Fig. 4 Comparison between silica-based columns KW-803 & KW-802.5 and polymer-based column SB-803 HQ
Fig. 4-1 Comparison of chromatograms
2
3
1
Sample : 1. Poly(ethylene glycol) 2. Poly(ethylene glycol) 3. Poly(ethylene glycol) 4. Poly(ethylene glycol) 5. EG, Ethylene glycol
5 4
SB-803 HQ 2
1
3
4
5
KW-803
1
2
5 3
4
KW-802.5 10
15
Columns Eluent Flow rate Detector Column temp.
: : : : :
20
Shodex OHpak SB-803 HQ or PROTEIN KW-802.5 or KW-803 (8.0mmID x 300mm each) H2O 0.5mL/min Shodex RI 30째C
Fig. 4-2 Comparison of calibration curves 10000 KW-802.5
KW-803
MW
1000
SB-803 HQ 100
10 16
25 min
18
20 22 Elution time (min)
24
-4-
(MW: 20,000) (MW: 4,000) (MW: 1,000) (MW: 400) (MW: 62)