VWR COLLECTION PCR ENZYMES
Manufactured by Ampliqon for VWR International Europe
Denmark, Finland, Norway, Portugal and Spain
DEAR CUSTOMER
The aim of this catalogue is to provide you with a handy overview of our full VWR Collection PCR Enzymes available in Denmark, Finland, Norway, Portugal and Spain. FLEXIBILITY AND TAILORED SOLUTIONS Since 2008 VWR International has offered our PCR enzymes to European customers. We work closely together with Ampliqon, the Danish manufacturer of our VWR Collection PCR enzymes, to offer a wide range of high quality enzymes suitable for all DNA amplification purposes in use in your laboratory. The cooperation between VWR and Ampliqon allows us to tailor custommade solutions for individual customers at competitive prices. The same high quality and affordable prices are the basis of our comprehensive standard PCR enzyme product line.
SAMPLES We acknowledge that samples are the best way to put our quality to the test. Therefore, we maintain an extensive sampling programme, which permit you to try our enzymes in your specific amplification set-up. SUPPORT The VWR customer service has ready access to the Ampliqon support team, which facilitates convenient processing of requests and technical questions within 24 hours. The VWR Collection PCR enzymes is rapidly becoming a still more recognized life science brand in Europe and we hope that this catalogue shows that we are proud to be your supplier of high quality reagents. Kind regards VWR International Life Science Europe
TEXT Birgitte Vedelstorp Andersen LAYOUT Anne Mette Jensen [di’zain] IMPRINT VWR Norway and Ampliqon A/S, Skovlunde, Denmark 2010 PRINTER’S FROM Grafisk as, Kolding, Denmark This catalogue is for distribution in Denmark, Finland, Norway, Portugal and Spain
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CONTENTS
TAQ
4
Taq DNA Polymerase
4
Glycerol free
4
Mg free
4
Tween free
4
Taq DNA Polymerase Master Mix
6
Taq RedPOL DNA Polymerase
6
Taq DNA Polymerase Master Mix RED
7
HIGH FIDELITY
8
AccuPOL DNA Polymerase
8
Tween free
8
HOT START
10
TRUeHOT DNA Polymerase
10
TRUeHOT DNA Polymerase Master Mix
12
TRUeHOT DNA Polymerase Master Mix BLUE
13
Multiplex TRUeHOT DNA Polymerase Master Mix
14
NUCLEOTIDES
15
dNTP Mix
15
dNTP Set
15
VWR PRACTICAL INFORMATION 17 ABOUT AMPLIQON
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3
TAQ
TAQ DNA POLYMERASE TAQ DNA POLYMERASE
TAQ DNA POLYMERASE
VWR taq DNA Polymerase is suitable for routine PCR applications, which require high yield and reliable DNA amplification.
PRODUCT NUMBER
PRODUCT NUMBER
733-1300
250 units
Mg free and Key Buffer
733-1301
500 units
733-1311
500 units
FEATURES I A thermostable recombinant DNA polymerase I Enzyme isolated from Thermus Aquaticus I Molecular weight of approximately 95 kDa I Leaves an A’ overhang
733-1302
1000 units
733-1312
1000 units
733-1819
2500 units
733-1313
2500 units
733-1820
5000 units
733-2009
10000 units
733-1303
10000 units
DESCRIPTION I VWR Taq DNA Polymerase exhibits both a 5’ 3’ DNA polymerase and a 5’ 3’ exonuclease activity. I VWR Taq DNA Polymerase has no proofreading activity
Mg free and Extra Buffer
I
4
Alternative VWR taq combinations also available: Glycerol free Mg free Tween free Extra mM MgCl2
Tween free Glycerol free 733-1817
1000 units
733-1307
1000 units
733-1823
10000 units
20 mM MgCl2
733-1304
500 units
733-1305
1000 units
733-1826
1000 units
TAQ
COMPARISON BETWEEN VWR TAQ DNA POLYMERASE AND COMPETITORS
Enzyme dilution
Enzyme dilution
1x
2x
4x
6x
VWR
2x
4x
6x
2x
4x
6x
2x
4x
6x
2x
4x
6x
Competitor I
1x
2x
4x
6x
VWR
1x Competitor F
1x
2x
4x
6x
VWR
1x Competitor B
1x VWR
1x
2x
4x
6x
1x Competitor A
ENZYME DILUTION Comparison between VWR Taq DNA Polymerase and four competitors. Amplification of a 160 bp fragment of the human phenylalanine hydroxylase (PAH) gene. Each amplification has been conducted according to supplier’s instructions.
