CENTRALIZED RESEARCH CORE FACILITY CENTER FOR MOLECULAR TOXICOLOGY COLLEGE OF PHARMACY UNIVERSITY OF RHODE ISLAND SUPPORTED BY GRANT # 5P20GM10343013 FROM THE NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES OF THE NATIONAL INSTITUTES OF HEALTH
Rhode Island IDeA Network of Biomedical Research Excellence The RI–INBRE Program is being supported through a competitive continuation award from the National Institute of General Medical Sciences, National Institutes of Health. The program was initially funded through a 3-year award in October 2001 under the old name RI-BRIN. The Program is housed at the University of Rhode Island under the auspices of Center for Molecular Toxicology, College of Pharmacy. It is directed by Professor Zahir Shaikh and coordinated by Professor David Rowley. The purpose of the grant is to enhance biomedical research at universities and colleges in Rhode Island, and to support and develop talented individuals committed to research careers in academia, healthcare, and the pharmaceutical and biotechnological companies.
Centralized Research Core Facility The RI-INBRE Centralized Research Core Facility inaugurated in July 2003, is being supported by the Rhode Island IDeA Network of Biomedical Research Excellence (RI-INBRE) grant and by the participating institutions that include: University of Rhode Island, Brown University, Rhode Island College, Providence College, Salve Regina University, Bryant University and Roger Williams University. This facility is located in the College of Pharmacy at the University of Rhode Island’s Kingston campus. It is equipped with instrumentation for biomedical, pharmaceutical and biotechnological research. The facility is accessible to researchers from both academia and private companies within the state. Dr. Aftab Ahmed, manage the laboratory and assisted by Nathan Nous. They are available to consult, assist in the use and operation of the instruments and to analyze samples on a fee-forservice basis.
Equipment Resources Proteomics & Genomics • • • • • • •
MALDI-TOF MS/MS QSTAR ESI-TOF LC/MS Protein sequencer 2-D Electrophoresis System DNA Microarray Reader Simon Western blotting System Real Time –PCR
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Analytical HPLC Preparative HPLC Ultra-HPLC FPLC
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Microwave Digestion System ICP-MS
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Biological Safety Hood Incubators Low, High, and Ultra Speed Centrifuge Flow Cytometer (FACS) Cell Viability Analyzer
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CD Spectropolarimeter UV/VIS Spectrophotometer NanoDrop Spectrometer Fluorescence Spectrometer FT-IR Spectrometer Multimode Microplate Reader
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Digital Fluorescence Microscopes Variable Mode Imager Infrared Imager Image Scanner Whole animal imager
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Liquid Scintillation Counter Gamma Counter Freeze Dryer & SpeedVac Autoclave Cryostat Rotary Evaporator
Chromatography
Elemental Analysis
Cell Culture & Cell Fractionation
Spectroscopy
Imaging
General Equipment
Highlights •
Determine the N-terminal amino acid sequence of protein and peptide by ABI Procise gas phase Protein Sequencer.
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Use the Becton Dickinson (BD) FACSVerse Fluorescence Activated Cell Sorter to count and measure various cell characteristics such: relative size, granularity, and fluorescence of many cell populations.
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Perform high resolution mass analysis with the Applied Biosystems QSTAR Elite ESI-TOF LC/MS/MS Mass Spectrometer.
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Measures multiple metals at trace and ultra trace levels using Thermo Electron Inductively Coupled Plasma Mass Spectrometer (ICP-MS)
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Analyze proteome using Agilent Offgel fractionators, a novel isoelectric focusing technique to achieve excellent pl-based fractionation in solution
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Perform mass analysis of proteins, peptides, sugar and oligo-nucleotides.
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Image multicolor fluorescence, chemiluminescence, and filmless autoradiography with the Amersham Biosciences Typhoon Variable Mode Imager.
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Use infrared based Li-Cor Bioscience Imager for Westernblots, in-cell Westerns and protein gels.
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Perform DNA Microarray analysis using agilent Bioanalyzer and Microarray Scanner.
Visit www.uri.edu/inbre/corelab for instrument reservation, or Contact: Aftab Ahmed, Ph.D. Research Assistant Professor/Coordinator E-mail: aftab@mail.uri.edu Kim Andrews, B.A. Research Associate andrewskm@mail.uri.edu College of Pharmacy 7 Greenhouse Road, Room 405 Kingston, RI 02881 Tel: (401) 874-5731 Fax: (401) 874-2646