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Effects of Brassinosteroid on Antioxidant System in Salvia miltiorrhiza under Drought Stress IV.

CONCLUSION

At B1 treatment, the content of Pro increased to its peak, the activity of SOD and CAT also rose significantly, while the content of MDA hit its lowest point. Accordingly we conclude that BR at 01.mg/l can effectively enhance the resistance of S. miltiorrhiza under drought stress. Because of the synergies of antioxidant enzyme, we cannot just rely exclusively on a single index to evaluate the effects of BR on antioxidant system in S. miltiorrhiza under drought stress. Therefore, such as a plant hormone, when we undertake a comprehensive evaluation of the plant hormone, we must take account of the major of antioxidant system indicator[2, 11, 3031]. In summary, by comprehensive consideration of above three different concentration indicators, the 0.1mg/l BR treatment is the best choice to enhance the antioxidant ability of S. miltiorrhiza. The present study reveals that the level of antioxidant system (SOD, POD, CAT and Pro) increased in response to drought stress that was further improved by BR treatment. Therefore, it may be suggested that the ameliorated level of antioxidant system, at least in part, was responsible for the development of resistance against drought stress in S. miltiorrhiza seedlings. The increase in the degree of resistance due to the application of BR was reflected in the improvement of S. miltiorrhiza growth. This study provided the basic date for the indepth research of the role of BR in the amelioration of drought stress.

ACKNOWLEDGEMENTS This work was financially supported by the programs of agriculture science technology achievement transformation fund, China (2012GB2F000385), innovation fund for technology based firms, China (12C26215105863), the science and technology department of Sichuan province, China (2011ZO0006, 2012ZR0050), and innovation fund for technology based firms, Chengdu (11CXYB958JH-003).

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*Corresponding Author: Li Zhang

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