5
TAQ
TAQ DNA POLYMERASE MASTER MIX VWR Taq DNA Polymerase Master Mix is an alternative to Taq DNA Polymerase. Taq DNA Polymerase Master Mix offers reduced set-up time and eliminates the risk of contamination of reaction components. Fewer handling steps lead to better reproducibility, which makes Taq DNA Polymerase Master Mix suitable for standard tests.
FEATURES Premixed solution I Minimal optimization I Fewer pipetting steps I
DESCRIPTION Taq DNA Polymerase Master Mix is a ready to use 1.1 x or 2.0 x reaction mix. Taq DNA Polymerase Master Mix is composed of VWR Taq DNA Polymerase, NH4+ buffer system, dNTPs and magnesium chloride.
TAQ DNA POLYMERASE MASTER MIX PRODUCT NUMBER 1.1 x and 1.5 mM MgCl2 733-2540
500 reactions
733-1314
2500 reactions
1.1 x and 2.0 mM MgCl2 733-2541
500 reactions
733-1315
2500 reactions
2.0 x and 1.5 mM MgCl2 733-2542
500 reactions
733-1316
2500 reactions
2.0 x and 2.0 mM MgCl2
REDPOL TAQ DNA POLYMERASE VWR RedPOL Taq DNA Polymerase is an alternative to standard Taq DNA Polymerase. The red dye provides convenient identification of enzyme tube and confirms complete mixing.
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FEATURES The inert dye has no effect on downstream processes
I
DESCRIPTION VWR RedPOL Taq DNA Polymerase is added directly to the reaction mix.
733-2543
500 reactions
733-1317
2500 reactions
REDPOL TAQ DNA POLYMERASE PRODUCT NUMBER 733-1323
2500 units
733-1834
10000 units
TAQ
TAQ DNA POLYMERASE MASTER MIX RED VWR Taq DNA Polymerase Master Mix RED is an alternative to Taq DNA Polymerase Master Mix with the same advantages.The inert red dye and stabilizer are extra features, which allow for direct loading onto your agarose gel for analysis.
FEATURES No need for separate loading dye I Taq DNA Polymerase Master Mix RED is ready-made for electrophoresis and visualization I Fewer handling steps I
DESCRIPTION Taq DNA Polymerase Master Mix RED is a ready to use 1.1 x or 2.0 x reaction mix. Taq DNA Polymerase Master Mix RED is available in two final MgCl2 concentrations: 1.5 mM and 2.0 mM. Taq Polymerase Master Mix RED is composed of VWR Taq DNA Polymerase, NH4+ buffer system, dNTPs and magnesium chloride.
TAQ DNA POLYMERASE MASTER MIX RED PRODUCT NUMBER 1.1 x and 1.5 mM MgCl2 733-2544
500 reactions
733-1318
2500 reactions
1.1 x and 2.0 mM MgCl2 733-2545
500 reactions
733- 1319
2500 reactions
2.0 x and 1.5 mM MgCl2 733-2546
500 reactions
733-1320
2500 reactions
2.0 x and 2.0 mM MgCl2 733-2547
500 reactions
733-1321
2500 reactions
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HIGH FIDELITY
ACCUPOL DNA POLYMERASE VWR AccuPOL DNA Polymerase has proofreading abilities and is targeted at PCR amplications, which require high fidelity or blunt endings.
FEATURES AccuPOL DNA Polymerase is a thermostable enzyme I Processes <3 kb I
ACCUPOL DNA POLYMERASE PRODUCT NUMBER
DESCRIPTION AccuPOL DNA Polymerase exhibits both 5’ 3’ DNA polymerase activity and 3’ 5’ proofreading exonuclease activity. AccuPOL kits include an extra 25 mM MgCl2 concentration if higher MgCl2 is required for specific reactions.
733-1324
250 units
733-1325
500 units
733-1326
1000 units
Mg and Tween free 733-1328
250 units
733-1329
1000 units
VWR AccuPOL DNA Polymerase also comes in two alternative versions: Mg free and Tween free
FIDELITY COMPARISONS OF THERMOSTABLE DNA POLYMERASES USING THE LACIOZ ASSAY
ENZYME
ACCUPOL
PFU
TAQ
Error rate* (fidelity)
1.1 x 10-6
1.3 x 10-6
18.0 x 10-6
30
25
61
Elongation rate (bases/sec)
* The error rate equals mutation frequency per base per duplication.
