GC CHROMATOGRAMS | FOODS, FLAVORS, & FRAGRANCES
Fatty Acids Fatty Acids (Free) on Rtx®-200
1. 2. 3. 4. 5. 6. 7. 8. 9.
Fatty Acids (Free) on Stabilwax®-DA (30 m, 0.25 mm ID, 0.25 μm) No derivatization needed!
Peaks Acetic acid Propionic acid Isobutyric acid n-Butyric acid Isovaleric acid n-Valeric acid Isocaproic acid Caproic acid Heptanoic acid
1. 2. 3. 4. 5. 6. 7. 8. 9.
No derivatization needed!
Peaks Acetic acid Propionic acid Isobutyric acid n-Butyric acid Isovaleric acid n-Valeric acid Isocaproic acid Caproic acid Heptanoic acid
GC_CH00282 Column Sample Conc.: Injection Inj. Vol.: Inj. Temp.: Split Vent Flow Rate: Oven Oven Temp: Carrier Gas Flow Rate: Linear Velocity: Detector Notes
GC_CH00280 Column
Rtx®-200, 30 m, 0.25 mm ID, 0.25 μm (cat.# 15023) Free fatty acid standard ~10-20 ng/μL
Sample Conc.: Injection Inj. Vol.: Inj. Temp.: Oven Oven Temp: Carrier Gas Linear Velocity: Detector Notes
0.8 μL split 250 °C 40 mL/min 90 °C H2, constant flow 1.4 mL/min 40 cm/sec FID @ 250 °C FID sensitivity: 4 x 10-11 AFS
Stabilwax®-DA, 30 m, 0.25 mm ID, 0.25 μm (cat.# 11023) Free acid standard ~10-20 ng/μL 1.0 μL split (split ratio 50:1) 250 °C 145 °C H2, constant pressure 40 cm/sec FID @ 250 °C FID sensitivity: 2 x 10-11 AFS
Fatty Acids (Free) on Stabilwax®-DA (30 m, 0.32 mm ID, 0.25 μm)
4
2
1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13.
Great for low to high molecular weight fatty acids.
5 6
3
7
1 8 Column 9
Sample Diluent: Conc.: Injection Inj. Vol.: Liner: Inj. Temp.: Purge Flow: Oven Oven Temp: Carrier Gas Flow Rate: Detector
10 11
12
Peaks C4:0 Butyric acid C6:0 Caproic acid C8:0 Caprylic acid C10:0 Capric acid C12:0 Lauric acid C14:0 Myristic acid C16:0 Palmitic acid C18:0 Stearic acid C18:1 Oleic acid C18:2 Linoleic acid C18:3 Linolenic acid C20:0 Arachidic acid C22:0 Behenic acid Stabilwax®-DA, 30 m, 0.32 mm ID, 0.25 μm (cat.# 11024) Free fatty acid mix Methanol 100 ppm 1 μL splitless (hold 0.25 min) Laminar cup splitter 250 °C 75 mL/min 40 °C to 250 °C at 10 °C/min (hold 15 min) H2, constant flow 6 mL/min FID @ 250 °C
13
GC_FF00653
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