time/min 7 8 9
8
9
10
11
10
11
11