8
9
HOT START
TRUEHOT DNA POLYMERASE VWR TRUeHOT DNA Polymerase has been designed to avoid spurious amplification and to diminish the formation of non-specific priming events during set-up. TRUeHOT DNA Polymerase allows for low target copy numbers and for multiplex PCR in a single reaction tube. FEATURES I Reaction set-up at room temperature INCREASED I Sensitivity I Specificity I Product yield I
Ideal for real time PCR applications (real time protocol included)
I
Suitable for multiplex applications
10
DESCRIPTION TRUeHOT Hot Start DNA Polymerase is a modified form of VWR Taq DNA Polymerase, which is activated by heat treatment. TRUeHOT DNA Polymerase is provided in an inactive state with no polymerase activity at room temperature due to a temperature dependent peptide inhibition of the DNA polymerase activity. Thus, during set up and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. TRUeHOT DNA Polymerase is activated by a 95째 C preincubation step.
TRUEHOT DNA POLYMERASE PRODUCT NUMBER 733-1843
500 units
733-1844
2500 units
733- 1845
10000 units
HOT START
VWR
Competitor Q
Competitor P
Competitor I
Competitor F
Competitor FS
Chemical modified hot start Taq
Antibody based hot start Taq
Antibody modified hot start Taq
Chemical modified hot start Taq
Genetic modified hot start Taq
Comparison with and without hot start activation: VWR TRUeHOT DNA Polymerase and competitors. Amplification of a 210 bp fragment of the phenylalanine hydroxylase (PAH) gene conducted according to supplierâ&#x20AC;&#x2122;s instructions.
AMPLIFICATION WITH AND WITHOUT HOT START Fluorescence (dRn) 4.7
10 units TRUeHOT, 100 ng DNA, hot start 5 units TRUeHOT, 100 ng DNA, hot start 10 units competitor Q, 100 ng DNA, hot start
3.7
5 units competitor Q, 100 ng DNA, hot start 10 units TRUeHOT, 100 ng DNA, no hot start
2.7
5 units TRUeHOT, 100 ng DNA, no hot start 10 units competitor Q, 100 ng DNA, no hot start 5 units competitor Q, 100 ng DNA, no hot start
1.7
0.7
-0.3 1
3
5
7
9
11
13
15
17
19 21 Cycles
23
25
27
29
31
33
35
Comparison with and without hot start activation: TRUeHOT Hot Start DNA Polymerase and competitor Q. Amplification has been conducted according to supplierâ&#x20AC;&#x2122;s instructions.
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HOT START
TRUEHOT DNA POLYMERASE MASTER MIX VWR TRUeHOT DNA Polymerase 2 x Master Mix is a ready to use mix that offers easy reaction assembly at room temperature prior to hot start PCR. Just add template and primers. TRUeHOT DNA Polymerase 2 x Master Mix is an alternative to TRUeHOT DNA Polymerase and is applicable for primer extensions and other amplification purposes.
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FEATURES I Minimal optimization I Convenient reaction set-up I Fewer pipetting steps I Consistent test results DESCRIPTION VWR TRUeHOT DNA Polymerase, NH4+ buffer system, dNTPs and magnesium chloride are present in TRUeHOT DNA Polymerase 2 x Master Mix.
TRUEHOT DNA POLYMERASE 2 X MASTER MIX PRODUCT NUMBER 733-2550
500 reactions
733-1847
2500 reactions
HOT START
TRUEHOT DNA POLYMERASE MASTER MIX BLUE
FEATURES Inactive at room temperature I Premixed 2 x solution I
TRUEHOT DNA POLYMERASE 2 X MASTER MIX BLUE PRODUCT NUMBER
DESCRIPTION VWR TRUeHOT DNA Polymerase 2 x Master Mix BLUE consists of TRUeHOT DNA Polymerase, dNTPs, a blue loading dye and magnesium chloride.
The blue loading dye facilitates direct gel loading and eliminates the need for separate loading dye for electrophoresis and visualization after PCR. TRUeHOT DNA Polymerase 2 x Master Mix BLUE is an alternative to TRUeHOT DNA Polymerase 2 x Master Mix with extra time saving advantages.
733-2010
500 reactions
733-1849
2500 reactions
TRUEHOT COMPARISON
Lane
1
2
3
4
5
Comparison with and without 15-minute hot start activation. TRUeHOT DNA Polymerase (lanes 1 and 2). TRUeHOT DNA Polymerase 2 x Master Mix BLUE (lanes 3 and 4). A DNA marker (lane 5).
