WORLD PRECISION INSTRUMENTS
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QUICK START GUIDE
NANOLITER2010
Nanoliter Injector with SMARTouch™ Controller
Serial No._____________________
www.wpiinc.com 061217
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Nanoliter 2010 with SMARTouch™ Controller Get started with your Nanoliter 2010 and SMARTouch™ Controller as quickly as possible using these step by step instructions. NOTE: This Quick Start Guide assumes that the operator back filled a micropipette with mineral oil. For instructions on how to prepare the micropipette, see the Nanoliter2010 Instruction Manual page 7. 1. Connect the AC power cord to the power supply unit and connect the power supply unit to the MICRO2T controller. Plug the AC power cord into a wall outlet.
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Connect the Nanoliter adapter cable to the output of channel 1 of the SMARTouch™ controller and the RJ-11 connector of the Nanoliter2010 to the RJ-11 female connector on the adapter. Make sure the embossed arrow on the connector is facing upwards for proper pin alignment.
Adapter Cable connects to the SMARTouch™ controller
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Nanoliter 2010 Injector connection to the adaper. 1
3. Power on the unit. The following screen appears.
4. Touch the Initial Splash Screen anywhere. The MAIN MENU appears. Touch DISPLAY ALL CHANNELS.
5. The syringe status screen appears. Press anywhere in the PUMP1 Display Area to make Pump1 the active pump.
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Nanoliter 2010 with SMARTouch™ Controller 6. Touch the Configure button.
7. Press the Type box to bring up the Syringe Selection Screen.
8. In the Syringe Selection Screen, press the DWN key until you see the NL (Nanoliter 2010) type and press it. It will become highlighted. Then press Back to return to the Pump Configuration Screen
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9. The CONFIGURATION screen appears. Touch the Volume Target display.
10. The Volume Target screen appears. Use the numerical keypad to enter 2500 nL. Touch ENTER to set the volume and return to the CONFIGURATION screen.
11. Touch the DELIVERY RATE display.
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Nanoliter 2010 with SMARTouch™ Controller 12. The Volume Rate screen appears. Use the numerical keypad to enter a desired injection rate in nL /sec. In this example, 500 nl/sec was entered. Touch ENTER to set the delivery rate and return to the CONFIGURATION screen.
13. The Configuration screen shows that a target volume of 2500 nL was entered and a delivery rate of 500 nL/s was entered. Touch BACK to return to the syringe status {Home} screen.
14. Verify that the arrow inside the syringe is yellow and pointing in the injection direction. If the arrow is not red and pointing to the left, touch DIRECTION to change it. Touch the RUN button to start the injection.
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15. The injection will run until 2500 nL is injected and will then halt automatically. Even though no injection actually happened, this will place the metal needle plunger about 13.6 mm from the home (fully retracted) position. At this point install the back filled micropipette on to the Nanoliter2010 injector as shown below.
16. Press the DIRECTION button to change the direction to Withdraw. The arrow turns green and points towards the back of the syringe depiction.
17. Press the CONFIGURE button.
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Nanoliter 2010 with SMARTouch™ Controller 18. Press the Volume Target area to bring up the Volume Target screen.
19. Use the keypad to enter the desired aliquot volume to be pulled into the micropipette. We will enter 200 nL and press Enter.
20. Press the Delivery Rate area.
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21. Use the keypad to set the rate to 50 nL/sec and press Enter.
22. Press Back to go to the main Syringe information display.
23. Next place the micropipette tip into the aliquot to be loaded and press RUN. This will pull 200 nL into the micropipette.
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Nanoliter 2010 with SMARTouch™ Controller 24. When the injector finishes, press the direction button to set it to Infuse. The arrow will turn Red.
25. Now the micropipette can be placed at the desired injection location and after setting the desired injection volume, the injection can be done by pressing the Run button.
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