Relevant equipment Automated work stations. Microarray hybridization and reading system. DHPLC. Real time PCR. Sequencer. Pyrosequencer.
Range of services Extracting and storing DNA from different sources, such as whole blood, plasma, blood serum, leucocytes, saliva and other body fluids, hair (with and without roots), tissue from biopsies and necropsies, paraffinembedded tissue, recent bone tissue and from archaeological sites, plant tissue, micro-organisms and processed and non-processed food. Separating and storing leucocytes to extract high-molecular-weight DNA and lymphocyte for their subsequent transformation. Collecting nuclear family trios. Collecting DNA samples of different pathologies. Collecting DNA samples of individuals without known pathologies, from different geographical origins. The DNA Bank is in contact with other biobanks and researchers and/or clinics. This can help with obtaining DNA from a wide range of pathologies and locations around the world. The DNA Bank has a wide catalogue of services, mainly relating to neuro-degenerative diseases (prion diseases, Alzheimer’s and Parkinson's), oncobiology (breast cancer and head & neck cancer), obesity and pharmacogenetics. Biological Identification and Kinship Service for humans and animal species, such as dogs and cattle. Training by means of specific courses. Educational support for various degrees and Master’s degrees.
Instrumental techniques used Manual and robotic extracting and purifying of nucleic acids. Managing collections of nucleic acids and mononuclear cells for their subsequent transformation into cell lines. PCR and real time PCR. Detecting mutations based on DHPLC, SSCPs, pyrosequencing and analysis of DNA sequences and methylation. Microarray hybridization and reading.
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