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HOT START
MULTIPLEX TRUEHOT DNA POLYMERASE MASTER MIX Multiplex PCR is a method that enables amplification of two or more amplicons simultaneously in a single reaction tube. Multiplex TRUeHOT DNA Polymerase 2 x Master Mix is suitable for genotyping applications and various areas of DNA testing in research, forensic and diagnostic laboratories. FEATURES I Minimal optimization I Reduced set-up time I Minimizes contamination of component stock I Fewer handling steps I Preincubation at 95째 C INCREASED I Reproducibility I Specificity
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DESCRIPTION VWR Multiplex TRUeHOT DNA Polymerase Master Mix contains TRUeHOT DNA Polymerase. The polymerase is provided in an inactive state with no polymerase activity at room temperature due to a temperature dependent peptide inhibition of the DNA polymerase activity. This prevents the formation of primer-dimers and misprimed products during reaction set-up. The multiplex PCR buffer contains a balanced composition of salt and additives to ensure comparable efficiencies for annealing and extension of all primers in the reaction and facilitates the amplification of multiplex PCR products. Multiplex TRUeHOT DNA Polymerase Master Mix is provided with a PCR additive betaine solution that facilitates amplification of difficult templates by modifying the melting behavior of the DNA.
MULTIPLEX TRUEHOT DNA POLYMERASE MASTER MIX PRODUCT NUMBER 733-1851
250 reactions
733-1852
2500 reactions
NUCLEOTIDES
DNTP FEATURES I PCR amplification components I For DNA polymerization, DNA labelling and sequencing processes I High purity: > 98 % by HPLC I pH 7.5
DNTP MIX DNTP MIX Ready to use solution Mix of dATP, dCTP, dGTP and dTTP
PRODUCT NUMBER 25 mM of each dA, dC, dG and dT 733-1854 (2 x 1000 µl)
2000 µl
733-1363 (2 x 500 µl)
1000 µl
DNTP SET DNTP SET Set of dATP, DCTP, dGTP and dTTP
PRODUCT NUMBER 100 mM dA, dC, dG and dT 733-1364 (4 x 250 µl)
1 set
733-1855 (16 x 250 µl)
1 set
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PRACTICAL INFORMATION
FOR ENQUIRY, PURCHASE AND SUPPORT KINDLY CONTACT YOUR LOCAL VWR DISTRIBUTION PARTNER DENMARK VWR - Bie & Berntsen Transformervej 8 2730 Herlev Tel.: 43 86 87 88 Fax: 43 86 87 90 E-mail: info@dk.vwr.com http://dk.vwr.com FINLAND VWR International Oy Valimotie 9 00380 Helsinki Tel.: 09 80 45 51 Fax: 09 80 45 52 00 E-mail: info@fi.vwr.com http://fi.vwr.com NORWAY VWR International AS Haavard Martinsens vei 30 0978 Oslo Tel.: 02290 Fax: 815 00 940 E-mail: info@no.vwr.com http://no.vwr.com
PORTUGAL VWR International - Material de Laboratório, Lda Edifício Neopark Av. Tomás Ribeiro, 43- 3 D 2790-221 Carnaxide Tel.: 21 3600 770 Fax: 21 3600 798/9 E-mail: info@pt.vwr.com http://pt.vwr.com
For latest news, special offers and detailed info on VWR please go to: www.vwr.com
SPAIN VWR International Eurolab S.L. C/ Tecnologia 5-17 Llinars Park 08450 - LLinars del Vallès Barcelona Tel.: 902 222 897 Fax: 902 430 657 E-mail: info@es.vwr.com http://es.vwr.com
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ABOUT AMPLIQON
Ampliqon A/S is a Danish manufacturer of PCR enzymes and bioreagents. Ampliqon was established as a small scale PCR enzyme company in 2002. In 2009 we took over a comprehensive bioreagent product line. Today, Ampliqon is a recognized life science reagents supplier with focus on quality, small batch production, flexibility and good service.
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Our modern production facility is situated on the outskirts of Copenhagen with optimum laboratories and a highly skilled laboratory staff. We value our well-run cooperation with VWR International on VWR Collection PCR enzymes in Europe and custom-made Ampliqon labelled bioreagents in Denmark.
ABOUT AMPLIQON
PCR The Ampliqon science team has been an active party in the field of PCR from the first development of advanced PCR technologies to the PCR method prevailing today. Our expert knowledge of the important enzyme purification process and DNA amplification backs our day to day production procedures and laboratory team. We proudly manufacture reliable, high quality PCR enzymes and willingly share our product and technical knowledge with the VWR product specialists.
CUSTOM-MADE BIOREAGENTS Ampliqon manufactures more than 1000 custom-made bioreagents for Danish VWR customers. Our full VWR customer protocol archive holds about 10000 recipes. The proprietary customer protocols are tailored to fit the individual needs of each client and are shipped directly from Ampliqon to hospitals, biotech industries, research and educational institutions all over Denmark on a daily basis. We appreciate that VWR Denmark selected Ampliqon as their sole bioreagent supplier. VWR International is exclusive distributor of Ampliqon bioreagents.
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